##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140303_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7963802
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.024894390895206	33.0	31.0	34.0	30.0	34.0
2	32.13285425730072	33.0	31.0	34.0	30.0	34.0
3	31.869279648087684	31.0	31.0	34.0	30.0	34.0
4	35.92180531359268	37.0	35.0	37.0	35.0	37.0
5	35.862491684248305	37.0	35.0	37.0	35.0	37.0
6	35.9060108475826	37.0	35.0	37.0	35.0	37.0
7	35.890790479220854	37.0	35.0	37.0	35.0	37.0
8	35.88938524589135	37.0	35.0	37.0	35.0	37.0
9	37.67110671510919	39.0	37.0	39.0	35.0	39.0
10-11	37.58322770957892	39.0	37.0	39.0	35.0	39.0
12-13	37.53900888043174	39.0	37.0	39.0	35.0	39.0
14-15	38.804691653559445	40.0	38.0	41.0	35.0	41.0
16-17	38.7467672476036	40.0	38.0	41.0	34.0	41.0
18-19	38.74480449162347	40.0	38.0	41.0	34.0	41.0
20-21	38.806919652009796	40.0	38.0	41.0	34.5	41.0
22-23	38.76752219581306	40.0	38.0	41.0	34.0	41.0
24-25	38.677906441156395	40.0	38.0	41.0	34.0	41.0
26-27	38.53643183736564	40.0	38.0	41.0	34.0	41.0
28-29	38.41413467485401	40.0	38.0	41.0	34.0	41.0
30-31	38.29669455515979	40.0	38.0	41.0	34.0	41.0
32-33	38.11298831690212	40.0	37.5	41.0	33.5	41.0
34-35	38.20121823148486	40.0	38.0	41.0	34.0	41.0
36-37	38.1629638209058	40.0	38.0	41.0	33.0	41.0
38-39	38.13010676455843	40.0	38.0	41.0	33.0	41.0
40-41	38.07155228149864	40.0	37.5	41.0	33.0	41.0
42-43	38.097762108753855	40.0	38.0	41.0	33.0	41.0
44-45	38.12546778205281	40.0	38.0	41.0	33.0	41.0
46-47	38.07050359710102	40.0	37.0	41.0	33.0	41.0
48-49	37.982785816623625	40.0	37.0	41.0	33.0	41.0
50-51	37.89121602279393	40.0	37.0	41.0	33.0	41.0
52-53	37.630436297363794	40.0	37.0	41.0	32.0	41.0
54-55	37.57823258020815	39.5	36.0	41.0	32.0	41.0
56-57	37.4165499737531	39.0	36.0	41.0	32.0	41.0
58-59	37.24919113529507	39.0	36.0	41.0	31.0	41.0
60-61	37.102023391673775	39.0	35.0	41.0	31.0	41.0
62-63	36.863373338387795	38.5	35.0	40.0	31.0	41.0
64-65	36.50817814884796	38.0	35.0	40.0	31.0	41.0
66-67	36.26394583072455	37.5	35.0	40.0	31.0	41.0
68-69	35.973858561501174	37.0	34.5	40.0	30.0	41.0
70-71	35.69745258840593	36.5	34.0	39.0	30.0	41.0
72-73	35.402500600272965	36.0	34.0	39.0	30.0	40.5
74-75	35.07825659414182	36.0	34.0	38.5	30.0	40.0
76-77	33.832440184334345	34.5	32.5	37.0	28.5	39.0
78-79	34.4358494188744	35.0	34.0	37.0	29.0	39.0
80-81	34.35868152532639	35.0	34.0	37.0	30.0	39.0
82-83	34.08033180251681	35.0	34.0	36.0	29.0	38.5
84-85	33.84045564319168	35.0	33.5	36.0	29.0	37.0
86-87	33.6356904117724	35.0	33.0	35.5	29.0	37.0
88-89	33.461987441374895	35.0	33.0	35.0	29.0	36.5
90-91	33.325604453437805	35.0	33.0	35.0	29.0	36.0
92-93	33.29497669589533	35.0	33.0	35.0	29.0	36.0
94-95	33.285903718324	35.0	33.0	35.0	29.0	36.0
96-97	33.26606755113656	35.0	33.0	35.0	29.0	35.0
98-99	33.36506758573597	35.0	33.0	35.0	29.5	35.0
100	32.24908419483346	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	8.0
20	36.0
21	215.0
22	1092.0
23	3702.0
24	9833.0
25	21628.0
26	39595.0
27	63525.0
28	92536.0
29	124132.0
30	159625.0
31	200038.0
32	249431.0
33	315321.0
34	422554.0
35	584121.0
36	899897.0
37	1544881.0
38	2022843.0
39	1161817.0
40	46971.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.70641108355029	20.351636065286407	13.631416250680264	24.310536600483033
2	15.009738608465243	21.283587525037202	40.57596554127449	23.13070832522306
3	18.28412382888579	25.82689673098874	30.48033733614447	25.408642103981006
4	12.679035673529743	17.691908963788034	38.69266287750936	30.936392485172863
5	13.131529387596528	37.906128253816455	35.88051285052039	13.081829508066626
6	30.52803171148655	37.829368434825476	18.503963307977774	13.138636545710202
7	26.919504025840922	32.401295260731	23.081588417190684	17.5976122962374
8	24.67378018690068	36.72723154091476	21.143129876910553	17.455858395274014
9	25.558520917521555	16.53884664636313	21.308113888316157	36.594518547799154
10-11	24.360763866304058	26.71436331541141	28.94577238359266	19.979100434691873
12-13	24.910469898674027	24.140485913637733	29.129924375317216	21.819119812371028
14-15	21.95733162761303	25.708930583436956	26.562004201265456	25.771733587684558
16-17	21.746024152119855	28.876049078746966	27.31921163498759	22.058715134145586
18-19	21.762940215192458	28.25624065913395	28.752199678991904	21.22861944668169
20-21	22.481947672359798	27.39511667961739	28.678908914102763	21.444026733920044
22-23	22.256458964322746	27.335431618423566	28.462041673589578	21.94606774366411
24-25	22.055622176871996	27.395286845066646	28.579077227706357	21.970013750355
26-27	22.046227002607164	27.60667276864408	28.418389067910383	21.928711160838375
28-29	21.96871092504531	27.727680587083086	28.422746753456202	21.8808617344154
30-31	22.194606159778683	27.57115835092472	28.3486221660384	21.8856133232582
32-33	21.969235142241278	27.669479119785866	28.35407537248028	22.007210365492575
34-35	22.087472002514115	27.70606853472563	28.38190162361005	21.824557839150202
36-37	22.109139727929012	27.62974459314758	28.29155267773107	21.969563001192334
38-39	21.973168207223203	27.788041296871913	28.33770015978515	21.90109033611973
40-41	22.230200859915357	27.651668954190896	28.175666291031337	21.942463894862417
42-43	22.082953473750393	27.726494550979773	28.082337839253903	22.108214136015928
44-45	22.19074498666533	27.794728501175726	27.94230383135186	22.072222680807087
46-47	22.179888535955588	27.760579927067003	27.788835133882657	22.270696403094757
48-49	22.16091055519155	27.853859828717773	27.752914185221833	22.23231543086884
50-51	22.129616658062787	27.94504245144519	27.70292295245696	22.222417938035065
52-53	22.280299408152956	27.868737663792732	27.572644447173417	22.278318480880902
54-55	22.234118314407276	27.77658886860393	27.656384200748164	22.33290861624063
56-57	22.250826022970443	27.91022880506991	27.619532736342983	22.21941243561666
58-59	22.223118872760246	27.962964383057802	27.620647734137414	22.193269010044535
60-61	22.30947410451656	27.899210780762772	27.621382461129162	22.169932653591502
62-63	22.252602824356543	27.980250855807252	27.55412836430112	22.21301795553508
64-65	22.203970297225204	27.957038256196455	27.55591242856869	22.283079018009648
66-67	22.27858519206343	27.94019698289134	27.466631264193065	22.314586560852163
68-69	22.2414090475565	27.91935898387751	27.53153585346027	22.30769611510572
70-71	22.22719773799543	27.737693757398496	27.596665660333453	22.438442844272625
72-73	22.294795541125296	27.78849261949823	27.527842957777743	22.388868881598732
74-75	22.16683298474994	27.91600948666001	27.518785079443692	22.398372449146358
76-77	22.254968712329763	27.890734636598026	27.45948223249878	22.394814418573432
78-79	22.209656766906814	27.82051956283704	27.483406269962607	22.486417400293547
80-81	22.19693034316369	27.88291140806119	27.498437466502416	22.421720782272708
82-83	22.22566417802944	27.89383759036249	27.57414003482408	22.306358196783986
84-85	22.331997647309812	27.835836468174485	27.52639881493118	22.30576706958452
86-87	22.185398689065376	27.860823583385546	27.589312955794586	22.364464771754488
88-89	22.265883648992585	27.877037730158904	27.5106633123769	22.346415308471613
90-91	22.20867613428701	27.939419219764922	27.53514966458519	22.316754981362877
92-93	22.270017180611347	27.88021001245583	27.593766734545728	22.2560060723871
94-95	22.104007059762715	27.9774577947056	27.62453447166544	22.29400067386624
96-97	22.12076161418182	28.062776405074814	27.749673730301627	22.066788250441743
98-99	23.221259072980118	30.05225585093309	27.948499081206617	18.777985994880172
100	29.715185852094983	41.42993864616345	28.85487550174156	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1484.0
1	1322.5
2	1690.0
3	2353.5
4	3059.5
5	4971.0
6	6766.0
7	7964.5
8	8662.0
9	8490.0
10	7779.0
11	7030.5
12	7530.5
13	9811.5
14	15162.0
15	22590.0
16	29002.5
17	33298.0
18	35295.0
19	35289.0
20	34311.5
21	33384.0
22	33834.0
23	36925.0
24	43349.5
25	53815.5
26	68104.0
27	84517.5
28	102157.5
29	122421.5
30	143333.5
31	163717.0
32	183995.0
33	203406.0
34	222765.5
35	241475.0
36	260936.5
37	278543.5
38	291680.5
39	302242.0
40	311718.5
41	320228.5
42	327623.5
43	335003.5
44	341839.0
45	346459.5
46	347828.5
47	347957.0
48	345340.0
49	340004.0
50	334678.5
51	325335.5
52	311250.5
53	294840.5
54	276848.0
55	257588.0
56	236555.0
57	213785.5
58	190551.5
59	165373.5
60	139215.0
61	114736.5
62	92601.0
63	72800.0
64	55479.0
65	42314.0
66	31872.0
67	24473.5
68	18973.5
69	14073.0
70	10406.5
71	7607.0
72	5415.5
73	3734.0
74	2570.5
75	1797.5
76	1231.5
77	792.5
78	529.0
79	354.5
80	237.5
81	163.0
82	116.0
83	83.5
84	60.0
85	41.0
86	29.0
87	21.5
88	16.0
89	12.5
90	10.0
91	6.0
92	3.5
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.008187044328826859
3	0.002913181417619373
4	3.2647722783665393E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	1.3812498100781512E-4
16-17	3.6414767720242163E-4
18-19	0.0017579543037358287
20-21	3.767178806253869E-5
22-23	0.0017518891675795012
24-25	2.8889855791888535E-4
26-27	0.002557085359970079
28-29	0.005482414432682332
30-31	0.00283402298757917
32-33	0.002052843345945161
34-35	0.0023017617227678285
36-37	0.002856882494573677
38-39	0.0
40-41	6.616985217200919E-4
42-43	0.006424453631653264
44-45	0.004057594274198321
46-47	6.162785697031828E-4
48-49	0.016387762936166613
50-51	0.01106776066855031
52-53	0.01569950034453028
54-55	0.013446049633211924
56-57	0.011690209587543774
58-59	0.017956860398451697
60-61	0.009412106623070291
62-63	0.020598964784646553
64-65	0.015333917199815934
66-67	0.031052717172874056
68-69	0.02219990558188305
70-71	0.022261419255726552
72-73	0.019737671807994177
74-75	0.0097573251159721
76-77	0.012122768041657423
78-79	0.012906081356913537
80-81	0.010083302257579646
82-83	0.005094355579494658
84-85	0.0029955084345201373
86-87	0.014322890312756633
88-89	0.0014439830408129993
90-91	1.7806846465363233E-4
92-93	0.0011789143015454205
94-95	0.0027776072635540983
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	184.0
20-21	549.0
22-23	1185.0
24-25	2711.0
26-27	4433.0
28-29	10322.0
30-31	22156.0
32-33	28492.0
34-35	39191.0
36-37	59948.0
38-39	69933.0
40-41	71344.0
42-43	78085.0
44-45	88573.0
46-47	88228.0
48-49	82895.0
50-51	77189.0
52-53	75149.0
54-55	80799.0
56-57	83890.0
58-59	82707.0
60-61	80489.0
62-63	78452.0
64-65	82412.0
66-67	85477.0
68-69	85582.0
70-71	84343.0
72-73	80459.0
74-75	82267.0
76-77	82445.0
78-79	87207.0
80-81	88081.0
82-83	85451.0
84-85	85638.0
86-87	90317.0
88-89	96142.0
90-91	100999.0
92-93	109074.0
94-95	132566.0
96-97	291671.0
98-99	1630776.0
100-101	3375991.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.71210770214094
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.13501124282408	77.25661791378623
2	6.867762683208007	11.773008295517384
3	1.6719134754807308	4.299096836371955
4	0.6190079046903244	2.122258887811747
5	0.285496061087637	1.2235234568240276
6	0.1432611522360607	0.7367529185994026
7	0.08096923238937348	0.48580304969823607
8	0.05586726139431176	0.3830800580522328
9	0.03203638595345478	0.24713155469068843
>10	0.10745093959690988	1.3876659769686284
>50	0.0011060024890427664	0.06243739356885552
>100	1.176586500352424E-4	0.02262365811064667
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	1.255681645525592E-5	0.0	1.255681645525592E-5	0.0
5	0.0	1.255681645525592E-5	0.0	1.255681645525592E-5	0.0
6	0.0	1.255681645525592E-5	0.0	1.255681645525592E-5	0.0
7	0.0	1.255681645525592E-5	0.0	1.255681645525592E-5	0.0
8	0.0	2.511363291051184E-5	0.0	1.255681645525592E-5	0.0
9	0.0	1.1301134809730327E-4	0.0	3.767044936576776E-5	0.0
10-11	0.0	1.1301134809730327E-4	0.0	5.650567404865164E-5	0.0
12-13	0.0	1.1301134809730327E-4	0.0	8.161930695916347E-5	0.0
14-15	0.0	1.5068179746307103E-4	0.0	8.789771518679144E-5	0.0
16-17	0.0	1.5068179746307103E-4	0.0	1.3812498100781512E-4	0.0
18-19	0.0	1.6323861391832694E-4	0.0	1.3812498100781512E-4	1.883522468288388E-5
20-21	0.0	1.8207383860121082E-4	0.0	1.5068179746307103E-4	6.27840822762796E-5
22-23	0.0	2.2602269619460653E-4	0.0	1.883522468288388E-4	6.27840822762796E-5
24-25	0.0	2.5741473733274637E-4	0.0	2.0090906328409472E-4	6.27840822762796E-5
26-27	0.0	2.636931455603743E-4	0.0	2.7624996201563025E-4	6.27840822762796E-5
28-29	0.0	2.636931455603743E-4	0.0	6.780680885838196E-4	6.906249050390756E-5
30-31	0.0	2.8880677847088613E-4	0.0	0.002166050838531646	7.534089873153552E-5
32-33	0.0	2.8880677847088613E-4	0.0	0.00529269813589037	7.534089873153552E-5
34-35	0.0	3.5159086074716575E-4	0.0	0.008695595395264724	7.534089873153552E-5
36-37	0.0	4.143749430234453E-4	0.0	0.01396945830647221	7.534089873153552E-5
38-39	0.0	4.457669841615851E-4	0.0	0.02252692872072912	9.41761234144194E-5
40-41	0.0	5.022726582102368E-4	0.0	0.031366927505229285	1.0045453164204736E-4
42-43	0.0	5.336646993483766E-4	0.0	0.03939073322013782	1.0045453164204736E-4
44-45	0.0	6.21562414535168E-4	0.0	0.048927635317904686	1.0045453164204736E-4
46-47	0.0	6.780680885838196E-4	0.0	0.05946908273209203	1.0045453164204736E-4
48-49	0.0	6.780680885838196E-4	0.0	0.06944547340579285	1.0045453164204736E-4
50-51	0.0	7.91079436681123E-4	0.0	0.07871868235799936	1.0045453164204736E-4
52-53	0.0	8.036362531363789E-4	0.0	0.08868251621524492	1.0045453164204736E-4
54-55	0.0	8.161930695916348E-4	0.0	0.09828220239528808	1.0045453164204736E-4
56-57	0.0	8.413067025021466E-4	0.0	0.10656342284752936	1.0673293986967531E-4
58-59	0.0	8.413067025021466E-4	0.0	0.11461862060357603	1.1301134809730327E-4
60-61	0.0	8.789771518679143E-4	0.0	0.12235989794824131	1.1301134809730327E-4
62-63	0.0	8.978123765507981E-4	0.0	0.12990654463785012	1.1301134809730327E-4
64-65	0.0	0.0010045453164204735	0.0	0.13750341859327994	1.1301134809730327E-4
66-67	0.0	0.0010171021328757295	0.0	0.14506890050757165	1.1301134809730327E-4
68-69	0.0	0.0010359373575586132	0.0	0.15243347335857924	1.1301134809730327E-4
70-71	0.0	0.0010422157657862413	0.0	0.1591136997127754	1.1301134809730327E-4
72-73	0.0	0.0010547725822414972	0.0	0.1640359717632357	1.1301134809730327E-4
74-75	0.0	0.0010547725822414972	0.0	0.16807298825360048	1.1301134809730327E-4
76-77	0.0	0.0010673293986967532	0.0	0.17221673768383494	1.1301134809730327E-4
78-79	0.0	0.001092443031607265	0.0	0.17478460664893475	1.1301134809730327E-4
80-81	0.0	0.001092443031607265	0.0	0.17565730539257507	1.3184657278018716E-4
82-83	0.0	0.001104999848062521	0.0	0.17626631099065498	1.5068179746307103E-4
84-85	0.0	0.0011175566645177768	0.0	0.1764734984621667	1.5068179746307103E-4
86-87	0.0	0.0011301134809730327	0.0	0.17657395299380874	1.56960205690699E-4
88	0.0	0.0011552271138835445	0.0	0.17657395299380874	1.6323861391832694E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7765	0.0	45.4475	1
GTATCAA	13605	0.0	33.79013	1
TCAACGC	15615	0.0	28.569134	4
ATCAACG	15770	0.0	28.367723	3
CAACGCA	16080	0.0	27.742973	5
TATCAAC	16755	0.0	26.922552	2
AACGCAG	16825	0.0	26.53895	6
ACGCAGA	20135	0.0	22.217003	7
CGCAGAG	20415	0.0	21.992912	8
GTACATG	21275	0.0	21.666143	1
TACATGG	21390	0.0	20.935051	2
ACATGGG	21460	0.0	20.31019	3
GCAGAGT	24410	0.0	18.376669	9
CATGGGG	15915	0.0	18.067606	4
GAGTACT	13550	0.0	17.129	12-13
CAGAGTA	24045	0.0	16.41784	10-11
AGAGTAC	21775	0.0	16.280586	10-11
GTGGTAT	4825	0.0	15.326166	1
GAGTACA	15530	0.0	15.157984	1
CGTCGAA	390	0.0038709359	14.932133	94
>>END_MODULE