##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140310_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13194855
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.69140865890531	31.0	31.0	34.0	30.0	34.0
2	31.804008986836156	31.0	31.0	34.0	30.0	34.0
3	31.51539277998887	31.0	31.0	34.0	30.0	34.0
4	35.679706218825444	37.0	35.0	37.0	35.0	37.0
5	35.59105477096944	37.0	35.0	37.0	33.0	37.0
6	35.642103153085046	37.0	35.0	37.0	33.0	37.0
7	35.62520596095978	37.0	35.0	37.0	33.0	37.0
8	35.617862492615494	37.0	35.0	37.0	33.0	37.0
9	37.35882031291742	39.0	37.0	39.0	34.0	39.0
10-11	37.236946370384516	39.0	37.0	39.0	33.5	39.0
12-13	37.18489324058506	39.0	37.0	39.0	33.0	39.0
14-15	38.33931801448368	40.0	38.0	41.0	33.0	41.0
16-17	38.257597487808695	40.0	38.0	41.0	33.0	41.0
18-19	38.24006690486557	40.0	38.0	41.0	33.5	41.0
20-21	38.3049660211242	40.0	38.0	41.0	34.0	41.0
22-23	38.25207686097336	40.0	38.0	41.0	33.5	41.0
24-25	38.1394099706457	40.0	37.5	41.0	33.0	41.0
26-27	37.95708409546302	40.0	37.0	41.0	33.0	41.0
28-29	37.80257827466124	40.0	37.0	41.0	33.0	41.0
30-31	37.65858575948639	40.0	37.0	41.0	32.0	41.0
32-33	37.44444271527672	39.0	36.5	41.0	32.0	41.0
34-35	37.55825857905915	39.5	37.0	41.0	32.0	41.0
36-37	37.51641629531841	40.0	36.5	41.0	32.0	41.0
38-39	37.47457008943994	39.0	36.5	41.0	32.0	41.0
40-41	37.399524449341136	39.0	36.0	41.0	31.0	41.0
42-43	37.43198077632515	39.0	36.0	41.0	31.0	41.0
44-45	37.463535465504876	40.0	36.0	41.0	32.0	41.0
46-47	37.39944450092657	39.5	36.0	41.0	31.0	41.0
48-49	37.29863198660885	39.0	36.0	41.0	31.0	41.0
50-51	37.18888746248813	39.0	36.0	41.0	31.0	41.0
52-53	36.876589137709914	39.0	35.0	40.0	30.0	41.0
54-55	36.817774942866365	39.0	35.0	40.0	30.5	41.0
56-57	36.64546417532945	38.5	35.0	40.0	30.0	41.0
58-59	36.461242106875275	38.0	35.0	40.0	30.0	41.0
60-61	36.31043578673899	38.0	35.0	40.0	30.0	41.0
62-63	36.05399068128639	38.0	34.0	40.0	29.0	41.0
64-65	35.66952492926176	37.0	34.0	40.0	29.0	41.0
66-67	35.42304717175119	37.0	34.0	39.5	28.5	41.0
68-69	35.12780718388883	36.0	34.0	39.0	28.0	41.0
70-71	34.853626019717254	36.0	33.0	39.0	28.0	40.0
72-73	34.56521104990408	35.5	33.0	38.5	28.0	40.0
74-75	34.24324804294099	35.0	33.0	38.0	28.0	40.0
76-77	33.05863383392867	34.0	31.5	36.0	26.5	39.0
78-79	33.76263041413531	35.0	32.5	37.0	28.0	39.0
80-81	33.725002189690265	35.0	33.0	36.0	28.5	39.0
82-83	33.453450574480726	35.0	33.0	36.0	28.0	37.5
84-85	33.21533200021911	35.0	32.0	35.5	28.0	37.0
86-87	33.01230229799478	35.0	32.0	35.0	27.5	37.0
88-89	32.83485854172591	34.0	32.0	35.0	27.0	36.0
90-91	32.69935135227641	34.0	32.0	35.0	27.0	36.0
92-93	32.68711268771388	34.0	32.0	35.0	27.0	36.0
94-95	32.697013808149066	34.0	32.0	35.0	28.0	35.0
96-97	32.71002873607273	34.0	32.0	35.0	29.0	35.0
98-99	32.86851586948812	34.0	32.0	35.0	29.0	35.0
100	31.57956305119856	33.0	30.0	35.0	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	4.0
19	18.0
20	111.0
21	636.0
22	3068.0
23	9970.0
24	25937.0
25	54635.0
26	97603.0
27	152394.0
28	215436.0
29	283902.0
30	356523.0
31	437658.0
32	537180.0
33	670191.0
34	872384.0
35	1172189.0
36	1729667.0
37	2606699.0
38	2731553.0
39	1212018.0
40	25077.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.194116949371555	20.823942362382912	13.795975779953626	24.185964908291908
2	15.251601130487066	21.77592790761287	40.07364279364518	22.89882816825488
3	18.273079829292897	26.15628597608251	30.769561325791823	24.801072868832765
4	12.90137072781998	18.01167352478985	38.59978332397991	30.487172423410257
5	13.045130090478448	38.02334318944771	35.98238859009818	12.94913812997566
6	30.451823835881488	38.152378332312104	18.450077700740174	12.945720131066238
7	26.763590808690207	32.44480519111426	23.23083504896416	17.56076895123137
8	24.62419632500698	36.885073765494205	21.09915569363968	17.391574215859134
9	25.523183089166196	16.391457124765676	21.222415858302345	36.862943927765784
10-11	24.348604058172675	26.666170261060085	29.094355337743387	19.890870343023852
12-13	24.88441138610466	24.15780241616903	29.237892345160294	21.71989385256602
14-15	21.9089924546262	25.738133239963503	26.538086100324065	25.81478820508623
16-17	21.74777101035114	28.877506394837337	27.3090428227403	22.065679772071224
18-19	21.78678604255794	28.293238973691103	28.720030218367725	21.199944765383236
20-21	22.44736579031596	27.473842150462364	28.600880855204586	21.47791120401709
22-23	22.23001789766981	27.411973750690688	28.41806058055943	21.939947771080075
24-25	22.060986383478937	27.491642450015437	28.48833127408397	21.959039892421657
26-27	22.08441204359694	27.64749434470037	28.338801934022772	21.929291677679913
28-29	22.049906773072905	27.798037679469427	28.32188494521779	21.830170602239875
30-31	22.22596690007295	27.62150476171934	28.30903065716168	21.843497681046024
32-33	22.053165586551877	27.704042216212304	28.26260311287579	21.980189084360028
34-35	22.15236705370276	27.76040601065015	28.27455278338375	21.81267415226334
36-37	22.176373994153934	27.71412253624898	28.173209220797357	21.936294248799726
38-39	22.075391465966575	27.784381165510318	28.251035382367867	21.889191986155232
40-41	22.288995336534338	27.668879788757522	28.123753965019755	21.918370909688385
42-43	22.17057893455858	27.767941222777587	27.973041539085692	22.088438303578144
44-45	22.231167452063445	27.839590635571977	27.846556478389772	22.08268543397481
46-47	22.225682195103172	27.832905615144902	27.678032158382376	22.26338003136955
48-49	22.228151425353964	27.882884022249165	27.68570595731023	22.20325859508664
50-51	22.2011529810333	27.97955005622249	27.583580533289933	22.235716429454282
52-53	22.31447383244513	27.925170643880847	27.50818026749135	22.252175256182674
54-55	22.314966206123113	27.82019198346803	27.584376349401314	22.280465461007548
56-57	22.314396742560398	27.92716679427748	27.541049569593813	22.217386893568307
58-59	22.316495139722996	27.99191713463996	27.57978746046934	22.111800265167698
60-61	22.407567828066256	27.92698890546396	27.52673175186024	22.138711514609547
62-63	22.370301940968538	28.014126265660384	27.44654614263929	22.169025650731797
64-65	22.296530846117857	27.9572168402332	27.511278619244727	22.23497369440421
66-67	22.330591860823485	27.94988295662426	27.4071235658711	22.312401616681154
68-69	22.25344991604427	27.9451063700613	27.505238692618217	22.296205021276215
70-71	22.25497643972515	27.774140026045025	27.52999465145203	22.440888882777795
72-73	22.346164193467978	27.77668727035631	27.51765045857928	22.359498077596427
74-75	22.18133912595839	27.957916257548547	27.504280466054276	22.356464150438786
76-77	22.315812687916385	27.85011230646769	27.42824341778416	22.405831587831766
78-79	22.234573611117973	27.812769485311755	27.435633709145396	22.517023194424876
80-81	22.23804525532983	27.88921945452255	27.494792859564043	22.377942430583573
82-83	22.276984914222044	27.873680582100768	27.589141654660708	22.26019284901648
84-85	22.3846734100685	27.857165512680183	27.488340661530163	22.269820415721156
86-87	22.222004367163994	27.87945125215566	27.53948324676448	22.359061133915866
88-89	22.28913044716503	27.866684415412635	27.495901579687615	22.34828355773472
90-91	22.27712045288811	27.963881365247612	27.49906643822872	22.25993174363556
92-93	22.29328134749571	27.882962809098654	27.577778027452055	22.245977815953584
94-95	22.104521866054093	27.976089487579298	27.655961200624425	22.263427445742185
96-97	22.107102181308523	28.12485282043037	27.723230950874562	22.044814047386545
98-99	23.215126720114228	30.08618230671582	27.933859697008085	18.76483127616187
100	29.69668283832288	41.518700394868894	28.784616766808224	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2202.0
1	1995.5
2	2774.0
3	3944.5
4	5095.0
5	8206.0
6	11097.5
7	12908.5
8	13993.5
9	13602.0
10	12299.0
11	10961.0
12	11358.0
13	14790.5
14	23221.0
15	35096.0
16	45560.0
17	52901.5
18	56837.0
19	57480.5
20	56397.0
21	55267.0
22	56096.0
23	61534.5
24	72935.0
25	90357.0
26	113922.5
27	142092.5
28	173086.5
29	208289.0
30	243841.0
31	277220.0
32	309327.5
33	339744.5
34	369315.0
35	399460.5
36	430495.5
37	459289.5
38	481327.5
39	496998.5
40	514786.0
41	534455.5
42	547772.0
43	557880.5
44	569111.5
45	577039.5
46	579901.0
47	581379.5
48	578778.0
49	568096.0
50	555839.0
51	541738.5
52	519138.5
53	491708.0
54	462015.5
55	428436.5
56	393090.0
57	356156.0
58	317184.0
59	271691.5
60	223664.5
61	181301.0
62	145265.0
63	113566.5
64	85825.0
65	65102.0
66	49400.5
67	37861.0
68	29050.5
69	21524.5
70	15953.0
71	11817.0
72	8669.5
73	6191.5
74	4373.5
75	3068.0
76	2111.5
77	1436.0
78	953.0
79	630.5
80	413.0
81	273.0
82	192.5
83	126.5
84	90.5
85	66.5
86	49.0
87	37.5
88	26.5
89	15.5
90	11.0
91	11.5
92	10.0
93	6.5
94	3.0
95	2.0
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.006790525549541847
3	0.0024630812540190855
4	3.031484620331182E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	1.1368067326241934E-4
16-17	2.387294138510806E-4
18-19	0.0017506823682412577
20-21	8.336975075627733E-5
22-23	0.0016751662403520185
24-25	2.502027210683201E-4
26-27	0.0025903925639424942
28-29	0.005541684474705246
30-31	0.0028138161720205985
32-33	0.0021169298201147465
34-35	0.00225195535215105
36-37	0.00263884667376266
38-39	0.0
40-41	6.467140615312762E-4
42-43	0.005839996673259348
44-45	0.003982607998852241
46-47	5.542578818707345E-4
48-49	0.015043123757847613
50-51	0.01058222946642066
52-53	0.014858654696149916
54-55	0.01245892724581983
56-57	0.010716024330201497
58-59	0.01680838642558169
60-61	0.008711792528027121
62-63	0.01967048380459079
64-65	0.013935863542314039
66-67	0.02907238889023693
68-69	0.019232429387757273
70-71	0.019743085380275464
72-73	0.018167291874902153
74-75	0.008941004896545857
76-77	0.01076983733347706
78-79	0.011515949859505412
80-81	0.00828867074126014
82-83	0.003498224537795196
84-85	0.002293227725685019
86-87	0.011316808131090338
88-89	9.809531594866345E-4
90-91	8.057200970280369E-5
92-93	5.754654419300563E-4
94-95	0.002126326099542021
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	384.0
20-21	1240.0
22-23	2697.0
24-25	5464.0
26-27	8496.0
28-29	18068.0
30-31	38682.0
32-33	49414.0
34-35	68086.0
36-37	99840.0
38-39	117250.0
40-41	117984.0
42-43	127584.0
44-45	144435.0
46-47	142850.0
48-49	134468.0
50-51	125311.0
52-53	121727.0
54-55	130373.0
56-57	135507.0
58-59	133293.0
60-61	131012.0
62-63	128265.0
64-65	133069.0
66-67	138398.0
68-69	140080.0
70-71	138721.0
72-73	132455.0
74-75	134566.0
76-77	134553.0
78-79	143540.0
80-81	147214.0
82-83	144259.0
84-85	146562.0
86-87	156819.0
88-89	168814.0
90-91	181872.0
92-93	201037.0
94-95	255163.0
96-97	533035.0
98-99	2706020.0
100-101	5476248.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.12168316328943
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.86738500109949	73.86806619611453
2	7.304192265828943	12.142735105679654
3	2.008671414631295	5.008924467184167
4	0.7870144232259368	2.616718541293012
5	0.4093143383297646	1.7011448372419076
6	0.22366618154912163	1.1154905686241317
7	0.12704390610697303	0.7392072317875353
8	0.07464350407645687	0.49635949568327614
9	0.04872051328978971	0.36447579623040594
>10	0.1473949930327768	1.7980883593641406
>50	0.0015678657092107824	0.08722429740451655
>100	3.699141533605428E-4	0.05323725847682445
>500	1.5678966854041048E-5	0.008327844915931937
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.515742310165591E-5	0.0	0.0	0.0
3	0.0	1.515742310165591E-5	0.0	0.0	7.578711550827955E-6
4	0.0	8.33658270591075E-5	0.0	0.0	7.578711550827955E-6
5	0.0	1.364168079149032E-4	0.0	0.0	7.578711550827955E-6
6	8.33658270591075E-5	1.364168079149032E-4	0.0	0.0	7.578711550827955E-6
7	8.33658270591075E-5	1.4399551946573115E-4	0.0	2.2736134652483866E-5	7.578711550827955E-6
8	9.094453860993546E-5	1.515742310165591E-4	0.0	3.031484620331182E-5	1.515742310165591E-5
9	9.094453860993546E-5	2.0462521187235478E-4	0.0	1.66731654118215E-4	3.031484620331182E-5
10-11	9.473389438534945E-5	2.0462521187235478E-4	0.0	1.856784329952849E-4	3.031484620331182E-5
12-13	9.852325016076342E-5	2.0462521187235478E-4	0.0	2.0841456764776877E-4	3.4104201978725795E-5
14-15	1.0231260593617739E-4	2.576761927281505E-4	0.0	2.576761927281505E-4	6.0629692406623646E-5
16-17	1.515742310165591E-4	2.6525490427897844E-4	0.0	3.1830588513477413E-4	7.578711550827955E-5
18-19	1.5915294256738707E-4	2.879910389314623E-4	0.0	3.599887986643279E-4	1.0610196171159138E-4
20-21	1.5915294256738707E-4	3.1072717358394616E-4	0.0	3.9788235641846767E-4	1.1368067326241934E-4
22-23	1.5915294256738707E-4	3.44831375562672E-4	0.0	4.357759141726074E-4	1.1747002903783331E-4
24-25	1.6294229834280105E-4	3.9788235641846767E-4	0.0	5.229310970071289E-4	1.2125938481324728E-4
26-27	1.66731654118215E-4	4.0925042374470957E-4	0.0	6.290330587187203E-4	1.2125938481324728E-4
28-29	1.66731654118215E-4	4.0925042374470957E-4	0.0	9.89021857383048E-4	1.2504874058866125E-4
30-31	1.66731654118215E-4	4.6988011615133323E-4	0.0	0.002387294138510806	1.2883809636407524E-4
32-33	1.66731654118215E-4	4.6988011615133323E-4	0.0	0.005202785479643391	1.515742310165591E-4
34-35	1.66731654118215E-4	5.418778758841988E-4	0.0	0.008654888591045524	1.5915294256738707E-4
36-37	1.66731654118215E-4	6.138756356170644E-4	0.0	0.013789465666731466	1.66731654118215E-4
38-39	1.70521009893629E-4	6.214543471678923E-4	0.0	0.02214120579574387	1.66731654118215E-4
40-41	1.7431036566904298E-4	6.82084039574516E-4	0.0	0.030602837242243282	1.66731654118215E-4
42-43	1.7431036566904298E-4	7.048201742269999E-4	0.0	0.03838996336071901	1.70521009893629E-4
44-45	1.7809972144445694E-4	8.071327801631772E-4	0.0	0.046923592566951285	1.7431036566904298E-4
46-47	1.8188907721987093E-4	8.791305398960428E-4	0.0	0.0557376341005642	1.7809972144445694E-4
48-49	1.8188907721987093E-4	8.829198956714567E-4	0.0	0.06421821232594069	1.8946778877069887E-4
50-51	2.0841456764776877E-4	9.208134534255965E-4	0.0	0.07270257990709258	2.008358560969408E-4
52-53	2.1220392342318276E-4	9.359708765272524E-4	0.0	0.08127031331530357	2.1599327919859674E-4
54-55	2.1599327919859674E-4	9.96600568933876E-4	0.0	0.09014498454132311	2.3115070230025263E-4
56-57	2.197826349740107E-4	0.001030704770912602	0.0	0.09871650730530954	2.4251876962649456E-4
58-59	2.197826349740107E-4	0.0010572302613404997	0.0	0.1071402451940548	2.500974811773225E-4
60-61	2.690442600543924E-4	0.0010648089728913278	0.0	0.11505242005311919	2.500974811773225E-4
62-63	3.3346330823643E-4	0.0010761770402175697	0.0	0.12260839546929467	2.576761927281505E-4
64-65	3.3346330823643E-4	0.0011330173768487792	0.0	0.13019847508744886	2.576761927281505E-4
66-67	3.37252664011844E-4	0.0011481747999504354	0.0	0.1376369804745865	2.576761927281505E-4
68-69	3.41042019787258E-4	0.001193647069255403	0.0	0.14496559454423713	2.6525490427897844E-4
70-71	3.713568659905698E-4	0.0012391193385603706	0.0	0.1510361424964503	2.842016831560483E-4
72-73	4.395652699480214E-4	0.0012656448289882685	0.0	0.15600777727379345	3.3346330823643E-4
74-75	4.4714398149884937E-4	0.0012808022520899246	0.0	0.15955840363535634	3.3346330823643E-4
76-77	4.5472269304967734E-4	0.0012959596751915803	0.0	0.1626315711692171	3.37252664011844E-4
78-79	4.5472269304967734E-4	0.0013186958098440641	0.0	0.16518938631762153	3.44831375562672E-4
80-81	4.5472269304967734E-4	0.0013186958098440641	0.0	0.16601546587666177	3.599887986643279E-4
82-83	4.5472269304967734E-4	0.0013186958098440641	0.0	0.16670512862778714	3.713568659905698E-4
84-85	4.5472269304967734E-4	0.0013376425887211341	0.0	0.16701585580137107	3.713568659905698E-4
86-87	4.585120488250913E-4	0.0013869042138015158	0.0	0.16718258745548928	3.8651428909222576E-4
88	4.6230140460050526E-4	0.0014172190600048277	0.0	0.16719395552281552	4.0167171219388165E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11875	0.0	41.99482	1
GTATCAA	20885	0.0	33.515503	1
TCAACGC	24420	0.0	27.803953	4
ATCAACG	24470	0.0	27.747772	3
CAACGCA	24995	0.0	27.164131	5
AACGCAG	26260	0.0	26.058918	6
TATCAAC	26785	0.0	25.688208	2
ACGCAGA	31050	0.0	21.840448	7
CGCAGAG	31675	0.0	21.383646	8
GTACATG	31640	0.0	21.227161	1
TACATGG	31850	0.0	20.648691	2
ACATGGG	32310	0.0	19.679916	3
GCAGAGT	38860	0.0	17.239668	9
CATGGGG	25240	0.0	17.233995	4
AGAGTAC	34170	0.0	16.69693	10-11
GAGTACT	22130	0.0	16.213366	12-13
GTGGTAT	7855	0.0	15.636359	1
TGGTATC	6950	0.0	15.426462	2
CAGAGTA	37870	0.0	15.233713	10-11
CGTCGAA	675	3.167832E-6	14.705243	94
>>END_MODULE