FastQCFastQC Report
Fri 27 May 2016
SRR2140303_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140303_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7963802
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77650.045.44751
GTATCAA136050.033.790131
TCAACGC156150.028.5691344
ATCAACG157700.028.3677233
CAACGCA160800.027.7429735
TATCAAC167550.026.9225522
AACGCAG168250.026.538956
ACGCAGA201350.022.2170037
CGCAGAG204150.021.9929128
GTACATG212750.021.6661431
TACATGG213900.020.9350512
ACATGGG214600.020.310193
GCAGAGT244100.018.3766699
CATGGGG159150.018.0676064
GAGTACT135500.017.12912-13
CAGAGTA240450.016.4178410-11
AGAGTAC217750.016.28058610-11
GTGGTAT48250.015.3261661
GAGTACA155300.015.1579841
CGTCGAA3900.003870935914.93213394
Produced by FastQC (version 0.11.5)