FastQCFastQC Report
Fri 27 May 2016
SRR2140302_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140302_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5198903
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52100.042.9578441
GTATCAA88950.033.2259061
TCAACGC103350.027.8396644
ATCAACG103750.027.7731723
CAACGCA105150.027.2848765
AACGCAG110650.025.9286466
TATCAAC112350.025.6854462
TACATGG148750.022.2935432
ACGCAGA133250.021.561757
GTACATG158500.021.5169931
ACATGGG153300.020.8817623
CGCAGAG139100.020.6254838
CATGGGG110950.018.4705624
GTGGTAT31450.018.1168081
GCAGAGT160800.017.6891379
TGGTATC31300.017.28692
GAGTACT83950.016.86877412-13
ACCGAAC6950.016.5122558
GGACCGA8100.016.1919146
AGAGTAC146150.016.04111710-11
Produced by FastQC (version 0.11.5)