FastQCFastQC Report
Fri 27 May 2016
SRR2140301_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140301_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8268119
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA110850.045.227451
GTATCAA192350.034.4665681
ATCAACG235250.027.5478523
TCAACGC238500.027.2751834
CAACGCA238550.027.2003425
TATCAAC241450.027.116122
AACGCAG246350.026.4729886
ACGCAGA285650.022.6864957
CGCAGAG288600.022.426038
GTACATG281300.021.7651161
TACATGG283500.021.334442
ACATGGG291200.020.31543
GCAGAGT332050.019.317699
GTGGTAT61450.018.8533551
GAGTACT175100.018.57631712-13
CATGGGG235500.018.0649954
TGGTATC61850.017.464892
CAGAGTA326400.017.22082910-11
AGAGTAC303950.016.89915510-11
GTACTTT201150.016.17112214-15
Produced by FastQC (version 0.11.5)