FastQCFastQC Report
Fri 27 May 2016
SRR2140311_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140311_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7831368
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69300.039.408841
GTATCAA118750.031.3954161
ATCAACG129300.027.7608573
TCAACGC130550.027.43134
CAACGCA133050.027.1603855
AACGCAG137450.026.3205436
TATCAAC142950.025.852712
ACGCAGA166150.021.7250657
GTACATG195100.021.1953621
CGCAGAG170700.021.1221458
TACATGG197650.020.3659112
ACATGGG200000.019.493883
GCAGAGT210750.017.1468079
CATGGGG157700.016.7734454
GAGTACT109050.016.17732812-13
AGAGTAC182500.015.76501410-11
GAGTACA142150.015.1178991
CAGAGTA206850.015.06008710-11
TATACCG5500.014.7980725
GTGGTAT44050.014.7835411
Produced by FastQC (version 0.11.5)