FastQCFastQC Report
Fri 27 May 2016
SRR2140305_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140305_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3586247
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50750.030.105761
GTATCAA77350.027.2064781
ATCAACG80500.025.3215793
CAACGCA80200.025.3121955
TCAACGC80350.025.2649424
AACGCAG83450.024.4250876
TATCAAC96500.021.6794452
GTACATG96150.021.3300461
ACGCAGA97950.020.7672867
CGCAGAG98500.020.6931348
TACATGG97250.020.3688832
ACATGGG99050.019.2905673
CATGGGG73950.018.3195574
AGAGTAC96750.017.42875110-11
GCAGAGT117050.017.1322659
GAGTACT55850.016.84714512-13
GGACCGA5200.016.629276
CTCGATA4103.1299167E-416.35385594
CAGAGTA107850.015.74951310-11
GTACTAG9050.015.4718461
Produced by FastQC (version 0.11.5)