##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140308_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8215405
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.02505439476204	33.0	31.0	34.0	30.0	34.0
2	32.13479189400888	33.0	31.0	34.0	30.0	34.0
3	31.871048597117245	31.0	31.0	34.0	30.0	34.0
4	35.924426854184304	37.0	35.0	37.0	35.0	37.0
5	35.866990489208995	37.0	35.0	37.0	35.0	37.0
6	35.908465498657705	37.0	35.0	37.0	35.0	37.0
7	35.89385270720068	37.0	35.0	37.0	35.0	37.0
8	35.89071056143915	37.0	35.0	37.0	35.0	37.0
9	37.669881886529026	39.0	37.0	39.0	35.0	39.0
10-11	37.58395550797557	39.0	37.0	39.0	35.0	39.0
12-13	37.53903331606902	39.0	37.0	39.0	35.0	39.0
14-15	38.80462837802884	40.0	38.0	41.0	35.0	41.0
16-17	38.74606181922863	40.0	38.0	41.0	34.0	41.0
18-19	38.74726942859178	40.0	38.0	41.0	34.0	41.0
20-21	38.8088671309594	40.0	38.0	41.0	34.5	41.0
22-23	38.76945482525361	40.0	38.0	41.0	34.0	41.0
24-25	38.68299930521858	40.0	38.0	41.0	34.0	41.0
26-27	38.541871332143074	40.0	38.0	41.0	34.0	41.0
28-29	38.42031503796234	40.0	38.0	41.0	34.0	41.0
30-31	38.30004608915195	40.0	38.0	41.0	34.0	41.0
32-33	38.117044449127874	40.0	37.5	41.0	33.5	41.0
34-35	38.201257035892155	40.0	38.0	41.0	34.0	41.0
36-37	38.16264816654625	40.0	38.0	41.0	33.0	41.0
38-39	38.12993865158588	40.0	38.0	41.0	33.0	41.0
40-41	38.07205208064347	40.0	37.5	41.0	33.0	41.0
42-43	38.09849343410738	40.0	37.5	41.0	33.0	41.0
44-45	38.12680492212793	40.0	37.5	41.0	33.0	41.0
46-47	38.07361547787808	40.0	37.0	41.0	33.0	41.0
48-49	37.984208484804775	40.0	37.0	41.0	33.0	41.0
50-51	37.8895151037366	40.0	37.0	41.0	33.0	41.0
52-53	37.63097024637145	40.0	36.5	41.0	32.0	41.0
54-55	37.5760877600067	39.5	36.0	41.0	32.0	41.0
56-57	37.411479615636125	39.0	36.0	41.0	32.0	41.0
58-59	37.243283012034894	39.0	35.5	41.0	32.0	41.0
60-61	37.09565327169236	39.0	35.0	41.0	31.0	41.0
62-63	36.85033667462436	38.5	35.0	40.0	31.0	41.0
64-65	36.498946491468885	38.0	35.0	40.0	31.0	41.0
66-67	36.25710729537714	37.5	35.0	40.0	31.0	41.0
68-69	35.97072458615614	37.0	34.5	40.0	30.0	41.0
70-71	35.69783837905164	36.5	34.0	39.0	30.0	41.0
72-73	35.40507554574288	36.0	34.0	39.0	30.0	40.5
74-75	35.0779032059657	35.5	34.0	38.5	30.0	40.0
76-77	33.836481517107266	34.5	32.5	37.0	28.5	39.0
78-79	34.44470919764022	35.0	34.0	37.0	29.0	39.0
80-81	34.368128680640595	35.0	34.0	37.0	30.0	39.0
82-83	34.09110516704114	35.0	34.0	36.0	29.0	38.5
84-85	33.8499564017349	35.0	33.5	36.0	29.0	37.0
86-87	33.64169615480567	35.0	33.0	35.5	29.0	37.0
88-89	33.46913017188864	35.0	33.0	35.0	29.0	36.5
90-91	33.3310779435632	35.0	33.0	35.0	29.0	36.0
92-93	33.2972497482685	35.0	33.0	35.0	29.0	36.0
94-95	33.28430518855603	35.0	33.0	35.0	29.0	36.0
96-97	33.26683603525752	35.0	33.0	35.0	29.0	35.0
98-99	33.367527825981256	35.0	33.0	35.0	29.5	35.0
100	32.290699099874	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	5.0
20	39.0
21	276.0
22	1077.0
23	3708.0
24	9711.0
25	21463.0
26	39292.0
27	63853.0
28	92329.0
29	126017.0
30	162961.0
31	205041.0
32	257610.0
33	329477.0
34	440192.0
35	607921.0
36	939023.0
37	1596176.0
38	2074358.0
39	1197986.0
40	46888.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.62674877258029	20.481278768362607	13.706494080328358	24.185478378728742
2	14.977596893572247	21.37373338483445	40.63573037097236	23.012939350620943
3	18.247751364421383	26.027643379275787	30.623828696158977	25.10077656014385
4	12.652030602642439	17.825310098669945	38.812632761839424	30.710026536848194
5	13.067243793823922	37.97174941466672	35.90268526019107	13.058321531318299
6	30.26343801675024	37.937009800490664	18.63819251759347	13.161359665165625
7	26.653415138998014	32.58321409595754	23.198272026759483	17.565098738284966
8	24.59253073950706	36.68760091559698	21.260802115050932	17.45906622984503
9	25.475530421202606	16.55142990515988	21.433088204415974	36.53995146922154
10-11	24.317723837108456	26.724701947134683	28.960933757982716	19.996640457774145
12-13	24.859492623918115	24.18675037931788	29.187197709668606	21.7665592870954
14-15	21.955332402722888	25.753049518245984	26.620378372509375	25.671239706521753
16-17	21.69650095309042	28.853181471824797	27.422225143572177	22.028092431512604
18-19	21.669130067888844	28.259950147697566	28.887872488631682	21.18304729578191
20-21	22.400736164341055	27.41409371999206	28.768382425664914	21.41678769000197
22-23	22.19896994275451	27.340825451930634	28.562713495765983	21.897491109548874
24-25	21.9692199935791	27.459094171126015	28.682133445458557	21.889552389836325
26-27	21.95994374264881	27.667500537539112	28.501840424281294	21.870715295530783
28-29	21.890838163845665	27.791000950922335	28.48122742260542	21.836933462626583
30-31	22.141945701036818	27.605080322948517	28.441847446142464	21.811126529872197
32-33	21.88884526844311	27.745685008832528	28.424811211473553	21.940658511250806
34-35	22.042209088185018	27.78057008465144	28.471299635409974	21.705921191753564
36-37	22.01014398664328	27.753772910220114	28.374718331064525	21.86136477207208
38-39	21.897463739761662	27.841085705574315	28.41470113488852	21.846749419775506
40-41	22.13407984603997	27.690234297938392	28.291817600129814	21.883868255891823
42-43	22.02006745511155	27.780824905701145	28.146258905187015	22.052848734000293
44-45	22.11281669708819	27.913380690148728	27.976064527213374	21.997738085549713
46-47	22.1134366858802	27.839083350167414	27.797680664599127	22.249799299353256
48-49	22.129672798604215	27.929476425036558	27.799373305728153	22.141477470631077
50-51	22.10512574312258	27.96982985314198	27.72819606373551	22.196848339999924
52-53	22.18957763053198	27.957099464759526	27.63834667228521	22.21497623242329
54-55	22.143928195129433	27.899919153775272	27.69605380708331	22.260098844011985
56-57	22.2137027297798	27.958803134338666	27.594723017450555	22.23277111843098
58-59	22.14749153467426	28.05907707137216	27.688097290177012	22.10533410377657
60-61	22.287371318764958	27.998038926792184	27.61055805704997	22.10403169739289
62-63	22.248342779196303	28.080106988167337	27.525533845764755	22.146016386871604
64-65	22.126904603869782	28.047466076953526	27.552027711597205	22.273601607579486
66-67	22.21772136628725	27.99247808652407	27.48955586065262	22.30024468653606
68-69	22.16156282125715	27.969195520505213	27.594476375476955	22.27476528276068
70-71	22.14327342073005	27.78815339804037	27.60275071588258	22.465822465346996
72-73	22.22742945850755	27.88868630599779	27.59409786771317	22.289786367781495
74-75	22.18775260879686	27.988426131685472	27.533487576279203	22.29033368323846
76-77	22.194705088976487	27.92243511221023	27.485064164061306	22.397795634751972
78-79	22.170138797933404	27.884688505190375	27.519091125724316	22.4260815711519
80-81	22.11862837555258	27.978450283872697	27.56406237916469	22.338858961410036
82-83	22.18166747572162	27.935594493008143	27.668120844005472	22.214617187264764
84-85	22.342325081494234	27.894356472698647	27.526801021709847	22.236517424097276
86-87	22.113851159546964	27.962237881373607	27.59912703005167	22.32478392902776
88-89	22.21103072470802	27.889386006858157	27.59093060961008	22.30865265882374
90-91	22.200690150591857	27.970573996958155	27.58034574756553	22.248390104884454
92-93	22.221300273933274	27.936952492778676	27.627836262219024	22.21391097106903
94-95	22.08538802031655	28.005378333774424	27.660430205531366	22.24880344037766
96-97	22.07929805037424	28.134636536882766	27.777311205732747	22.00875420701025
98-99	23.146639127356206	30.11203516774269	27.980238004815867	18.761087700085234
100	29.610134879660215	41.554109048085095	28.835756072254686	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1485.0
1	1358.5
2	1860.0
3	2634.5
4	3561.0
5	5931.0
6	8022.0
7	9236.5
8	9767.0
9	9264.5
10	8300.5
11	7488.5
12	8028.0
13	10608.0
14	16815.5
15	25481.0
16	32746.0
17	37234.0
18	39152.0
19	39050.0
20	37744.0
21	36325.0
22	36349.5
23	39454.5
24	46210.5
25	57117.5
26	71899.5
27	89118.5
28	107570.5
29	128524.5
30	149909.0
31	169637.0
32	189356.5
33	209937.5
34	229542.5
35	248027.0
36	267391.5
37	285372.5
38	300063.0
39	311504.0
40	322389.5
41	333638.0
42	342496.0
43	349057.0
44	357234.0
45	366019.0
46	371241.0
47	374248.0
48	372164.5
49	364175.5
50	355677.5
51	343915.5
52	327420.0
53	308275.5
54	286378.5
55	263472.0
56	241203.0
57	217784.0
58	193126.5
59	165227.5
60	136675.0
61	111473.0
62	89357.5
63	70323.5
64	53792.0
65	41070.0
66	31426.5
67	24423.0
68	18598.0
69	13605.5
70	9985.0
71	7255.0
72	5210.5
73	3689.5
74	2608.5
75	1811.0
76	1265.5
77	858.5
78	565.5
79	374.0
80	245.5
81	160.0
82	117.5
83	83.5
84	61.5
85	46.5
86	35.0
87	26.5
88	16.5
89	10.0
90	6.5
91	3.0
92	1.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.008471889091286431
3	0.002446623142742202
4	4.3820115989412576E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	1.0955028997353144E-4
16-17	3.043063610375873E-4
18-19	0.0016736849857067302
20-21	6.695054509307891E-5
22-23	0.0017774194803105443
24-25	3.044224731245186E-4
26-27	0.0025764666993506394
28-29	0.006029013269986225
30-31	0.002926058025013459
32-33	0.002121036649919468
34-35	0.0022784048112027564
36-37	0.003099083526623173
38-39	0.0
40-41	6.571668148451456E-4
42-43	0.006199580918545488
44-45	0.0038881198047259645
46-47	5.952949846920875E-4
48-49	0.01654500107827309
50-51	0.011397848640992494
52-53	0.015369583965886432
54-55	0.012811851489893018
56-57	0.010918786055463137
58-59	0.01713776469835045
60-61	0.009012516327202316
62-63	0.019772011619108062
64-65	0.014439513365156916
66-67	0.03126655198832696
68-69	0.021518363752260395
70-71	0.02109731066635056
72-73	0.01942400712529432
74-75	0.009491329705123648
76-77	0.01238509320468086
78-79	0.013103810033962757
80-81	0.009929387890250493
82-83	0.004920182883932152
84-85	0.0031931549346900547
86-87	0.013962883734910288
88-89	0.0012099281592374523
90-91	2.5989736826192246E-4
92-93	0.0010581228653473403
94-95	0.002788581281387281
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	231.0
20-21	742.0
22-23	1487.0
24-25	3033.0
26-27	5378.0
28-29	12968.0
30-31	27405.0
32-33	34069.0
34-35	46928.0
36-37	69877.0
38-39	81006.0
40-41	80528.0
42-43	86281.0
44-45	97624.0
46-47	95964.0
48-49	89168.0
50-51	82015.0
52-53	78386.0
54-55	83724.0
56-57	86799.0
58-59	85027.0
60-61	82460.0
62-63	80040.0
64-65	82930.0
66-67	86862.0
68-69	86534.0
70-71	84584.0
72-73	79645.0
74-75	81069.0
76-77	81626.0
78-79	85313.0
80-81	86728.0
82-83	84111.0
84-85	84321.0
86-87	88998.0
88-89	94888.0
90-91	100039.0
92-93	107630.0
94-95	132726.0
96-97	294727.0
98-99	1678355.0
100-101	3483179.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.20488739770533
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.47027390525393	75.33834339633762
2	6.997365768204809	11.784247931844877
3	1.9212680001337124	4.853404668362214
4	0.7570106790041032	2.549759959376039
5	0.3380825765835202	1.4234102646170703
6	0.17291931143419828	0.8736390688923243
7	0.10074862307527409	0.5938468523069117
8	0.06598372130297037	0.4444921457918552
9	0.0427949508142846	0.3243189613056456
>10	0.13119011106193268	1.600515814567665
>50	0.0017397119252640595	0.09786595205310404
>100	5.753553191563149E-4	0.08967673670326055
>500	4.728588688340578E-5	0.026478247841429978
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.4344508883006984E-5
2	0.0	0.0	0.0	0.0	2.4344508883006984E-5
3	0.0	0.0	0.0	1.2172254441503492E-5	2.4344508883006984E-5
4	0.0	2.4344508883006984E-5	0.0	2.4344508883006984E-5	2.4344508883006984E-5
5	0.0	2.4344508883006984E-5	0.0	2.4344508883006984E-5	2.4344508883006984E-5
6	0.0	2.4344508883006984E-5	0.0	2.4344508883006984E-5	6.0861272207517464E-5
7	0.0	2.4344508883006984E-5	0.0	2.4344508883006984E-5	7.303352664902096E-5
8	0.0	2.4344508883006984E-5	0.0	3.651676332451048E-5	7.303352664902096E-5
9	0.0	8.520578109052445E-5	0.0	8.520578109052445E-5	1.2172254441503493E-4
10-11	0.0	8.520578109052445E-5	0.0	9.12919083112762E-5	1.2172254441503493E-4
12-13	0.0	9.737803553202794E-5	0.0	1.0346416275277968E-4	1.2172254441503493E-4
14-15	0.0	1.582393077395454E-4	0.0	1.2172254441503493E-4	1.6432543496029716E-4
16-17	0.0	1.704115621810489E-4	0.0	1.3389479885653842E-4	1.825838166225524E-4
18-19	0.0	2.0084219828480764E-4	0.0	1.582393077395454E-4	2.1301445272631111E-4
20-21	0.0	2.3127283438856635E-4	0.0	1.825838166225524E-4	2.4344508883006986E-4
22-23	0.0	2.6170347049232507E-4	0.0	2.6778959771307683E-4	2.6778959771307683E-4
24-25	0.0	2.6778959771307683E-4	0.0	3.4690925158284956E-4	2.9213410659608384E-4
26-27	0.0	2.6778959771307683E-4	0.0	4.4428728711487747E-4	3.164786154790908E-4
28-29	0.0	2.6778959771307683E-4	0.0	8.520578109052445E-4	3.2256474269984255E-4
30-31	0.0	2.9213410659608384E-4	0.0	0.002714412740455279	3.286508699205943E-4
32-33	1.2172254441503492E-5	2.9213410659608384E-4	0.0	0.006000921439661222	3.286508699205943E-4
34-35	1.2172254441503492E-5	3.3473699714134603E-4	0.0	0.009950818005929105	3.286508699205943E-4
36-37	1.2172254441503492E-5	3.773398876866083E-4	0.0	0.016353423842159945	3.286508699205943E-4
38-39	1.2172254441503492E-5	3.773398876866083E-4	0.0	0.026158174794791003	3.4690925158284956E-4
40-41	1.2172254441503492E-5	4.5037341433562923E-4	0.0	0.03632200725344642	3.5299537880360127E-4
42-43	1.2172254441503492E-5	4.56459541556381E-4	0.0	0.04525644201350998	3.5908150602435303E-4
44-45	1.2172254441503492E-5	4.929763048808914E-4	0.0	0.05543853285382766	3.773398876866083E-4
46-47	1.2172254441503492E-5	5.234069409846501E-4	0.0	0.06599796358183194	3.773398876866083E-4
48-49	1.2172254441503492E-5	5.294930682054019E-4	0.0	0.07683127003477004	3.773398876866083E-4
50-51	1.2172254441503492E-5	5.903543404129194E-4	0.0	0.08720811694615177	3.955982693488635E-4
52-53	1.2172254441503492E-5	5.964404676336711E-4	0.0	0.09710415980709411	4.199427782318705E-4
54-55	1.2172254441503492E-5	6.025265948544228E-4	0.0	0.1070062887952572	4.5645954155638095E-4
56-57	1.2172254441503492E-5	6.086127220751746E-4	0.0	0.11510692412607777	4.8689017766013966E-4
58-59	1.2172254441503492E-5	6.086127220751746E-4	0.0	0.12297020049528903	4.990624321016432E-4
60-61	1.2172254441503492E-5	6.268711037374299E-4	0.0	0.1303526728140609	5.173208137638984E-4
62-63	1.2172254441503492E-5	6.329572309581816E-4	0.0	0.13734563299070468	5.416653226469055E-4
64-65	1.2172254441503492E-5	7.059907576072025E-4	0.0	0.14495937814386511	5.477514498676572E-4
66-67	1.2172254441503492E-5	7.120768848279543E-4	0.0	0.15210449150102764	5.477514498676572E-4
68-69	1.2172254441503492E-5	7.303352664902096E-4	0.0	0.15922526034930717	5.477514498676572E-4
70-71	1.2172254441503492E-5	7.42507520931713E-4	1.2172254441503492E-5	0.16530530144283817	5.477514498676572E-4
72-73	1.2172254441503492E-5	7.42507520931713E-4	1.2172254441503492E-5	0.17065500726987898	6.086127220751746E-4
74-75	1.2172254441503492E-5	7.668520298147201E-4	1.2172254441503492E-5	0.17473879863500338	6.086127220751746E-4
76-77	1.2172254441503492E-5	7.790242842562235E-4	1.2172254441503492E-5	0.17847568074854497	6.086127220751746E-4
78-79	1.2172254441503492E-5	7.790242842562235E-4	1.2172254441503492E-5	0.18094664840017016	6.086127220751746E-4
80-81	1.2172254441503492E-5	7.790242842562235E-4	1.2172254441503492E-5	0.18171958655720563	6.451294853996851E-4
82-83	1.2172254441503492E-5	7.790242842562235E-4	1.2172254441503492E-5	0.18211518482655448	6.938185031656991E-4
84-85	1.2172254441503492E-5	7.790242842562235E-4	1.2172254441503492E-5	0.1822916825159563	7.059907576072025E-4
86-87	1.2172254441503492E-5	7.972826659184788E-4	1.2172254441503492E-5	0.18237688829704682	7.181630120487061E-4
88	1.2172254441503492E-5	8.520578109052445E-4	1.2172254441503492E-5	0.18237688829704682	7.181630120487061E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10065	0.0	36.382217	1
GTATCAA	16375	0.0	30.607983	1
ATCAACG	18275	0.0	26.617828	3
CAACGCA	18270	0.0	26.489468	5
TCAACGC	18405	0.0	26.38472	4
AACGCAG	19015	0.0	25.623737	6
TATCAAC	20010	0.0	24.70016	2
ACGCAGA	21960	0.0	22.168772	7
GTACATG	22385	0.0	21.80536	1
CGCAGAG	22385	0.0	21.711329	8
TACATGG	22200	0.0	21.397308	2
ACATGGG	22345	0.0	20.835794	3
CATGGGG	16765	0.0	19.668404	4
CTCGATA	960	0.0	18.094006	94
GCAGAGT	26665	0.0	18.057678	9
GAGTACT	15015	0.0	16.906462	12-13
AGAGTAC	23580	0.0	16.707424	10-11
ATGGGGG	11720	0.0	16.685043	5
CAGAGTA	25600	0.0	16.172144	10-11
GTACTTT	17040	0.0	14.970405	14-15
>>END_MODULE