##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2140307_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3577886 Sequences flagged as poor quality 0 Sequence length 19-100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.31739720046978 33.0 31.0 34.0 31.0 34.0 2 32.417563332090516 34.0 31.0 34.0 30.0 34.0 3 32.21272896900572 33.0 31.0 34.0 30.0 34.0 4 35.45308905873468 37.0 34.0 37.0 34.0 37.0 5 35.40630416955711 37.0 34.0 37.0 34.0 37.0 6 36.18634439442732 37.0 36.0 37.0 35.0 38.0 7 36.16836310603524 37.0 35.0 37.0 35.0 38.0 8 36.141373705031405 37.0 35.0 37.0 35.0 38.0 9 37.55024643043406 39.0 37.0 39.0 35.0 39.0 10-11 37.485792867631886 39.0 37.0 39.0 35.0 39.0 12-13 37.42824030726524 39.0 37.0 39.0 35.0 39.0 14-15 38.44304919161762 40.0 38.0 41.0 35.0 41.0 16-17 38.37440754121288 40.0 38.0 41.0 35.0 41.0 18-19 38.39134575556628 39.5 38.0 41.0 34.5 41.0 20-21 38.436581676788485 40.0 38.0 41.0 35.0 41.0 22-23 38.40146827304697 39.5 38.0 41.0 35.0 41.0 24-25 38.32385044354076 39.0 38.0 41.0 34.0 41.0 26-27 38.18791119217127 39.0 38.0 41.0 34.0 41.0 28-29 38.06848086466468 39.0 38.0 41.0 34.0 41.0 30-31 37.9887603565296 39.0 38.0 41.0 34.0 41.0 32-33 37.82409852060456 39.0 37.5 41.0 34.0 41.0 34-35 37.895101746108196 39.0 38.0 41.0 34.0 41.0 36-37 37.85161647169082 39.0 38.0 41.0 34.0 41.0 38-39 37.83132751674805 39.0 37.5 41.0 34.0 41.0 40-41 37.77748947946901 39.0 37.0 41.0 33.0 41.0 42-43 37.80440857232708 39.0 37.5 41.0 33.0 41.0 44-45 37.79686199625095 39.0 37.0 41.0 33.0 41.0 46-47 37.74382902256416 39.0 37.0 41.0 33.0 41.0 48-49 37.66508554739212 39.0 37.0 41.0 33.0 41.0 50-51 37.59751334876931 38.5 37.0 41.0 33.0 41.0 52-53 37.38631096499576 38.0 37.0 40.5 33.0 41.0 54-55 37.33351481906159 38.0 37.0 40.0 33.0 41.0 56-57 37.2007066897283 38.0 36.5 40.0 32.0 41.0 58-59 37.06952762591426 38.0 36.0 40.0 32.0 41.0 60-61 36.94203899612134 38.0 36.0 40.0 32.0 41.0 62-63 36.76147994175384 38.0 35.5 40.0 31.5 41.0 64-65 36.46743224429671 38.0 35.0 40.0 31.0 41.0 66-67 36.26460855090039 38.0 35.0 39.0 31.0 41.0 68-69 36.01758293093104 37.5 35.0 39.0 31.0 41.0 70-71 35.81015643462814 37.0 35.0 39.0 31.0 40.5 72-73 35.56282527517634 37.0 34.0 38.0 30.0 40.0 74-75 35.31082031215696 36.5 34.0 38.0 30.0 39.5 76-77 34.33605578833139 35.0 33.0 38.0 28.5 39.0 78-79 34.80918036061628 36.0 34.0 38.0 30.0 39.0 80-81 34.74002278297888 35.0 34.0 38.0 30.0 38.5 82-83 34.526220005005634 35.0 34.0 37.0 30.0 38.0 84-85 34.33419289350704 35.0 34.0 37.0 30.0 38.0 86-87 34.174897507588405 35.0 34.0 37.0 29.5 38.0 88-89 34.04692239314775 35.0 34.0 36.5 29.0 38.0 90-91 33.92773651308093 35.0 34.0 36.0 29.0 38.0 92-93 33.9215764108363 35.0 34.0 36.0 29.0 38.0 94-95 33.8977578942375 35.0 34.0 36.0 29.5 38.0 96-97 33.89739211939688 35.0 34.0 36.0 30.0 38.0 98-99 33.96662641499715 35.0 34.0 35.5 30.0 38.0 100 33.026731606133325 34.0 32.0 35.0 27.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 3.0 16 11.0 17 11.0 18 16.0 19 53.0 20 110.0 21 287.0 22 840.0 23 2168.0 24 4850.0 25 9845.0 26 17090.0 27 26642.0 28 37937.0 29 50505.0 30 65572.0 31 83515.0 32 106531.0 33 138330.0 34 186726.0 35 262529.0 36 418276.0 37 1043579.0 38 715244.0 39 392525.0 40 14691.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.8103036262195 20.200867216004088 13.603535719136941 24.385293438639465 2 15.062117226542071 21.135956883682148 40.44826927909246 23.35365661068332 3 18.36392618139039 25.637592071322103 30.41215027012433 25.58633147716318 4 12.748101246718024 17.59519759499636 38.57027944527952 31.0864217130061 5 13.24382610289987 37.79385368902196 35.7160625017119 13.246257706366274 6 30.637421091672568 37.59359577135773 18.522669531673174 13.246313605296537 7 27.096335657424525 32.31276793056011 22.934967743522293 17.655928668493072 8 24.832736425923017 36.511755824528784 21.08513239382138 17.570375355726817 9 25.608473830636303 16.556368760771026 21.209647260980365 36.6255101476123 10-11 24.4681775774857 26.571165207611426 28.841961426384184 20.118695788518696 12-13 25.045068512523876 24.015298419234153 29.016435962464985 21.923197105776985 14-15 22.141218404679748 25.603530690700243 26.403458916423634 25.85179198819637 16-17 21.893690822947864 28.663602185151493 27.228001645110346 22.214705346790296 18-19 21.899034709133332 28.093258112765728 28.631139487066687 21.376567691034253 20-21 22.59496984270242 27.22609042968734 28.548922350723544 21.630017376886702 22-23 22.361997274537895 27.162206925469096 28.3434781089486 22.132317691044413 24-25 22.20172089956927 27.272897565208932 28.47994846695337 22.045433068268423 26-27 22.20143232160168 27.43721496626722 28.31503355937456 22.046319152756546 28-29 22.08894730463604 27.57086275773142 28.295800811507434 22.044389126125115 30-31 22.323435461722706 27.41007834695427 28.228890588341677 22.037595602981348 32-33 22.092703251635225 27.528206258976283 28.21321992564019 22.165870563748307 34-35 22.25079292646341 27.535585681239045 28.24379777890228 21.969823613395263 36-37 22.240038855440293 27.51497730947378 28.15656072547197 22.088423109613952 38-39 22.130680833647666 27.603487785618725 28.22587089384817 22.03996048688543 40-41 22.323508791398698 27.452537544280663 28.13857355141759 22.08538011290305 42-43 22.273772461447678 27.512299960650626 27.970957887419956 22.24296969048174 44-45 22.33576240096811 27.601225844941723 27.824083972058233 22.238927782031933 46-47 22.338980908881727 27.570022425774976 27.64394045068535 22.447056214657945 48-49 22.33367919443503 27.652273338389616 27.656086652976917 22.35796081419843 50-51 22.27137369298136 27.750384248134047 27.595314175683228 22.38292788320136 52-53 22.413777985147814 27.68308443544815 27.487498186665952 22.41563939273808 54-55 22.378332867394548 27.609274041470155 27.535164133477398 22.477228957657896 56-57 22.40773079397955 27.694413513839596 27.54944463156413 22.348411060616723 58-59 22.365875791806896 27.784605647121026 27.558263896655856 22.291254664416225 60-61 22.49549103250376 27.732543533141474 27.48561853010551 22.286346904249257 62-63 22.447288134382212 27.78234080240382 27.404519115663096 22.365851947550873 64-65 22.345677674905527 27.698919266896528 27.530182954152636 22.425220104045305 66-67 22.429603922170976 27.70457517425183 27.38238261093376 22.483438292643438 68-69 22.34443238640478 27.70225121304271 27.44489915519235 22.50841724536016 70-71 22.356880988711417 27.53368183856491 27.494637825112413 22.614799347611267 72-73 22.42220585052333 27.56268798826193 27.4692637793826 22.54584238183214 74-75 22.29468444365254 27.678467027291887 27.476850756282868 22.549997772772702 76-77 22.403893521490392 27.662323668780946 27.347928463602727 22.585854346125934 78-79 22.306487877170298 27.64707402558863 27.397336693582524 22.64910140365855 80-81 22.334474430387242 27.66998476651681 27.409690430635656 22.58585037246029 82-83 22.376637132925246 27.662572127482765 27.545145513173143 22.41564522641885 84-85 22.45416805186555 27.64159356366374 27.41534389819506 22.48889448627565 86-87 22.317018334574456 27.689568870258714 27.51561786855317 22.477794926613658 88-89 22.35897619394744 27.617213566241 27.44951410742434 22.574296132387218 90-91 22.39461837478706 27.738714443213013 27.449148685562 22.417518496437918 92-93 22.447412248335695 27.660569100501124 27.483850920331076 22.40816773083211 94-95 22.287823630986974 27.719816274278198 27.55325833959659 22.439101755138235 96-97 22.293544429746987 27.8782066021232 27.62407209625688 22.20417687187293 98-99 23.43719393054036 29.727438212350403 27.829555422973705 19.00581243413553 100 29.94525816488388 41.257448041648026 28.797293793468093 0.0 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 503.0 1 446.5 2 593.5 3 840.0 4 1101.0 5 1809.5 6 2497.0 7 2973.5 8 3243.0 9 3120.5 10 2807.5 11 2533.0 12 2690.5 13 3546.5 14 5598.5 15 8435.5 16 10861.5 17 12512.0 18 13268.5 19 13348.5 20 13104.0 21 12922.5 22 13630.5 23 15310.5 24 18260.5 25 22763.5 26 28698.5 27 35784.0 28 43350.0 29 52094.5 30 61035.0 31 69654.5 32 78777.0 33 87624.0 34 96080.5 35 104667.0 36 113260.0 37 121027.5 38 127763.0 39 133138.5 40 138014.5 41 142629.5 42 145968.5 43 149224.0 44 153044.5 45 156361.0 46 157991.0 47 159058.5 48 158845.0 49 156478.0 50 153507.0 51 149441.0 52 143504.5 53 136350.5 54 128656.5 55 120010.5 56 110352.0 57 99978.5 58 89125.5 59 76641.0 60 63730.5 61 52176.0 62 41981.5 63 32826.0 64 24874.0 65 18990.5 66 14485.0 67 11252.5 68 8583.0 69 6155.5 70 4491.0 71 3256.5 72 2337.5 73 1688.5 74 1188.0 75 819.5 76 561.5 77 354.0 78 224.5 79 156.0 80 108.0 81 82.5 82 63.5 83 41.5 84 25.0 85 18.0 86 14.5 87 11.5 88 7.0 89 3.5 90 3.0 91 3.5 92 3.5 93 1.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0060091350031834445 3 0.0024875023966666352 4 3.3539358157302945E-4 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 6.987366282771447E-5 16-17 2.9346938387640075E-4 18-19 0.001467346919382004 20-21 2.795059006490686E-5 22-23 0.0011740314310576597 24-25 1.6775052142453743E-4 26-27 0.0020136897340419283 28-29 0.004701898251481588 30-31 0.00229911516309909 32-33 0.0017296605281061398 34-35 0.0019201554196386721 36-37 0.0018461673919974325 38-39 0.0 40-41 5.201813005226087E-4 42-43 0.005191864173832946 44-45 0.0032120007420016394 46-47 5.812068984788027E-4 48-49 0.013081122854650643 50-51 0.009457755359394703 52-53 0.012612906664336869 54-55 0.010985520865963593 56-57 0.008812351114856665 58-59 0.014375646606259299 60-61 0.00709947325763346 62-63 0.016511849809383115 64-65 0.012128503048948112 66-67 0.025051114899121533 68-69 0.017437151918414287 70-71 0.01792030293813058 72-73 0.015103879598340019 74-75 0.007829482909630717 76-77 0.009559291992710996 78-79 0.009480992303465892 80-81 0.008321938054814675 82-83 0.0033648606143065096 84-85 0.002740998090005858 86-87 0.011033758045448575 88-89 9.706718171909024E-4 90-91 2.3189942830993438E-4 92-93 0.0011392172595343156 94-95 0.0024799077474317952 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 18-19 93.0 20-21 273.0 22-23 533.0 24-25 1111.0 26-27 1874.0 28-29 4995.0 30-31 10349.0 32-33 13275.0 34-35 18092.0 36-37 27753.0 38-39 31530.0 40-41 31491.0 42-43 33833.0 44-45 37884.0 46-47 37906.0 48-49 35688.0 50-51 32690.0 52-53 32116.0 54-55 34410.0 56-57 35592.0 58-59 34883.0 60-61 33743.0 62-63 32874.0 64-65 34215.0 66-67 35249.0 68-69 35831.0 70-71 34966.0 72-73 32625.0 74-75 33637.0 76-77 33915.0 78-79 35657.0 80-81 35971.0 82-83 34458.0 84-85 34912.0 86-87 36715.0 88-89 39084.0 90-91 41299.0 92-93 44032.0 94-95 53610.0 96-97 125698.0 98-99 759456.0 100-101 1573568.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.07046842475634 #Duplication Level Percentage of deduplicated Percentage of total 1 92.04741958677245 81.98706779883913 2 5.939581473406361 10.58082608166618 3 1.2261932256446881 3.276528149623059 4 0.39096264158088695 1.3929290248875887 5 0.15581122927545984 0.6939089588701158 6 0.08312321268559861 0.444229409452615 7 0.04656402036476928 0.29032353739409256 8 0.030859726889394645 0.2198952263598744 9 0.01944856142585444 0.1559063228749652 >10 0.05854871554570574 0.8183051901518865 >50 7.48956393146214E-4 0.04520251520437168 >100 7.386500156667019E-4 0.09487778467612308 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 2.7949465131085787E-5 5 0.0 0.0 0.0 0.0 2.7949465131085787E-5 6 5.5898930262171574E-5 0.0 0.0 2.7949465131085787E-5 5.5898930262171574E-5 7 5.5898930262171574E-5 0.0 0.0 2.7949465131085787E-5 8.384839539325736E-5 8 5.5898930262171574E-5 0.0 0.0 2.7949465131085787E-5 1.1179786052434315E-4 9 5.5898930262171574E-5 2.7949465131085787E-5 0.0 2.7949465131085787E-5 1.1179786052434315E-4 10-11 5.5898930262171574E-5 2.7949465131085787E-5 0.0 2.7949465131085787E-5 1.1179786052434315E-4 12-13 5.5898930262171574E-5 2.7949465131085787E-5 0.0 5.5898930262171574E-5 1.1179786052434315E-4 14-15 8.384839539325736E-5 5.5898930262171574E-5 0.0 8.384839539325736E-5 1.3974732565542894E-4 16-17 1.6769679078651472E-4 5.5898930262171574E-5 0.0 1.5372205822097184E-4 1.6769679078651472E-4 18-19 1.6769679078651472E-4 5.5898930262171574E-5 0.0 1.956462559176005E-4 1.956462559176005E-4 20-21 1.816715233520576E-4 5.5898930262171574E-5 0.0 2.0962098848314342E-4 2.235957210486863E-4 22-23 2.235957210486863E-4 6.987366282771447E-5 0.0 2.6551991874531494E-4 2.235957210486863E-4 24-25 2.794946513108578E-4 9.782312795880025E-5 0.0 3.2141884900748657E-4 2.5154518617977206E-4 26-27 3.2141884900748657E-4 1.1179786052434315E-4 0.0 3.633430467041152E-4 2.5154518617977206E-4 28-29 3.3539358157302945E-4 1.1179786052434315E-4 0.0 9.363070818913738E-4 2.5154518617977206E-4 30-31 3.3539358157302945E-4 1.6769679078651472E-4 0.0 0.00265519918745315 2.5154518617977206E-4 32-33 3.3539358157302945E-4 1.6769679078651472E-4 0.0 0.005645791956479329 2.5154518617977206E-4 34-35 4.052672444007439E-4 2.096209884831434E-4 0.0 0.010103731644887513 3.3539358157302945E-4 36-37 4.7514090722845835E-4 2.5154518617977206E-4 0.0 0.016392361299381812 3.3539358157302945E-4 38-39 4.7514090722845835E-4 2.5154518617977206E-4 0.0 0.025252341745936005 3.3539358157302945E-4 40-41 4.7514090722845835E-4 2.7949465131085787E-4 0.0 0.0346014378322842 3.3539358157302945E-4 42-43 4.7514090722845835E-4 2.7949465131085787E-4 0.0 0.043740912930149256 3.493683141385723E-4 44-45 4.7514090722845835E-4 3.633430467041152E-4 0.0 0.05321578160958734 3.91292511835201E-4 46-47 4.7514090722845835E-4 4.471914420973726E-4 0.0 0.06372478049887559 3.91292511835201E-4 48-49 4.7514090722845835E-4 4.471914420973726E-4 0.0 0.07477879395822001 3.91292511835201E-4 50-51 4.7514090722845835E-4 4.471914420973726E-4 0.0 0.08530176758007382 4.471914420973726E-4 52-53 5.030903723595441E-4 4.471914420973726E-4 0.0 0.09553127181805121 4.471914420973726E-4 54-55 5.030903723595441E-4 4.471914420973726E-4 0.0 0.10426547967151553 4.471914420973726E-4 56-57 5.030903723595441E-4 4.471914420973726E-4 0.0 0.11266429394340681 4.611661746629155E-4 58-59 5.030903723595441E-4 4.7514090722845835E-4 0.0 0.12095131035477374 4.7514090722845835E-4 60-61 5.450145700561729E-4 4.7514090722845835E-4 0.0 0.1282740702191182 4.7514090722845835E-4 62-63 6.148882328838873E-4 5.030903723595441E-4 0.0 0.13495399238544772 4.7514090722845835E-4 64-65 6.148882328838873E-4 5.589893026217157E-4 0.0 0.14173173767973601 4.891156397940012E-4 66-67 6.428376980149731E-4 5.589893026217157E-4 0.0 0.14848153350889326 5.17065104925087E-4 68-69 7.546355585393163E-4 5.589893026217157E-4 0.0 0.1559021165011965 5.729640351872586E-4 70-71 7.546355585393163E-4 5.589893026217157E-4 0.0 0.16245626607443614 6.148882328838873E-4 72-73 8.943828841947452E-4 5.589893026217157E-4 0.0 0.1674312708677694 6.428376980149731E-4 74-75 8.943828841947452E-4 5.729640351872586E-4 0.0 0.17074328248580306 6.56812430580516E-4 76-77 9.363070818913738E-4 6.009135003183444E-4 0.0 0.17427888982488543 6.707871631460589E-4 78-79 9.782312795880025E-4 6.148882328838873E-4 0.0 0.17631920077945468 6.707871631460589E-4 80-81 9.782312795880025E-4 6.148882328838873E-4 0.0 0.1771437100008217 6.707871631460589E-4 82-83 9.782312795880025E-4 6.148882328838873E-4 0.0 0.1777585982337056 6.707871631460589E-4 84-85 9.782312795880025E-4 6.428376980149731E-4 0.0 0.1781778402106719 6.707871631460589E-4 86-87 9.782312795880025E-4 6.707871631460589E-4 0.0 0.1783455370014584 6.847618957116018E-4 88 9.782312795880025E-4 6.707871631460589E-4 0.0 0.1783734864665895 6.987366282771447E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3805 0.0 40.814667 1 GTATCAA 6640 0.0 30.311527 1 TCAACGC 7180 0.0 26.991728 4 ATCAACG 7205 0.0 26.840597 3 CAACGCA 7310 0.0 26.511711 5 AACGCAG 7535 0.0 25.82997 6 TATCAAC 7835 0.0 25.424107 2 GTACATG 9690 0.0 23.335035 1 TACATGG 9715 0.0 22.678194 2 ACGCAGA 9040 0.0 21.392311 7 ACATGGG 9875 0.0 21.344696 3 CGCAGAG 9250 0.0 20.861881 8 TGCGCGA 240 0.005060831 19.586088 94 TATACCG 220 2.4003748E-7 18.822903 5 CATGGGG 7415 0.0 18.37359 4 GCAGAGT 11090 0.0 17.213875 9 CTAGTAC 1050 0.0 16.564154 3 ACGGGCG 330 3.7216523E-9 16.313183 5 AGAGTAC 9885 0.0 16.08681 10-11 GAGTACT 6130 0.0 16.044777 12-13 >>END_MODULE