FastQCFastQC Report
Fri 27 May 2016
SRR2140299_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140299_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8952092
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA113250.040.1493641
GTATCAA187600.031.8553471
ATCAACG215250.027.0529883
CAACGCA217850.026.7106155
TCAACGC218950.026.6518024
AACGCAG225600.025.9208096
TATCAAC230650.025.570392
ACGCAGA264150.022.0599787
CGCAGAG271300.021.4178818
GTACATG272950.021.2002531
TACATGG269800.020.9134852
ACATGGG279450.019.8210723
ACCGAAC12800.018.3385148
GCAGAGT318500.018.1274979
CATGGGG217750.017.8531784
GAGTACT160450.017.65900212-13
GGACCGA16450.017.2735846
CAGAGTA303000.016.71741310-11
AGAGTAC277100.016.64516310-11
TATACAC85250.015.7965543
Produced by FastQC (version 0.11.5)