##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140302_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5198903
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.99368039757618	31.0	31.0	34.0	30.0	34.0
2	32.1025631753468	33.0	31.0	34.0	30.0	34.0
3	31.84140346530797	31.0	31.0	34.0	30.0	34.0
4	35.89933914904741	37.0	35.0	37.0	35.0	37.0
5	35.83911779081087	37.0	35.0	37.0	35.0	37.0
6	35.88138997784725	37.0	35.0	37.0	35.0	37.0
7	35.8649682442623	37.0	35.0	37.0	35.0	37.0
8	35.86247387189182	37.0	35.0	37.0	35.0	37.0
9	37.63898980227175	39.0	37.0	39.0	35.0	39.0
10-11	37.549316173046506	39.0	37.0	39.0	35.0	39.0
12-13	37.50188789827392	39.0	37.0	39.0	35.0	39.0
14-15	38.75741815148311	40.0	38.0	41.0	34.5	41.0
16-17	38.696869416490365	40.0	38.0	41.0	34.0	41.0
18-19	38.698240186439335	40.0	38.0	41.0	34.0	41.0
20-21	38.75792453969443	40.0	38.0	41.0	34.0	41.0
22-23	38.716691250333525	40.0	38.0	41.0	34.0	41.0
24-25	38.62673500686948	40.0	38.0	41.0	34.0	41.0
26-27	38.48348565758248	40.0	38.0	41.0	34.0	41.0
28-29	38.35731343839261	40.0	38.0	41.0	34.0	41.0
30-31	38.241252415176746	40.0	38.0	41.0	34.0	41.0
32-33	38.05747528011649	40.0	37.5	41.0	33.0	41.0
34-35	38.143524667108125	40.0	38.0	41.0	33.0	41.0
36-37	38.10266515237283	40.0	38.0	41.0	33.0	41.0
38-39	38.06623085358776	40.0	37.0	41.0	33.0	41.0
40-41	38.00330542601351	40.0	37.0	41.0	33.0	41.0
42-43	38.03767586187454	40.0	37.0	41.0	33.0	41.0
44-45	38.065029168330945	40.0	37.0	41.0	33.0	41.0
46-47	38.011468593092914	40.0	37.0	41.0	33.0	41.0
48-49	37.919088302513	40.0	37.0	41.0	33.0	41.0
50-51	37.8274818451046	40.0	37.0	41.0	33.0	41.0
52-53	37.564783173424324	40.0	36.0	41.0	32.0	41.0
54-55	37.5085370534652	39.0	36.0	41.0	32.0	41.0
56-57	37.34407785002104	39.0	36.0	41.0	31.5	41.0
58-59	37.173762950025	39.0	35.5	41.0	31.0	41.0
60-61	37.02710541363763	39.0	35.0	40.5	31.0	41.0
62-63	36.78147898757395	38.5	35.0	40.0	31.0	41.0
64-65	36.420676063421894	38.0	35.0	40.0	31.0	41.0
66-67	36.173768068135416	37.5	35.0	40.0	30.0	41.0
68-69	35.87996575987029	37.0	34.0	39.5	30.0	41.0
70-71	35.60560114703583	36.5	34.0	39.0	30.0	41.0
72-73	35.310056889640464	36.0	34.0	39.0	30.0	40.5
74-75	34.988121639892924	35.5	34.0	38.5	29.5	40.0
76-77	33.75010739283826	34.5	32.5	36.5	28.5	39.0
78-79	34.36168232412504	35.0	33.5	37.0	29.0	39.0
80-81	34.292886674624626	35.0	34.0	37.0	29.0	39.0
82-83	34.020126922326014	35.0	34.0	36.0	29.0	38.0
84-85	33.7796259498013	35.0	33.0	36.0	29.0	37.0
86-87	33.58134189637734	35.0	33.0	35.5	29.0	37.0
88-89	33.40968338658373	35.0	33.0	35.0	29.0	36.5
90-91	33.27786070544727	35.0	33.0	35.0	29.0	36.0
92-93	33.24945412439983	35.0	33.0	35.0	29.0	36.0
94-95	33.24507340287397	35.0	33.0	35.0	29.0	36.0
96-97	33.234719035770745	35.0	33.0	35.0	29.0	35.0
98-99	33.3374707261153	35.0	33.0	35.0	29.5	35.0
100	32.22977636076933	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	5.0
20	30.0
21	168.0
22	751.0
23	2658.0
24	6879.0
25	14850.0
26	26752.0
27	42690.0
28	62207.0
29	83831.0
30	106535.0
31	133697.0
32	166085.0
33	211160.0
34	282039.0
35	387676.0
36	598026.0
37	1014608.0
38	1298801.0
39	730394.0
40	29061.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.147333774067334	20.225285988217127	13.275935327125744	24.35144491058979
2	15.249834759264061	21.41019791184955	40.13212718496729	23.207840143919103
3	18.507000120028437	25.789994860320753	30.327973858421842	25.375031161228968
4	12.695772935709229	17.417693810974672	38.60119871972425	31.28533453359185
5	13.113574152085548	38.02090556411612	35.76831496952338	13.097205314274953
6	30.7292711558573	37.725977961119874	18.378107843135368	13.166643039887454
7	27.13064659986924	32.32631961011775	22.970999843620856	17.572033946392153
8	24.98205871507893	36.50220056038745	21.028897827099293	17.486842897434325
9	25.72811995145899	16.282338793395454	21.095354154520674	36.89418710062488
10-11	24.565326569855216	26.656988984022206	28.77724396858337	20.000440477539204
12-13	25.010795546675908	24.10157488993351	28.975208808473635	21.91242075491695
14-15	22.045925143708423	25.547198010346428	26.488417250204133	25.918459595741016
16-17	21.87118848414169	28.711427050772254	27.164979477322344	22.25240498776372
18-19	21.88900256846598	28.120182202779358	28.617727512554286	21.373087716200377
20-21	22.71709097819915	27.31837945515568	28.43767937190403	21.526850194741144
22-23	22.369604424672357	27.22484549717446	28.335130455693157	22.070419622460022
24-25	22.224628569685777	27.269324897027623	28.48642465652864	22.01962187675796
26-27	22.1457555035439	27.458648101610876	28.34161956291984	22.053976831925386
28-29	22.06391100930422	27.639421229112703	28.330624940397698	21.96604282118538
30-31	22.373710366872075	27.40737788925775	28.276627717075097	21.942284026795075
32-33	22.191355268083996	27.523676697744033	28.183284875043025	22.10168315912895
34-35	22.237493117411425	27.593817890883432	28.24354008104843	21.925148910656713
36-37	22.320469926843085	27.44508834966196	28.1721146336693	22.062327089825658
38-39	22.09555996268386	27.67105659193984	28.255903923552346	21.977479521823952
40-41	22.313248183605666	27.520533520673073	28.12901990743143	22.037198388289838
42-43	22.2501952475693	27.59637121994133	27.965402421722107	22.18803111076726
44-45	22.29730271128559	27.713311584085957	27.82084408244158	22.16854162218687
46-47	22.262345667289015	27.612919306739965	27.656117939659268	22.468617086311752
48-49	22.29533600800323	27.732271722410662	27.695977479905686	22.276414789680423
50-51	22.21908487687922	27.783479176106724	27.647668435162664	22.34976751185139
52-53	22.425401788090184	27.75169590038211	27.503930163290196	22.318972148237506
54-55	22.434760701448575	27.68035436475588	27.49993100291055	22.384953930884997
56-57	22.433617840592834	27.841965398564643	27.392138262290562	22.33227849855196
58-59	22.32708976682846	27.87688224087974	27.53470263856464	22.26132535372716
60-61	22.406315134345625	27.796434572233093	27.49219863590568	22.305051657515605
62-63	22.360312935639595	27.830802006363058	27.478897091035105	22.329987966962243
64-65	22.34928361668661	27.793338126945216	27.4868154755317	22.370562780836476
66-67	22.458205355152746	27.832629973576612	27.276254371142734	22.432910300127908
68-69	22.406073339751025	27.839968017787058	27.387617015812072	22.366341626649845
70-71	22.336856391615942	27.596471460946898	27.48176732988742	22.584904817549745
72-73	22.415106561921867	27.63861237419878	27.447576928610946	22.49870413526841
74-75	22.243125676765096	27.826385120188355	27.390767048625353	22.53972215442119
76-77	22.324108161766095	27.863180309467104	27.28527113704931	22.527440391717487
78-79	22.321450069524843	27.831193580037844	27.307236603174665	22.540119747262647
80-81	22.257520238472296	27.78913594529884	27.40338541852315	22.549958397705712
82-83	22.39725768066122	27.71714992416547	27.471949234993232	22.41364316018008
84-85	22.461516228406133	27.758948379296953	27.366317718461787	22.413217673835124
86-87	22.28917403714516	27.839731366386623	27.411427397096432	22.459667199371783
88-89	22.395514495117336	27.75499576112314	27.387287919058778	22.462201824700742
90-91	22.339056217024122	27.801439127805338	27.456635167164162	22.40286948800637
92-93	22.428789091717967	27.791860072808248	27.459772716957392	22.31957811851639
94-95	22.151413789444984	27.959282909925577	27.468760359196825	22.420542941432608
96-97	22.16995301388769	28.08564410473894	27.595456432295883	22.148946449077485
98-99	23.322326611689835	30.048285198189934	27.764094403118545	18.865293787001686
100	29.806056560122467	41.5295864458282	28.664356994049335	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	913.0
1	829.0
2	1160.0
3	1708.5
4	2329.0
5	3757.5
6	5010.5
7	5770.5
8	6200.5
9	6076.5
10	5587.0
11	5018.5
12	5182.0
13	6564.5
14	10014.5
15	14873.0
16	19024.0
17	21696.5
18	22879.0
19	22744.0
20	21671.5
21	20435.5
22	20371.5
23	22284.0
24	26598.0
25	33590.5
26	43583.5
27	55144.5
28	66096.5
29	78232.5
30	89939.5
31	100515.0
32	113311.0
33	127010.0
34	139741.5
35	151705.5
36	165006.5
37	176872.5
38	184460.5
39	191044.0
40	200134.5
41	207590.0
42	211736.5
43	216478.0
44	221563.5
45	225685.0
46	228738.0
47	231709.5
48	232885.0
49	229891.5
50	224193.5
51	218837.5
52	209332.5
53	196949.5
54	185410.0
55	172537.5
56	158286.5
57	143071.5
58	127727.0
59	111538.5
60	95472.0
61	79754.5
62	64322.5
63	51115.5
64	39715.0
65	30021.0
66	23059.0
67	17739.0
68	13135.5
69	9686.5
70	7152.0
71	5145.5
72	3617.0
73	2533.0
74	1768.0
75	1228.0
76	824.0
77	509.5
78	336.5
79	237.5
80	154.0
81	95.5
82	68.5
83	47.0
84	34.5
85	23.0
86	14.5
87	11.5
88	8.0
89	4.5
90	2.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.008213271145855194
3	0.002596701650328925
4	4.42401021907891E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	1.5387861631578815E-4
16-17	3.846965407894704E-4
18-19	0.0015387861631578816
20-21	6.732452991368323E-5
22-23	0.0017313635546048018
24-25	3.0787469384507496E-4
26-27	0.0025988041265929463
28-29	0.005750388632839804
30-31	0.0029717389555032267
32-33	0.002051375539223897
34-35	0.0023799492866806286
36-37	0.002989416293999128
38-39	0.0
40-41	8.746685453486816E-4
42-43	0.006532075803385121
44-45	0.003843920986023544
46-47	6.262120025335922E-4
48-49	0.01589503707075868
50-51	0.0105808531109652
52-53	0.014805963808234093
54-55	0.013249980860542234
56-57	0.01155080725255954
58-59	0.017645558808665158
60-61	0.008797966177499748
62-63	0.020513056334938988
64-65	0.015446400988569663
66-67	0.032394456537630095
68-69	0.022795720589596204
70-71	0.021427437953076285
72-73	0.019610217729996316
74-75	0.010647795796499033
76-77	0.013142407817692672
78-79	0.013103164031676804
80-81	0.010081302137643378
82-83	0.004755181176925432
84-85	0.003252145530889809
86-87	0.013530006718394084
88-89	0.0012727978059132292
90-91	1.9299720664114417E-4
92-93	0.001138186843065955
94-95	0.0028425294664787535
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	130.0
20-21	391.0
22-23	890.0
24-25	2165.0
26-27	3055.0
28-29	6858.0
30-31	13954.0
32-33	18601.0
34-35	25723.0
36-37	38831.0
38-39	46510.0
40-41	46731.0
42-43	50880.0
44-45	58736.0
46-47	58577.0
48-49	55315.0
50-51	51746.0
52-53	49960.0
54-55	54241.0
56-57	56607.0
58-59	55432.0
60-61	54406.0
62-63	52586.0
64-65	55804.0
66-67	57885.0
68-69	58773.0
70-71	57189.0
72-73	54026.0
74-75	55016.0
76-77	55724.0
78-79	59060.0
80-81	59339.0
82-83	56835.0
84-85	57272.0
86-87	61045.0
88-89	64596.0
90-91	68099.0
92-93	72620.0
94-95	88161.0
96-97	189508.0
98-99	1053601.0
100-101	2172025.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.95513840174276
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.76841899523731	80.71503993635216
2	5.990222701191858	10.537417334811824
3	1.305076309398417	3.4436450225392026
4	0.4393964697867534	1.5458870925332429
5	0.1849772875070055	0.8134851461928815
6	0.099946505486107	0.5274485233680649
7	0.0628945304619537	0.38723279920456355
8	0.03715972311014079	0.26147108712982975
9	0.024248509891636096	0.19195029391993929
>10	0.0843964573081142	1.1302929632397802
>50	0.0019080931818408844	0.11497112909157413
>100	0.001191522897913818	0.2159662807315
>500	1.303156335101079E-4	0.0827837859045009
>1k	3.257890746263681E-5	0.032408604980996375
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	5.770448111842056E-5	0.0	0.0	0.0
3	3.846965407894704E-5	5.770448111842056E-5	0.0	0.0	0.0
4	3.846965407894704E-5	1.1540896223684111E-4	0.0	0.0	0.0
5	3.846965407894704E-5	1.1540896223684111E-4	0.0	0.0	0.0
6	3.846965407894704E-5	1.1540896223684111E-4	0.0	0.0	1.923482703947352E-5
7	3.846965407894704E-5	1.3464378927631463E-4	0.0	1.923482703947352E-5	1.923482703947352E-5
8	3.846965407894704E-5	1.5387861631578815E-4	0.0	1.923482703947352E-5	1.923482703947352E-5
9	3.846965407894704E-5	2.6928757855262925E-4	0.0	1.923482703947352E-5	3.846965407894704E-5
10-11	3.846965407894704E-5	2.6928757855262925E-4	0.0	1.923482703947352E-5	3.846965407894704E-5
12-13	3.846965407894704E-5	2.6928757855262925E-4	0.0	1.923482703947352E-5	3.846965407894704E-5
14-15	3.846965407894704E-5	3.7507912726973366E-4	0.0	1.923482703947352E-5	3.846965407894704E-5
16-17	3.846965407894704E-5	4.039313678289439E-4	0.0	1.923482703947352E-5	3.846965407894704E-5
18-19	3.846965407894704E-5	4.3278360838815415E-4	0.0	3.846965407894704E-5	3.846965407894704E-5
20-21	3.846965407894704E-5	4.6163584894736445E-4	0.0	3.846965407894704E-5	3.846965407894704E-5
22-23	3.846965407894704E-5	5.289577435855218E-4	0.0	9.61741351973676E-5	4.80870675986838E-5
24-25	5.770448111842056E-5	6.251318787828895E-4	0.0	1.634960298355249E-4	5.770448111842056E-5
26-27	7.693930815789408E-5	6.732189463815732E-4	0.0	3.1737464615131305E-4	5.770448111842056E-5
28-29	8.655672167763084E-5	6.732189463815732E-4	0.0	7.309234274999938E-4	5.770448111842056E-5
30-31	9.61741351973676E-5	6.924537734210467E-4	0.0	0.0022120051095394547	5.770448111842056E-5
32-33	9.61741351973676E-5	6.924537734210467E-4	0.0	0.004722150038190749	5.770448111842056E-5
34-35	9.61741351973676E-5	9.136542843749922E-4	0.0	0.008328680108092033	5.770448111842056E-5
36-37	9.61741351973676E-5	0.0011540896223684112	0.0	0.013589405303388041	5.770448111842056E-5
38-39	9.61741351973676E-5	0.0011925592764473582	0.0	0.02178344162220376	5.770448111842056E-5
40-41	1.1540896223684111E-4	0.0013560553062828833	0.0	0.03028523517365106	5.770448111842056E-5
42-43	1.1540896223684111E-4	0.0013849075468420934	0.0	0.03895052475493388	6.732189463815731E-5
44-45	1.442612027960514E-4	0.0015772558172368286	0.0	0.049087278604736426	7.693930815789408E-5
46-47	1.5387861631578815E-4	0.0017311344335526168	0.0	0.05991648622796002	8.655672167763084E-5
48-49	1.7311344335526168E-4	0.001759986674111827	0.0	0.07096689436213754	1.3464378927631463E-4
50-51	1.7311344335526168E-4	0.0018753956363486682	0.0	0.0815749014744072	1.3464378927631463E-4
52-53	1.7311344335526168E-4	0.001885013049868405	0.0	0.09136542843749923	1.3464378927631463E-4
54-55	1.923482703947352E-4	0.001885013049868405	0.0	0.10066546731108467	1.3464378927631463E-4
56-57	2.1158309743420873E-4	0.001885013049868405	0.0	0.10876332949470302	1.3464378927631463E-4
58-59	2.1158309743420873E-4	0.0018946304633881417	0.0	0.11693813098647926	1.3464378927631463E-4
60-61	2.1158309743420873E-4	0.0019042478769078785	0.0	0.12493020162138052	1.3464378927631463E-4
62-63	2.1158309743420873E-4	0.0019427175309868255	0.0	0.1317585652203936	1.5387861631578815E-4
64-65	2.2120051095394548E-4	0.0020581264932236667	0.0	0.13886583381147907	1.5387861631578815E-4
66-67	2.3081792447368223E-4	0.002086978733782877	0.0	0.1462520073946369	1.5387861631578815E-4
68-69	2.3081792447368223E-4	0.0021254483878618237	0.0	0.15386899890226843	1.5387861631578815E-4
70-71	2.3081792447368223E-4	0.0021350658013815607	0.0	0.16050501423088678	1.5387861631578815E-4
72-73	2.3081792447368223E-4	0.0021543006284210344	0.0	0.16599655735065647	1.5387861631578815E-4
74-75	2.3081792447368223E-4	0.0022504747636184017	0.0	0.17005510585598538	1.5387861631578815E-4
76-77	2.40435337993419E-4	0.002365883725855243	0.0	0.1733057916256564	1.5387861631578815E-4
78-79	2.6928757855262925E-4	0.00240435337993419	0.0	0.17607560671934058	1.5387861631578815E-4
80-81	2.6928757855262925E-4	0.002413970793453927	0.0	0.17703734807131427	1.5387861631578815E-4
82-83	2.6928757855262925E-4	0.0024235882069736634	0.0	0.17750860133378138	1.5387861631578815E-4
84-85	2.6928757855262925E-4	0.002442823034013137	0.0	0.1777201844312156	1.5387861631578815E-4
86-87	2.7890499207236606E-4	0.002481292688092084	0.0	0.17783559339345242	1.5387861631578815E-4
88	2.885224055921028E-4	0.002577466823289452	0.0	0.17784521080697216	1.5387861631578815E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5210	0.0	42.957844	1
GTATCAA	8895	0.0	33.225906	1
TCAACGC	10335	0.0	27.839664	4
ATCAACG	10375	0.0	27.773172	3
CAACGCA	10515	0.0	27.284876	5
AACGCAG	11065	0.0	25.928646	6
TATCAAC	11235	0.0	25.685446	2
TACATGG	14875	0.0	22.293543	2
ACGCAGA	13325	0.0	21.56175	7
GTACATG	15850	0.0	21.516993	1
ACATGGG	15330	0.0	20.881762	3
CGCAGAG	13910	0.0	20.625483	8
CATGGGG	11095	0.0	18.470562	4
GTGGTAT	3145	0.0	18.116808	1
GCAGAGT	16080	0.0	17.689137	9
TGGTATC	3130	0.0	17.2869	2
GAGTACT	8395	0.0	16.868774	12-13
ACCGAAC	695	0.0	16.512255	8
GGACCGA	810	0.0	16.191914	6
AGAGTAC	14615	0.0	16.041117	10-11
>>END_MODULE