FastQCFastQC Report
Fri 27 May 2016
SRR2140307_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140307_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3577886
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38050.040.8146671
GTATCAA66400.030.3115271
TCAACGC71800.026.9917284
ATCAACG72050.026.8405973
CAACGCA73100.026.5117115
AACGCAG75350.025.829976
TATCAAC78350.025.4241072
GTACATG96900.023.3350351
TACATGG97150.022.6781942
ACGCAGA90400.021.3923117
ACATGGG98750.021.3446963
CGCAGAG92500.020.8618818
TGCGCGA2400.00506083119.58608894
TATACCG2202.4003748E-718.8229035
CATGGGG74150.018.373594
GCAGAGT110900.017.2138759
CTAGTAC10500.016.5641543
ACGGGCG3303.7216523E-916.3131835
AGAGTAC98850.016.0868110-11
GAGTACT61300.016.04477712-13
Produced by FastQC (version 0.11.5)