##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140304_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8388913
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.03075785861648	33.0	31.0	34.0	30.0	34.0
2	32.13933116245216	33.0	31.0	34.0	30.0	34.0
3	31.8748218034923	31.0	31.0	34.0	30.0	34.0
4	35.9264795093238	37.0	35.0	37.0	35.0	37.0
5	35.868320484429866	37.0	35.0	37.0	35.0	37.0
6	35.91123081142932	37.0	35.0	37.0	35.0	37.0
7	35.896671594996874	37.0	35.0	37.0	35.0	37.0
8	35.89410022490399	37.0	35.0	37.0	35.0	37.0
9	37.674366273675744	39.0	37.0	39.0	35.0	39.0
10-11	37.586470321005834	39.0	37.0	39.0	35.0	39.0
12-13	37.541027127114084	39.0	37.0	39.0	35.0	39.0
14-15	38.80430486047477	40.0	38.0	41.0	35.0	41.0
16-17	38.74563516155192	40.0	38.0	41.0	34.0	41.0
18-19	38.74741030214523	40.0	38.0	41.0	34.0	41.0
20-21	38.80870852326484	40.0	38.0	41.0	34.5	41.0
22-23	38.76851625458479	40.0	38.0	41.0	34.0	41.0
24-25	38.67859130294145	40.0	38.0	41.0	34.0	41.0
26-27	38.53394678688784	40.0	38.0	41.0	34.0	41.0
28-29	38.409560454640555	40.0	38.0	41.0	34.0	41.0
30-31	38.292004372019505	40.0	38.0	41.0	34.0	41.0
32-33	38.10635735225471	40.0	37.5	41.0	33.5	41.0
34-35	38.19497719800035	40.0	38.0	41.0	33.5	41.0
36-37	38.152673708184594	40.0	38.0	41.0	33.0	41.0
38-39	38.11845369579798	40.0	38.0	41.0	33.0	41.0
40-41	38.05664385552397	40.0	37.0	41.0	33.0	41.0
42-43	38.08302674215062	40.0	37.0	41.0	33.0	41.0
44-45	38.10896735636565	40.0	37.5	41.0	33.0	41.0
46-47	38.054049616296	40.0	37.0	41.0	33.0	41.0
48-49	37.963629988532915	40.0	37.0	41.0	33.0	41.0
50-51	37.870279396824266	40.0	37.0	41.0	33.0	41.0
52-53	37.60742621979776	40.0	36.5	41.0	32.0	41.0
54-55	37.55056888329008	39.5	36.0	41.0	32.0	41.0
56-57	37.387075596644806	39.0	36.0	41.0	32.0	41.0
58-59	37.2190602836807	39.0	35.5	41.0	31.0	41.0
60-61	37.06957943598759	39.0	35.0	41.0	31.0	41.0
62-63	36.824375621370095	38.5	35.0	40.0	31.0	41.0
64-65	36.46906547276898	38.0	35.0	40.0	31.0	41.0
66-67	36.22356221805754	37.5	35.0	40.0	30.5	41.0
68-69	35.93435377110464	37.0	34.0	40.0	30.0	41.0
70-71	35.66451661342769	36.5	34.0	39.0	30.0	41.0
72-73	35.36728484067659	36.0	34.0	39.0	30.0	40.5
74-75	35.0451219604209	35.5	34.0	38.5	30.0	40.0
76-77	33.806338193394	34.5	32.5	36.5	28.5	39.0
78-79	34.4142684845708	35.0	33.5	37.0	29.0	39.0
80-81	34.33985116893504	35.0	34.0	37.0	30.0	39.0
82-83	34.063996782232486	35.0	34.0	36.0	29.0	38.5
84-85	33.82637542765956	35.0	33.0	36.0	29.0	37.0
86-87	33.62337564715193	35.0	33.0	35.5	29.0	37.0
88-89	33.452274945459166	35.0	33.0	35.0	29.0	36.5
90-91	33.31439559071933	35.0	33.0	35.0	29.0	36.0
92-93	33.283808273362155	35.0	33.0	35.0	29.0	36.0
94-95	33.2747554819524	35.0	33.0	35.0	29.0	36.0
96-97	33.2621318289154	35.0	33.0	35.0	29.0	35.0
98-99	33.360238922006694	35.0	33.0	35.0	29.5	35.0
100	32.236178385779695	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	3.0
19	10.0
20	51.0
21	245.0
22	1203.0
23	4051.0
24	10632.0
25	22866.0
26	42048.0
27	67923.0
28	97761.0
29	132101.0
30	169614.0
31	212370.0
32	265725.0
33	336900.0
34	452305.0
35	623406.0
36	962121.0
37	1652776.0
38	2119492.0
39	1169036.0
40	46273.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.799575225061936	20.276619867198526	13.699259963716395	24.22454494402314
2	15.00496889197047	21.23972424080147	40.57611727348028	23.17918959374778
3	18.326434958684256	25.87193878043666	30.461820562945995	25.339805697933087
4	12.730287108785493	17.600462803360067	38.689259443100596	30.97999064475384
5	13.12451327126649	37.98566035909539	35.78326536465452	13.106561004983602
6	30.589195525093654	37.738655771015864	18.45516814872201	13.21698055516847
7	26.928697436723926	32.478355658236055	23.01043055280225	17.58251635223777
8	24.845483556689647	36.647787383180635	20.99473435950522	17.511994700624502
9	25.644323644791644	16.563719280435976	21.273066009863257	36.51889106490913
10-11	24.511626238107368	26.632806896435806	28.802748341769668	20.052818523687158
12-13	25.02841548124292	24.099528747049824	29.075411796498546	21.796643975208706
14-15	22.074607865797915	25.649092771508446	26.524314686750984	25.75198467594265
16-17	21.779378545873087	28.782415121810818	27.26623708656551	22.171969245750585
18-19	21.814997252572535	28.17551683613656	28.797527976881916	21.211957934408993
20-21	22.561865246802356	27.291733597819416	28.667697033889468	21.47870412148876
22-23	22.334517747706325	27.238371186725495	28.49136821290297	21.935742852665207
24-25	22.1214777400559	27.34868855253556	28.62360696661728	21.906226740791254
26-27	22.095772413011446	27.52027893603441	28.440207114181238	21.943741536772908
28-29	21.99099820041615	27.713143528826027	28.415652827471877	21.880205443285945
30-31	22.267738487410554	27.47914156754382	28.37570196820176	21.87741797684387
32-33	22.047262274384437	27.573550114659735	28.351445586987122	22.027742023968706
34-35	22.137593989307348	27.62815508873785	28.41421245460053	21.820038467354276
36-37	22.1635819067094	27.607929274114255	28.29068719144961	21.937801627726735
38-39	22.035771645949925	27.703538947676247	28.350359534889424	21.9103298714844
40-41	22.224365689953032	27.552179337483867	28.222818085739735	22.000636886823365
42-43	22.16572456291938	27.678860299756785	28.03866183635898	22.116753300964856
44-45	22.19504943830192	27.74337892376077	27.967143474369365	22.094428163567947
46-47	22.208814363275184	27.736386644833367	27.752986246823646	22.301812745067807
48-49	22.19217428264172	27.80254081965779	27.728757615210288	22.2765272824902
50-51	22.15136845843815	27.905628753575783	27.632453335640548	22.31054945234552
52-53	22.28372003556698	27.820298702874474	27.555374238154627	22.340607023403923
54-55	22.262568214973825	27.7576584574325	27.604838386802932	22.37493494079074
56-57	22.319428653537255	27.828208338473086	27.541403245792917	22.31095976219674
58-59	22.200389936765678	27.999716670395458	27.578104899741707	22.221788493097154
60-61	22.334351621173663	27.897347227579843	27.574027099814213	22.19427405143228
62-63	22.339529807990818	27.97014635463676	27.430737904727543	22.25958593264488
64-65	22.24459299808314	27.919772642857538	27.510419847998236	22.325214511061088
66-67	22.286811542540878	27.906294719936298	27.44026083517105	22.36663290235178
68-69	22.219295511523406	27.892051224871413	27.536688690542753	22.351964573062435
70-71	22.198924732352676	27.72808928015906	27.542833617407492	22.53015237008077
72-73	22.279448062025068	27.769861222504595	27.53328973292658	22.417400982543757
74-75	22.165489811756466	27.913361386651726	27.48900262614427	22.43214617544754
76-77	22.255021337520372	27.85883318279858	27.416150439166042	22.469995040515
78-79	22.19811820517166	27.783928878409075	27.47934652603447	22.538606390384796
80-81	22.204778469142273	27.886162671631805	27.499703377235562	22.409355481990364
82-83	22.216477057971876	27.873457127090322	27.609152594886716	22.300913220051086
84-85	22.389091250347157	27.843156434361287	27.47831034331086	22.289441971980697
86-87	22.195702884770576	27.902980191867027	27.517614441925573	22.38370248143682
88-89	22.27858924980633	27.812474929852655	27.5097645285005	22.399171291840517
90-91	22.27776117299683	27.898232415294487	27.520463718184118	22.303542693524562
92-93	22.313357530015665	27.86408085781098	27.538062044788912	22.284499567384444
94-95	22.092356359325834	27.971079119733982	27.630336274949524	22.30622824599066
96-97	22.140248036105614	28.09945698821823	27.69910520777809	22.061189767898064
98-99	23.215829609304052	30.050301757034877	27.908743730521508	18.825124903139567
100	22.246922300081163	31.04405329103755	21.56864081419387	25.140383594687414
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1493.0
1	1379.5
2	1981.5
3	2869.5
4	3744.5
5	5909.5
6	7875.5
7	9215.0
8	10078.0
9	9867.0
10	9058.0
11	8177.5
12	8548.5
13	11038.5
14	16974.0
15	25228.0
16	32324.0
17	36961.0
18	38877.0
19	38304.0
20	36498.5
21	34823.0
22	34634.0
23	37519.0
24	44124.0
25	54318.5
26	68140.0
27	84249.0
28	101834.0
29	122308.5
30	143250.0
31	163937.5
32	185085.5
33	204919.5
34	224434.0
35	243470.5
36	264691.0
37	285656.5
38	299747.0
39	309846.0
40	320238.5
41	329158.0
42	336364.5
43	344044.0
44	351894.5
45	361325.0
46	367729.0
47	370018.0
48	369920.5
49	365023.0
50	357310.5
51	346668.5
52	331779.0
53	314390.0
54	296759.0
55	275861.5
56	253983.5
57	230719.0
58	205285.0
59	178768.0
60	149097.0
61	121714.5
62	98153.0
63	77494.5
64	59693.0
65	45470.0
66	34744.5
67	26764.0
68	20717.5
69	15370.0
70	11164.5
71	8210.5
72	6022.5
73	4211.0
74	2867.0
75	1922.0
76	1250.0
77	837.5
78	563.5
79	359.5
80	235.5
81	159.5
82	120.0
83	86.0
84	58.5
85	40.5
86	28.0
87	20.0
88	13.5
89	12.0
90	11.5
91	9.0
92	7.0
93	3.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.008642359266331645
3	0.002646350009828449
4	4.410583349714081E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	1.4304594647721343E-4
16-17	2.80131645184543E-4
18-19	0.0018655575519736587
20-21	6.55651678727578E-5
22-23	0.0014783352061893127
24-25	2.2657320959017617E-4
26-27	0.0024576309201465607
28-29	0.005748547432202061
30-31	0.002660824890396437
32-33	0.002080917340326175
34-35	0.002173011569510878
36-37	0.002614656468941543
38-39	0.0
40-41	7.50720861246663E-4
42-43	0.006346113818731146
44-45	0.004027449136046291
46-47	5.964653211255073E-4
48-49	0.016137671858184578
50-51	0.011180269392611762
52-53	0.015066262733612229
54-55	0.01324666582016811
56-57	0.011678481374695045
58-59	0.01787741813556183
60-61	0.00922728941088853
62-63	0.020346718597135938
64-65	0.015404392216663372
66-67	0.03194239925834206
68-69	0.022624554294848005
70-71	0.022429319362615743
72-73	0.020855998907647787
74-75	0.010438103162633819
76-77	0.012854042758157165
78-79	0.013043881078814297
80-81	0.010365273152753077
82-83	0.0050121913723583595
84-85	0.0035488524146606187
86-87	0.014140453679867859
88-89	0.001390211065932886
90-91	2.8267307968745335E-4
92-93	0.0012947833180117308
94-95	0.0032116012707235696
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	197.0
20-21	636.0
22-23	1363.0
24-25	3682.0
26-27	4843.0
28-29	10733.0
30-31	23207.0
32-33	28933.0
34-35	40277.0
36-37	60327.0
38-39	71650.0
40-41	72207.0
42-43	78352.0
44-45	90237.0
46-47	88468.0
48-49	84075.0
50-51	78353.0
52-53	75029.0
54-55	80240.0
56-57	83305.0
58-59	82314.0
60-61	81164.0
62-63	78830.0
64-65	81754.0
66-67	85784.0
68-69	86329.0
70-71	85307.0
72-73	80950.0
74-75	82189.0
76-77	83734.0
78-79	88225.0
80-81	89724.0
82-83	87255.0
84-85	87135.0
86-87	93442.0
88-89	98778.0
90-91	104236.0
92-93	112069.0
94-95	138837.0
96-97	308940.0
98-99	533713.0
100-101	4842090.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.08359415611449
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.18954379922938	74.993774037834
2	7.268417764917241	12.22309379004788
3	1.9378762909902778	4.888308107291487
4	0.7537502535239741	2.535121216495129
5	0.3264213669649429	1.3723340871882186
6	0.18091386738984855	0.9127132921692683
7	0.10688826390460489	0.6291284581544521
8	0.07185251732920202	0.48332943249630445
9	0.03897537369367773	0.29494705533678883
>10	0.12351160771869468	1.507374403175483
>50	0.0015329011059314064	0.08580746244599151
>100	2.465827141510823E-4	0.037630800406919976
>500	6.941051810462603E-5	0.03643785695816053
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	1.1920495539767787E-5	5.960247769883893E-5	0.0	0.0	0.0
3	1.1920495539767787E-5	7.152297323860671E-5	0.0	0.0	0.0
4	1.1920495539767787E-5	1.0728445985791008E-4	0.0	1.1920495539767787E-5	0.0
5	1.1920495539767787E-5	1.1920495539767786E-4	0.0	1.1920495539767787E-5	0.0
6	5.960247769883893E-5	1.1920495539767786E-4	0.0	1.1920495539767787E-5	0.0
7	5.960247769883893E-5	1.1920495539767786E-4	0.0	1.1920495539767787E-5	0.0
8	7.152297323860671E-5	1.1920495539767786E-4	0.0	2.3840991079535573E-5	0.0
9	7.152297323860671E-5	1.788074330965168E-4	0.0	9.53639643181423E-5	0.0
10-11	7.152297323860671E-5	1.788074330965168E-4	0.0	9.53639643181423E-5	0.0
12-13	7.152297323860671E-5	1.788074330965168E-4	0.0	1.1920495539767786E-4	0.0
14-15	8.344346877837451E-5	2.4437015856523965E-4	0.0	1.1920495539767786E-4	5.960247769883893E-6
16-17	1.6688693755674902E-4	2.5033040633512353E-4	0.0	1.2516520316756177E-4	1.1920495539767787E-5
18-19	1.6688693755674902E-4	2.80131645184543E-4	0.0	1.3112545093744564E-4	1.7880743309651682E-5
20-21	1.728471853266329E-4	3.099328840339624E-4	0.0	1.6092668978686512E-4	2.3840991079535573E-5
22-23	1.907279286362846E-4	3.576148661930336E-4	0.0	2.3244966302547184E-4	3.5761486619303364E-5
24-25	2.0264842417605237E-4	3.814558572725692E-4	0.0	2.622509018748913E-4	4.768198215907115E-5
26-27	2.1456891971582015E-4	4.0529684835210474E-4	0.0	3.1589313180384635E-4	4.768198215907115E-5
28-29	2.3840991079535572E-4	4.112570961219886E-4	0.0	7.450309712354866E-4	5.364222992895504E-5
30-31	2.3840991079535572E-4	4.529788305111759E-4	0.0	0.0022470134092462276	5.960247769883893E-5
32-33	2.3840991079535572E-4	4.7681982159071144E-4	0.0	0.00511389258656038	8.344346877837451E-5
34-35	2.3840991079535572E-4	5.364222992895503E-4	0.0	0.00866620025741118	9.53639643181423E-5
36-37	2.5033040633512353E-4	5.960247769883893E-4	0.0	0.014113866719085058	9.53639643181423E-5
38-39	2.741713974146591E-4	5.960247769883893E-4	0.0	0.02227344591605611	9.53639643181423E-5
40-41	2.741713974146591E-4	6.317862636076927E-4	0.0	0.030671435023822517	1.0728445985791008E-4
42-43	2.9801238849419466E-4	6.496670069173443E-4	0.0	0.03798465903747005	1.0728445985791008E-4
44-45	2.9801238849419466E-4	7.211899801559511E-4	0.0	0.046835626975747634	1.0728445985791008E-4
46-47	3.099328840339624E-4	7.629117145451384E-4	0.0	0.05618129547892557	1.0728445985791008E-4
48-49	3.3377387511349804E-4	7.629117145451384E-4	0.0	0.0660514657858533	1.0728445985791008E-4
50-51	3.456943706532658E-4	8.403949355536289E-4	0.0	0.07601700005709917	1.0728445985791008E-4
52-53	3.6953536173280136E-4	8.463551833235129E-4	0.0	0.08546995302013502	1.1324470762779397E-4
54-55	3.754956095026853E-4	8.642359266331646E-4	0.0	0.09449972839150914	1.1920495539767786E-4
56-57	3.8741610504245305E-4	9.059576610223518E-4	0.0	0.10220036851019912	1.2516520316756177E-4
58-59	3.993366005822208E-4	9.178781565621195E-4	0.0	0.10974604218687212	1.3112545093744564E-4
60-61	4.4105833497140806E-4	9.238384043320034E-4	0.0	0.11719635189922699	1.3112545093744564E-4
62-63	4.887403171304792E-4	9.357588998717712E-4	0.0	0.12413408030337184	1.4304594647721343E-4
64-65	5.006608126702471E-4	0.0010490036074995652	0.0	0.1305175056649175	1.4304594647721343E-4
66-67	5.245018037497826E-4	0.0010490036074995652	0.0	0.13750291605122142	1.4304594647721343E-4
68-69	5.245018037497826E-4	0.0010609241030393331	0.0	0.14436912148212766	1.4304594647721343E-4
70-71	5.423825470594343E-4	0.0010907253418887524	0.0	0.15068698411820458	1.549664420169812E-4
72-73	5.841042814486216E-4	0.0011086060851984041	0.0	0.1556757115015974	1.6688693755674902E-4
74-75	5.960247769883893E-4	0.0011443675718177074	0.0	0.15964523651634008	1.6688693755674902E-4
76-77	6.13905520298041E-4	0.0011920495539767787	0.0	0.1633346298858982	1.6688693755674902E-4
78-79	6.198657680679248E-4	0.001227811040596082	0.0	0.16590349667471815	1.6688693755674902E-4
80-81	6.198657680679248E-4	0.001227811040596082	0.0	0.16656508417717528	1.728471853266329E-4
82-83	6.198657680679248E-4	0.001227811040596082	0.0	0.16702402325545634	1.788074330965168E-4
84-85	6.198657680679248E-4	0.001233771288365966	0.0	0.16720879093632274	1.788074330965168E-4
86-87	6.258260158378088E-4	0.0012516520316756177	0.0	0.16728627415733122	1.788074330965168E-4
88	6.317862636076927E-4	0.0012993340138346887	0.0	0.1672922344051011	1.788074330965168E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9180	0.0	43.64427	1
GTATCAA	15935	0.0	33.957413	1
ATCAACG	18310	0.0	28.712614	3
TCAACGC	18390	0.0	28.632254	4
CAACGCA	18600	0.0	28.264103	5
TATCAAC	19370	0.0	27.400784	2
AACGCAG	19385	0.0	27.247768	6
CGCAGAG	22830	0.0	23.081697	8
ACGCAGA	22830	0.0	23.063553	7
GTACATG	23420	0.0	21.60092	1
TACATGG	23440	0.0	21.03453	2
ACATGGG	24020	0.0	20.007114	3
GCAGAGT	27935	0.0	18.655998	9
CATGGGG	18685	0.0	18.557695	4
ACCGAAC	1300	0.0	18.164274	8
GAGTACT	15025	0.0	17.578037	12-13
CAGAGTA	26700	0.0	17.067425	10-11
AGAGTAC	24830	0.0	16.384052	10-11
GTGGTAT	5345	0.0	16.123493	1
ACGGGCG	875	0.0	16.097462	5
>>END_MODULE