##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140301_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8268119
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.954700095632393	31.0	31.0	34.0	30.0	34.0
2	32.060948808308154	33.0	31.0	34.0	30.0	34.0
3	31.78409708423403	31.0	31.0	34.0	30.0	34.0
4	35.86561973769366	37.0	35.0	37.0	35.0	37.0
5	35.7981330457387	37.0	35.0	37.0	35.0	37.0
6	35.847940988754516	37.0	35.0	37.0	35.0	37.0
7	35.82957562657238	37.0	35.0	37.0	35.0	37.0
8	35.82778223196836	37.0	35.0	37.0	35.0	37.0
9	37.59346751056679	39.0	37.0	39.0	35.0	39.0
10-11	37.498920794439464	39.0	37.0	39.0	34.5	39.0
12-13	37.446208200438335	39.0	37.0	39.0	34.0	39.0
14-15	38.67019820348497	40.0	38.0	41.0	34.0	41.0
16-17	38.60684401131624	40.0	38.0	41.0	34.0	41.0
18-19	38.610688114188974	40.0	38.0	41.0	34.0	41.0
20-21	38.67430061794134	40.0	38.0	41.0	34.0	41.0
22-23	38.62899154536752	40.0	38.0	41.0	34.0	41.0
24-25	38.52698853939861	40.0	38.0	41.0	34.0	41.0
26-27	38.3637250300327	40.0	38.0	41.0	34.0	41.0
28-29	38.229640476314806	40.0	38.0	41.0	34.0	41.0
30-31	38.09232372464752	40.0	37.5	41.0	33.0	41.0
32-33	37.89024412255306	40.0	37.0	41.0	33.0	41.0
34-35	37.9812946245383	40.0	37.0	41.0	33.0	41.0
36-37	37.93909361028308	40.0	37.0	41.0	33.0	41.0
38-39	37.89567108999151	40.0	37.0	41.0	33.0	41.0
40-41	37.82915231171439	40.0	37.0	41.0	33.0	41.0
42-43	37.86035235655077	40.0	37.0	41.0	33.0	41.0
44-45	37.8801058553587	40.0	37.0	41.0	33.0	41.0
46-47	37.818652958906654	40.0	37.0	41.0	33.0	41.0
48-49	37.71132988623248	40.0	37.0	41.0	32.5	41.0
50-51	37.59677136678106	40.0	36.0	41.0	32.0	41.0
52-53	37.31424455894966	39.0	36.0	41.0	31.0	41.0
54-55	37.23351381466669	39.0	36.0	41.0	31.0	41.0
56-57	37.04657101633227	39.0	35.0	41.0	31.0	41.0
58-59	36.85468965246278	39.0	35.0	40.0	31.0	41.0
60-61	36.67895482809803	38.0	35.0	40.0	31.0	41.0
62-63	36.40851189952052	38.0	35.0	40.0	31.0	41.0
64-65	36.031230633270106	37.0	34.5	40.0	30.0	41.0
66-67	35.774319428292884	37.0	34.0	40.0	30.0	41.0
68-69	35.47363617384934	36.0	34.0	39.0	29.0	41.0
70-71	35.19891198165993	36.0	34.0	39.0	29.0	40.5
72-73	34.90825565660847	35.5	34.0	38.5	29.0	40.0
74-75	34.59247790606248	35.0	34.0	37.5	29.0	40.0
76-77	33.39217535734325	34.5	31.5	36.0	27.5	39.0
78-79	34.03709284690876	35.0	33.0	36.5	29.0	39.0
80-81	33.99393944436912	35.0	33.0	36.0	29.0	39.0
82-83	33.74690508337742	35.0	33.0	36.0	29.0	37.0
84-85	33.536161122274365	35.0	33.0	35.5	29.0	37.0
86-87	33.360361033591076	35.0	33.0	35.0	29.0	36.5
88-89	33.20759711587683	35.0	33.0	35.0	29.0	36.0
90-91	33.08808411238372	35.0	33.0	35.0	29.0	36.0
92-93	33.07342818128913	35.0	33.0	35.0	29.0	36.0
94-95	33.08145720754424	35.0	33.0	35.0	29.0	35.5
96-97	33.084294043452275	35.0	33.0	35.0	29.0	35.0
98-99	33.20374347215487	34.0	33.0	35.0	29.0	35.0
100	32.01866381972686	34.0	31.0	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	2.0
19	13.0
20	57.0
21	328.0
22	1311.0
23	4575.0
24	12500.0
25	26636.0
26	48078.0
27	76150.0
28	109769.0
29	146255.0
30	185760.0
31	230760.0
32	286555.0
33	362794.0
34	485681.0
35	670690.0
36	1040050.0
37	1714919.0
38	1897691.0
39	935937.0
40	31606.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.023439672312406	20.09342149042606	13.869696360199942	24.01344247706159
2	15.327882415382883	20.809664279642302	40.26536031069216	23.597092994282658
3	18.572266233054673	25.730008802704045	30.143661387866395	25.554063576374887
4	13.1705177787174	17.395025306939164	37.55255144841226	31.881905465931183
5	13.562867201113093	37.65463462729552	35.08317913663313	13.699319034958254
6	31.12187911180282	36.90486312545816	18.07573161440952	13.897526148329506
7	27.480204385060254	32.16135374926268	22.354758077381327	18.003683788295742
8	25.465816348313325	36.104681125174906	20.44918560073942	17.980316925772357
9	25.959725543379335	16.418075259923086	20.832513416896877	36.7896857798007
10-11	25.214894705797047	26.250202736559547	27.838913542487713	20.695989015155682
12-13	25.656234507510113	23.694966170661065	28.394940856559998	22.253858465268824
14-15	22.729734541445275	25.104104832689657	25.905878346049665	26.260282279815407
16-17	22.317938176349628	28.337454393985173	26.574491497318974	22.77011593234623
18-19	22.35330637025055	27.55160682399546	28.309664589434057	21.785422216319926
20-21	23.13248385302015	26.586619173177873	28.218582936210357	22.06231403759162
22-23	23.009621653922874	26.54902700813014	28.056054541253715	22.385296796693265
24-25	22.76954071946008	26.670276733983272	28.140406169476385	22.419776377080264
26-27	22.716242574744637	26.834855638545633	28.0286404738896	22.420261312820134
28-29	22.47231340547127	27.005795052472664	28.040127804832714	22.481763737223346
30-31	22.80599976886988	26.739023504379333	28.03056622994728	22.42441049680351
32-33	22.555039489666566	26.95649971126955	27.944242231299672	22.54421856776421
34-35	22.58389317145263	27.055837470336787	28.07822712255945	22.282042235651137
36-37	22.78094807407729	26.953070169557375	27.82867802724477	22.43730372912057
38-39	22.601154900474008	26.953227349599434	27.951520530461227	22.49409721946533
40-41	22.71384443092826	26.824254566456162	27.853684039738418	22.608216962877155
42-43	22.73085580244009	26.94671227843162	27.65359344435232	22.668838474775974
44-45	22.707443406214345	27.105050443173397	27.489014902593805	22.698491248018456
46-47	22.828663532909562	27.07918034130393	27.192802448623738	22.89935367716277
48-49	22.852961110457777	27.183285217820284	27.111808048093454	22.851945623628477
50-51	22.703237543738283	27.253529233858547	27.091141355746696	22.952091866656474
52-53	22.833368787603803	27.223011197772394	27.03000568852004	22.91361432610376
54-55	22.802668333874106	27.14265429975767	27.03861152672277	23.016065839645453
56-57	22.91432800729279	27.23601340509533	26.928335096778145	22.921323490833732
58-59	22.755593828997124	27.4257774208374	27.06738766534555	22.751241084819924
60-61	22.984694836507405	27.412107768764766	26.873779599664672	22.72941779506316
62-63	22.921686068090587	27.509102487748354	26.74839445689214	22.82081698726892
64-65	22.79661574558324	27.468801194735228	26.82376762388416	22.910815435797378
66-67	22.90048670931429	27.3034091344586	26.778723333485182	23.017380822741927
68-69	22.798262482550527	27.250241016860567	26.975002324156517	22.976494176432393
70-71	22.78116705409959	27.125275380759945	26.942451609919427	23.151105955221034
72-73	22.927643000728423	27.143972972930396	26.91609953341415	23.012284492927026
74-75	22.714427588584826	27.286923079024373	26.99274312092846	23.005906211462342
76-77	22.897949038215003	27.15917761879016	26.857637637079595	23.08523570591524
78-79	22.80037200144192	27.111948940990754	26.902377452415156	23.185301605152173
80-81	22.77522458998302	27.206291788522165	27.008151147107018	23.0103324743878
82-83	22.842307794857923	27.14185402788637	27.09370884289183	22.92212933436388
84-85	22.964277763180206	27.099243248910465	27.015522037228557	22.92095695068077
86-87	22.777503357241965	27.143521776151434	27.104257899188973	22.974716967417628
88-89	22.80853247274387	26.988276339349394	27.117161580273947	23.086029607632792
90-91	22.91964436815358	27.010406191005952	27.047791574455456	23.02215786638501
92-93	22.955646455069765	26.963699688941645	27.08699231743689	22.9936615385517
94-95	22.721918980963963	27.02334311745929	27.238274923795863	23.016462977780883
96-97	22.764579208843365	27.184045221357326	27.234787530039196	22.816588039760113
98-99	23.879340159999618	29.137354493654705	27.43193281851579	19.551372527829887
100	22.927131803905894	30.048868054141426	21.07805031377456	25.94594982817811
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1213.0
1	1150.5
2	1963.5
3	2967.5
4	3989.0
5	6770.0
6	9253.5
7	10870.5
8	11880.5
9	11522.0
10	10030.5
11	8408.0
12	8773.5
13	11937.0
14	19754.0
15	30892.0
16	40325.5
17	45845.5
18	47291.5
19	45374.5
20	41460.5
21	36891.0
22	33771.0
23	34393.0
24	39367.0
25	47755.0
26	58453.0
27	70975.5
28	85403.5
29	103029.0
30	120829.0
31	137265.5
32	154771.0
33	171608.5
34	188603.5
35	206038.5
36	223938.5
37	241999.5
38	256392.5
39	266936.5
40	277233.0
41	288641.5
42	300143.5
43	312964.5
44	328672.5
45	346780.0
46	360838.0
47	370979.0
48	376033.5
49	372692.5
50	369437.5
51	368886.5
52	362770.0
53	349911.5
54	336740.0
55	318719.5
56	297620.5
57	273504.5
58	244904.5
59	214552.5
60	179788.0
61	146111.0
62	118026.0
63	93197.0
64	70584.0
65	52776.5
66	39955.0
67	30893.0
68	23995.0
69	17948.5
70	13135.0
71	9635.0
72	7016.5
73	4945.0
74	3394.0
75	2269.5
76	1493.5
77	996.5
78	667.5
79	471.5
80	336.0
81	224.5
82	151.5
83	106.0
84	72.5
85	48.5
86	35.0
87	28.0
88	19.5
89	12.5
90	7.5
91	4.0
92	2.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.007776859525122946
3	0.00247940311454153
4	3.7493412951603624E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	1.3304114273149674E-4
16-17	2.539876361237665E-4
18-19	0.0017839607775359788
20-21	7.861819214080542E-5
22-23	0.0018205086162374489
24-25	2.722364880863868E-4
26-27	0.0026328389830123783
28-29	0.005803136177132555
30-31	0.002961873885352174
32-33	0.0022165043716590688
34-35	0.002243989943745557
36-37	0.0028047795904554335
38-39	0.0
40-41	7.827442587900302E-4
42-43	0.0060141680138466255
44-45	0.0038876432729224686
46-47	4.5313989697411307E-4
48-49	0.01575394241590146
50-51	0.011060440605566409
52-53	0.014812984068950092
54-55	0.012703836605956517
56-57	0.010765327339723676
58-59	0.016724176603712795
60-61	0.008579535014353953
62-63	0.019285477985626878
64-65	0.014186325311011612
66-67	0.02971723949037284
68-69	0.02096791250708536
70-71	0.020622435950589445
72-73	0.019123939099478456
74-75	0.009423433088693322
76-77	0.011593689255498576
78-79	0.012250322911827136
80-81	0.009530385902653173
82-83	0.003959387540222508
84-85	0.003009310418135593
86-87	0.01337057297662832
88-89	0.0012240533987050014
90-91	2.455102939926509E-4
92-93	0.0011634496851963697
94-95	0.0026999870277496225
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	192.0
20-21	714.0
22-23	1496.0
24-25	3595.0
26-27	5449.0
28-29	12436.0
30-31	25858.0
32-33	31513.0
34-35	42937.0
36-37	63804.0
38-39	76978.0
40-41	77055.0
42-43	83193.0
44-45	95374.0
46-47	92732.0
48-49	85593.0
50-51	77871.0
52-53	72790.0
54-55	78032.0
56-57	82254.0
58-59	81181.0
60-61	79957.0
62-63	77530.0
64-65	81245.0
66-67	85132.0
68-69	84790.0
70-71	82092.0
72-73	77028.0
74-75	78124.0
76-77	77824.0
78-79	83815.0
80-81	84992.0
82-83	82968.0
84-85	84360.0
86-87	88947.0
88-89	96236.0
90-91	103174.0
92-93	112365.0
94-95	136927.0
96-97	311646.0
98-99	533948.0
100-101	4733972.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.4936743669325
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.93233429557998	71.65929017408244
2	7.76238901872071	12.651712060021564
3	2.235512945156649	5.465404919869745
4	0.8974021858192374	2.925304060293055
5	0.4414583450277852	1.7988031308129628
6	0.24815730417639642	1.2133950322996239
7	0.14629873184687914	0.8345694849397319
8	0.09236392741462446	0.6021660659182715
9	0.06310862906875805	0.4628658660365608
>10	0.17808153239305138	2.1812101631864227
>50	0.0022293756748747577	0.12154525042616089
>100	6.637091210786619E-4	0.08373379211346263
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.418929867845395E-5	0.0	0.0	0.0
3	0.0	3.628394801768092E-5	0.0	0.0	0.0
4	0.0	7.256789603536185E-5	0.0	1.2094649339226975E-5	0.0
5	0.0	7.256789603536185E-5	0.0	1.2094649339226975E-5	0.0
6	0.0	7.256789603536185E-5	0.0	1.2094649339226975E-5	0.0
7	0.0	7.256789603536185E-5	0.0	2.418929867845395E-5	0.0
8	0.0	8.466254537458883E-5	0.0	3.628394801768092E-5	0.0
9	0.0	1.3304114273149674E-4	0.0	4.83785973569079E-5	0.0
10-11	0.0	1.3304114273149674E-4	0.0	9.070987004420231E-5	0.0
12-13	0.0	1.3908846740111021E-4	0.0	9.67571947138158E-5	0.0
14-15	0.0	2.3584566211492603E-4	0.0	1.1489916872265627E-4	0.0
16-17	0.0	2.5398763612376646E-4	0.0	1.451357920707237E-4	0.0
18-19	0.0	2.842242594718339E-4	0.0	1.5723044140995068E-4	0.0
20-21	0.0	3.1446088281990135E-4	0.0	1.8141974008840462E-4	0.0
22-23	0.0	3.7493412951603624E-4	0.0	2.1165636343647206E-4	0.0
24-25	0.0	4.233127268729441E-4	0.0	2.842242594718339E-4	0.0
26-27	0.0	4.475020255513981E-4	0.0	4.293600515425576E-4	0.0
28-29	0.0	4.475020255513981E-4	0.0	9.91761245816612E-4	0.0
30-31	0.0	5.503065449348274E-4	0.0	0.002745485400004523	0.0
32-33	0.0	5.684485189436678E-4	0.0	0.005926378176221218	0.0
34-35	0.0	6.71253038327097E-4	0.0	0.009857139211469985	0.0
36-37	0.0	7.982468563889804E-4	0.0	0.015989126426458063	0.0
38-39	0.0	8.889567264331827E-4	0.0	0.025586230677134666	0.0
40-41	0.0	0.0010703764665215875	0.0	0.03554617440798808	0.0
42-43	0.0	0.0011368970378873356	0.0	0.04433898447760609	0.0
44-45	0.0	0.0012336542326011515	0.0	0.05383933153356888	0.0
46-47	0.0	0.0013304114273149674	0.0	0.06461566409482011	0.0
48-49	0.0	0.0013485534013238077	0.0	0.07594835052567579	0.0
50-51	0.0	0.0014815945440553044	0.0	0.08674887238560547	0.0
52-53	0.0	0.001487641868724918	0.0	0.09710189221998378	0.0
54-55	0.0	0.0014936891933945316	0.0	0.10701345735348028	0.0
56-57	0.0	0.001499736518064145	0.0	0.11555832711164413	0.0
58-59	0.0	0.001511831167403372	0.0	0.12390968248038037	0.0
60-61	0.0	0.0015904463881083472	0.0	0.13233965306982157	0.0
62-63	0.0	0.0016267303361260283	0.0	0.1396569159200539	0.0
64-65	0.0	0.0017174402061702304	0.0	0.14679880635486742	0.0
66-67	0.0	0.0017355821801790712	0.0	0.15429144162051853	0.0
68-69	0.0	0.0017779134528663654	0.0	0.1617659349121608	0.0
70-71	0.0	0.0018141974008840463	0.0	0.16765602914036432	0.0
72-73	0.0	0.0018323393748928868	0.0	0.17299581682363302	0.0
74-75	0.0	0.0018444340242321135	0.0	0.1768781992615249	0.0
76-77	0.0	0.001874670647580181	0.0	0.180694061128051	0.0
78-79	0.0	0.0018988599462586352	0.0	0.18314322761924445	0.0
80-81	0.0	0.0019109545955978621	0.0	0.18394147447563344	0.0
82-83	0.0	0.001923049244937089	0.0	0.1844675917218898	0.0
84-85	0.0	0.001935143894276316	0.0	0.18470948470867438	0.0
86-87	0.0	0.00195933319295477	0.0	0.18488485712409314	0.0
88	0.0	0.001983522491633224	0.0	0.18489090444876277	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11085	0.0	45.22745	1
GTATCAA	19235	0.0	34.466568	1
ATCAACG	23525	0.0	27.547852	3
TCAACGC	23850	0.0	27.275183	4
CAACGCA	23855	0.0	27.200342	5
TATCAAC	24145	0.0	27.11612	2
AACGCAG	24635	0.0	26.472988	6
ACGCAGA	28565	0.0	22.686495	7
CGCAGAG	28860	0.0	22.42603	8
GTACATG	28130	0.0	21.765116	1
TACATGG	28350	0.0	21.33444	2
ACATGGG	29120	0.0	20.3154	3
GCAGAGT	33205	0.0	19.31769	9
GTGGTAT	6145	0.0	18.853355	1
GAGTACT	17510	0.0	18.576317	12-13
CATGGGG	23550	0.0	18.064995	4
TGGTATC	6185	0.0	17.46489	2
CAGAGTA	32640	0.0	17.220829	10-11
AGAGTAC	30395	0.0	16.899155	10-11
GTACTTT	20115	0.0	16.171122	14-15
>>END_MODULE