FastQCFastQC Report
Fri 27 May 2016
SRR2140313_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140313_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11440514
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102650.040.496541
GTATCAA180700.032.4641081
TCAACGC202400.028.0125054
ATCAACG202350.027.9797233
CAACGCA206000.027.5625695
AACGCAG217700.026.099996
TATCAAC221000.026.0457862
ACGCAGA252500.022.4220737
CGCAGAG257500.022.0027268
GTACATG286600.021.4221481
TACATGG289350.020.781492
ACATGGG292000.019.9900533
CTTATAC82750.018.3390851
CATGGGG219650.017.8459574
GCAGAGT316800.017.7296529
CGTCGAA5255.0424096E-616.92278994
AGAGTAC278000.016.62411310-11
GAGTACT166400.016.2403512-13
TATACAC119200.016.1518733
CAGAGTA308550.015.52675910-11
Produced by FastQC (version 0.11.5)