##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140300_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8437844
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.97831353601702	31.0	31.0	34.0	30.0	34.0
2	32.08149724029029	33.0	31.0	34.0	30.0	34.0
3	31.81020329363757	31.0	31.0	34.0	30.0	34.0
4	35.88401492134721	37.0	35.0	37.0	35.0	37.0
5	35.82182249399254	37.0	35.0	37.0	35.0	37.0
6	35.86881447440839	37.0	35.0	37.0	35.0	37.0
7	35.85252204236058	37.0	35.0	37.0	35.0	37.0
8	35.85132588372101	37.0	35.0	37.0	35.0	37.0
9	37.62177838319836	39.0	37.0	39.0	35.0	39.0
10-11	37.52947589455316	39.0	37.0	39.0	35.0	39.0
12-13	37.473645637440086	39.0	37.0	39.0	34.0	39.0
14-15	38.70200663818862	40.0	38.0	41.0	34.0	41.0
16-17	38.64160975244387	40.0	38.0	41.0	34.0	41.0
18-19	38.65151844475911	40.0	38.0	41.0	34.0	41.0
20-21	38.715979281137386	40.0	38.0	41.0	34.0	41.0
22-23	38.67363930878669	40.0	38.0	41.0	34.0	41.0
24-25	38.57798167215596	40.0	38.0	41.0	34.0	41.0
26-27	38.420274276441646	40.0	38.0	41.0	34.0	41.0
28-29	38.29048600375653	40.0	38.0	41.0	34.0	41.0
30-31	38.16095478447859	40.0	38.0	41.0	33.5	41.0
32-33	37.96373078073671	40.0	37.0	41.0	33.0	41.0
34-35	38.04472254129188	40.0	37.0	41.0	33.0	41.0
36-37	38.002272034385086	40.0	37.0	41.0	33.0	41.0
38-39	37.96775559086943	40.0	37.0	41.0	33.0	41.0
40-41	37.90223547431604	40.0	37.0	41.0	33.0	41.0
42-43	37.93737788338262	40.0	37.0	41.0	33.0	41.0
44-45	37.96588190881804	40.0	37.0	41.0	33.0	41.0
46-47	37.90705836401545	40.0	37.0	41.0	33.0	41.0
48-49	37.81040829573206	40.0	37.0	41.0	33.0	41.0
50-51	37.70643032561924	40.0	36.5	41.0	32.5	41.0
52-53	37.42953478211584	39.0	36.0	41.0	32.0	41.0
54-55	37.356523738548525	39.0	36.0	41.0	32.0	41.0
56-57	37.176647413824995	39.0	35.5	41.0	31.5	41.0
58-59	36.99371385310321	39.0	35.0	40.5	31.0	41.0
60-61	36.82539860699767	38.5	35.0	40.0	31.0	41.0
62-63	36.56497266660087	38.0	35.0	40.0	31.0	41.0
64-65	36.19872238625584	37.5	35.0	40.0	30.0	41.0
66-67	35.94613345148903	37.0	34.0	40.0	30.0	41.0
68-69	35.65184064029205	36.5	34.0	39.0	30.0	41.0
70-71	35.37870310956758	36.0	34.0	39.0	30.0	41.0
72-73	35.08711202381261	36.0	34.0	39.0	29.0	40.0
74-75	34.7707417381413	35.0	34.0	38.0	29.0	40.0
76-77	33.55604806946906	34.5	32.0	36.5	28.0	39.0
78-79	34.18675770179358	35.0	33.0	37.0	29.0	39.0
80-81	34.13543949307771	35.0	34.0	36.0	29.0	39.0
82-83	33.87933257412295	35.0	33.0	36.0	29.0	37.5
84-85	33.66066136338027	35.0	33.0	36.0	29.0	37.0
86-87	33.47107900527111	35.0	33.0	35.0	29.0	37.0
88-89	33.31195784958668	35.0	33.0	35.0	29.0	36.0
90-91	33.191750790627154	35.0	33.0	35.0	29.0	36.0
92-93	33.17406184703996	35.0	33.0	35.0	29.0	36.0
94-95	33.17093560458457	35.0	33.0	35.0	29.0	35.5
96-97	33.162936262494725	35.0	33.0	35.0	29.0	35.0
98-99	33.277390070285655	35.0	33.0	35.0	29.0	35.0
100	32.14143798715743	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	3.0
17	0.0
18	2.0
19	14.0
20	61.0
21	301.0
22	1314.0
23	4532.0
24	11748.0
25	24734.0
26	45302.0
27	72291.0
28	104025.0
29	139315.0
30	179389.0
31	223774.0
32	279030.0
33	355797.0
34	482714.0
35	661616.0
36	1025255.0
37	1715863.0
38	2008479.0
39	1064773.0
40	37512.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.072880228646085	20.16375273114791	13.914360113792101	23.849006926413903
2	15.247576292402067	21.00802199598278	40.39513453374274	23.34926717787241
3	18.627156663834512	25.93943771157459	30.254845349290743	25.178560275300153
4	12.9738686841261	17.540849180361676	38.07510648205156	31.41017565346067
5	13.324138251430103	37.968502380465914	35.20850823978258	13.498851128321403
6	30.684023075088852	37.15428965029455	18.364288318200717	13.79739895641588
7	27.21233054320511	32.48231420253799	22.520551458405723	17.784803795851168
8	25.30787485523553	36.06718730519313	20.73533239059646	17.88960544897488
9	26.103101692802095	16.310896480190912	20.94992512305276	36.63607670395423
10-11	25.010174400000757	26.375013569817124	28.184539794762735	20.43027223541938
12-13	25.592378811459422	23.881550784773932	28.509285073295977	22.016785330470675
14-15	22.657915999162107	25.385156673825893	26.151443513485244	25.80548381352676
16-17	22.051591646396325	28.49503950635588	27.005199625329745	22.448169221918057
18-19	22.07064521208005	27.677108870386874	28.66779963754334	21.584446279989738
20-21	22.969404294435193	26.728949156174252	28.525584045746122	21.776062503644432
22-23	22.80825837863152	26.671700687795212	28.369571588444654	22.150469345128613
24-25	22.533869814100594	26.849838459425403	28.446239431929182	22.170052294544824
26-27	22.453394962352	26.996840876227907	28.434011783537006	22.115752377883087
28-29	22.29863374620053	27.248587954295	28.282520979151325	22.170257320353144
30-31	22.53274842137241	27.09085731733512	28.192471781658195	22.183922479634273
32-33	22.30598625411541	27.13090791885908	28.3328892738532	22.23021655317231
34-35	22.43075618270805	27.1629845160101	28.35002224061497	22.05623706066689
36-37	22.46463730314239	27.30675911385301	28.10023555511204	22.128368027892563
38-39	22.37749648917403	27.169484468632437	28.236955949951138	22.216063092242397
40-41	22.565864248314618	27.128637886465622	28.042449109917456	22.263048755302304
42-43	22.412806175291312	27.239768909923654	27.842265675355215	22.50515923942982
44-45	22.580756025447624	27.315818700495264	27.723831753482475	22.379593520574637
46-47	22.70890976552522	27.238365945939453	27.40695837875114	22.645765909784192
48-49	22.58216220298372	27.44803927448577	27.307964783121268	22.661833739409243
50-51	22.58328651262901	27.551985072250908	27.227708140012925	22.637020275107165
52-53	22.61204198673315	27.553876450196178	27.173155851888016	22.66092571118266
54-55	22.631650709958652	27.392350986232643	27.15666691980956	22.819331383999145
56-57	22.687569940248554	27.480977675079437	27.15306892966743	22.67838345500458
58-59	22.666688424611227	27.620245611997383	27.11345463067455	22.599611332716844
60-61	22.77126189233895	27.59930172404223	27.02397340112568	22.605462982493144
62-63	22.64194947322426	27.733766301235917	26.99047150479795	22.63381272074188
64-65	22.62662454033364	27.689447237108766	27.03290224361854	22.65102597893906
66-67	22.703470203033728	27.605147448178784	26.908059422793883	22.783322925993605
68-69	22.713231809741377	27.565331915835223	27.02371866877349	22.69771760564991
70-71	22.58790296701574	27.373881239661884	27.119374979150322	22.91884081417205
72-73	22.672747690179694	27.466674720738393	27.10574415548386	22.754833433598055
74-75	22.62620570059548	27.45008283544647	27.093219451907796	22.83049201205025
76-77	22.612539194566043	27.35200361854967	27.117917187344275	22.917539999540015
78-79	22.620031324679683	27.33155215652395	27.062060467819176	22.986356050977186
80-81	22.549350692123888	27.409881910069988	27.129111625295455	22.911655772510674
82-83	22.590419710392187	27.39265841734053	27.227316458035517	22.78960541423177
84-85	22.75697527538494	27.299543874570936	27.17111115903007	22.772369691014056
86-87	22.65464713516107	27.260548215819767	27.21078266266524	22.87402198635392
88-89	22.651688469591935	27.218209385975044	27.23366251535493	22.896439629078092
90-91	22.70954142147057	27.18401856439695	27.282758843534666	22.823681170597812
92-93	22.789024803423104	27.112014288279752	27.24917435840438	22.849786549892766
94-95	22.608483401319933	27.262702439779346	27.296989796482386	22.83182436241833
96-97	22.59682676041771	27.40290489605054	27.3748243373211	22.625444006210653
98-99	23.773577635357995	29.347009146302206	27.56153212416163	19.317881094178173
100	30.58652190274381	40.842947544793695	28.570530552462493	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1120.0
1	1105.0
2	1935.0
3	2912.5
4	3945.0
5	6968.5
6	9604.5
7	11021.5
8	11881.0
9	11525.5
10	10290.5
11	8830.5
12	9115.5
13	12446.0
14	20963.0
15	33425.0
16	44427.0
17	51636.0
18	54418.5
19	52891.0
20	48494.5
21	42910.0
22	38525.0
23	37922.5
24	41853.5
25	49948.0
26	61999.0
27	76641.0
28	92136.5
29	110019.0
30	128226.5
31	145776.5
32	164714.5
33	184022.0
34	202686.5
35	220841.5
36	240886.5
37	260458.5
38	276617.0
39	290849.5
40	305258.5
41	320076.5
42	333606.5
43	347324.0
44	361512.0
45	374569.5
46	384145.5
47	390637.0
48	394399.5
49	393132.5
50	392414.5
51	390028.5
52	378231.0
53	359347.5
54	337450.5
55	310020.0
56	278037.0
57	247095.0
58	218204.5
59	187051.5
60	155249.5
61	127196.5
62	102682.0
63	81317.5
64	62389.0
65	47528.5
66	36064.0
67	27730.0
68	21415.5
69	15969.0
70	11795.0
71	8565.5
72	6013.0
73	4105.0
74	2845.5
75	1949.0
76	1306.5
77	847.0
78	567.0
79	375.5
80	246.0
81	163.0
82	117.5
83	88.5
84	64.0
85	46.5
86	34.5
87	24.5
88	16.5
89	11.5
90	8.0
91	5.5
92	5.0
93	4.0
94	2.5
95	1.5
96	1.0
97	0.5
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.007904862900996986
3	0.0025717470007741317
4	4.385006406849901E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	1.3629073967236178E-4
16-17	2.903585323454665E-4
18-19	0.0017243741410720558
20-21	7.111085827250392E-5
22-23	0.0016297777824662697
24-25	2.667537508985896E-4
26-27	0.002407819792124496
28-29	0.005977403751779495
30-31	0.002902783346898492
32-33	0.0019580818844771087
34-35	0.0024527945005588655
36-37	0.0028855709920188608
38-39	0.0
40-41	7.144729114678754E-4
42-43	0.0066144511182719915
44-45	0.003934810779111452
46-47	5.817025154159157E-4
48-49	0.0161506154329945
50-51	0.011205566243801798
52-53	0.015190207834238727
54-55	0.013184461191782888
56-57	0.011481788089003029
58-59	0.017494714395702608
60-61	0.00889417883626677
62-63	0.020172829346707766
64-65	0.01484034873541412
66-67	0.03188777343981377
68-69	0.02212287560681344
70-71	0.02128058974092579
72-73	0.02008379734603867
74-75	0.009883722470219233
76-77	0.012349075478913781
78-79	0.012724031905471164
80-81	0.010305036972865451
82-83	0.0045719737370482055
84-85	0.0033722279840123965
86-87	0.013811344600615644
88-89	0.0012285463012774959
90-91	2.2958847880796303E-4
92-93	0.0011521555704556126
94-95	0.0024938853380501373
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	194.0
20-21	640.0
22-23	1497.0
24-25	3496.0
26-27	5900.0
28-29	13539.0
30-31	28761.0
32-33	35655.0
34-35	48823.0
36-37	72646.0
38-39	87866.0
40-41	87901.0
42-43	95502.0
44-45	106910.0
46-47	103285.0
48-49	93636.0
50-51	84412.0
52-53	79420.0
54-55	84653.0
56-57	87531.0
58-59	86917.0
60-61	84876.0
62-63	81575.0
64-65	84296.0
66-67	89238.0
68-69	90078.0
70-71	88304.0
72-73	82611.0
74-75	84012.0
76-77	84872.0
78-79	89306.0
80-81	91643.0
82-83	88076.0
84-85	88412.0
86-87	93888.0
88-89	100163.0
90-91	107845.0
92-93	114581.0
94-95	136374.0
96-97	306619.0
98-99	1742124.0
100-101	3499767.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.61678968416582
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.54021734549649	75.76605739398373
2	7.220943834885729	12.220261715953992
3	1.7694354702122237	4.491718471279524
4	0.6771829767233539	2.292041980763896
5	0.309864890856569	1.310988615005865
6	0.16474053458471324	0.8363889100447037
7	0.10245637819344096	0.6068670863777024
8	0.05271342932021056	0.3568352929855518
9	0.0353426942288908	0.2691526791994051
>10	0.1246026271481372	1.6167232530667592
>50	0.0017444316127282976	0.09914227597816513
>100	7.323127347348337E-4	0.12183429433718185
>500	2.307400288666667E-5	0.011988031023518062
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	1.1851368667161896E-5	1.1851368667161896E-5	0.0	0.0	1.1851368667161896E-5
3	1.1851368667161896E-5	1.1851368667161896E-5	0.0	0.0	1.1851368667161896E-5
4	1.1851368667161896E-5	1.1851368667161896E-5	0.0	0.0	1.1851368667161896E-5
5	2.3702737334323793E-5	3.5554106001485684E-5	0.0	0.0	1.1851368667161896E-5
6	2.3702737334323793E-5	3.5554106001485684E-5	0.0	0.0	1.1851368667161896E-5
7	2.3702737334323793E-5	3.5554106001485684E-5	0.0	0.0	2.3702737334323793E-5
8	3.5554106001485684E-5	3.5554106001485684E-5	0.0	0.0	3.5554106001485684E-5
9	3.5554106001485684E-5	7.110821200297137E-5	0.0	0.0	3.5554106001485684E-5
10-11	3.5554106001485684E-5	7.110821200297137E-5	0.0	5.925684333580948E-6	3.5554106001485684E-5
12-13	3.5554106001485684E-5	7.110821200297137E-5	0.0	2.370273733432379E-5	4.147979033506664E-5
14-15	5.925684333580948E-5	9.481094933729517E-5	0.0	3.5554106001485684E-5	4.7405474668647586E-5
16-17	5.925684333580948E-5	9.481094933729517E-5	0.0	3.5554106001485684E-5	4.7405474668647586E-5
18-19	7.703389633655232E-5	1.1258800233803802E-4	0.0	3.5554106001485684E-5	4.7405474668647586E-5
20-21	1.1851368667161895E-4	1.4221642400594274E-4	0.0	6.518252766939043E-5	7.703389633655232E-5
22-23	1.4221642400594274E-4	1.7777053000742844E-4	0.0	7.703389633655232E-5	8.295958067013328E-5
24-25	1.7777053000742844E-4	1.8962189867459032E-4	0.0	1.481421083395237E-4	8.295958067013328E-5
26-27	2.2517600467607601E-4	2.0147326734175222E-4	0.0	2.5480442634398076E-4	8.295958067013328E-5
28-29	2.2517600467607601E-4	2.0147326734175222E-4	0.0	7.407105416976185E-4	8.295958067013328E-5
30-31	2.2517600467607601E-4	2.844328480118855E-4	0.0	0.002405827839433865	8.295958067013328E-5
32-33	2.4295305767681885E-4	2.962842166790474E-4	0.0	0.006293076762262966	1.1851368667161896E-4
34-35	2.6665579501114267E-4	3.9109516601634254E-4	0.0	0.01050623832343902	1.1851368667161896E-4
36-37	2.844328480118855E-4	5.096088526879615E-4	0.0	0.01717855888305117	1.1851368667161896E-4
38-39	3.0220990101262834E-4	5.570143273566091E-4	0.0	0.027649243100488705	1.1851368667161896E-4
40-41	3.7331811301559973E-4	6.636766453610662E-4	0.0	0.037829568785580775	1.1851368667161896E-4
42-43	3.792437973491807E-4	6.636766453610662E-4	0.0	0.04738769761564684	1.1851368667161896E-4
44-45	3.792437973491807E-4	7.051564356961328E-4	0.0	0.05744950961406729	1.1851368667161896E-4
46-47	3.792437973491807E-4	7.466362260311995E-4	0.0	0.06869645847920393	1.1851368667161896E-4
48-49	3.792437973491807E-4	7.466362260311995E-4	0.0	0.07904862900996984	1.244393710051999E-4
50-51	3.792437973491807E-4	7.821903320326851E-4	0.0	0.08930006290706488	1.3036505533878086E-4
52-53	3.792437973491807E-4	7.88116016366266E-4	0.0	0.09970556459683302	1.3036505533878086E-4
54-55	3.792437973491807E-4	8.177444380341708E-4	0.0	0.11015254607693625	1.3036505533878086E-4
56-57	3.792437973491807E-4	8.295958067013327E-4	0.0	0.11962771532633218	1.3629073967236178E-4
58-59	3.9109516601634254E-4	8.710755970363993E-4	0.0	0.12832069424369544	1.4221642400594274E-4
60-61	4.0294653468350445E-4	9.007040187043041E-4	0.0	0.13698404473939077	1.481421083395237E-4
62-63	4.0294653468350445E-4	9.362581247057898E-4	0.0	0.14513778638239816	1.5406779267310465E-4
64-65	4.0294653468350445E-4	0.0010429204427102468	0.0	0.15222490484536097	1.5406779267310465E-4
66-67	4.0294653468350445E-4	0.0010547718113774088	0.0	0.16020680164269452	1.5406779267310465E-4
68-69	4.0294653468350445E-4	0.0010606974957109898	0.0	0.16802277927868778	1.5406779267310465E-4
70-71	4.0294653468350445E-4	0.0010784745487117326	0.0	0.17512767479465133	1.6591916134026655E-4
72-73	4.0294653468350445E-4	0.0010903259173788945	0.0	0.18011710100352651	1.6591916134026655E-4
74-75	4.088722190170854E-4	0.0010962516017124753	0.0	0.18383843076501533	1.6591916134026655E-4
76-77	4.1479790335066636E-4	0.0011140286547132183	0.0	0.187547909157837	1.7777053000742844E-4
78-79	4.1479790335066636E-4	0.0011140286547132183	0.0	0.18995373699727086	1.7777053000742844E-4
80-81	4.2664927201782827E-4	0.001131805707713961	0.0	0.1907181502763028	1.8962189867459034E-4
82-83	4.2664927201782827E-4	0.001137731392047542	0.0	0.19129886734099374	1.9554758300817127E-4
84-85	4.2664927201782827E-4	0.0011495827607147038	0.0	0.19148256355533474	2.0147326734175222E-4
86-87	4.2664927201782827E-4	0.0011732854980490276	0.0	0.1915595974516713	2.1332463600891413E-4
88	4.2664927201782827E-4	0.0011851368667161895	0.0	0.1915655231360049	2.2517600467607601E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11540	0.0	48.018433	1
GTATCAA	20425	0.0	34.581913	1
ATCAACG	25270	0.0	27.512625	3
TCAACGC	25335	0.0	27.441223	4
CAACGCA	25565	0.0	27.210304	5
TATCAAC	26015	0.0	26.931496	2
AACGCAG	26360	0.0	26.528961	6
GTGGTAT	5615	0.0	22.818857	1
ACGCAGA	30595	0.0	22.79011	7
CGCAGAG	30950	0.0	22.502338	8
GTACATG	31580	0.0	21.462065	1
TACATGG	31640	0.0	21.047363	2
TGGTATC	5600	0.0	20.985458	2
ACATGGG	32680	0.0	19.975866	3
GCAGAGT	35485	0.0	19.408075	9
GAGTACT	18360	0.0	18.42254	12-13
CATGGGG	24675	0.0	18.419712	4
CAGAGTA	34730	0.0	17.74489	10-11
AGAGTAC	32485	0.0	17.2694	10-11
ATGGGGG	16870	0.0	16.39719	5
>>END_MODULE