##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140311_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7831368
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.00469049596443	31.0	31.0	34.0	30.0	34.0
2	32.10493415709746	33.0	31.0	34.0	30.0	34.0
3	31.84622367382046	31.0	31.0	34.0	30.0	34.0
4	35.90514722332037	37.0	35.0	37.0	35.0	37.0
5	35.841550160840356	37.0	35.0	37.0	35.0	37.0
6	35.88293003214764	37.0	35.0	37.0	35.0	37.0
7	35.86578717792345	37.0	35.0	37.0	35.0	37.0
8	35.86353648557953	37.0	35.0	37.0	35.0	37.0
9	37.64309773209483	39.0	37.0	39.0	35.0	39.0
10-11	37.550055175545324	39.0	37.0	39.0	35.0	39.0
12-13	37.506865339491135	39.0	37.0	39.0	35.0	39.0
14-15	38.77001342549603	40.0	38.0	41.0	34.5	41.0
16-17	38.70822026241137	40.0	38.0	41.0	34.0	41.0
18-19	38.69937091450689	40.0	38.0	41.0	34.0	41.0
20-21	38.760177299667916	40.0	38.0	41.0	34.0	41.0
22-23	38.71700624247124	40.0	38.0	41.0	34.0	41.0
24-25	38.6272979995727	40.0	38.0	41.0	34.0	41.0
26-27	38.483303603382694	40.0	38.0	41.0	34.0	41.0
28-29	38.35807413966752	40.0	38.0	41.0	34.0	41.0
30-31	38.24380948576268	40.0	38.0	41.0	34.0	41.0
32-33	38.065908530060675	40.0	37.5	41.0	33.0	41.0
34-35	38.15870558022403	40.0	38.0	41.0	33.0	41.0
36-37	38.11981215966231	40.0	38.0	41.0	33.0	41.0
38-39	38.086484714465755	40.0	38.0	41.0	33.0	41.0
40-41	38.02700148943974	40.0	37.0	41.0	33.0	41.0
42-43	38.059029493216926	40.0	37.0	41.0	33.0	41.0
44-45	38.08720329955182	40.0	37.0	41.0	33.0	41.0
46-47	38.031928547326125	40.0	37.0	41.0	33.0	41.0
48-49	37.941473174103336	40.0	37.0	41.0	33.0	41.0
50-51	37.84367525002227	40.0	37.0	41.0	33.0	41.0
52-53	37.57020721305945	40.0	36.0	41.0	32.0	41.0
54-55	37.514019439403874	39.0	36.0	41.0	32.0	41.0
56-57	37.34521273693381	39.0	36.0	41.0	31.5	41.0
58-59	37.16922577526152	39.0	35.0	41.0	31.0	41.0
60-61	37.0153775287268	39.0	35.0	40.5	31.0	41.0
62-63	36.76477080732364	38.5	35.0	40.0	31.0	41.0
64-65	36.396728733294154	38.0	35.0	40.0	31.0	41.0
66-67	36.14401863364972	37.0	35.0	40.0	30.0	41.0
68-69	35.84912208972537	37.0	34.0	39.5	30.0	41.0
70-71	35.56914150075586	36.0	34.0	39.0	30.0	41.0
72-73	35.27117684756756	36.0	34.0	39.0	30.0	40.5
74-75	34.945719652876235	35.5	34.0	38.5	29.5	40.0
76-77	33.69869502883361	34.5	32.5	36.5	28.5	39.0
78-79	34.314184556223935	35.0	33.5	37.0	29.0	39.0
80-81	34.24172126700193	35.0	34.0	36.5	29.0	39.0
82-83	33.97418065712968	35.0	34.0	36.0	29.0	37.5
84-85	33.74675314713908	35.0	33.0	36.0	29.0	37.0
86-87	33.55346430398076	35.0	33.0	35.0	29.0	37.0
88-89	33.390832429478834	35.0	33.0	35.0	29.0	36.0
90-91	33.263187802760086	35.0	33.0	35.0	29.0	36.0
92-93	33.2439753910554	35.0	33.0	35.0	29.0	36.0
94-95	33.2402192402667	35.0	33.0	35.0	29.0	35.5
96-97	33.225586789410556	35.0	33.0	35.0	29.0	35.0
98-99	33.327959751823116	35.0	33.0	35.0	29.5	35.0
100	32.19573892271363	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	11.0
20	48.0
21	256.0
22	1160.0
23	3992.0
24	10506.0
25	22908.0
26	41165.0
27	65106.0
28	93755.0
29	125735.0
30	159872.0
31	199894.0
32	247700.0
33	312960.0
34	419389.0
35	581657.0
36	901135.0
37	1536277.0
38	1957106.0
39	1107891.0
40	42845.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.1247986303287	20.035669374750363	13.216694707744548	24.62283728717639
2	15.260766104429035	21.22144616328431	40.11294471246246	23.404843019824195
3	18.455800224156466	25.51005045627474	30.341839250162522	25.692310069406272
4	12.792752483394557	17.47368929134142	38.391575771993395	31.341982453270635
5	13.337222819818964	37.761384728696186	35.726669976433236	13.174722475051611
6	30.984905829990367	37.51026640556286	18.363560491602488	13.141267272844285
7	27.2980914700982	32.10634463863784	22.853542318532345	17.74202157273161
8	24.939269869580897	36.49827207711348	20.973551491897712	17.588906561407917
9	25.70802444732517	16.290053027772412	20.92225777156686	37.079664753335564
10-11	24.59429821201098	26.385639137376764	28.897084136513566	20.122978514098687
12-13	25.12630615749381	23.775258677666532	28.992769845574873	22.105665319264784
14-15	22.16716575028606	25.415799798578863	26.21112213943108	26.205912311703994
16-17	22.17641910958979	28.519141442552883	26.94893233863836	22.355507109218973
18-19	22.144771638682556	27.985220712455135	28.281240622482034	21.588767026380275
20-21	22.810143199004987	27.21417662487581	28.107765743079483	21.86791443303972
22-23	22.641598831497586	27.09990500234764	27.908103420432713	22.350392745722054
24-25	22.44632205064351	27.161240683083733	28.00501385701988	22.387423409252886
26-27	22.452734651160785	27.347751124270147	27.86829051837024	22.331223706198827
28-29	22.415045219926988	27.467829244634455	27.853771520254472	22.263354015184085
30-31	22.622067367579067	27.293218422981063	27.801625418453213	22.283088790986664
32-33	22.37902508945179	27.40375666335386	27.82036356046963	22.39685468672472
34-35	22.535268306007154	27.42302984519258	27.795837204094653	22.24586464470561
36-37	22.55457122310886	27.379052813474214	27.687036763617378	22.37933919979955
38-39	22.390468406486754	27.498288145830745	27.803005735671338	22.308237712011163
40-41	22.596293243137428	27.353425407400927	27.705902076317717	22.344379273143925
42-43	22.523204160097734	27.411407428855057	27.554591334322193	22.510797076725016
44-45	22.582292730580654	27.4909682511802	27.45775651576165	22.4689825024775
46-47	22.572200723498195	27.46915154048593	27.30230128042751	22.65634645558837
48-49	22.5683386647062	27.52953174686244	27.305066423582197	22.597063164849164
50-51	22.50654812556675	27.579267995485502	27.312053973673585	22.602129905274165
52-53	22.63090165969847	27.53395710762756	27.186826492380316	22.64831474029365
54-55	22.626767517785353	27.419428530313795	27.240292480236	22.71351147166485
56-57	22.641690439230146	27.5062915376424	27.23579311470612	22.616224908421334
58-59	22.58932669290289	27.600723978668995	27.267086761980835	22.542862566447276
60-61	22.70249093469687	27.51315110512016	27.22481406388479	22.559543896298177
62-63	22.70004211667772	27.605999690176162	27.10106852915975	22.59288966398637
64-65	22.59414543108207	27.528291503808553	27.170310576574174	22.7072524885352
66-67	22.6468376210545	27.54135516494818	27.07547774372234	22.736329470274974
68-69	22.585851425047046	27.52167785782137	27.190837880784223	22.701632836347358
70-71	22.578644292865597	27.331097409369626	27.199661877150984	22.89059642061379
72-73	22.67571376806764	27.374106691647587	27.17257075268765	22.777608787597117
74-75	22.530161358510394	27.521859541523614	27.13856401689257	22.80941508307342
76-77	22.65932310631209	27.431022200436317	27.06317766239878	22.84647703085281
78-79	22.593014893439246	27.427890260604688	27.081249233779197	22.89784561217687
80-81	22.566075984820795	27.506421444893412	27.109709361305327	22.817793208980458
82-83	22.626051334175852	27.415037430555998	27.215365620672614	22.743545614595533
84-85	22.707115137472826	27.419991774814257	27.140553578219652	22.732339509493265
86-87	22.56059987418674	27.44483881649804	27.208287028553034	22.786274280762182
88-89	22.647412655220126	27.38563361745896	27.138882668348767	22.828071058972142
90-91	22.63730166223735	27.454179503008387	27.20212126917691	22.70639756557736
92-93	22.689994002386808	27.451003096334887	27.154087823847345	22.704915077430964
94-95	22.528378379937447	27.49962792854844	27.23561102592886	22.736382665585257
96-97	22.52654263488226	27.627021171044923	27.325249457653406	22.52118673641941
98-99	23.700543453198726	29.579861343737196	27.487085808354006	19.232509394710075
100	30.517059765860317	41.00669552542101	28.476244708718674	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	978.0
1	882.5
2	1263.5
3	1828.5
4	2316.5
5	3762.0
6	5162.0
7	6071.0
8	6540.5
9	6332.0
10	5775.5
11	5214.5
12	5614.0
13	7299.5
14	11083.0
15	16470.0
16	21395.5
17	24934.5
18	26737.5
19	27125.5
20	26878.0
21	26790.0
22	27858.5
23	31032.5
24	37046.0
25	46519.5
26	59583.0
27	75089.0
28	92281.0
29	112334.5
30	132244.5
31	150929.5
32	170828.5
33	189826.0
34	208674.0
35	228169.5
36	247241.5
37	264371.0
38	278783.5
39	290779.5
40	303122.0
41	315254.0
42	323700.5
43	331685.0
44	341470.5
45	350179.5
46	354554.0
47	357994.5
48	359338.0
49	354345.5
50	349109.5
51	341961.5
52	327965.0
53	311312.0
54	293345.5
55	273804.5
56	253758.5
57	231578.5
58	206401.0
59	177547.5
60	148560.0
61	122349.5
62	99447.0
63	78859.0
64	59942.5
65	45635.0
66	34651.5
67	26809.5
68	20664.5
69	15538.0
70	11784.0
71	8769.5
72	6574.0
73	4751.5
74	3276.5
75	2231.0
76	1518.5
77	1029.0
78	682.0
79	450.5
80	301.5
81	204.0
82	144.0
83	96.0
84	65.5
85	45.0
86	34.0
87	24.5
88	17.0
89	10.5
90	6.5
91	6.0
92	6.0
93	3.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.008095648167727528
3	0.002949676225149935
4	6.512272185395961E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	1.9153741721752827E-4
16-17	2.873061258262924E-4
18-19	0.00176214423840126
20-21	5.107862737426229E-5
22-23	0.001596359075304536
24-25	2.554694900132825E-4
26-27	0.0025236939723965383
28-29	0.005351940951402458
30-31	0.0028255425458151152
32-33	0.00213461952303727
34-35	0.002376816480380446
36-37	0.0027370111915802514
38-39	0.0
40-41	6.099895738195126E-4
42-43	0.005828041817472831
44-45	0.004011540225448561
46-47	4.872618764935263E-4
48-49	0.01614927601350257
50-51	0.01095659283437422
52-53	0.01531945654192357
54-55	0.01281280584189127
56-57	0.011252808285795984
58-59	0.017481855935787154
60-61	0.009330207626626754
62-63	0.02035073905096586
64-65	0.014943946801999211
66-67	0.03157401710457034
68-69	0.022278534349152744
70-71	0.022116262522187992
72-73	0.02130841933773788
74-75	0.010713250400171412
76-77	0.01275905527906327
78-79	0.013233831967519987
80-81	0.009861751899667988
82-83	0.004948433595444322
84-85	0.003429974778252131
86-87	0.014295729077271854
88-89	0.0012889741064164315
90-91	2.679202872475025E-4
92-93	0.0012520366777084378
94-95	0.0027688271594256066
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	187.0
20-21	611.0
22-23	1316.0
24-25	2556.0
26-27	4745.0
28-29	11898.0
30-31	26104.0
32-33	32669.0
34-35	44658.0
36-37	68468.0
38-39	78428.0
40-41	76110.0
42-43	82335.0
44-45	92096.0
46-47	91631.0
48-49	87127.0
50-51	80843.0
52-53	78770.0
54-55	84199.0
56-57	86671.0
58-59	85602.0
60-61	82744.0
62-63	80123.0
64-65	83243.0
66-67	86286.0
68-69	86750.0
70-71	84913.0
72-73	80634.0
74-75	82227.0
76-77	82715.0
78-79	86807.0
80-81	87190.0
82-83	84733.0
84-85	84357.0
86-87	89729.0
88-89	94904.0
90-91	99738.0
92-93	106377.0
94-95	131119.0
96-97	286839.0
98-99	1597485.0
100-101	3215431.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.25776881719591
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.06396979993877	76.78653115962094
2	6.8439194191376345	11.669945992807083
3	1.7003776193242452	4.349112059108414
4	0.6412523097233963	2.1868696470356093
5	0.29537496414441367	1.2591505203705975
6	0.150632351245634	0.7705546907334551
7	0.08444127017676296	0.5039492003952585
8	0.0552503499947026	0.3768417253534012
9	0.03668999996628377	0.28152967815255125
>10	0.12562419745506156	1.6348357719791606
>50	0.0020511842257421938	0.11819662791975026
>100	3.919417071607753E-4	0.05149600413623488
>500	2.4592960181222747E-5	0.010986922387584652
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.2769161147835218E-5	0.0	0.0	0.0
3	0.0	1.2769161147835218E-5	0.0	0.0	0.0
4	0.0	1.2769161147835218E-5	0.0	0.0	0.0
5	0.0	1.2769161147835218E-5	0.0	1.2769161147835218E-5	0.0
6	0.0	1.2769161147835218E-5	0.0	1.2769161147835218E-5	0.0
7	0.0	1.2769161147835218E-5	0.0	2.5538322295670436E-5	0.0
8	0.0	1.2769161147835218E-5	0.0	2.5538322295670436E-5	0.0
9	0.0	1.2769161147835218E-5	0.0	3.830748344350566E-5	0.0
10-11	0.0	1.2769161147835218E-5	0.0	6.384580573917609E-5	0.0
12-13	0.0	1.2769161147835218E-5	0.0	7.02303863130937E-5	6.384580573917609E-6
14-15	0.0	3.192290286958805E-5	0.0	8.299954746092892E-5	1.915374172175283E-5
16-17	0.0	3.830748344350566E-5	0.0	8.938412803484653E-5	2.5538322295670436E-5
18-19	0.0	3.830748344350566E-5	0.0	9.576870860876414E-5	2.5538322295670436E-5
20-21	0.0	3.830748344350566E-5	0.0	1.2769161147835217E-4	2.5538322295670436E-5
22-23	0.0	3.830748344350566E-5	0.0	1.5322993377402263E-4	5.107664459134087E-5
24-25	0.0	5.107664459134087E-5	0.0	2.1707573951319873E-4	6.384580573917609E-5
26-27	0.0	5.107664459134087E-5	0.0	3.128444481219629E-4	6.384580573917609E-5
28-29	0.0	5.107664459134087E-5	0.0	8.8107211920063E-4	6.384580573917609E-5
30-31	0.0	7.661496688701131E-5	0.0	0.0026432163576018903	7.02303863130937E-5
32-33	0.0	7.661496688701131E-5	0.0	0.0060908898675173994	7.661496688701131E-5
34-35	0.0	8.938412803484653E-5	0.0	0.010668634139016326	7.661496688701131E-5
36-37	0.0	1.0215328918268174E-4	0.0	0.017027676390638263	8.938412803484653E-5
38-39	0.0	1.0215328918268174E-4	0.0	0.026553470606923337	1.1492245033051697E-4
40-41	0.0	1.1492245033051697E-4	0.0	0.035721728311069026	1.1492245033051697E-4
42-43	0.0	1.1492245033051697E-4	0.0	0.04490275517636255	1.1492245033051697E-4
44-45	0.0	1.2130703090443458E-4	0.0	0.05437747274805628	1.1492245033051697E-4
46-47	0.0	1.2769161147835217E-4	0.0	0.06538448965749023	1.1492245033051697E-4
48-49	1.2769161147835218E-5	1.2769161147835217E-4	0.0	0.07628935327774151	1.2769161147835217E-4
50-51	1.2769161147835218E-5	1.5322993377402263E-4	0.0	0.08656852800174886	1.2769161147835217E-4
52-53	1.2769161147835218E-5	1.5322993377402263E-4	0.0	0.09641355124672982	1.404607726261874E-4
54-55	1.2769161147835218E-5	1.5322993377402263E-4	0.0	0.10598403752703231	1.5322993377402263E-4
56-57	1.2769161147835218E-5	1.5322993377402263E-4	0.0	0.11558006212963048	1.5961451434794025E-4
58-59	1.2769161147835218E-5	1.5322993377402263E-4	0.0	0.1259613901428205	1.6599909492185784E-4
60-61	1.2769161147835218E-5	1.5322993377402263E-4	0.0	0.13562126055115786	1.6599909492185784E-4
62-63	1.2769161147835218E-5	1.5322993377402263E-4	0.0	0.14446390464603376	1.6599909492185784E-4
64-65	1.2769161147835218E-5	1.6599909492185784E-4	0.0	0.15300008887336158	1.6599909492185784E-4
66-67	1.2769161147835218E-5	1.6599909492185784E-4	0.0	0.16150435019781983	1.6599909492185784E-4
68-69	1.2769161147835218E-5	1.6599909492185784E-4	0.0	0.1695808446238256	1.6599909492185784E-4
70-71	1.2769161147835218E-5	1.6599909492185784E-4	0.0	0.1765719603522654	1.7876825606969306E-4
72-73	1.2769161147835218E-5	1.6599909492185784E-4	0.0	0.18235639035223478	2.2984490066103394E-4
74-75	1.2769161147835218E-5	1.6599909492185784E-4	0.0	0.1864680602418377	2.2984490066103394E-4
76-77	1.2769161147835218E-5	1.6599909492185784E-4	0.0	0.19119903444711064	2.2984490066103394E-4
78-79	1.2769161147835218E-5	1.7876825606969306E-4	0.0	0.19395717325504305	2.2984490066103394E-4
80-81	1.2769161147835218E-5	1.7876825606969306E-4	0.0	0.19467224627932184	2.3622948123495154E-4
82-83	1.2769161147835218E-5	1.7876825606969306E-4	0.0	0.19518939730580914	2.489986423827868E-4
84-85	1.2769161147835218E-5	1.7876825606969306E-4	0.0	0.19543839594819196	2.5538322295670435E-4
86-87	1.2769161147835218E-5	1.7876825606969306E-4	0.0	0.19555331839852247	2.5538322295670435E-4
88	1.2769161147835218E-5	1.9153741721752827E-4	0.0	0.19555970297909636	2.5538322295670435E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6930	0.0	39.40884	1
GTATCAA	11875	0.0	31.395416	1
ATCAACG	12930	0.0	27.760857	3
TCAACGC	13055	0.0	27.4313	4
CAACGCA	13305	0.0	27.160385	5
AACGCAG	13745	0.0	26.320543	6
TATCAAC	14295	0.0	25.85271	2
ACGCAGA	16615	0.0	21.725065	7
GTACATG	19510	0.0	21.195362	1
CGCAGAG	17070	0.0	21.122145	8
TACATGG	19765	0.0	20.365911	2
ACATGGG	20000	0.0	19.49388	3
GCAGAGT	21075	0.0	17.146807	9
CATGGGG	15770	0.0	16.773445	4
GAGTACT	10905	0.0	16.177328	12-13
AGAGTAC	18250	0.0	15.765014	10-11
GAGTACA	14215	0.0	15.117899	1
CAGAGTA	20685	0.0	15.060087	10-11
TATACCG	550	0.0	14.798072	5
GTGGTAT	4405	0.0	14.783541	1
>>END_MODULE