##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140314_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11622126
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.922630334587666	31.0	31.0	34.0	30.0	34.0
2	32.0341860860913	33.0	31.0	34.0	30.0	34.0
3	31.76764896543025	31.0	31.0	34.0	30.0	34.0
4	35.85034605544631	37.0	35.0	37.0	35.0	37.0
5	35.77812648047354	37.0	35.0	37.0	35.0	37.0
6	35.81946168885108	37.0	35.0	37.0	35.0	37.0
7	35.79945674311223	37.0	35.0	37.0	35.0	37.0
8	35.79347410275882	37.0	35.0	37.0	33.0	37.0
9	37.563305887408205	39.0	37.0	39.0	35.0	39.0
10-11	37.46396188614716	39.0	37.0	39.0	34.5	39.0
12-13	37.414291584861495	39.0	37.0	39.0	34.0	39.0
14-15	38.642913912652475	40.0	38.0	41.0	34.0	41.0
16-17	38.56800322075324	40.0	38.0	41.0	34.0	41.0
18-19	38.56364691795632	40.0	38.0	41.0	34.0	41.0
20-21	38.63196188869032	40.0	38.0	41.0	34.0	41.0
22-23	38.585290355161554	40.0	38.0	41.0	34.0	41.0
24-25	38.48637399959665	40.0	38.0	41.0	34.0	41.0
26-27	38.329793198669904	40.0	38.0	41.0	34.0	41.0
28-29	38.196982455421576	40.0	38.0	41.0	33.0	41.0
30-31	38.07766980052583	40.0	37.5	41.0	33.0	41.0
32-33	37.8756068837478	40.0	37.0	41.0	33.0	41.0
34-35	37.95969951551939	40.0	37.0	41.0	33.0	41.0
36-37	37.92721070452959	40.0	37.0	41.0	33.0	41.0
38-39	37.867461701340225	40.0	37.0	41.0	33.0	41.0
40-41	37.82525613942167	40.0	37.0	41.0	33.0	41.0
42-43	37.87263144699175	40.0	37.0	41.0	33.0	41.0
44-45	37.90927681360491	40.0	37.0	41.0	33.0	41.0
46-47	37.8600657567647	40.0	37.0	41.0	33.0	41.0
48-49	37.77389836652708	40.0	37.0	41.0	33.0	41.0
50-51	37.66144404305466	40.0	37.0	41.0	32.0	41.0
52-53	37.39709708739314	39.5	36.0	41.0	31.0	41.0
54-55	37.332002693481144	39.0	36.0	41.0	31.0	41.0
56-57	37.15999751087729	39.0	35.5	41.0	31.0	41.0
58-59	36.986652411271564	39.0	35.0	40.5	31.0	41.0
60-61	36.82130059665958	39.0	35.0	40.0	31.0	41.0
62-63	36.57389506313141	38.0	35.0	40.0	31.0	41.0
64-65	36.22562742685761	38.0	35.0	40.0	30.0	41.0
66-67	35.98492861807039	37.0	34.0	40.0	30.0	41.0
68-69	35.70489260483425	37.0	34.0	39.5	29.5	41.0
70-71	35.435514754375106	36.0	34.0	39.0	29.0	41.0
72-73	35.14281208881795	36.0	34.0	39.0	29.0	40.0
74-75	34.8171651294446	35.5	34.0	38.5	29.0	40.0
76-77	33.57597475350664	34.5	31.5	36.5	28.0	39.0
78-79	34.21482777819138	35.0	33.0	37.0	29.0	39.0
80-81	34.15146149204654	35.0	33.5	36.5	29.0	39.0
82-83	33.88204878218865	35.0	33.0	36.0	29.0	38.0
84-85	33.648446089558334	35.0	33.0	36.0	29.0	37.0
86-87	33.44697815350585	35.0	33.0	35.0	29.0	37.0
88-89	33.27765074699229	35.0	33.0	35.0	29.0	36.0
90-91	33.14608394767602	35.0	33.0	35.0	29.0	36.0
92-93	33.124532555775794	35.0	33.0	35.0	29.0	36.0
94-95	33.12250184505849	35.0	33.0	35.0	29.0	35.5
96-97	33.11624605035976	35.0	33.0	35.0	29.0	35.0
98-99	33.23083356254089	34.0	33.0	35.0	29.0	35.0
100	32.08634441262045	34.0	31.0	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	14.0
20	84.0
21	444.0
22	2021.0
23	6890.0
24	17857.0
25	38165.0
26	68643.0
27	107277.0
28	154465.0
29	204676.0
30	259228.0
31	320959.0
32	395820.0
33	499397.0
34	659459.0
35	902864.0
36	1372906.0
37	2285580.0
38	2814550.0
39	1460314.0
40	50510.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.84639935929106	20.63522629164406	13.645618710380528	24.872755638684353
2	14.810555982171838	21.34280180539574	40.63316721113307	23.21347500129935
3	17.926875543696646	26.032647182070296	31.073798188409224	24.966679085823834
4	12.567383609659444	18.272353556081242	38.031739077275084	31.12852375698423
5	12.979518549360074	37.848144134730596	36.09170989886016	13.08062741704917
6	30.13688717537566	37.92363806759624	18.441169885785097	13.498304871243006
7	26.45588251237338	32.3792910178396	22.78041900423382	18.384407465553203
8	24.60641022133128	36.65963525089988	21.326674654878115	17.407279872890726
9	25.09563224490941	16.42492087936407	20.899067864175624	37.580379011550896
10-11	24.136423060634517	26.552009503252677	29.03276904759078	20.27879838852203
12-13	24.80631340599818	23.88017906534484	28.993636792442278	22.3198707362147
14-15	21.9456655484389	25.56418216811826	26.02559578142025	26.46455650202259
16-17	22.02667608780311	28.93640337102763	26.7483381222007	22.28858241896856
18-19	22.071691015849236	27.959854986051567	28.2315786729323	21.736875325166896
20-21	22.48305426378601	27.089265685481113	28.080152955839765	22.34752709489311
22-23	22.26658314642682	27.44664806192798	27.958608257230406	22.328160534414792
24-25	22.3288627021347	27.544688523111365	28.075329229038193	22.051119545715743
26-27	22.23683590726226	27.59508949181319	28.097397239830553	22.070677361093995
28-29	21.889904642607032	27.36127977815055	28.400065725381413	22.348749853861
30-31	22.232728925445155	27.203371362380185	27.937448584116215	22.62645112805845
32-33	22.35319159348306	27.293394214472848	28.332821226721617	22.02059296532248
34-35	22.30929524253488	27.735816401609302	28.189476732758518	21.7654116230973
36-37	22.0933967551765	27.498424901357026	27.94604468595204	22.462133657514435
38-39	22.082391218237525	27.343530966510844	28.235438642613214	22.33863917263842
40-41	22.21496912030439	27.23428183963356	28.320386368346938	22.23036267171511
42-43	22.40905145975986	27.192742748025978	28.217254519301516	22.180951272912647
44-45	22.103119098022937	27.561512255767788	28.073203690728533	22.26216495548074
46-47	22.561923307789478	27.153063505259507	27.598973345153084	22.686039841797935
48-49	22.330172042392014	27.28446733399574	27.64594873890534	22.739411884706904
50-51	22.11936396377992	27.40549202520908	27.945771177425154	22.529372833585846
52-53	22.214335620363475	27.503377468500183	28.11236386809194	22.169923043044403
54-55	22.188370358273122	27.525539465005465	27.71734572643233	22.56874445028908
56-57	22.564428747173586	27.836336676844258	27.25889927524654	22.34033530073562
58-59	22.008998256794907	28.226560894422025	27.808957862158884	21.955482986624183
60-61	22.515705412988915	28.495933417848974	27.038798577701574	21.949562591460538
62-63	22.176932174958914	28.743049379640016	27.033889382962133	22.046129062438936
64-65	22.171933621575015	28.47549823607017	27.133923648219575	22.21864449413524
66-67	22.295843607083548	28.214039132380947	27.189447624255674	22.300669636279828
68-69	22.311016063553275	27.910259185783037	27.362414578455162	22.416310172208522
70-71	22.325414472819542	27.672262709113976	27.448767913996726	22.55355490406976
72-73	22.3804563448412	27.667162190600386	27.405884946969376	22.546496517589034
74-75	22.270177047872345	27.798339096525954	27.41351719836595	22.517966657235753
76-77	22.41334187631117	27.72637968043555	27.319304077252898	22.540974366000388
78-79	22.32254880951038	27.710368707774556	27.324229368042317	22.642853114672743
80-81	22.320242502997957	27.75358716835395	27.39080235408763	22.535367974560465
82-83	22.33369720548967	27.75659248684007	27.504688129789884	22.405022177880376
84-85	22.46613520474899	27.73590544817247	27.379018694286415	22.418940652792127
86-87	22.30790144317431	27.763425777578842	27.428388257719007	22.500284521527842
88-89	22.400501126608066	27.70955494604778	27.351567562289336	22.538376365054816
90-91	22.37243328993265	27.799996944539444	27.387823940420493	22.43974582510741
92-93	22.411083043026476	27.721854203266073	27.49025068309833	22.37681207060912
94-95	22.242626184670023	27.84854156892822	27.487975969552036	22.42085627684972
96-97	22.267914627810928	27.93329012815244	27.570481336001425	22.228313908035208
98-99	23.388527250095137	29.914995689740714	27.733031042992934	18.963446017171215
100	29.957749005586038	41.37603385028425	28.666217144129707	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1877.0
1	1687.0
2	2346.0
3	3404.5
4	4407.5
5	6884.5
6	9186.0
7	10714.5
8	11741.0
9	11672.0
10	10776.5
11	9614.0
12	9917.0
13	12817.5
14	19836.5
15	29688.0
16	38426.5
17	44503.5
18	47374.0
19	47268.5
20	45912.5
21	44746.5
22	45845.0
23	51137.5
24	61284.5
25	76734.0
26	97226.5
27	121211.0
28	147369.0
29	176886.5
30	205604.0
31	232741.5
32	260296.5
33	285857.5
34	309940.5
35	334272.0
36	361688.5
37	388096.5
38	407826.5
39	422307.0
40	438761.5
41	457047.5
42	470381.5
43	482189.0
44	493829.5
45	502896.0
46	519303.5
47	539148.0
48	560101.5
49	559407.5
50	535915.5
51	515394.5
52	488855.0
53	455006.0
54	419230.5
55	386143.0
56	354119.5
57	320357.0
58	283391.0
59	240944.0
60	198372.0
61	161020.0
62	129008.0
63	101358.5
64	77243.0
65	59021.0
66	45344.5
67	35118.5
68	27131.0
69	20288.0
70	15151.0
71	11315.0
72	8324.5
73	5962.5
74	4165.0
75	2935.0
76	2009.0
77	1334.5
78	899.5
79	581.0
80	381.5
81	263.0
82	194.0
83	130.0
84	82.0
85	60.0
86	46.5
87	35.0
88	23.5
89	14.5
90	10.0
91	6.5
92	4.5
93	3.5
94	2.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.008277315183125704
3	0.002546866210192524
4	4.216096091197084E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	1.3336630492562205E-4
16-17	2.75336887588381E-4
18-19	0.001802596185930182
20-21	6.883696421071579E-5
22-23	0.0016780643089318115
24-25	2.8835109353709225E-4
26-27	0.002609210979766473
28-29	0.005614697321276601
30-31	0.0028621121152774148
32-33	0.0022302665796501814
34-35	0.002339454093170629
36-37	0.0024670796867176234
38-39	0.0
40-41	7.033441342657243E-4
42-43	0.006340332893089855
44-45	0.0038280708484639764
46-47	5.926684432630689E-4
48-49	0.01585189358792531
50-51	0.011223428392342305
52-53	0.01472922808591806
54-55	0.012947249146088384
56-57	0.011233268728283783
58-59	0.017425571445900068
60-61	0.00900852017666864
62-63	0.020011517987283747
64-65	0.014596297858335269
66-67	0.03023293201618578
68-69	0.0214910983014153
70-71	0.020986788835199964
72-73	0.019269679159841988
74-75	0.00959859616407992
76-77	0.012068125458851784
78-79	0.01230378368978406
80-81	0.009461515158462084
82-83	0.0044699313630733924
84-85	0.0029510420940876874
86-87	0.01386626467818666
88-89	0.0012175469228046176
90-91	2.2176680628940517E-4
92-93	0.0010413549659144445
94-95	0.0028307988610453707
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	291.0
20-21	910.0
22-23	2033.0
24-25	4747.0
26-27	7333.0
28-29	16314.0
30-31	34785.0
32-33	44052.0
34-35	60216.0
36-37	89771.0
38-39	104544.0
40-41	103240.0
42-43	112238.0
44-45	126616.0
46-47	125865.0
48-49	118472.0
50-51	110694.0
52-53	107922.0
54-55	119672.0
56-57	130106.0
58-59	132972.0
60-61	136405.0
62-63	138122.0
64-65	146484.0
66-67	156112.0
68-69	139057.0
70-71	119091.0
72-73	111255.0
74-75	112864.0
76-77	113002.0
78-79	120146.0
80-81	122389.0
82-83	119409.0
84-85	120032.0
86-87	127234.0
88-89	135499.0
90-91	143937.0
92-93	156533.0
94-95	194762.0
96-97	430367.0
98-99	2377043.0
100-101	4849590.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.33850621583157
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.33045599839231	75.33997218483479
2	7.266861860324222	12.257525483530877
3	1.8655365553697647	4.720096991127417
4	0.7168213881651969	2.4182258040564593
5	0.333065488621541	1.404512289119341
6	0.1714351496187433	0.8675150659039437
7	0.09807933101242297	0.5790304987765086
8	0.05932596464328302	0.4002770590262184
9	0.037302063610047016	0.28314002912753655
>10	0.11826028863985212	1.518351425875083
>50	0.0023019006563701223	0.12893919241719162
>100	5.370291129111604E-4	0.0710707743719856
>500	1.6981833303379518E-5	0.01134320183261725
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.604277737136906E-6
2	0.0	8.604277737136906E-6	0.0	8.604277737136906E-6	8.604277737136906E-6
3	0.0	8.604277737136906E-6	0.0	8.604277737136906E-6	8.604277737136906E-6
4	0.0	3.4417110948547624E-5	0.0	8.604277737136906E-6	8.604277737136906E-6
5	0.0	3.4417110948547624E-5	0.0	8.604277737136906E-6	8.604277737136906E-6
6	0.0	3.4417110948547624E-5	0.0	8.604277737136906E-6	2.5812833211410718E-5
7	0.0	3.4417110948547624E-5	0.0	2.5812833211410718E-5	2.5812833211410718E-5
8	0.0	3.4417110948547624E-5	0.0	2.5812833211410718E-5	2.5812833211410718E-5
9	0.0	1.1185561058277978E-4	0.0	3.4417110948547624E-5	2.5812833211410718E-5
10-11	0.0	1.1615774945134824E-4	0.0	4.302138868568453E-5	2.5812833211410718E-5
12-13	0.0	1.2045988831991668E-4	0.0	4.302138868568453E-5	3.011497207997917E-5
14-15	0.0	1.8499197134844348E-4	0.0	5.1625666422821435E-5	4.732352755425298E-5
16-17	0.0	1.9789838795414885E-4	0.0	6.022994415995834E-5	6.022994415995834E-5
18-19	0.0	2.0650266569128574E-4	0.0	6.883422189709525E-5	6.022994415995834E-5
20-21	0.0	2.194090822969911E-4	0.0	9.464705510850596E-5	6.022994415995834E-5
22-23	0.0	2.581283321141072E-4	0.0	1.290641660570536E-4	6.45320830285268E-5
24-25	0.0	2.925454430626548E-4	0.0	1.763876936113066E-4	6.883422189709525E-5
26-27	0.0	3.183582762740655E-4	0.0	2.28013360034128E-4	7.743849963423215E-5
28-29	0.0	3.183582762740655E-4	0.0	5.678823306510359E-4	7.743849963423215E-5
30-31	0.0	3.7858822043402386E-4	0.0	0.0017896897693244766	7.743849963423215E-5
32-33	0.0	3.8719249817116075E-4	0.0	0.004401088062545527	7.743849963423215E-5
34-35	0.0	4.517245811996876E-4	0.0	0.007752454241160353	7.743849963423215E-5
36-37	0.0	5.162566642282144E-4	0.0	0.012398764219214282	7.743849963423215E-5
38-39	0.0	5.291630808339197E-4	0.0	0.0196650767682264	7.743849963423215E-5
40-41	0.0	5.850908861253097E-4	0.0	0.027249747593512584	7.743849963423215E-5
42-43	0.0	6.022994415995834E-4	0.0	0.03467523928066173	7.743849963423215E-5
44-45	0.0	6.797379412338156E-4	0.0	0.04224270155047364	7.743849963423215E-5
46-47	0.0	7.485721631309108E-4	0.0	0.0507437279547649	8.604277737136906E-5
48-49	0.0	7.485721631309108E-4	0.0	0.059352307830770376	1.0325133284564287E-4
50-51	0.0	8.088021072908692E-4	0.0	0.06731126473762201	1.0325133284564287E-4
52-53	0.0	8.174063850280061E-4	0.0	0.0755369542543249	1.0755347171421132E-4
54-55	0.0	8.518234959765537E-4	0.0	0.08337545127285662	1.1185561058277978E-4
56-57	0.0	8.776363291879644E-4	0.0	0.09111930123627983	1.1185561058277978E-4
58-59	0.0	8.819384680565328E-4	0.0	0.09914709236502856	1.1185561058277978E-4
60-61	0.0	9.464705510850597E-4	0.0	0.10648654127480635	1.1185561058277978E-4
62-63	0.0	9.679812454279019E-4	0.0	0.11372704099060706	1.2045988831991668E-4
64-65	0.0	0.0010411176061935657	0.0	0.12080836156827073	1.2045988831991668E-4
66-67	0.0	0.0010411176061935657	0.0	0.12819943614447132	1.2045988831991668E-4
68-69	0.0	0.0010497218839307025	0.0	0.13489356422396384	1.2045988831991668E-4
70-71	0.0	0.0010669304394049764	0.0	0.14073156666861125	1.290641660570536E-4
72-73	0.0	0.0010798368560106816	0.0	0.1451111440368139	1.290641660570536E-4
74-75	0.0	0.0011099518280906608	0.0	0.1490820182125026	1.290641660570536E-4
76-77	0.0	0.001148671077907777	0.0	0.15286359827797427	1.290641660570536E-4
78-79	0.0	0.0011615774945134823	0.0	0.15514803401718413	1.290641660570536E-4
80-81	0.0	0.0011701817722506193	0.0	0.1559009083191836	1.376684437941905E-4
82-83	0.0	0.0011787860499877561	0.0	0.1563913521502004	1.548769992684643E-4
84-85	0.0	0.0011787860499877561	0.0	0.15659785481589167	1.548769992684643E-4
86-87	0.0	0.0011959946054620298	0.0	0.1567183147042116	1.548769992684643E-4
88	0.0	0.0012218074386734406	0.0	0.1567183147042116	1.548769992684643E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	14670	0.0	44.95008	1
GGTATCA	10795	0.0	41.645744	1
TATACAC	19200	0.0	37.451748	3
TTATACA	19185	0.0	35.117805	2
GTATCAA	18865	0.0	32.4609	1
TCAACGC	20725	0.0	28.261517	4
ATCAACG	21005	0.0	27.826845	3
CAACGCA	21115	0.0	27.60392	5
TACACAT	25885	0.0	27.28922	5
AACGCAG	22095	0.0	26.453627	6
TATCAAC	22710	0.0	26.280506	2
ACACATC	26030	0.0	25.880127	6
ACATCTC	26485	0.0	25.080317	8
ATACACA	32580	0.0	23.275854	4
CACATCT	28625	0.0	23.14816	7
ACGCAGA	26465	0.0	22.008226	7
CGCAGAG	26865	0.0	21.619638	8
CATCTCC	30765	0.0	21.537985	9
GTACATG	28140	0.0	20.802288	1
TACATGG	28065	0.0	20.09994	2
>>END_MODULE