FastQCFastQC Report
Fri 27 May 2016
SRR2140298_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140298_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8883178
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96700.042.9923741
GTATCAA164450.033.7492181
TCAACGC190050.028.347314
ATCAACG191200.028.199753
CAACGCA193800.027.8415575
AACGCAG201050.026.9612466
TATCAAC203600.026.7913842
ACGCAGA234000.023.0762317
CGCAGAG239450.022.6029268
GTACATG256550.021.6980651
CTTATAC66400.021.4112991
TACATGG254700.021.1233482
ACATGGG261550.020.139413
GCAGAGT289950.018.551889
CATGGGG198250.017.9562784
GAGTACT151450.017.55409412-13
GTGGTAT58450.017.3030361
TATACAC101950.016.7887633
CAGAGTA281050.016.75081410-11
AGAGTAC255700.016.7097210-11
Produced by FastQC (version 0.11.5)