FastQCFastQC Report
Fri 27 May 2016
SRR2140312_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140312_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8468275
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79000.043.0991021
GTATCAA133350.031.6327531
TCAACGC151750.026.9046974
ATCAACG151600.026.8510783
CAACGCA154100.026.4413645
TATCAAC162100.025.4172082
AACGCAG163050.025.1152946
CGTCGAA4101.7915227E-824.06117294
GTACATG225200.022.923731
TACATGG225100.022.4073242
ACGCAGA191800.021.3506187
ACATGGG230550.021.2901883
CGCAGAG196900.020.8183618
CATGGGG174050.018.8552764
TGCGCGA4653.394634E-516.97218194
GCAGAGT240050.016.9569979
GAGTACT126600.016.56129312-13
ATGGGGG110400.016.1772125
AGAGTAC214950.015.59105110-11
CAGAGTA234950.015.57719110-11
Produced by FastQC (version 0.11.5)