##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140299_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8952092
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.976910424959886	31.0	31.0	34.0	30.0	34.0
2	32.084684227999446	33.0	31.0	34.0	30.0	34.0
3	31.815686098847063	31.0	31.0	34.0	30.0	34.0
4	35.886838852862546	37.0	35.0	37.0	35.0	37.0
5	35.82312726455447	37.0	35.0	37.0	35.0	37.0
6	35.866704899815595	37.0	35.0	37.0	35.0	37.0
7	35.85060776855287	37.0	35.0	37.0	35.0	37.0
8	35.84827557625636	37.0	35.0	37.0	35.0	37.0
9	37.61849498418917	39.0	37.0	39.0	35.0	39.0
10-11	37.52637176874411	39.0	37.0	39.0	35.0	39.0
12-13	37.475919650959796	39.0	37.0	39.0	34.0	39.0
14-15	38.71646577135266	40.0	38.0	41.0	34.0	41.0
16-17	38.656849650338714	40.0	38.0	41.0	34.0	41.0
18-19	38.656924381474184	40.0	38.0	41.0	34.0	41.0
20-21	38.718909380518866	40.0	38.0	41.0	34.0	41.0
22-23	38.674693823786235	40.0	38.0	41.0	34.0	41.0
24-25	38.57971192570921	40.0	38.0	41.0	34.0	41.0
26-27	38.425018875245186	40.0	38.0	41.0	34.0	41.0
28-29	38.29496040926523	40.0	38.0	41.0	34.0	41.0
30-31	38.1693199903556	40.0	38.0	41.0	33.5	41.0
32-33	37.97753518379453	40.0	37.0	41.0	33.0	41.0
34-35	38.06825753965252	40.0	37.0	41.0	33.0	41.0
36-37	38.025348689350466	40.0	37.0	41.0	33.0	41.0
38-39	37.98691240426342	40.0	37.0	41.0	33.0	41.0
40-41	37.923922192359925	40.0	37.0	41.0	33.0	41.0
42-43	37.95676696963913	40.0	37.0	41.0	33.0	41.0
44-45	37.98524338133737	40.0	37.0	41.0	33.0	41.0
46-47	37.929394188770175	40.0	37.0	41.0	33.0	41.0
48-49	37.83419613212952	40.0	37.0	41.0	33.0	41.0
50-51	37.730654199847656	40.0	37.0	41.0	33.0	41.0
52-53	37.45990111539105	39.5	36.0	41.0	32.0	41.0
54-55	37.392201188390025	39.0	36.0	41.0	32.0	41.0
56-57	37.22116199452742	39.0	35.5	41.0	31.5	41.0
58-59	37.04202713900521	39.0	35.0	41.0	31.0	41.0
60-61	36.88575499323419	39.0	35.0	40.0	31.0	41.0
62-63	36.62966063245527	38.0	35.0	40.0	31.0	41.0
64-65	36.26271470704708	38.0	35.0	40.0	30.0	41.0
66-67	36.011443150767334	37.0	34.0	40.0	30.0	41.0
68-69	35.71849321315362	37.0	34.0	39.5	30.0	41.0
70-71	35.441857782053276	36.0	34.0	39.0	30.0	41.0
72-73	35.148179561436024	36.0	34.0	39.0	29.0	40.0
74-75	34.82594554384257	35.0	34.0	38.0	29.0	40.0
76-77	33.59643535607654	34.5	32.0	36.5	28.0	39.0
78-79	34.22571210003562	35.0	33.0	37.0	29.0	39.0
80-81	34.16586356079924	35.0	34.0	36.5	29.0	39.0
82-83	33.89931607025187	35.0	33.0	36.0	29.0	37.5
84-85	33.67391975748883	35.0	33.0	36.0	29.0	37.0
86-87	33.48132288523985	35.0	33.0	35.0	29.0	37.0
88-89	33.32081021215141	35.0	33.0	35.0	29.0	36.0
90-91	33.1923879125812	35.0	33.0	35.0	29.0	36.0
92-93	33.1710443016576	35.0	33.0	35.0	29.0	36.0
94-95	33.17118796739845	35.0	33.0	35.0	29.0	35.5
96-97	33.16442592009211	35.0	33.0	35.0	29.0	35.0
98-99	33.27717823781833	35.0	33.0	35.0	29.0	35.0
100	32.130976153060836	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	8.0
20	64.0
21	343.0
22	1349.0
23	4854.0
24	12657.0
25	26921.0
26	48982.0
27	78590.0
28	112744.0
29	151013.0
30	192112.0
31	238906.0
32	297488.0
33	377502.0
34	504206.0
35	694658.0
36	1070315.0
37	1798157.0
38	2171584.0
39	1129472.0
40	40164.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.6889258957571	20.256561259647466	13.922310003069674	24.132202841525757
2	15.087846260971915	20.96985748673081	40.52813326932828	23.41416298296899
3	18.369954995899732	25.749914738303843	30.418256514769965	25.461873751026467
4	12.899356092844256	17.6211259366133	38.10953380934258	31.369984161199866
5	13.337820924986026	37.82208672565027	35.48451021280836	13.355582136555345
6	30.839115594433125	37.35467642647104	18.283804500668673	13.522403478427163
7	27.21029900050178	32.357743865903075	22.609016976143675	17.822940157451463
8	25.119346405287168	36.46396842212971	20.70936044893194	17.707324723651187
9	25.686677482760455	16.57296417418409	20.96728898675304	36.773069356302415
10-11	24.796159378165463	26.474286680699887	28.408147503399206	20.321406437735448
12-13	25.282554066691898	23.875084170269922	28.73528891347408	22.1070728495641
14-15	22.3364941991713	25.395151050319797	26.16661954541753	26.101735205091376
16-17	22.044636375203012	28.590976322685556	26.92278691712789	22.44160038498354
18-19	22.121844759321228	27.90039066427225	28.42297617989055	21.55478839651597
20-21	22.84735007362621	26.9512705248108	28.335894597138143	21.865484804424845
22-23	22.648661056271965	26.92445910239681	28.158265887616285	22.26861395371494
24-25	22.46540475094751	27.06545588975831	28.230596028651146	22.238543330643033
26-27	22.441519924323668	27.256745800298404	28.149836991878075	22.15189728349985
28-29	22.244372884210335	27.328668769832575	28.189607204811935	22.237351141145147
30-31	22.558578231074783	27.164684747263447	28.115062107266418	22.161674914395356
32-33	22.335477946655626	27.325193543282055	28.03198687339628	22.307341636666038
34-35	22.360375641506486	27.37725554078451	28.175518873990395	22.086849943718608
36-37	22.519011493260184	27.29120312183947	27.976105518901267	22.213679865999087
38-39	22.376883123283932	27.328722963458794	28.035614505529523	22.258779407727747
40-41	22.521828470376306	27.182067382244696	28.01096111576526	22.28514303161374
42-43	22.469357390776814	27.24901307535641	27.854975866085613	22.426653667781164
44-45	22.459463327974778	27.42558852112294	27.69923464976834	22.415713501133947
46-47	22.582945654485048	27.370349916684226	27.398751662548516	22.647952766282213
48-49	22.504939497658594	27.53289186257849	27.370517147191098	22.591651492571813
50-51	22.425005753893405	27.57706821725478	27.38703372815463	22.610892300697184
52-53	22.549167615003455	27.52700225177439	27.307592728049652	22.616237405172505
54-55	22.546195090850453	27.444888234242892	27.308611459154662	22.70030521575199
56-57	22.619909913557617	27.570615287912716	27.226201309954323	22.583273488575344
58-59	22.503070754530082	27.76962886368662	27.289862611694303	22.437437770088998
60-61	22.69755049168717	27.68275895595457	27.205009353556687	22.414681198801574
62-63	22.63159298934497	27.743918029328636	27.114681228581812	22.50980775274458
64-65	22.48870245994749	27.76850086693515	27.141604963046618	22.601191710070747
66-67	22.604325697104507	27.653760216981944	27.110649125096685	22.631264960816864
68-69	22.540485966521533	27.591464251806702	27.182884026392617	22.685165755279144
70-71	22.52136789399819	27.407725624219058	27.265651760502724	22.805254721280026
72-73	22.647536411803383	27.459366427463195	27.198825938160496	22.69427122257292
74-75	22.410432054834857	27.59830041641654	27.26586184499259	22.725405683756016
76-77	22.556304776411647	27.503278409579767	27.152497302299643	22.78791951170894
78-79	22.525989852676005	27.490901695943187	27.136258444152894	22.84685000722791
80-81	22.48783319631476	27.53732967953721	27.235302138183513	22.739534985964514
82-83	22.548891219142593	27.51909288397027	27.327418206799447	22.60459769008769
84-85	22.68032690459771	27.553450756341256	27.17696818883233	22.589254150228705
86-87	22.456388542266534	27.577479891782307	27.26193245075306	22.704199115198097
88-89	22.513281662367188	27.540679012560194	27.241734942848016	22.704304382224603
90-91	22.56988014849448	27.618697230477757	27.193048006695346	22.618374614332414
92-93	22.59772212726514	27.56391706931327	27.246055410798604	22.592305392622983
94-95	22.390670591960017	27.64188621453191	27.33921551301669	22.62822768049138
96-97	22.378654848049766	27.81680394746187	27.42068764970913	22.383853554779236
98-99	23.550106484260315	29.72861326101653	27.550037666023997	19.17124258869916
100	22.560418905978803	30.752479258218607	21.171827071470435	25.51527476433215
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1547.0
1	1437.0
2	2032.0
3	2894.0
4	3776.0
5	6068.5
6	8153.0
7	9589.0
8	10503.5
9	10248.5
10	9267.0
11	8262.0
12	8683.0
13	11238.5
14	17519.0
15	26563.5
16	34538.0
17	39780.0
18	41924.5
19	41258.0
20	38764.5
21	36040.5
22	35108.5
23	37358.0
24	43225.5
25	52876.0
26	66583.5
27	82463.5
28	98922.0
29	118526.0
30	139422.5
31	159973.0
32	181363.0
33	201524.5
34	223006.0
35	245564.0
36	268859.0
37	289948.5
38	305559.0
39	318762.0
40	332392.5
41	344981.5
42	354534.5
43	364553.5
44	375632.5
45	389413.5
46	400056.0
47	404295.0
48	405410.5
49	401161.5
50	394343.0
51	386095.0
52	372952.0
53	354925.0
54	336713.5
55	315175.0
56	292069.5
57	267660.0
58	240099.5
59	210360.0
60	175925.5
61	143865.5
62	116380.5
63	91836.5
64	70936.0
65	54509.0
66	41896.0
67	32344.5
68	24784.5
69	18088.0
70	12959.5
71	9538.0
72	7106.0
73	5109.5
74	3616.5
75	2582.5
76	1750.5
77	1081.0
78	665.0
79	458.0
80	331.5
81	218.5
82	143.5
83	89.0
84	54.0
85	36.5
86	29.0
87	22.0
88	14.5
89	9.5
90	6.5
91	3.5
92	1.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.007986959919536126
3	0.0026921081686828064
4	2.7926433285091354E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	1.0053515982632886E-4
16-17	2.3458203959476735E-4
18-19	0.0017872917302458463
20-21	7.819793965185719E-5
22-23	0.0017373088471475454
24-25	2.682098268057394E-4
26-27	0.002672147065135597
28-29	0.005617078918672027
30-31	0.002813844090504212
32-33	0.0021707735152134105
34-35	0.0023493561493465372
36-37	0.0028578923538198
38-39	0.0
40-41	5.785906307546263E-4
42-43	0.005983305409293732
44-45	0.0039282327819945406
46-47	6.218448846202693E-4
48-49	0.016147728055803258
50-51	0.010949857969942823
52-53	0.015020288515635749
54-55	0.012790903269481037
56-57	0.011229698041756666
58-59	0.017635354540912106
60-61	0.008907355743499183
62-63	0.020319566369470125
64-65	0.014846879076898436
66-67	0.0314159201397001
68-69	0.02171860751188609
70-71	0.021993164134695636
72-73	0.020135347331267456
74-75	0.010060313371379737
76-77	0.012246056214877088
78-79	0.012948030123300263
80-81	0.010150296684409239
82-83	0.004885644459068477
84-85	0.0034285214422506826
86-87	0.014050591562615964
88-89	0.0014131898335586713
90-91	2.354856829414485E-4
92-93	0.0010945199463477506
94-95	0.002763257993661135
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	276.0
20-21	822.0
22-23	1780.0
24-25	4004.0
26-27	5650.0
28-29	12742.0
30-31	27949.0
32-33	34795.0
34-35	47377.0
36-37	70814.0
38-39	83931.0
40-41	83420.0
42-43	89434.0
44-45	101344.0
46-47	99593.0
48-49	93020.0
50-51	85790.0
52-53	82064.0
54-55	87834.0
56-57	91672.0
58-59	90056.0
60-61	88364.0
62-63	86841.0
64-65	89750.0
66-67	93277.0
68-69	92610.0
70-71	89626.0
72-73	84027.0
74-75	85381.0
76-77	85954.0
78-79	91446.0
80-81	93004.0
82-83	90630.0
84-85	90595.0
86-87	96167.0
88-89	102826.0
90-91	109907.0
92-93	119230.0
94-95	148388.0
96-97	333192.0
98-99	571472.0
100-101	5115038.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.67052655593727
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.58607165357306	73.23457189122873
2	7.5002469497042	12.400987292632172
3	2.0737453124718144	5.143128507748546
4	0.8007956326019581	2.6480878644359507
5	0.396408041112254	1.638563074487883
6	0.21945847548529787	1.0885648635319698
7	0.13256806372805022	0.767163014302927
8	0.07412997187395133	0.49026910467171014
9	0.053286157506043846	0.39646752292505283
>10	0.1606448751100138	1.9792872816307432
>50	0.0020899689189451164	0.1139943420420038
>100	5.075196516822945E-4	0.07069482782848026
>500	4.73782627869238E-5	0.028220412533784794
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	6.702343988421924E-5	0.0	0.0	0.0
3	0.0	7.819401319825578E-5	0.0	0.0	0.0
4	0.0	1.340468797684385E-4	0.0	2.234114662807308E-5	0.0
5	1.117057331403654E-5	1.4521745308247502E-4	0.0	2.234114662807308E-5	0.0
6	1.117057331403654E-5	1.4521745308247502E-4	0.0	2.234114662807308E-5	0.0
7	1.117057331403654E-5	1.5638802639651156E-4	0.0	2.234114662807308E-5	0.0
8	1.117057331403654E-5	1.675585997105481E-4	0.0	2.234114662807308E-5	0.0
9	1.117057331403654E-5	3.127760527930231E-4	0.0	5.5852866570182704E-5	0.0
10-11	2.234114662807308E-5	3.351171994210962E-4	0.0	5.5852866570182704E-5	0.0
12-13	2.234114662807308E-5	3.4628777273513274E-4	0.0	6.143815322720098E-5	0.0
14-15	2.234114662807308E-5	4.524082192184799E-4	0.0	8.936458651229233E-5	0.0
16-17	2.7926433285091352E-5	4.74749365846553E-4	0.0	1.0053515982632886E-4	0.0
18-19	3.351171994210962E-5	5.417728057307723E-4	0.0	1.1729101979738367E-4	0.0
20-21	5.5852866570182704E-5	5.97625672300955E-4	0.0	1.619733130535298E-4	0.0
22-23	5.5852866570182704E-5	6.81404972156229E-4	0.0	2.457526129088039E-4	0.0
24-25	6.702343988421924E-5	7.4284312538343E-4	0.0	3.0719076613600483E-4	0.0
26-27	6.702343988421924E-5	7.595989853544847E-4	0.0	3.742142060202241E-4	0.0
28-29	7.260872654123751E-5	7.651842720115031E-4	0.0	0.0010165221715773252	0.0
30-31	9.49498731693106E-5	8.154518519246674E-4	0.0	0.0026585964487406966	0.0
32-33	1.0053515982632886E-4	8.545488585237954E-4	0.0	0.005831039269927073	0.0
34-35	1.1170573314036541E-4	9.830104516352156E-4	0.0	0.009723984069868808	0.0
36-37	1.1170573314036541E-4	0.001094716184775581	0.0	0.015622046779680102	0.0
38-39	1.5638802639651156E-4	0.0011282279047176904	0.0	0.02505001065672694	0.0
40-41	1.619733130535298E-4	0.0012734453578001656	0.0	0.03496947975959139	0.0
42-43	1.843144596816029E-4	0.00132371293771333	0.0	0.04376072095773815	0.0
44-45	2.1224089296669428E-4	0.0014801009641098417	0.0	0.0536522636273175	0.0
46-47	2.2341146628073082E-4	0.0015750508372791522	0.0	0.06439276986876363	0.0
48-49	2.2899675293774907E-4	0.0016085625572212618	0.0	0.07539019929643262	0.0
50-51	2.457526129088039E-4	0.0018263887368449743	0.0	0.08595197636485416	0.0
52-53	2.5133789956582214E-4	0.0018319740235019926	0.0	0.09634060954690814	0.0
54-55	2.569231862228404E-4	0.0018543151701300657	0.0	0.10580767043055411	0.0
56-57	2.569231862228404E-4	0.001882241603415157	0.0	0.1148558348149237	0.0
58-59	2.569231862228404E-4	0.0018934121767291935	0.0	0.12348510270001695	0.0
60-61	2.569231862228404E-4	0.001982776763241486	0.0	0.13216463816502333	0.0
62-63	2.625084728798587E-4	0.0019939473365555224	0.0	0.1399226013316217	0.0
64-65	2.68093759536877E-4	0.002189432369551162	0.0	0.14712203583251826	0.0
66-67	2.792643328509135E-4	0.0021950176562081803	0.0	0.15423769103355953	0.0
68-69	2.792643328509135E-4	0.0022285293761502897	0.0	0.16134217566128678	0.0
70-71	2.792643328509135E-4	0.0022620410960923995	0.0	0.16711736206464367	0.0
72-73	2.792643328509135E-4	0.0022843822427204724	0.0	0.171809002856539	0.0
74-75	2.792643328509135E-4	0.002368161542575746	0.0	0.17570194765648073	0.0
76-77	2.792643328509135E-4	0.002440770269116984	0.0	0.1792932869769435	0.0
78-79	2.792643328509135E-4	0.0024686967024020756	0.0	0.18167261909283328	0.0
80-81	2.792643328509135E-4	0.002474281989059094	0.0	0.18243221807818777	0.0
82-83	2.792643328509135E-4	0.0024910378490301485	0.0	0.18303542903714573	0.0
84-85	2.792643328509135E-4	0.00251896428231524	0.0	0.18319181706354223	0.0
86-87	2.792643328509135E-4	0.0025357201422862947	0.0	0.1833314492299677	0.0
88	2.792643328509135E-4	0.0025915730088564774	0.0	0.18335379037659577	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11325	0.0	40.149364	1
GTATCAA	18760	0.0	31.855347	1
ATCAACG	21525	0.0	27.052988	3
CAACGCA	21785	0.0	26.710615	5
TCAACGC	21895	0.0	26.651802	4
AACGCAG	22560	0.0	25.920809	6
TATCAAC	23065	0.0	25.57039	2
ACGCAGA	26415	0.0	22.059978	7
CGCAGAG	27130	0.0	21.417881	8
GTACATG	27295	0.0	21.200253	1
TACATGG	26980	0.0	20.913485	2
ACATGGG	27945	0.0	19.821072	3
ACCGAAC	1280	0.0	18.338514	8
GCAGAGT	31850	0.0	18.127497	9
CATGGGG	21775	0.0	17.853178	4
GAGTACT	16045	0.0	17.659002	12-13
GGACCGA	1645	0.0	17.273584	6
CAGAGTA	30300	0.0	16.717413	10-11
AGAGTAC	27710	0.0	16.645163	10-11
TATACAC	8525	0.0	15.796554	3
>>END_MODULE