FastQCFastQC Report
Fri 27 May 2016
SRR2140306_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140306_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8787200
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA90750.043.0921441
GTATCAA155150.033.04411
ATCAACG175800.028.019223
TCAACGC176250.027.9702764
CAACGCA178500.027.5488955
AACGCAG185650.026.5320036
TATCAAC193900.025.8913122
ACGCAGA218800.022.5121887
CGCAGAG223250.022.0635838
GTACATG220100.020.669761
TACATGG220750.019.9967542
ACATGGG223450.019.2589073
TATACCG6000.018.4251865
GCAGAGT267850.018.3133169
GAGTACT149700.017.32757612-13
AGAGTAC239300.016.71682210-11
CATGGGG164300.016.672014
CAGAGTA261000.016.62119710-11
GTACTTT170750.015.13200514-15
GTGGTAT53450.014.7093991
Produced by FastQC (version 0.11.5)