FastQCFastQC Report
Fri 27 May 2016
SRR2140314_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140314_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11622126
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC146700.044.950081
GGTATCA107950.041.6457441
TATACAC192000.037.4517483
TTATACA191850.035.1178052
GTATCAA188650.032.46091
TCAACGC207250.028.2615174
ATCAACG210050.027.8268453
CAACGCA211150.027.603925
TACACAT258850.027.289225
AACGCAG220950.026.4536276
TATCAAC227100.026.2805062
ACACATC260300.025.8801276
ACATCTC264850.025.0803178
ATACACA325800.023.2758544
CACATCT286250.023.148167
ACGCAGA264650.022.0082267
CGCAGAG268650.021.6196388
CATCTCC307650.021.5379859
GTACATG281400.020.8022881
TACATGG280650.020.099942
Produced by FastQC (version 0.11.5)