##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140298_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8883178
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.95973164108611	31.0	31.0	34.0	30.0	34.0
2	32.06733750016041	33.0	31.0	34.0	30.0	34.0
3	31.798407844579945	31.0	31.0	34.0	30.0	34.0
4	35.87434857209886	37.0	35.0	37.0	35.0	37.0
5	35.80793878046798	37.0	35.0	37.0	35.0	37.0
6	35.85151744116801	37.0	35.0	37.0	35.0	37.0
7	35.835599939571175	37.0	35.0	37.0	35.0	37.0
8	35.83114342637286	37.0	35.0	37.0	35.0	37.0
9	37.601076439085205	39.0	37.0	39.0	35.0	39.0
10-11	37.505340431093465	39.0	37.0	39.0	34.5	39.0
12-13	37.45681230298436	39.0	37.0	39.0	34.0	39.0
14-15	38.69368569446655	40.0	38.0	41.0	34.0	41.0
16-17	38.62807364661611	40.0	38.0	41.0	34.0	41.0
18-19	38.62782069660205	40.0	38.0	41.0	34.0	41.0
20-21	38.69098469270459	40.0	38.0	41.0	34.0	41.0
22-23	38.647196847065246	40.0	38.0	41.0	34.0	41.0
24-25	38.55270639954462	40.0	38.0	41.0	34.0	41.0
26-27	38.39674934697497	40.0	38.0	41.0	34.0	41.0
28-29	38.26282578187988	40.0	38.0	41.0	34.0	41.0
30-31	38.13782606715043	40.0	38.0	41.0	33.0	41.0
32-33	37.94854229453513	40.0	37.0	41.0	33.0	41.0
34-35	38.04299873108472	40.0	37.0	41.0	33.0	41.0
36-37	38.00260752088171	40.0	37.0	41.0	33.0	41.0
38-39	37.960684170173195	40.0	37.0	41.0	33.0	41.0
40-41	37.901087811940506	40.0	37.0	41.0	33.0	41.0
42-43	37.93865563871561	40.0	37.0	41.0	33.0	41.0
44-45	37.973405287796595	40.0	37.0	41.0	33.0	41.0
46-47	37.91925963176646	40.0	37.0	41.0	33.0	41.0
48-49	37.827422730815016	40.0	37.0	41.0	33.0	41.0
50-51	37.72410150940968	40.0	37.0	41.0	32.5	41.0
52-53	37.45525853959674	39.5	36.0	41.0	32.0	41.0
54-55	37.395721888957056	39.0	36.0	41.0	32.0	41.0
56-57	37.23307668595943	39.0	35.5	41.0	31.0	41.0
58-59	37.06214222977599	39.0	35.0	41.0	31.0	41.0
60-61	36.91397737919512	39.0	35.0	40.0	31.0	41.0
62-63	36.670083857578874	38.0	35.0	40.0	31.0	41.0
64-65	36.314886572490224	38.0	35.0	40.0	30.0	41.0
66-67	36.07238722564083	37.0	34.5	40.0	30.0	41.0
68-69	35.78723057695069	37.0	34.0	39.5	30.0	41.0
70-71	35.51847652144295	36.5	34.0	39.0	30.0	41.0
72-73	35.22304890001605	36.0	34.0	39.0	29.0	40.5
74-75	34.898967403965614	35.5	34.0	38.5	29.0	40.0
76-77	33.6618103746041	34.5	32.0	36.5	28.0	39.0
78-79	34.29414764814478	35.0	33.5	37.0	29.0	39.0
80-81	34.22813097925105	35.0	34.0	37.0	29.0	39.0
82-83	33.95269677449709	35.0	33.0	36.0	29.0	38.5
84-85	33.715744896979466	35.0	33.0	36.0	29.0	37.0
86-87	33.509227311581206	35.0	33.0	35.5	29.0	37.0
88-89	33.33481341550463	35.0	33.0	35.0	29.0	36.5
90-91	33.199599727931286	35.0	33.0	35.0	29.0	36.0
92-93	33.171144873019436	35.0	33.0	35.0	29.0	36.0
94-95	33.16249846219961	35.0	33.0	35.0	29.0	36.0
96-97	33.15218198737408	35.0	33.0	35.0	29.0	35.0
98-99	33.26694118327461	35.0	33.0	35.0	29.0	35.0
100	32.11208616076232	34.0	31.0	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	4.0
19	12.0
20	49.0
21	294.0
22	1449.0
23	4796.0
24	12669.0
25	26942.0
26	49982.0
27	78573.0
28	113798.0
29	151770.0
30	193974.0
31	241920.0
32	298594.0
33	377940.0
34	502667.0
35	688403.0
36	1047359.0
37	1753525.0
38	2174495.0
39	1125392.0
40	38571.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.337007994211085	20.443235517739257	14.053585327233115	24.166171160816546
2	14.888679962618435	21.159188796158	40.813083953272816	23.13904728795075
3	18.287565281011034	25.86677636004194	30.55297101422355	25.292687344723475
4	12.67032971115278	17.86517130937611	38.37070431972302	31.09379465974809
5	13.16006501276908	37.915338407043066	35.732572284378406	13.192024295809452
6	30.525246707878644	37.70586382486088	18.485512729791072	13.283376737469405
7	26.999875495008656	32.39714435531968	22.76213535291086	17.8408447967608
8	24.80102278711515	36.825345613923304	20.925529129327362	17.44810246963418
9	25.46272291290347	16.71998467215224	21.146778776694557	36.67051363824974
10-11	24.44962827492593	26.72438287288626	28.64210308517965	20.183885767008157
12-13	25.01613724277505	24.058613932986596	28.961774716210797	21.963474108027555
14-15	22.07569043909384	25.675306544584114	26.3278945651099	25.92110845121215
16-17	21.84039535946485	28.947519949693557	27.08574367681128	22.126341014030317
18-19	21.83723327147941	28.17090866169415	28.586234498595104	21.40562356823134
20-21	22.55167922767117	27.23590298765086	28.538745200934134	21.67367258374383
22-23	22.316232185600764	27.318748550416245	28.37337562177292	21.991643642210075
24-25	22.134986316637452	27.443814541858902	28.456090012724744	21.965109128778902
26-27	22.13143039655344	27.6091314827844	28.344099129066187	21.915338991595977
28-29	21.929377918918913	27.633862402125807	28.387541330798143	22.049218348157137
30-31	22.260538521038303	27.425005233548994	28.415536618211938	21.898919627200765
32-33	22.0179954694415	27.64861317704551	28.293634068558326	22.03975728495466
34-35	22.064626748519952	27.70004619429306	28.47587036211913	21.759456695067854
36-37	22.244880700197285	27.583125098528072	28.215969069347807	21.956025131926836
38-39	22.0350640811822	27.621040615941105	28.362591250689288	21.981304052187404
40-41	22.206306724160953	27.46483910330874	28.287535699382243	22.041318473148063
42-43	22.166149833799693	27.55583438186633	28.178346072226706	22.09966971210727
44-45	22.14084102902179	27.702450901636087	28.028115323574625	22.128592745767495
46-47	22.264870590489764	27.613993680052257	27.726675151304242	22.39446057815374
48-49	22.198996442581045	27.67475847897601	27.81494387611926	22.311301202323687
50-51	22.143293226905715	27.824465263971078	27.739554045151348	22.29268746397186
52-53	22.247463325807406	27.80022286969795	27.72746538002006	22.224848424474583
54-55	22.197457096262394	27.75454684838607	27.67737328997652	22.370622765375014
56-57	22.298982898928788	27.901072325306618	27.532262346949167	22.26768242881543
58-59	22.16903023152068	28.04652412374274	27.71744379336829	22.06700185136829
60-61	22.35143462477554	28.073746560388596	27.492377950314573	22.082440864521296
62-63	22.282935924067175	28.182290727041643	27.427006934538806	22.10776641435238
64-65	22.223032401651206	28.094116518816627	27.459184624414796	22.223666455117367
66-67	22.263492593827813	28.011166731744215	27.42738739830004	22.29795327612793
68-69	22.218369600868655	27.935438637520004	27.556289267893895	22.28990249371744
70-71	22.2192125718067	27.752026052142774	27.574656813672377	22.454104562378145
72-73	22.31489821741124	27.79176639663163	27.53429698108415	22.359038404872976
74-75	22.142575514839834	27.910408383043244	27.538904598719306	22.40811150339761
76-77	22.224224561455227	27.84175460286255	27.51374734228443	22.420273493397797
78-79	22.215156598080547	27.79919736836538	27.489599201255377	22.496046832298696
80-81	22.208485603521623	27.876074377337982	27.529074847567596	22.3863651715728
82-83	22.204519071392482	27.85563558763019	27.664513666265883	22.275331674711445
84-85	22.32684183605671	27.854498303460012	27.54250517026029	22.276154690222985
86-87	22.180925980843224	27.870105981208816	27.611841651054682	22.337126386893278
88-89	22.222744977722314	27.804177356229104	27.602836457721207	22.370241208327375
90-91	22.256822380612327	27.89537161192836	27.57683220098574	22.270973806473567
92-93	22.251742675852196	27.87623369018911	27.61983754424736	22.252186089711333
94-95	22.084992118097563	27.927144445128114	27.7090093027605	22.27885413401382
96-97	22.100439369828333	28.062666766859984	27.778145430043132	22.058748433268555
98-99	23.209422688946628	30.06549563499008	27.936336647600267	18.788745028463023
100	22.24404527395262	31.109104250373658	21.60611769586759	25.04073277980614
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1544.0
1	1365.5
2	1803.5
3	2587.0
4	3416.0
5	5535.0
6	7502.0
7	8811.5
8	9686.5
9	9536.0
10	8664.0
11	7704.0
12	8094.0
13	10662.0
14	16825.5
15	25376.0
16	32715.5
17	37670.0
18	39949.5
19	39504.0
20	37542.0
21	35538.5
22	35157.0
23	38059.5
24	44850.0
25	55964.5
26	70623.5
27	86926.0
28	105150.5
29	126380.5
30	149208.5
31	170964.0
32	192850.5
33	214817.0
34	236113.0
35	256989.0
36	279178.0
37	299530.5
38	315573.5
39	329135.0
40	340851.5
41	352322.0
42	361641.0
43	369846.5
44	379226.5
45	390267.5
46	397639.5
47	399898.5
48	397488.5
49	390525.0
50	381725.0
51	369545.5
52	352994.0
53	333183.5
54	313390.0
55	291668.5
56	268470.0
57	243139.5
58	215004.0
59	185836.5
60	155488.5
61	127407.0
62	102852.0
63	81292.0
64	62251.5
65	47335.0
66	35928.0
67	27317.0
68	20980.5
69	15508.0
70	11377.5
71	8464.0
72	6087.5
73	4263.0
74	3010.0
75	2049.0
76	1401.5
77	905.5
78	568.0
79	369.0
80	252.0
81	179.0
82	131.5
83	93.5
84	62.0
85	41.5
86	29.5
87	22.5
88	14.5
89	10.5
90	7.0
91	3.0
92	2.5
93	1.5
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.008161493555572117
3	0.00264544963525441
4	4.390320671273276E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	9.568647616877655E-5
16-17	2.4203049854455241E-4
18-19	0.0017167279547927554
20-21	6.754585476999539E-5
22-23	0.0017113239657861306
24-25	2.9279376547478394E-4
26-27	0.002360213802699307
28-29	0.005552557550286019
30-31	0.0027218796374659615
32-33	0.0019994188657914158
34-35	0.0022402934170285727
36-37	0.002693099524264827
38-39	0.0
40-41	6.861863819450639E-4
42-43	0.005874178385241764
44-45	0.0036043987418277073
46-47	6.115808958436962E-4
48-49	0.015344729255256814
50-51	0.010568362879718981
52-53	0.014420720403760374
54-55	0.011931987421811124
56-57	0.010590260181284793
58-59	0.01634307610157538
60-61	0.008506258946669374
62-63	0.019069186010897386
64-65	0.014046273854396602
66-67	0.029705573326921295
68-69	0.020844471019284484
70-71	0.020295610635968162
72-73	0.01905611614048188
74-75	0.009652884243985126
76-77	0.01140093143271516
78-79	0.012175901767864927
80-81	0.009318016344657665
82-83	0.004605844551863465
84-85	0.0029363548076512466
86-87	0.013526625360738332
88-89	0.0011570301381064888
90-91	1.6346504802019308E-4
92-93	0.0010451788867213744
94-95	0.0026081222093395163
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	208.0
20-21	671.0
22-23	1492.0
24-25	3487.0
26-27	5182.0
28-29	11972.0
30-31	25337.0
32-33	31820.0
34-35	43177.0
36-37	65502.0
38-39	77430.0
40-41	76982.0
42-43	82672.0
44-45	94489.0
46-47	93248.0
48-49	87933.0
50-51	80555.0
52-53	76809.0
54-55	84134.0
56-57	88974.0
58-59	87792.0
60-61	86303.0
62-63	84201.0
64-65	87736.0
66-67	91813.0
68-69	89531.0
70-71	85432.0
72-73	80500.0
74-75	81411.0
76-77	81767.0
78-79	87617.0
80-81	89393.0
82-83	87177.0
84-85	87432.0
86-87	92967.0
88-89	100116.0
90-91	106956.0
92-93	117132.0
94-95	147322.0
96-97	332782.0
98-99	577896.0
100-101	5167828.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.43812369750802
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.41024295042448	75.49633154072185
2	7.192382103233575	12.146224994251588
3	1.8791796760497594	4.760232178083979
4	0.6912465498219603	2.3347024671736927
5	0.3273338015850419	1.3819726014306655
6	0.16902200745228654	0.8563140703714377
7	0.09900336661943625	0.5851760961957186
8	0.060314067562147494	0.4074245358009973
9	0.04341956636065749	0.3299640043728019
>10	0.12588866097127047	1.5251573713789
>50	0.001390291567914734	0.0775748506541115
>100	5.337518505493667E-4	0.07608725938565576
>500	4.320650092731835E-5	0.022838030178634068
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.1257232490444298E-5	0.0
2	0.0	3.377169747133289E-5	0.0	1.1257232490444298E-5	0.0
3	0.0	3.377169747133289E-5	0.0	1.1257232490444298E-5	0.0
4	0.0	5.628616245222149E-5	0.0	1.1257232490444298E-5	0.0
5	0.0	5.628616245222149E-5	0.0	1.1257232490444298E-5	0.0
6	0.0	5.628616245222149E-5	0.0	1.1257232490444298E-5	0.0
7	0.0	5.628616245222149E-5	0.0	2.2514464980888596E-5	0.0
8	0.0	5.628616245222149E-5	0.0	4.502892996177719E-5	0.0
9	0.0	1.6885848735666447E-4	0.0	6.754339494266578E-5	0.0
10-11	0.0	1.6885848735666447E-4	0.0	7.317201118788793E-5	0.0
12-13	0.0	1.6885848735666447E-4	0.0	9.005785992355438E-5	0.0
14-15	0.0	2.2514464980888596E-4	0.0	1.0131509241399868E-4	0.0
16-17	1.1257232490444298E-5	2.2514464980888596E-4	0.0	1.1257232490444298E-4	0.0
18-19	1.1257232490444298E-5	2.5891634728021884E-4	0.0	1.2945817364010942E-4	0.0
20-21	1.1257232490444298E-5	3.095738934872182E-4	0.0	1.4071540613055372E-4	0.0
22-23	1.1257232490444298E-5	3.320883584681068E-4	0.0	1.8011571984710877E-4	0.0
24-25	1.1257232490444298E-5	3.7148867218466183E-4	0.0	2.3640188229933026E-4	0.0
26-27	1.1257232490444298E-5	3.7148867218466183E-4	0.0	2.8143081226110744E-4	0.0
28-29	1.1257232490444298E-5	3.77117288429884E-4	0.0	7.035770306527687E-4	0.0
30-31	1.6885848735666446E-5	3.8274590467510613E-4	0.0	0.002369647439238525	0.0
32-33	2.8143081226110742E-5	3.9400313716555043E-4	0.0	0.0055667014665247054	0.0
34-35	3.377169747133289E-5	4.559179158629941E-4	0.0	0.009422303594501878	0.0
36-37	3.377169747133289E-5	5.178326945604377E-4	0.0	0.015231035559571136	0.0
38-39	3.377169747133289E-5	5.29089927050882E-4	0.0	0.024276221865643127	0.0
40-41	3.377169747133289E-5	6.078905544839921E-4	0.0	0.03365349653018323	0.0
42-43	3.377169747133289E-5	6.360336357101029E-4	0.0	0.04237785171027756	0.0
44-45	4.502892996177719E-5	7.373487281241015E-4	0.0	0.05189021316470299	0.0
46-47	5.628616245222149E-5	8.105207393119895E-4	0.0	0.06180783498878442	0.0
48-49	5.628616245222149E-5	8.161493555572117E-4	0.0	0.0720181448576174	0.0
50-51	5.628616245222149E-5	9.230930642164325E-4	0.0	0.0822847408889026	0.0
52-53	5.628616245222149E-5	9.456075291973211E-4	0.0	0.09147064260110514	0.0
54-55	6.754339494266578E-5	9.456075291973211E-4	0.0	0.10035822765231092	0.0
56-57	6.754339494266578E-5	9.456075291973211E-4	0.0	0.10881240925263458	0.0
58-59	6.754339494266578E-5	9.568647616877654E-4	0.0	0.11685007325081181	0.0
60-61	6.754339494266578E-5	9.681219941782097E-4	0.0	0.12481456523780116	0.0
62-63	6.754339494266578E-5	0.0010018936916495426	0.0	0.13225559591398484	0.0
64-65	6.754339494266578E-5	0.0011032087840635411	0.0	0.13937579546419088	0.0
66-67	6.754339494266578E-5	0.0011088374003087632	0.0	0.146321507910795	0.0
68-69	6.754339494266578E-5	0.0011426090977800962	0.0	0.1533685354498131	0.0
70-71	6.754339494266578E-5	0.0011594949465157625	0.0	0.1593404972859938	0.0
72-73	6.754339494266578E-5	0.001170752179006207	0.0	0.16384339028217154	0.0
74-75	6.754339494266578E-5	0.0011988952602323176	0.0	0.16760893455022516	0.0
76-77	6.754339494266578E-5	0.0012439241901940948	0.0	0.17114370555222466	0.0
78-79	6.754339494266578E-5	0.0012608100389297613	0.0	0.17347958129399188	0.0
80-81	6.754339494266578E-5	0.0012608100389297613	0.0	0.17426195895207774	0.0
82-83	6.754339494266578E-5	0.0012664386551749836	0.0	0.1747572771816573	0.0
84-85	6.754339494266578E-5	0.0012720672714202057	0.0	0.17491487843652348	0.0
86-87	6.754339494266578E-5	0.00128332450391065	0.0	0.1750387079939184	0.0
88	6.754339494266578E-5	0.0013846395963246487	0.0	0.17504996522640884	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9670	0.0	42.992374	1
GTATCAA	16445	0.0	33.749218	1
TCAACGC	19005	0.0	28.34731	4
ATCAACG	19120	0.0	28.19975	3
CAACGCA	19380	0.0	27.841557	5
AACGCAG	20105	0.0	26.961246	6
TATCAAC	20360	0.0	26.791384	2
ACGCAGA	23400	0.0	23.076231	7
CGCAGAG	23945	0.0	22.602926	8
GTACATG	25655	0.0	21.698065	1
CTTATAC	6640	0.0	21.411299	1
TACATGG	25470	0.0	21.123348	2
ACATGGG	26155	0.0	20.13941	3
GCAGAGT	28995	0.0	18.55188	9
CATGGGG	19825	0.0	17.956278	4
GAGTACT	15145	0.0	17.554094	12-13
GTGGTAT	5845	0.0	17.303036	1
TATACAC	10195	0.0	16.788763	3
CAGAGTA	28105	0.0	16.750814	10-11
AGAGTAC	25570	0.0	16.70972	10-11
>>END_MODULE