FastQCFastQC Report
Fri 27 May 2016
SRR2140309_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140309_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3390443
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38200.042.893741
GTATCAA65350.032.7346041
ATCAACG73600.028.1083343
TCAACGC74050.027.9366974
CAACGCA74200.027.8244615
AACGCAG77350.026.9587866
TATCAAC80400.026.0924022
ACGCAGA90450.022.8713387
CGCAGAG91750.022.4574158
GTACATG94150.020.2601551
TACATGG92050.019.8682962
ACATGGG91550.019.5691723
GCAGAGT108450.018.9229419
GAGTACT60750.018.45761312-13
CATGGGG66600.018.2643074
TATACCG2504.6387868E-818.2046685
AGAGTAC97000.017.08312410-11
CAGAGTA108600.016.74424610-11
ATGGGGG44400.016.4006025
ACCGAAC3808.367351E-1116.3321828
Produced by FastQC (version 0.11.5)