##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140306_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8787200
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.00145279497451	31.0	31.0	34.0	30.0	34.0
2	32.1109105289512	33.0	31.0	34.0	30.0	34.0
3	31.84724371813547	31.0	31.0	34.0	30.0	34.0
4	35.90574141933722	37.0	35.0	37.0	35.0	37.0
5	35.84401231336489	37.0	35.0	37.0	35.0	37.0
6	35.8853700837582	37.0	35.0	37.0	35.0	37.0
7	35.87131862254188	37.0	35.0	37.0	35.0	37.0
8	35.86695204388201	37.0	35.0	37.0	35.0	37.0
9	37.64399899854334	39.0	37.0	39.0	35.0	39.0
10-11	37.55539398215586	39.0	37.0	39.0	35.0	39.0
12-13	37.51041537691187	39.0	37.0	39.0	35.0	39.0
14-15	38.76680643435907	40.0	38.0	41.0	34.5	41.0
16-17	38.707517525491625	40.0	38.0	41.0	34.0	41.0
18-19	38.709256759832485	40.0	38.0	41.0	34.0	41.0
20-21	38.771947095979485	40.0	38.0	41.0	34.0	41.0
22-23	38.73145966668719	40.0	38.0	41.0	34.0	41.0
24-25	38.64543349163908	40.0	38.0	41.0	34.0	41.0
26-27	38.499457134271246	40.0	38.0	41.0	34.0	41.0
28-29	38.37530346265453	40.0	38.0	41.0	34.0	41.0
30-31	38.258185920951526	40.0	38.0	41.0	34.0	41.0
32-33	38.07439294905775	40.0	37.5	41.0	33.0	41.0
34-35	38.16140148726308	40.0	38.0	41.0	33.0	41.0
36-37	38.12137473891376	40.0	38.0	41.0	33.0	41.0
38-39	38.08821155664887	40.0	37.5	41.0	33.0	41.0
40-41	38.02906613392592	40.0	37.0	41.0	33.0	41.0
42-43	38.061425639863586	40.0	37.0	41.0	33.0	41.0
44-45	38.09109643408769	40.0	37.0	41.0	33.0	41.0
46-47	38.04028317061724	40.0	37.0	41.0	33.0	41.0
48-49	37.95560023079502	40.0	37.0	41.0	33.0	41.0
50-51	37.86283073772589	40.0	37.0	41.0	33.0	41.0
52-53	37.600548066081814	40.0	36.5	41.0	32.0	41.0
54-55	37.54925577096875	39.5	36.0	41.0	32.0	41.0
56-57	37.39031767874194	39.0	36.0	41.0	31.5	41.0
58-59	37.22303725837263	39.0	35.5	41.0	31.0	41.0
60-61	37.07952096719457	39.0	35.0	41.0	31.0	41.0
62-63	36.84333638118534	38.5	35.0	40.0	31.0	41.0
64-65	36.49298810009215	38.0	35.0	40.0	31.0	41.0
66-67	36.24989878737753	37.5	35.0	40.0	30.5	41.0
68-69	35.963692213560634	37.0	34.5	40.0	30.0	41.0
70-71	35.69503441493278	36.5	34.0	39.0	30.0	41.0
72-73	35.39862505005883	36.0	34.0	39.0	30.0	40.5
74-75	35.07454425756737	36.0	34.0	39.0	29.5	40.0
76-77	33.829211255563536	34.5	32.5	37.0	28.5	39.0
78-79	34.43683237979309	35.0	33.5	37.0	29.0	39.0
80-81	34.35896378234255	35.0	34.0	37.0	30.0	39.0
82-83	34.0813979309287	35.0	34.0	36.0	29.0	38.5
84-85	33.83897862268911	35.0	33.0	36.0	29.0	37.0
86-87	33.62971683015763	35.0	33.0	35.5	29.0	37.0
88-89	33.45418912347007	35.0	33.0	35.0	29.0	36.5
90-91	33.31749915988701	35.0	33.0	35.0	29.0	36.0
92-93	33.28488958366893	35.0	33.0	35.0	29.0	36.0
94-95	33.2727394503749	35.0	33.0	35.0	29.0	36.0
96-97	33.25439345385769	35.0	33.0	35.0	29.0	35.5
98-99	33.35598522019735	35.0	33.0	35.0	29.5	35.0
100	32.27656090587331	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	5.0
19	5.0
20	50.0
21	305.0
22	1224.0
23	4333.0
24	11267.0
25	24541.0
26	44705.0
27	71547.0
28	102924.0
29	139487.0
30	178746.0
31	223628.0
32	279062.0
33	354100.0
34	472913.0
35	649632.0
36	994042.0
37	1694380.0
38	2216030.0
39	1274052.0
40	50222.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.447321103423164	20.503004369992716	13.821342407137655	24.228332119446467
2	14.958016934806606	21.43337993123329	40.55742666692009	23.05117646704002
3	18.232174917696074	25.864464368954664	30.669946229314764	25.233414484034494
4	12.603947028212048	17.888072698735247	38.82964340729573	30.67833686575698
5	13.096663328477787	37.90140203932994	35.90383739985433	13.098097232337945
6	30.257419883466863	38.0289853423161	18.631224963583396	13.082369810633649
7	26.728286598689	32.522692097596504	23.09334031318281	17.655680990531682
8	24.60428805535324	36.7235182993445	21.23996267297888	17.432230972323378
9	25.45150901310998	16.626320101966495	21.38639156955572	36.535779315367805
10-11	24.268225373270212	26.764418699927166	29.03026561361981	19.93709031318281
12-13	24.819601238164605	24.254222050254917	29.183397441733433	21.742779269847052
14-15	21.89179486797229	25.812542224109315	26.633293842132343	25.662369065786052
16-17	21.651357813159873	29.002321677452642	27.353715600031453	21.992604909356025
18-19	21.666384812562846	28.28726858564169	28.873380970090995	21.172965631704464
20-21	22.381787505725903	27.461386792157576	28.76030306737644	21.39652263474008
22-23	22.137063947904945	27.470905673745747	28.578651213810822	21.813379164538485
24-25	21.93753876042351	27.558594945142705	28.675430210461094	21.828436083972687
26-27	21.942086595333336	27.764173238502917	28.518414135109033	21.77532603105472
28-29	21.837577126322277	27.81812374386703	28.541216322584123	21.80308280722657
30-31	22.118588430082823	27.665893639291355	28.50461920406506	21.710898726560753
32-33	21.835903821793476	27.78850696964496	28.472391472589464	21.903197735972103
34-35	21.9689506579487	27.85907007384601	28.516048894525888	21.655930373679404
36-37	22.0121347649163	27.790183795196327	28.412290055429118	21.78539138445825
38-39	21.88373458135702	27.812850453592556	28.5015725862944	21.801842378756024
40-41	22.07769455144107	27.711336080304182	28.375396262990236	21.83557310526451
42-43	21.998378699493728	27.776268401683225	28.256289755048137	21.96906314377491
44-45	22.039964004303513	27.919980801973555	28.109542616910776	21.93051257681216
46-47	22.10247185378774	27.833505071277354	27.869574925380718	22.194448149554187
48-49	22.07133444727712	27.93944266206843	27.904025976852527	22.085196913801923
50-51	22.039608860640673	28.041462107897715	27.810991293415118	22.107937738046495
52-53	22.111562612545665	28.00960819552755	27.72996866532702	22.14886052659977
54-55	22.13934104661873	27.939348319002537	27.73308229763106	22.188228336747667
56-57	22.17410148258792	28.01716983624501	27.696531821620802	22.112196859546273
58-59	22.080314673802174	28.14156944095491	27.749961501517394	22.028154383725518
60-61	22.22105513848133	28.085865073522303	27.680020165087747	22.01305962290862
62-63	22.136049585154208	28.182233952053252	27.597720701882295	22.083995760910245
64-65	22.101430164285908	28.133949713398998	27.63471494546289	22.1299051768522
66-67	22.179558471374683	28.110196705875374	27.556550544655146	22.1536942780948
68-69	22.114598058592197	28.05802608007796	27.671167463844625	22.156208397485212
70-71	22.07167864810231	27.883812837363198	27.73718204806807	22.307326466466424
72-73	22.171663942574956	27.942295539180005	27.673356442269387	22.212684075975652
74-75	22.04367444144103	28.03897204897572	27.655228858481735	22.262124651101516
76-77	22.134057125862235	27.984654884484094	27.553313977486155	22.327974012167516
78-79	22.119822566659224	28.017761119800998	27.56693749629927	22.295478817240504
80-81	22.073671276882237	28.017368858923557	27.640239078790447	22.26872078540376
82-83	22.09074843535407	28.000705939531677	27.749123538427412	22.159422086686845
84-85	22.231623343174764	28.016779236741442	27.60995390446083	22.141643515622967
86-87	22.072133656101446	27.998082519096418	27.711550039509824	22.21823378529231
88-89	22.172320103778574	27.9369462690006	27.686257388089047	22.20447623913178
90-91	22.121043126461853	28.066439119614234	27.656349280812776	22.156168473111137
92-93	22.175214247146833	28.0293415909437	27.67621040820188	22.119233753707583
94-95	22.002903826757958	28.07879047694169	27.759278715400594	22.159026980899764
96-97	22.020304244546896	28.221090701280747	27.839502911052243	21.91910214312011
98-99	23.106518507496496	30.171140616788605	28.06182955313889	18.660511322576014
100	29.41561116246113	41.636582463055454	28.947806374483413	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1542.0
1	1387.5
2	1909.5
3	2741.0
4	3571.5
5	5705.5
6	7698.5
7	9006.0
8	9719.0
9	9529.5
10	8812.0
11	8086.0
12	8634.0
13	11219.5
14	17387.5
15	25941.5
16	33373.0
17	38399.0
18	40845.0
19	40918.5
20	39601.0
21	38348.0
22	38648.0
23	42356.5
24	50425.5
25	62739.0
26	78739.5
27	96860.0
28	117165.5
29	140676.5
30	163997.5
31	186074.5
32	208366.0
33	229933.0
34	250650.5
35	270443.5
36	291069.0
37	310043.0
38	324493.0
39	335225.5
40	346091.5
41	357729.5
42	365324.5
43	371783.0
44	379854.5
45	386250.0
46	389406.0
47	391197.0
48	389377.5
49	382164.0
50	374364.0
51	362545.5
52	344138.5
53	324326.5
54	303674.0
55	281124.5
56	257762.0
57	232875.5
58	206036.5
59	176881.0
60	147290.5
61	120511.5
62	96547.0
63	75078.0
64	57406.5
65	44575.0
66	34379.0
67	26684.5
68	20520.5
69	14916.5
70	10814.0
71	8014.0
72	5801.0
73	3981.5
74	2689.0
75	1858.0
76	1273.5
77	846.0
78	570.5
79	370.5
80	234.0
81	147.5
82	112.5
83	90.0
84	70.5
85	54.0
86	39.5
87	27.5
88	19.0
89	12.5
90	8.5
91	7.0
92	5.0
93	3.0
94	1.0
95	0.0
96	0.0
97	0.0
98	1.0
99	2.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.00774990895848507
3	0.0029133284777858705
4	3.641660597232338E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	1.1949198834668608E-4
16-17	2.1622359796067005E-4
18-19	0.0016842680262199564
20-21	6.25936735663673E-5
22-23	0.0016560421980039172
24-25	2.4476462693487865E-4
26-27	0.0024430056463612087
28-29	0.005494166665431809
30-31	0.0026269448243361995
32-33	0.001999923899743872
34-35	0.0021757032313164506
36-37	0.002815169523600331
38-39	0.0
40-41	6.670130412264823E-4
42-43	0.006094115564943594
44-45	0.003944148683549915
46-47	4.641544510579668E-4
48-49	0.015863267781233297
50-51	0.010555278298854321
52-53	0.014986457829649927
54-55	0.012664357902677541
56-57	0.011230024521149686
58-59	0.017431717756409674
60-61	0.008946596237330484
62-63	0.019918279446559918
64-65	0.014269368668221857
66-67	0.03077077250219987
68-69	0.02124228350584316
70-71	0.021710536696865117
72-73	0.01938251875153466
74-75	0.009641040588203136
76-77	0.01169464898500101
78-79	0.012611451479515172
80-81	0.010278153274313091
82-83	0.004594796830733171
84-85	0.003098127510275199
86-87	0.013661948526198685
88-89	0.001210119689594746
90-91	1.7000023638128107E-4
92-93	0.0011207510350465441
94-95	0.002581523807073224
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	229.0
20-21	692.0
22-23	1474.0
24-25	3570.0
26-27	5632.0
28-29	13540.0
30-31	28574.0
32-33	35758.0
34-35	48961.0
36-37	72875.0
38-39	84764.0
40-41	84415.0
42-43	90799.0
44-45	103246.0
46-47	100899.0
48-49	93677.0
50-51	86961.0
52-53	83866.0
54-55	90370.0
56-57	92850.0
58-59	91107.0
60-61	88586.0
62-63	86335.0
64-65	90060.0
66-67	93655.0
68-69	93302.0
70-71	90640.0
72-73	85568.0
74-75	86990.0
76-77	87075.0
78-79	92122.0
80-81	94007.0
82-83	90615.0
84-85	91135.0
86-87	96897.0
88-89	102781.0
90-91	107423.0
92-93	116688.0
94-95	143157.0
96-97	316976.0
98-99	1787458.0
100-101	3731471.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.26970746129419
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.01163275224528	76.75265592897401
2	6.883202577004679	11.738573402760306
3	1.7205612928554765	4.401352743330378
4	0.6276839798213305	2.140897173500229
5	0.2955607730328392	1.2601190326772083
6	0.1544642112359741	0.7902670863198636
7	0.09071515219650643	0.5414678143072017
8	0.050451216919815696	0.3441568406255183
9	0.039266521678397476	0.3013420334885567
>10	0.1246838893568029	1.5923606817101956
>50	0.0015753099992319938	0.08758350667694263
>100	1.8083499307201066E-4	0.0350415074551404
>500	2.1488660628023397E-5	0.01418224817446617
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1380189366351057E-5
2	0.0	2.2760378732702113E-5	0.0	1.1380189366351057E-5	1.1380189366351057E-5
3	0.0	2.2760378732702113E-5	0.0	2.2760378732702113E-5	1.1380189366351057E-5
4	0.0	5.690094683175528E-5	0.0	2.2760378732702113E-5	1.1380189366351057E-5
5	0.0	6.828113619810633E-5	0.0	2.2760378732702113E-5	1.1380189366351057E-5
6	0.0	6.828113619810633E-5	0.0	3.414056809905317E-5	2.2760378732702113E-5
7	0.0	6.828113619810633E-5	0.0	3.414056809905317E-5	2.2760378732702113E-5
8	0.0	6.828113619810633E-5	0.0	6.828113619810633E-5	2.2760378732702113E-5
9	0.0	1.9346321922796795E-4	0.0	1.1380189366351056E-4	3.414056809905317E-5
10-11	0.0	1.9346321922796795E-4	0.0	1.5932265112891477E-4	3.414056809905317E-5
12-13	0.0	1.9915331391114348E-4	0.0	2.0484340859431901E-4	3.414056809905317E-5
14-15	0.0	2.446740713765477E-4	0.0	2.4467407137654775E-4	3.414056809905317E-5
16-17	0.0	2.6174435542607427E-4	0.0	3.1295520757465404E-4	3.414056809905317E-5
18-19	0.0	3.0726511289147853E-4	0.0	3.755462490895848E-4	4.552075746540423E-5
20-21	0.0	3.8123634377276033E-4	0.0	3.9830662782228696E-4	6.259104151493081E-5
22-23	0.0	4.267571012381646E-4	0.0	4.6089766933721775E-4	8.535142024763292E-5
24-25	0.0	4.893481427530954E-4	0.0	5.462490895848507E-4	1.0811179898033503E-4
26-27	0.0	5.234887108521485E-4	0.0	6.316005098324837E-4	1.194919883466861E-4
28-29	0.0	5.348689002184995E-4	0.0	0.0010299071376547707	1.2518208302986163E-4
30-31	0.0	6.600509832483612E-4	0.0	0.0027141751638747273	1.422523670793882E-4
32-33	0.0	6.600509832483612E-4	0.0	0.005826656955571741	1.7070284049526584E-4
34-35	0.0	8.421340131099782E-4	0.0	0.010008876547705754	1.9346321922796795E-4
36-37	0.0	0.001024217042971595	0.0	0.016148488710852147	1.9346321922796795E-4
38-39	0.0	0.0010811179898033503	0.0	0.025246950109249818	2.1053350327749455E-4
40-41	0.0	0.0011778495994173343	0.0	0.03543790968681719	2.1622359796067005E-4
42-43	0.0	0.0012461307356154408	0.0	0.044604652221412966	2.1622359796067005E-4
44-45	0.0	0.0013428623452294247	0.0	0.05454524763292061	2.1622359796067005E-4
46-47	0.0	0.001411143481427531	0.0	0.06550436999271668	2.219136926438456E-4
48-49	0.0	0.0014168335761107066	0.0	0.07606518572469045	2.2760378732702112E-4
50-51	0.0	0.001519255280407866	0.0	0.08630166605972323	2.2760378732702112E-4
52-53	0.0	0.0015306354697742172	0.0	0.09674867989803351	2.5036416605972325E-4
54-55	0.0	0.0015477057538237436	0.0	0.10581869082301529	2.7312454479242533E-4
56-57	5.690094683175528E-6	0.0015590859431900946	0.0	0.11385310451565914	2.7312454479242533E-4
58-59	1.1380189366351057E-5	0.0015590859431900946	0.0	0.12203546067006554	2.788146394756009E-4
60-61	1.1380189366351057E-5	0.0015761562272396212	0.0	0.1300698743627094	2.845047341587764E-4
62-63	1.1380189366351057E-5	0.0016046067006554989	0.0	0.13735888565185725	2.845047341587764E-4
64-65	1.1380189366351057E-5	0.0016615076474872541	0.0	0.1443690823015295	2.845047341587764E-4
66-67	1.1380189366351057E-5	0.0016728878368536051	0.0	0.15195966860888566	3.0726511289147853E-4
68-69	1.1380189366351057E-5	0.0017127184996358338	0.0	0.1589755553532411	3.1864530225782955E-4
70-71	1.1380189366351057E-5	0.0017297887836853604	0.0	0.1648989439184268	3.300254916241806E-4
72-73	1.1380189366351057E-5	0.0017297887836853604	0.0	0.16979811544064094	3.414056809905317E-4
74-75	1.1380189366351057E-5	0.0017809996358339403	0.0	0.1736161689730517	3.414056809905317E-4
76-77	1.1380189366351057E-5	0.001866351056081573	0.0	0.17740008193736345	3.414056809905317E-4
78-79	1.1380189366351057E-5	0.0018891114348142753	0.0	0.17963059905316825	3.414056809905317E-4
80-81	1.7070284049526587E-5	0.0018948015294974509	0.0	0.18039307174071378	3.584759650400583E-4
82-83	2.2760378732702113E-5	0.001917561908230153	0.0	0.18096208120903132	3.755462490895848E-4
84-85	2.2760378732702113E-5	0.0019346321922796795	0.0	0.18121813546977422	3.755462490895848E-4
86-87	2.2760378732702113E-5	0.0019346321922796795	0.0	0.18130348689002185	3.8123634377276033E-4
88	2.2760378732702113E-5	0.0019346321922796795	0.0	0.1813205571740714	3.869264384559359E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9075	0.0	43.092144	1
GTATCAA	15515	0.0	33.0441	1
ATCAACG	17580	0.0	28.01922	3
TCAACGC	17625	0.0	27.970276	4
CAACGCA	17850	0.0	27.548895	5
AACGCAG	18565	0.0	26.532003	6
TATCAAC	19390	0.0	25.891312	2
ACGCAGA	21880	0.0	22.512188	7
CGCAGAG	22325	0.0	22.063583	8
GTACATG	22010	0.0	20.66976	1
TACATGG	22075	0.0	19.996754	2
ACATGGG	22345	0.0	19.258907	3
TATACCG	600	0.0	18.425186	5
GCAGAGT	26785	0.0	18.313316	9
GAGTACT	14970	0.0	17.327576	12-13
AGAGTAC	23930	0.0	16.716822	10-11
CATGGGG	16430	0.0	16.67201	4
CAGAGTA	26100	0.0	16.621197	10-11
GTACTTT	17075	0.0	15.132005	14-15
GTGGTAT	5345	0.0	14.709399	1
>>END_MODULE