FastQCFastQC Report
Fri 27 May 2016
SRR2140304_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140304_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8388913
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91800.043.644271
GTATCAA159350.033.9574131
ATCAACG183100.028.7126143
TCAACGC183900.028.6322544
CAACGCA186000.028.2641035
TATCAAC193700.027.4007842
AACGCAG193850.027.2477686
CGCAGAG228300.023.0816978
ACGCAGA228300.023.0635537
GTACATG234200.021.600921
TACATGG234400.021.034532
ACATGGG240200.020.0071143
GCAGAGT279350.018.6559989
CATGGGG186850.018.5576954
ACCGAAC13000.018.1642748
GAGTACT150250.017.57803712-13
CAGAGTA267000.017.06742510-11
AGAGTAC248300.016.38405210-11
GTGGTAT53450.016.1234931
ACGGGCG8750.016.0974625
Produced by FastQC (version 0.11.5)