FastQCFastQC Report
Fri 27 May 2016
SRR2140308_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140308_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8215405
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA100650.036.3822171
GTATCAA163750.030.6079831
ATCAACG182750.026.6178283
CAACGCA182700.026.4894685
TCAACGC184050.026.384724
AACGCAG190150.025.6237376
TATCAAC200100.024.700162
ACGCAGA219600.022.1687727
GTACATG223850.021.805361
CGCAGAG223850.021.7113298
TACATGG222000.021.3973082
ACATGGG223450.020.8357943
CATGGGG167650.019.6684044
CTCGATA9600.018.09400694
GCAGAGT266650.018.0576789
GAGTACT150150.016.90646212-13
AGAGTAC235800.016.70742410-11
ATGGGGG117200.016.6850435
CAGAGTA256000.016.17214410-11
GTACTTT170400.014.97040514-15
Produced by FastQC (version 0.11.5)