FastQCFastQC Report
Fri 27 May 2016
SRR2140315_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140315_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4560214
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44500.042.840061
GTATCAA76250.032.2983861
TCAACGC84450.027.8993824
ATCAACG86150.027.3494423
CAACGCA86600.027.1119675
AACGCAG90750.026.0073556
TATCAAC93350.025.7683812
ACGCAGA107000.022.095877
CGCAGAG108100.021.8331858
GTACATG125550.021.6359271
TACATGG126400.020.843132
ACATGGG125650.020.2492623
TGCGCGA4201.4927307E-518.6733194
CATGGGG95350.018.5325364
GCAGAGT136900.017.299849
GTGGTAT27000.016.36381
GAGTACT69500.016.2736312-13
TATAGCG2801.9257823E-716.0694735
GAGTACA91900.016.0254821
CAGAGTA130500.015.90715710-11
Produced by FastQC (version 0.11.5)