FastQCFastQC Report
Fri 27 May 2016
SRR2140310_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140310_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13194855
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA118750.041.994821
GTATCAA208850.033.5155031
TCAACGC244200.027.8039534
ATCAACG244700.027.7477723
CAACGCA249950.027.1641315
AACGCAG262600.026.0589186
TATCAAC267850.025.6882082
ACGCAGA310500.021.8404487
CGCAGAG316750.021.3836468
GTACATG316400.021.2271611
TACATGG318500.020.6486912
ACATGGG323100.019.6799163
GCAGAGT388600.017.2396689
CATGGGG252400.017.2339954
AGAGTAC341700.016.6969310-11
GAGTACT221300.016.21336612-13
GTGGTAT78550.015.6363591
TGGTATC69500.015.4264622
CAGAGTA378700.015.23371310-11
CGTCGAA6753.167832E-614.70524394
Produced by FastQC (version 0.11.5)