##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140315_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4560214
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.983358894999228	31.0	31.0	34.0	30.0	34.0
2	32.08890481894051	33.0	31.0	34.0	30.0	34.0
3	31.824990011433673	31.0	31.0	34.0	30.0	34.0
4	35.89081301886271	37.0	35.0	37.0	35.0	37.0
5	35.82846002402519	37.0	35.0	37.0	35.0	37.0
6	35.86815596811904	37.0	35.0	37.0	35.0	37.0
7	35.8522183388762	37.0	35.0	37.0	35.0	37.0
8	35.848172037540344	37.0	35.0	37.0	35.0	37.0
9	37.6240266355921	39.0	37.0	39.0	35.0	39.0
10-11	37.528251744326035	39.0	37.0	39.0	35.0	39.0
12-13	37.48204349620434	39.0	37.0	39.0	34.5	39.0
14-15	38.73724347146866	40.0	38.0	41.0	34.0	41.0
16-17	38.67266580033305	40.0	38.0	41.0	34.0	41.0
18-19	38.66781230003679	40.0	38.0	41.0	34.0	41.0
20-21	38.731842175548834	40.0	38.0	41.0	34.0	41.0
22-23	38.69079539062513	40.0	38.0	41.0	34.0	41.0
24-25	38.596872183117455	40.0	38.0	41.0	34.0	41.0
26-27	38.45123601155608	40.0	38.0	41.0	34.0	41.0
28-29	38.323837988436225	40.0	38.0	41.0	34.0	41.0
30-31	38.20418279840065	40.0	38.0	41.0	34.0	41.0
32-33	38.01986157953333	40.0	37.0	41.0	33.0	41.0
34-35	38.10668528449082	40.0	38.0	41.0	33.0	41.0
36-37	38.070138287149035	40.0	37.5	41.0	33.0	41.0
38-39	38.033007399023035	40.0	37.0	41.0	33.0	41.0
40-41	37.96999488672924	40.0	37.0	41.0	33.0	41.0
42-43	38.00066966495502	40.0	37.0	41.0	33.0	41.0
44-45	38.03103595299531	40.0	37.0	41.0	33.0	41.0
46-47	37.97943201593217	40.0	37.0	41.0	33.0	41.0
48-49	37.88596593639123	40.0	37.0	41.0	33.0	41.0
50-51	37.78415630196515	40.0	37.0	41.0	33.0	41.0
52-53	37.50987506899972	40.0	36.0	41.0	32.0	41.0
54-55	37.4467677463045	39.0	36.0	41.0	32.0	41.0
56-57	37.27288339231133	39.0	35.5	41.0	31.5	41.0
58-59	37.09614007206983	39.0	35.0	41.0	31.0	41.0
60-61	36.94066165725953	39.0	35.0	40.0	31.0	41.0
62-63	36.68633365579691	38.0	35.0	40.0	31.0	41.0
64-65	36.31924892100698	38.0	35.0	40.0	30.5	41.0
66-67	36.06526323148401	37.0	34.5	40.0	30.0	41.0
68-69	35.7749946118569	37.0	34.0	39.5	30.0	41.0
70-71	35.49851478343289	36.0	34.0	39.0	30.0	41.0
72-73	35.20763372205775	36.0	34.0	39.0	30.0	40.0
74-75	34.884623615858445	35.0	34.0	38.5	29.0	40.0
76-77	33.64537406473485	34.5	32.0	36.5	28.0	39.0
78-79	34.26622759250452	35.0	33.5	37.0	29.0	39.0
80-81	34.20195501537326	35.0	34.0	36.5	29.0	39.0
82-83	33.93543058529781	35.0	33.5	36.0	29.0	37.5
84-85	33.70952913438835	35.0	33.0	36.0	29.0	37.0
86-87	33.51764432143218	35.0	33.0	35.0	29.0	37.0
88-89	33.35638667325472	35.0	33.0	35.0	29.0	36.0
90-91	33.23049984444232	35.0	33.0	35.0	29.0	36.0
92-93	33.206775662580455	35.0	33.0	35.0	29.0	36.0
94-95	33.20358588544318	35.0	33.0	35.0	29.0	35.5
96-97	33.19597833640947	35.0	33.0	35.0	29.0	35.0
98-99	33.30425378550855	35.0	33.0	35.0	29.5	35.0
100	32.186293116068335	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	8.0
20	31.0
21	176.0
22	739.0
23	2519.0
24	6436.0
25	13661.0
26	24465.0
27	39267.0
28	56428.0
29	75042.0
30	95476.0
31	119250.0
32	147360.0
33	187246.0
34	250222.0
35	344657.0
36	533397.0
37	904054.0
38	1121219.0
39	615634.0
40	22926.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.8854685328364	20.27130744302789	13.410445211562442	24.432778812573268
2	15.207395707826207	21.25160696639395	40.19159469401737	23.349402631762473
3	18.318061356177886	25.825733893701774	30.45664618829213	25.399558561828208
4	12.86753056171452	17.68508347990954	38.27407531995862	31.173310638417313
5	13.32643599620544	37.84728085129338	35.60074154414683	13.225541608354344
6	30.68057770973029	37.58795091633858	18.419025949220806	13.312445424710331
7	27.102850874980867	32.24054836023046	22.914165870285913	17.74243489450276
8	24.971722818271246	36.44076791133048	20.980967121279832	17.60654214911844
9	25.721336761827406	16.322435745340023	21.029320115240203	36.926907377592364
10-11	24.566972076310453	26.456587344365857	28.77802445236123	20.198416126962464
12-13	25.11968736554907	23.889920516888022	28.940900580542934	22.049491537019975
14-15	22.241738886547985	25.449828960558907	26.171167066922752	26.137265085970355
16-17	22.136264782101232	28.60042947654189	26.921951382641453	22.341354358715428
18-19	22.09082396593087	27.981586091655082	28.378437942694738	21.549151999719307
20-21	22.79226217483571	27.123232006028015	28.17040460820418	21.914101210932095
22-23	22.62639056349589	27.141606230031513	27.93915597614007	22.29284723033253
24-25	22.461301549034857	27.213470230737425	28.07754277861075	22.247685441616973
26-27	22.400387377999355	27.408650322776595	27.946836253902006	22.24412604532204
28-29	22.302175101454438	27.47408373634343	28.005356434145266	22.21838472805686
30-31	22.51556909155726	27.251301642071535	27.89253915929106	22.340590107080143
32-33	22.35800522848109	27.38157534488545	27.962537314751028	22.29788211188243
34-35	22.480863758029592	27.459319372848206	27.919314367503002	22.140502501619196
36-37	22.464317543484377	27.374940818891357	27.810585191952104	22.350156445672162
38-39	22.334862842387384	27.450347249782848	27.932550733595775	22.282239174233993
40-41	22.55688625341438	27.288905290496036	27.844824273529696	22.309384182559892
42-43	22.46948961619727	27.406766952953383	27.700464530232594	22.423278900616758
44-45	22.474297341443854	27.525156868259927	27.572059996056375	22.428485794239844
46-47	22.5855511072703	27.415389499303362	27.352145464806615	22.646913928619718
48-49	22.56486820673457	27.48063726225386	27.347132334141318	22.607362196870252
50-51	22.469060915435215	27.564579386658068	27.373393145806507	22.592966552100208
52-53	22.598909388499067	27.4742378206854	27.3179510797307	22.608901711084833
54-55	22.543735269966984	27.456689257690627	27.2986173072666	22.700958165075797
56-57	22.63404240216239	27.560073050922075	27.22357305683738	22.582311490078162
58-59	22.555978968256323	27.658235804323233	27.295888228797693	22.489896998622758
60-61	22.67325703934107	27.683075457324858	27.202825641643567	22.4408418616905
62-63	22.63236945323988	27.785325130072835	27.0584446774195	22.523860739267786
64-65	22.597715753136644	27.67050792533448	27.10158467451712	22.630191647011756
66-67	22.646419461576002	27.659827975538377	27.016903183292808	22.676849379592817
68-69	22.581973808897537	27.61387777539253	27.134250289700212	22.66989812600972
70-71	22.530865290023318	27.424344200228006	27.220431660709576	22.8243588490391
72-73	22.622603199453	27.45470149190952	27.178608383163176	22.744086925474306
74-75	22.529173549575727	27.54064355182267	27.185420507864077	22.744762390737527
76-77	22.63642756989149	27.50516699557086	27.054387394299233	22.804018040238407
78-79	22.53422186482636	27.473636000724987	27.113163588142307	22.878978546306346
80-81	22.565692892916307	27.541060194370765	27.14374875185649	22.749498160856447
82-83	22.60615157485398	27.470948568575455	27.28228154455246	22.640618312018105
84-85	22.69653152158867	27.49212287639407	27.13515131335276	22.6761942886645
86-87	22.538793960400536	27.53478504738476	27.238373051113147	22.68804794110156
88-89	22.598305764720404	27.440717195407977	27.19647265769658	22.76450438217504
90-91	22.62492693459304	27.560726692370626	27.169438286336028	22.64490808670031
92-93	22.66837816970416	27.463133681068975	27.235245879364612	22.63324226986225
94-95	22.445763791782053	27.535010686648153	27.2969112518589	22.722314269710893
96-97	22.4606499858671	27.695802838706385	27.37092243093982	22.472624744486698
98-99	23.625023398461742	29.64348582214587	27.50727772844506	19.224213050947327
100	30.409341327707924	41.14324260848593	28.44741606380615	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	742.0
1	680.0
2	895.5
3	1246.5
4	1634.5
5	2647.0
6	3596.0
7	4209.0
8	4467.0
9	4258.0
10	3849.0
11	3484.5
12	3801.5
13	5010.5
14	7729.5
15	11536.5
16	14834.0
17	17115.5
18	18240.0
19	18264.0
20	17688.0
21	17179.5
22	17327.0
23	18949.0
24	22568.0
25	28125.5
26	35871.0
27	45183.0
28	55493.0
29	67162.0
30	78210.0
31	88625.0
32	99588.0
33	110117.5
34	120150.5
35	130663.5
36	141199.5
37	151162.5
38	159872.5
39	165437.5
40	170933.0
41	178153.5
42	183932.5
43	188395.0
44	193214.5
45	198227.5
46	202335.0
47	205380.0
48	207223.0
49	205722.5
50	202504.0
51	198016.0
52	190662.5
53	181600.5
54	171444.5
55	160303.5
56	148332.5
57	134963.0
58	120278.0
59	103992.5
60	86875.0
61	71115.0
62	57285.5
63	44929.5
64	34256.0
65	26148.0
66	19718.5
67	14991.5
68	11458.0
69	8528.5
70	6390.5
71	4636.5
72	3345.0
73	2385.0
74	1637.5
75	1121.5
76	776.5
77	521.5
78	341.5
79	240.0
80	167.0
81	113.5
82	81.5
83	61.0
84	42.5
85	28.5
86	18.0
87	12.0
88	6.5
89	4.0
90	3.5
91	2.5
92	2.5
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.00833294226981453
3	0.002609526658178761
4	3.289319317032051E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	1.8639476129848293E-4
16-17	2.52181147639124E-4
18-19	0.0016227308630691454
20-21	7.675363694367456E-5
22-23	0.0016997086370420247
24-25	2.8517164481301296E-4
26-27	0.0027211400874736814
28-29	0.005830942848085051
30-31	0.003025703627378547
32-33	0.0022741609174185932
34-35	0.0023617929756506906
36-37	0.0028564564787055074
38-39	0.0
40-41	7.276075921304691E-4
42-43	0.00662569986107848
44-45	0.004064230636495676
46-47	6.231479718943804E-4
48-49	0.01624506266131258
50-51	0.01141280126243881
52-53	0.015710184933579747
54-55	0.013164776618963012
56-57	0.01136854918883596
58-59	0.0173338977670662
60-61	0.009227848600487634
62-63	0.02054226674740596
64-65	0.015221983997300846
66-67	0.03218226636555689
68-69	0.022362299607269667
70-71	0.02243106462896052
72-73	0.02007546776460356
74-75	0.01005365720631487
76-77	0.01281593000295502
78-79	0.013448333309915838
80-81	0.010514935134630437
82-83	0.004308156535392317
84-85	0.0031275561037175416
86-87	0.014077435920331735
88-89	0.001249830077114516
90-91	2.1974510196015771E-4
92-93	0.0012148753170265097
94-95	0.0030355165225122716
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	100.0
20-21	356.0
22-23	769.0
24-25	1547.0
26-27	2815.0
28-29	6790.0
30-31	14515.0
32-33	18486.0
34-35	24977.0
36-37	37855.0
38-39	43656.0
40-41	43044.0
42-43	46386.0
44-45	52967.0
46-47	52228.0
48-49	49008.0
50-51	45071.0
52-53	44106.0
54-55	47665.0
56-57	49426.0
58-59	48796.0
60-61	48022.0
62-63	46327.0
64-65	48546.0
66-67	51190.0
68-69	50026.0
70-71	47667.0
72-73	45858.0
74-75	46046.0
76-77	46234.0
78-79	49350.0
80-81	49489.0
82-83	47696.0
84-85	48196.0
86-87	51035.0
88-89	54249.0
90-91	56872.0
92-93	61809.0
94-95	75459.0
96-97	167820.0
98-99	938758.0
100-101	1899002.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.62847047722617
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.72998762969719	81.2988850051494
2	6.12160075970538	10.850962244098273
3	1.2814559863795898	3.4072045217012468
4	0.4458100874367981	1.5804586469136752
5	0.16941605403718213	0.7507542871801275
6	0.08912071868745568	0.47391797910602074
7	0.04860781836594992	0.30156256165062756
8	0.03071011520523063	0.2177432431055198
9	0.018568701216548265	0.14811440288141534
>10	0.06352184986364592	0.8472407465456139
>50	7.080873801582099E-4	0.045390610519657905
>100	4.92192024847841E-4	0.07776575114842363
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.1928795446880345E-5	0.0
3	0.0	0.0	0.0	2.1928795446880345E-5	0.0
4	0.0	0.0	0.0	2.1928795446880345E-5	0.0
5	0.0	2.1928795446880345E-5	0.0	2.1928795446880345E-5	0.0
6	2.1928795446880345E-5	2.1928795446880345E-5	0.0	2.1928795446880345E-5	0.0
7	2.1928795446880345E-5	2.1928795446880345E-5	0.0	2.1928795446880345E-5	0.0
8	2.1928795446880345E-5	2.1928795446880345E-5	0.0	2.1928795446880345E-5	0.0
9	2.1928795446880345E-5	8.771518178752138E-5	0.0	6.578638634064103E-5	0.0
10-11	2.1928795446880345E-5	9.867957951096155E-5	0.0	8.771518178752138E-5	0.0
12-13	2.1928795446880345E-5	1.0964397723440172E-4	0.0	8.771518178752138E-5	0.0
14-15	2.1928795446880345E-5	1.5350156812816241E-4	0.0	9.867957951096155E-5	1.0964397723440172E-5
16-17	4.385759089376069E-5	1.5350156812816241E-4	0.0	1.0964397723440172E-4	2.1928795446880345E-5
18-19	6.578638634064103E-5	1.7543036357504276E-4	0.0	1.0964397723440172E-4	2.1928795446880345E-5
20-21	6.578638634064103E-5	1.7543036357504276E-4	0.0	1.0964397723440172E-4	2.1928795446880345E-5
22-23	8.771518178752138E-5	2.1928795446880345E-4	0.0	1.3157277268128207E-4	2.1928795446880345E-5
24-25	1.206083749578419E-4	2.412167499156838E-4	0.0	1.8639476129848293E-4	2.1928795446880345E-5
26-27	1.3157277268128207E-4	2.412167499156838E-4	0.0	3.3989632942664537E-4	2.1928795446880345E-5
28-29	1.3157277268128207E-4	2.6314554536256414E-4	0.0	9.429382042158548E-4	2.1928795446880345E-5
30-31	1.5350156812816241E-4	2.6314554536256414E-4	0.0	0.002927494192158526	2.1928795446880345E-5
32-33	2.1928795446880345E-4	2.6314554536256414E-4	0.0	0.006118133929679616	2.1928795446880345E-5
34-35	2.3025235219224362E-4	2.960387385328847E-4	0.0	0.010317498257757202	2.1928795446880345E-5
36-37	2.412167499156838E-4	3.2893193170320517E-4	0.0	0.016391774596543056	2.1928795446880345E-5
38-39	2.412167499156838E-4	3.2893193170320517E-4	0.0	0.025053648798060792	2.1928795446880345E-5
40-41	2.412167499156838E-4	3.6182512487352566E-4	0.0	0.03361684342006757	2.1928795446880345E-5
42-43	2.412167499156838E-4	3.7278952259696586E-4	0.0	0.04260764955328851	2.1928795446880345E-5
44-45	2.412167499156838E-4	4.0568271576728635E-4	0.0	0.05158749128878601	2.1928795446880345E-5
46-47	2.412167499156838E-4	4.6050470438448724E-4	0.0	0.061422556046711846	2.1928795446880345E-5
48-49	2.412167499156838E-4	5.043622952782479E-4	0.0	0.0718606626794269	2.1928795446880345E-5
50-51	2.412167499156838E-4	5.262910907251283E-4	0.0	0.08165186984645896	2.1928795446880345E-5
52-53	2.850743408094445E-4	5.262910907251283E-4	0.0	0.09128957544536287	2.1928795446880345E-5
54-55	2.850743408094445E-4	5.262910907251283E-4	0.0	0.10072992188524485	2.1928795446880345E-5
56-57	2.850743408094445E-4	5.482198861720086E-4	0.0	0.11024701910919094	2.1928795446880345E-5
58-59	2.850743408094445E-4	5.482198861720086E-4	0.0	0.11910625246973058	2.1928795446880345E-5
60-61	2.850743408094445E-4	5.482198861720086E-4	0.0	0.12788873504620615	3.289319317032052E-5
62-63	2.850743408094445E-4	5.482198861720086E-4	0.0	0.1361120333387863	4.385759089376069E-5
64-65	2.850743408094445E-4	6.3593506795953E-4	0.0	0.14417086566551482	4.385759089376069E-5
66-67	2.850743408094445E-4	6.688282611298505E-4	0.0	0.15251477233305277	4.385759089376069E-5
68-69	3.0700313625632483E-4	6.797926588532906E-4	0.0	0.16038720989848282	4.385759089376069E-5
70-71	3.0700313625632483E-4	6.797926588532906E-4	0.0	0.16670270298718437	4.385759089376069E-5
72-73	3.0700313625632483E-4	6.797926588532906E-4	0.0	0.17165861075817934	4.385759089376069E-5
74-75	3.0700313625632483E-4	7.126858520236112E-4	0.0	0.1763952305747055	4.385759089376069E-5
76-77	3.0700313625632483E-4	7.675078406408121E-4	0.0	0.18050687972099555	4.385759089376069E-5
78-79	3.0700313625632483E-4	7.894366360876924E-4	0.0	0.18276554565202424	4.385759089376069E-5
80-81	3.0700313625632483E-4	7.894366360876924E-4	0.0	0.18362076867445257	4.385759089376069E-5
82-83	3.2893193170320517E-4	7.894366360876924E-4	0.0	0.18428959693558242	4.385759089376069E-5
84-85	3.508607271500855E-4	7.894366360876924E-4	0.0	0.18451984928777465	4.385759089376069E-5
86-87	3.508607271500855E-4	7.894366360876924E-4	0.0	0.1845856356741153	5.482198861720086E-5
88	3.7278952259696586E-4	8.332942269814531E-4	0.0	0.18459660007183873	6.578638634064103E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4450	0.0	42.84006	1
GTATCAA	7625	0.0	32.298386	1
TCAACGC	8445	0.0	27.899382	4
ATCAACG	8615	0.0	27.349442	3
CAACGCA	8660	0.0	27.111967	5
AACGCAG	9075	0.0	26.007355	6
TATCAAC	9335	0.0	25.768381	2
ACGCAGA	10700	0.0	22.09587	7
CGCAGAG	10810	0.0	21.833185	8
GTACATG	12555	0.0	21.635927	1
TACATGG	12640	0.0	20.84313	2
ACATGGG	12565	0.0	20.249262	3
TGCGCGA	420	1.4927307E-5	18.67331	94
CATGGGG	9535	0.0	18.532536	4
GCAGAGT	13690	0.0	17.29984	9
GTGGTAT	2700	0.0	16.3638	1
GAGTACT	6950	0.0	16.27363	12-13
TATAGCG	280	1.9257823E-7	16.069473	5
GAGTACA	9190	0.0	16.025482	1
CAGAGTA	13050	0.0	15.907157	10-11
>>END_MODULE