FastQCFastQC Report
Fri 27 May 2016
SRR2140297_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140297_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10056554
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA123200.034.80831
GTATCAA193250.028.7909931
ATCAACG210750.025.350793
TCAACGC211700.025.1975354
CAACGCA214600.024.8189285
AACGCAG220500.024.154846
TATCAAC235900.023.1697562
GTACATG278000.020.5726811
ACGCAGA259200.020.564167
CGCAGAG264400.020.159728
TACATGG274550.020.0866972
ACATGGG276850.019.416223
CGTCGAA5252.7785245E-718.56292294
CATGGGG208950.017.2346274
GCAGAGT320600.016.6512959
GAGTACT158100.016.43099612-13
AGAGTAC273500.015.99941610-11
CAGAGTA298350.015.74917310-11
CTCGATA10653.6379788E-1215.55625294
ATGGGGG140400.014.9063465
Produced by FastQC (version 0.11.5)