##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140309_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3390443
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.995174081970998	31.0	31.0	34.0	30.0	34.0
2	32.10505854249725	33.0	31.0	34.0	30.0	34.0
3	31.837087365869298	31.0	31.0	34.0	30.0	34.0
4	35.89767797305544	37.0	35.0	37.0	35.0	37.0
5	35.83600815586636	37.0	35.0	37.0	35.0	37.0
6	35.88113765664251	37.0	35.0	37.0	35.0	37.0
7	35.86818271240661	37.0	35.0	37.0	35.0	37.0
8	35.86685840168969	37.0	35.0	37.0	35.0	37.0
9	37.6404053983506	39.0	37.0	39.0	35.0	39.0
10-11	37.55131822006741	39.0	37.0	39.0	35.0	39.0
12-13	37.50524592214056	39.0	37.0	39.0	35.0	39.0
14-15	38.75653845234974	40.0	38.0	41.0	34.5	41.0
16-17	38.699893199797195	40.0	38.0	41.0	34.0	41.0
18-19	38.70496687306054	40.0	38.0	41.0	34.0	41.0
20-21	38.765788353130354	40.0	38.0	41.0	34.0	41.0
22-23	38.72529845905234	40.0	38.0	41.0	34.0	41.0
24-25	38.635780821566215	40.0	38.0	41.0	34.0	41.0
26-27	38.487586669167285	40.0	38.0	41.0	34.0	41.0
28-29	38.36138487415573	40.0	38.0	41.0	34.0	41.0
30-31	38.23790315259719	40.0	38.0	41.0	34.0	41.0
32-33	38.05155688525626	40.0	37.5	41.0	33.0	41.0
34-35	38.140943037826176	40.0	38.0	41.0	33.0	41.0
36-37	38.10082848547077	40.0	37.5	41.0	33.0	41.0
38-39	38.06636367471607	40.0	37.0	41.0	33.0	41.0
40-41	38.01175639984552	40.0	37.0	41.0	33.0	41.0
42-43	38.0392994871771	40.0	37.0	41.0	33.0	41.0
44-45	38.06980724184423	40.0	37.0	41.0	33.0	41.0
46-47	38.02149926632068	40.0	37.0	41.0	33.0	41.0
48-49	37.937217155350645	40.0	37.0	41.0	33.0	41.0
50-51	37.84853613887415	40.0	37.0	41.0	33.0	41.0
52-53	37.58930156590197	40.0	36.5	41.0	32.0	41.0
54-55	37.54234752500264	39.5	36.0	41.0	32.0	41.0
56-57	37.38220919402835	39.0	36.0	41.0	31.5	41.0
58-59	37.22275688168456	39.0	36.0	41.0	31.0	41.0
60-61	37.08514912216418	39.0	35.0	41.0	31.0	41.0
62-63	36.852330223608476	39.0	35.0	40.0	31.0	41.0
64-65	36.51026740071316	38.0	35.0	40.0	31.0	41.0
66-67	36.27880667978282	38.0	35.0	40.0	30.0	41.0
68-69	35.99945380877143	37.0	34.5	40.0	30.0	41.0
70-71	35.73193561546497	37.0	34.0	39.0	30.0	41.0
72-73	35.440881207427246	36.0	34.0	39.0	30.0	41.0
74-75	35.116050888796366	36.0	34.0	39.0	29.5	40.0
76-77	33.86537781228135	34.5	32.5	37.0	28.5	39.0
78-79	34.47982609387658	35.0	34.0	37.0	29.0	39.0
80-81	34.40181124024248	35.0	34.0	37.0	29.0	39.0
82-83	34.11779801577192	35.0	34.0	36.5	29.0	39.0
84-85	33.86353519511629	35.0	33.0	36.0	29.0	37.0
86-87	33.64630879480765	35.0	33.0	36.0	29.0	37.0
88-89	33.45991636023513	35.0	33.0	35.0	29.0	36.5
90-91	33.313954850076854	35.0	33.0	35.0	29.0	36.0
92-93	33.27128998368869	35.0	33.0	35.0	29.0	36.0
94-95	33.25795846868398	35.0	33.0	35.0	29.0	36.0
96-97	33.238763291435106	35.0	33.0	35.0	29.0	35.5
98-99	33.341978317558166	35.0	33.0	35.0	29.5	35.0
100	32.207583040025696	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	18.0
21	103.0
22	481.0
23	1636.0
24	4269.0
25	9455.0
26	17300.0
27	27727.0
28	40592.0
29	54889.0
30	69889.0
31	88327.0
32	109188.0
33	138704.0
34	183416.0
35	252331.0
36	384985.0
37	653530.0
38	859059.0
39	477024.0
40	17517.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.26286741880044	20.794834185385213	13.780647543698569	24.161650852115784
2	14.772003928983333	21.568820626572553	40.94773449275704	22.711440951687074
3	18.11241840861027	26.29137822715515	30.720570321233375	24.875633043001205
4	12.41461631279327	18.055993471036576	39.19690446435866	30.332485751811493
5	12.848999378547287	38.13256851685753	36.1261640440497	12.892268060545481
6	29.75870704801703	38.480930073149736	18.78851819658965	12.971844682243589
7	26.38761365402692	32.673281928054834	23.367418358013982	17.571686059904266
8	24.173212763051907	37.14939316189654	21.44640095704308	17.230993118008474
9	25.18490946463338	16.813053633404248	21.655252720662165	36.3467841813002
10-11	23.979668733554877	27.031880494672823	29.215651170068334	19.772799601703966
12-13	24.566627428923006	24.46805623925841	29.429900458435664	21.535415873382917
14-15	21.616938339981687	26.06062608125763	26.902523080716552	25.419912498044134
16-17	21.365630678200883	29.268469651447226	27.586162654421624	21.779737015930266
18-19	21.361449034699497	28.548310742553596	29.167958305743134	20.922281917003772
20-21	22.06955024634167	27.722718104221393	29.069113560804073	21.138618088632857
22-23	21.79617089212514	27.710809787110524	28.93418385287239	21.558835467891942
24-25	21.581540336256566	27.835104017065838	29.025183467324045	21.558172179353555
26-27	21.672006702927455	28.073083988643276	28.808168550819584	21.446740757609682
28-29	21.511085167075127	28.178802527810404	28.81419671828952	21.495915586824946
30-31	21.89529353807651	27.909924012277248	28.808993925155296	21.38578852449094
32-33	21.500170644993542	28.0925897912579	28.786214556648872	21.621025007099693
34-35	21.688171879523107	28.13106031267897	28.852059578398254	21.32870822939967
36-37	21.641843079903172	28.081490401881343	28.77160845655906	21.505058061656424
38-39	21.528399512447454	28.100530419792058	28.853194577527795	21.517875490232694
40-41	21.761101168531205	27.98749432337014	28.700140267692774	21.551264240405885
42-43	21.723939082590633	28.069161050659076	28.559222323339373	21.647677543410918
44-45	21.75933581530633	28.216607420071593	28.42069286596256	21.603363898659516
46-47	21.823636673574583	28.133069368327284	28.19285851351739	21.85043544458074
48-49	21.733044340653894	28.16843397867877	28.217846243695554	21.880675436971785
50-51	21.662444773431506	28.298091278926734	28.161228149628215	21.87823579801354
52-53	21.807630386395402	28.230421289668627	28.132248616435852	21.82969970750012
54-55	21.81428550058005	28.20073143230635	28.092925142356744	21.892057924756863
56-57	21.883574619127383	28.310384594932337	27.997078647204276	21.808962138736007
58-59	21.77382573276272	28.491729016519784	28.111916151785326	21.62252909893217
60-61	21.900947237755723	28.4770913125232	27.957942616453686	21.66401883326739
62-63	21.79135983624178	28.542416791266184	27.879554813276002	21.78666855921603
64-65	21.75885857401332	28.44748572027286	27.946841692546222	21.846814013167602
66-67	21.81592342529295	28.423372534233373	27.870021511488037	21.89068252898564
68-69	21.793366990025813	28.419345869685873	27.94391927805077	21.84336786223754
70-71	21.760296736888918	28.201951624563833	28.011067086553787	22.026684551993466
72-73	21.871253584479202	28.231227831476463	28.009161971691025	21.88835661235331
74-75	21.760968851520968	28.35257880889933	27.972777328750485	21.913675010829213
76-77	21.792383668087933	28.304653276482618	27.897920978098067	22.00504207733138
78-79	21.802376038579606	28.289538173814847	27.927075284662084	21.981010502943466
80-81	21.762033426306417	28.29391686679889	27.94685095220722	21.997198754687474
82-83	21.80758442428368	28.283763123578325	28.05716771045405	21.851484741683944
84-85	21.932251189002745	28.29111876205495	27.929862974108953	21.846767074833352
86-87	21.735501300214903	28.370418548591577	27.99987819814455	21.894201953048967
88-89	21.831569162457303	28.300011750819632	27.97734878088275	21.89107030584032
90-91	21.835988540350883	28.337446427740076	28.0185098477386	21.808055184170442
92-93	21.84879752472242	28.32708262183985	28.062506141357947	21.76161371207979
94-95	21.661444453686464	28.426254261817203	28.08788294130749	21.82441834318885
96-97	21.657853106356367	28.542685000038265	28.212496269223745	21.58696562438162
98-99	22.73009178418449	30.49788940658036	28.40960472600584	18.362414083229307
100	21.735900143613176	31.6123806145587	22.084464469205493	24.567254772622633
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	622.0
1	546.0
2	767.0
3	1136.0
4	1547.0
5	2517.5
6	3369.5
7	3900.0
8	4211.5
9	4080.0
10	3620.5
11	3214.5
12	3469.0
13	4689.5
14	7369.5
15	10903.5
16	13902.0
17	15867.5
18	16647.0
19	16494.5
20	15892.5
21	15287.5
22	15497.0
23	17273.0
24	20601.0
25	25563.0
26	32066.5
27	39745.0
28	48030.0
29	56981.0
30	65995.0
31	74723.0
32	83226.0
33	91354.0
34	99776.0
35	107660.5
36	115793.0
37	123359.5
38	128602.5
39	132595.5
40	136672.5
41	139661.5
42	141480.0
43	143817.0
44	145504.0
45	147232.5
46	148198.0
47	148056.5
48	146345.0
49	142372.5
50	137973.5
51	132537.5
52	125686.0
53	117733.0
54	109822.5
55	101143.5
56	92275.5
57	82572.0
58	72499.5
59	63151.0
60	52796.0
61	42876.5
62	34264.0
63	27006.5
64	20694.0
65	15687.5
66	11889.5
67	9091.5
68	7080.5
69	5236.0
70	3836.5
71	2835.0
72	2072.5
73	1508.5
74	1067.0
75	748.0
76	501.0
77	324.0
78	211.5
79	127.0
80	74.0
81	48.0
82	36.0
83	26.0
84	17.0
85	12.0
86	9.5
87	7.5
88	5.0
89	4.0
90	3.5
91	2.5
92	2.0
93	1.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.007462151701119883
3	0.002153110965145263
4	3.539360490649747E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	1.327260183993655E-4
16-17	2.801993721764383E-4
18-19	0.0014157441962598988
20-21	4.424370257259433E-5
22-23	0.0016224270151207248
24-25	2.3604143176241368E-4
26-27	0.002553234195037575
28-29	0.005021809422923201
30-31	0.0026784181647064396
32-33	0.002197033855846374
34-35	0.0018781492538381321
36-37	0.0025790853273886414
38-39	0.0
40-41	7.629820635124581E-4
42-43	0.006131359918683995
44-45	0.003799111692949565
46-47	6.301846472525683E-4
48-49	0.016064374794613465
50-51	0.010728789256132446
52-53	0.01515001854290669
54-55	0.012853565508393856
56-57	0.01141490487496193
58-59	0.01715509974405129
60-61	0.008666739399303194
62-63	0.01972353039511145
64-65	0.014193874778405297
66-67	0.030555544811693106
68-69	0.02083973637644501
70-71	0.020466388640866046
72-73	0.018995703361492065
74-75	0.008924427615055509
76-77	0.011847160804591996
78-79	0.013162596018966225
80-81	0.010023940766927118
82-83	0.00436076851083244
84-85	0.002866403978411659
86-87	0.013651089301207505
88-89	0.0013931190016384291
90-91	2.8712618478005623E-4
92-93	0.0011682786478092585
94-95	0.003233419947861103
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	86.0
20-21	275.0
22-23	554.0
24-25	1265.0
26-27	2049.0
28-29	4960.0
30-31	10159.0
32-33	12838.0
34-35	17638.0
36-37	26061.0
38-39	30566.0
40-41	30570.0
42-43	32942.0
44-45	37414.0
46-47	36863.0
48-49	34421.0
50-51	31720.0
52-53	30153.0
54-55	32343.0
56-57	33352.0
58-59	33552.0
60-61	32564.0
62-63	31989.0
64-65	33253.0
66-67	34784.0
68-69	34260.0
70-71	33223.0
72-73	31149.0
74-75	31753.0
76-77	31740.0
78-79	33580.0
80-81	34082.0
82-83	33239.0
84-85	33780.0
86-87	35441.0
88-89	37932.0
90-91	40491.0
92-93	44282.0
94-95	54941.0
96-97	123768.0
98-99	216625.0
100-101	1967786.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.01490790034676
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.8445341618774	81.75532749569768
2	6.0971975821931785	10.854829624582853
3	1.2672930448380995	3.384239210070403
4	0.42070203521527155	1.4979501167270333
5	0.15899716744256265	0.7076559108157863
6	0.07792730793255835	0.41620152831231855
7	0.041229646081773534	0.25690372041130743
8	0.027189450393696395	0.1936213138124745
9	0.0162337169013239	0.13005385333664843
>10	0.04697345729502747	0.618153865779671
>50	9.125621855104811E-4	0.055391910456881396
>100	8.098676435550849E-4	0.12967144999694408
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	2.949467075541456E-5	0.0	0.0	2.949467075541456E-5
5	0.0	2.949467075541456E-5	0.0	0.0	2.949467075541456E-5
6	0.0	2.949467075541456E-5	0.0	0.0	2.949467075541456E-5
7	0.0	2.949467075541456E-5	0.0	5.898934151082912E-5	2.949467075541456E-5
8	0.0	2.949467075541456E-5	0.0	5.898934151082912E-5	2.949467075541456E-5
9	0.0	1.4747335377707278E-4	0.0	1.1797868302165824E-4	5.898934151082912E-5
10-11	7.373667688853639E-5	1.4747335377707278E-4	0.0	1.1797868302165824E-4	5.898934151082912E-5
12-13	8.848401226624367E-5	1.4747335377707278E-4	0.0	1.1797868302165824E-4	8.848401226624367E-5
14-15	8.848401226624367E-5	3.0969404293185285E-4	0.0	1.327260183993655E-4	1.4747335377707278E-4
16-17	8.848401226624367E-5	3.2444137830956013E-4	0.0	1.6222068915478007E-4	1.7696802453248735E-4
18-19	1.0323134764395095E-4	3.8343071982038925E-4	0.0	2.064626952879019E-4	1.7696802453248735E-4
20-21	1.1797868302165824E-4	4.424200613312184E-4	0.0	2.507047014210237E-4	1.7696802453248735E-4
22-23	1.1797868302165824E-4	4.7191473208663294E-4	0.0	2.9494670755414557E-4	1.9171535991019463E-4
24-25	1.1797868302165824E-4	5.30904073597462E-4	0.0	3.8343071982038925E-4	2.064626952879019E-4
26-27	1.1797868302165824E-4	5.603987443528766E-4	0.0	4.424200613312184E-4	2.064626952879019E-4
28-29	1.4747335377707278E-4	5.603987443528766E-4	0.0	9.143347934178513E-4	2.064626952879019E-4
30-31	1.4747335377707278E-4	5.603987443528766E-4	0.0	0.0023743209958108718	2.064626952879019E-4
32-33	1.7696802453248735E-4	5.603987443528766E-4	0.0	0.005279546065219206	2.064626952879019E-4
34-35	1.7696802453248735E-4	6.046407504859985E-4	0.0	0.009423547306354951	2.65452036798731E-4
36-37	1.7696802453248735E-4	6.488827566191203E-4	0.0	0.015897627537168446	2.65452036798731E-4
38-39	1.7696802453248735E-4	6.488827566191203E-4	0.0	0.02548339553267818	2.65452036798731E-4
40-41	1.7696802453248735E-4	7.37366768885364E-4	0.0	0.03517239487583186	3.68683384442682E-4
42-43	1.7696802453248735E-4	7.37366768885364E-4	0.0	0.04378483873641291	3.8343071982038925E-4
44-45	2.064626952879019E-4	8.111034457739003E-4	0.0	0.053459090744188885	3.8343071982038925E-4
46-47	2.064626952879019E-4	8.700927872847294E-4	0.0	0.06391495152698334	3.8343071982038925E-4
48-49	2.064626952879019E-4	8.848401226624368E-4	0.0	0.07366294021164785	4.424200613312184E-4
50-51	2.064626952879019E-4	9.733241349286804E-4	0.0	0.0836468862623557	4.7191473208663294E-4
52-53	2.064626952879019E-4	0.0010175661410618021	0.0	0.09311467557484376	4.7191473208663294E-4
54-55	2.064626952879019E-4	0.0010323134764395096	0.0	0.10236125485666622	5.161567382197547E-4
56-57	2.064626952879019E-4	0.0010323134764395096	0.0	0.11081147802809249	5.30904073597462E-4
58-59	2.064626952879019E-4	0.0010323134764395096	0.0	0.11802292502779135	5.30904073597462E-4
60-61	2.3595736604331647E-4	0.0010323134764395096	0.0	0.12527861403362334	5.30904073597462E-4
62-63	2.3595736604331647E-4	0.0010323134764395096	0.0	0.13299147043616424	5.30904073597462E-4
64-65	2.3595736604331647E-4	0.0011502921594611678	0.0	0.1405863481556835	5.30904073597462E-4
66-67	2.3595736604331647E-4	0.0011502921594611678	0.0	0.1469277023680976	5.456514089751693E-4
68-69	2.3595736604331647E-4	0.0011502921594611678	0.0	0.15357875062344362	5.603987443528766E-4
70-71	2.3595736604331647E-4	0.001165039494838875	0.0	0.1598611154943469	5.603987443528766E-4
72-73	2.3595736604331647E-4	0.0011797868302165823	0.0	0.16458026281521324	6.193880858637057E-4
74-75	2.3595736604331647E-4	0.0011945341655942897	0.0	0.16826709665964004	6.193880858637057E-4
76-77	2.507047014210237E-4	0.001209281500971997	0.0	0.17186544649180063	6.193880858637057E-4
78-79	2.65452036798731E-4	0.001209281500971997	0.0	0.17432825149987774	6.193880858637057E-4
80-81	2.65452036798731E-4	0.001209281500971997	0.0	0.1748739029088529	6.193880858637057E-4
82-83	2.65452036798731E-4	0.001268270842482826	0.0	0.17534581764093954	6.193880858637057E-4
84-85	2.65452036798731E-4	0.0013567548547490696	0.0	0.1754490489885835	6.488827566191203E-4
86-87	2.65452036798731E-4	0.0013862495255044843	0.0	0.1754932909947166	6.488827566191203E-4
88	2.65452036798731E-4	0.001474733537770728	0.0	0.1754932909947166	6.488827566191203E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3820	0.0	42.89374	1
GTATCAA	6535	0.0	32.734604	1
ATCAACG	7360	0.0	28.108334	3
TCAACGC	7405	0.0	27.936697	4
CAACGCA	7420	0.0	27.824461	5
AACGCAG	7735	0.0	26.958786	6
TATCAAC	8040	0.0	26.092402	2
ACGCAGA	9045	0.0	22.871338	7
CGCAGAG	9175	0.0	22.457415	8
GTACATG	9415	0.0	20.260155	1
TACATGG	9205	0.0	19.868296	2
ACATGGG	9155	0.0	19.569172	3
GCAGAGT	10845	0.0	18.922941	9
GAGTACT	6075	0.0	18.457613	12-13
CATGGGG	6660	0.0	18.264307	4
TATACCG	250	4.6387868E-8	18.204668	5
AGAGTAC	9700	0.0	17.083124	10-11
CAGAGTA	10860	0.0	16.744246	10-11
ATGGGGG	4440	0.0	16.400602	5
ACCGAAC	380	8.367351E-11	16.332182	8
>>END_MODULE