FastQCFastQC Report
Fri 27 May 2016
SRR2140300_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140300_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8437844
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA115400.048.0184331
GTATCAA204250.034.5819131
ATCAACG252700.027.5126253
TCAACGC253350.027.4412234
CAACGCA255650.027.2103045
TATCAAC260150.026.9314962
AACGCAG263600.026.5289616
GTGGTAT56150.022.8188571
ACGCAGA305950.022.790117
CGCAGAG309500.022.5023388
GTACATG315800.021.4620651
TACATGG316400.021.0473632
TGGTATC56000.020.9854582
ACATGGG326800.019.9758663
GCAGAGT354850.019.4080759
GAGTACT183600.018.4225412-13
CATGGGG246750.018.4197124
CAGAGTA347300.017.7448910-11
AGAGTAC324850.017.269410-11
ATGGGGG168700.016.397195
Produced by FastQC (version 0.11.5)