##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140297_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10056554
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.97204221247159	31.0	31.0	34.0	30.0	34.0
2	32.079265621205835	33.0	31.0	34.0	30.0	34.0
3	31.816174208381916	31.0	31.0	34.0	30.0	34.0
4	35.88554687818511	37.0	35.0	37.0	35.0	37.0
5	35.81821466876228	37.0	35.0	37.0	35.0	37.0
6	35.85838926534875	37.0	35.0	37.0	35.0	37.0
7	35.84137697664627	37.0	35.0	37.0	35.0	37.0
8	35.834922081659386	37.0	35.0	37.0	35.0	37.0
9	37.60980441212765	39.0	37.0	39.0	35.0	39.0
10-11	37.51397252975522	39.0	37.0	39.0	34.5	39.0
12-13	37.471299065266294	39.0	37.0	39.0	34.0	39.0
14-15	38.72070209139234	40.0	38.0	41.0	34.0	41.0
16-17	38.65932187109024	40.0	38.0	41.0	34.0	41.0
18-19	38.65214605321067	40.0	38.0	41.0	34.0	41.0
20-21	38.712621675908146	40.0	38.0	41.0	34.0	41.0
22-23	38.67373405653536	40.0	38.0	41.0	34.0	41.0
24-25	38.58501099367433	40.0	38.0	41.0	34.0	41.0
26-27	38.43993324070344	40.0	38.0	41.0	34.0	41.0
28-29	38.31157196113678	40.0	38.0	41.0	34.0	41.0
30-31	38.18991331690807	40.0	38.0	41.0	33.5	41.0
32-33	38.008234924629136	40.0	37.0	41.0	33.0	41.0
34-35	38.09896436448392	40.0	38.0	41.0	33.0	41.0
36-37	38.05809313002564	40.0	37.5	41.0	33.0	41.0
38-39	38.02539972484622	40.0	37.0	41.0	33.0	41.0
40-41	37.9659166483639	40.0	37.0	41.0	33.0	41.0
42-43	38.000285927656805	40.0	37.0	41.0	33.0	41.0
44-45	38.032070873690394	40.0	37.0	41.0	33.0	41.0
46-47	37.98254141417759	40.0	37.0	41.0	33.0	41.0
48-49	37.889484315882534	40.0	37.0	41.0	33.0	41.0
50-51	37.79297413805867	40.0	37.0	41.0	33.0	41.0
52-53	37.52555374996199	40.0	36.0	41.0	32.0	41.0
54-55	37.4720908656475	39.0	36.0	41.0	32.0	41.0
56-57	37.305927619667266	39.0	36.0	41.0	31.5	41.0
58-59	37.13508683554707	39.0	35.0	41.0	31.0	41.0
60-61	36.98681192415782	39.0	35.0	40.5	31.0	41.0
62-63	36.73979891664644	38.5	35.0	40.0	31.0	41.0
64-65	36.38130655433699	38.0	35.0	40.0	30.5	41.0
66-67	36.14051916362534	37.5	35.0	40.0	30.0	41.0
68-69	35.853215893767576	37.0	34.0	39.5	30.0	41.0
70-71	35.58000428136158	36.5	34.0	39.0	30.0	41.0
72-73	35.28181611750723	36.0	34.0	39.0	30.0	40.5
74-75	34.95823448082943	35.5	34.0	38.5	29.0	40.0
76-77	33.715791911805816	34.5	32.5	36.5	28.5	39.0
78-79	34.335179835220806	35.0	33.5	37.0	29.0	39.0
80-81	34.266910109324	35.0	34.0	37.0	29.0	39.0
82-83	33.990621512657256	35.0	33.5	36.0	29.0	38.0
84-85	33.75027219215124	35.0	33.0	36.0	29.0	37.0
86-87	33.54859203223887	35.0	33.0	35.5	29.0	37.0
88-89	33.37934332097254	35.0	33.0	35.0	29.0	36.5
90-91	33.24885082442767	35.0	33.0	35.0	29.0	36.0
92-93	33.2184215513339	35.0	33.0	35.0	29.0	36.0
94-95	33.211558909891814	35.0	33.0	35.0	29.0	36.0
96-97	33.201293357151314	35.0	33.0	35.0	29.0	35.0
98-99	33.30802571546982	35.0	33.0	35.0	29.5	35.0
100	32.19856800527387	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	13.0
20	56.0
21	301.0
22	1522.0
23	5085.0
24	13581.0
25	29453.0
26	53830.0
27	85244.0
28	122415.0
29	165326.0
30	211213.0
31	264709.0
32	327567.0
33	415310.0
34	552850.0
35	758277.0
36	1160103.0
37	1955497.0
38	2490046.0
39	1392207.0
40	51946.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.51949067245102	20.47076960955015	13.68845630421713	24.3212834137817
2	15.10005961760206	21.19994968055606	40.46335655552467	23.236634146317208
3	18.31196448397299	25.70538582511171	30.552663229685255	25.42998646123004
4	12.729658518608897	17.624220815438203	38.52052208584949	31.125598580103407
5	13.20309123781367	37.84417604678501	35.828018225726225	13.12471448967509
6	30.76170028023516	37.674326613271305	18.40514156240796	13.158831544085578
7	27.12119877246222	32.33315308603723	22.88960015528182	17.65604798621874
8	24.853135577057508	36.67675826132888	21.011322566358217	17.458783595255394
9	25.5726862302932	16.524308426126883	21.201139078057953	36.701866265521964
10-11	24.517021436965386	26.614618685486104	28.804722770841785	20.06363710670673
12-13	25.027986723881757	23.97110381945943	29.02510144130882	21.97580801534999
14-15	22.04242719753291	25.640079452738462	26.371269972503974	25.946223377224655
16-17	21.901224424004536	28.767184925567474	27.147773567299517	22.18381708312847
18-19	21.88806632682284	28.154453926978128	28.558550469035392	21.398929277163642
20-21	22.598821037005333	27.319660109889103	28.45346578684314	21.628053066262424
22-23	22.369612380680483	27.27182175943204	28.231062327537977	22.1275035323495
24-25	22.19523300380567	27.352928185677243	28.36935535985153	22.082483450665556
26-27	22.17898240203565	27.569119078702887	28.223292895150067	22.0286056241114
28-29	22.076967742260003	27.656416130827242	28.222687478805845	22.043928648106903
30-31	22.38819026255848	27.456224367885472	28.19039329829706	21.965192071258986
32-33	22.084903833231486	27.635355410160596	28.138200786446667	22.141539970161254
34-35	22.21067494302327	27.662914619546125	28.22828147886935	21.898128958561255
36-37	22.28227811775448	27.551008736256755	28.08456267344337	22.082150472545397
38-39	22.165422930742302	27.6661278395751	28.124383705979078	22.044065523703512
40-41	22.33225175422336	27.52595704738138	28.074982308022374	22.066808890372887
42-43	22.249523359464966	27.587928626595033	27.947645825524415	22.214902188415586
44-45	22.280803613109555	27.72214601685818	27.795388571177625	22.201661798854637
46-47	22.321087989744264	27.665278829506555	27.571394576171954	22.442238604577224
48-49	22.298557460180483	27.704512210425484	27.648042160844906	22.348888168549127
50-51	22.217868668463122	27.795284931801916	27.601206457400867	22.385639942334095
52-53	22.334971345382513	27.786141323722287	27.562768507500383	22.316118823394817
54-55	22.351448075519365	27.68836784027408	27.543029968280266	22.417154115926287
56-57	22.399489571378176	27.78737992151968	27.433881407687217	22.379249099414935
58-59	22.291639852088878	27.93921362328652	27.549425087818218	22.21972143680638
60-61	22.497245274541747	27.884382439584403	27.413430448659177	22.20494183721468
62-63	22.424387806870943	27.979608971714537	27.35123015889777	22.24477306251675
64-65	22.311660469992532	27.940855336959075	27.406292002104628	22.341192190943772
66-67	22.37542492799426	27.852191761722985	27.3363837005206	22.435999609762153
68-69	22.298866181451068	27.831485563800936	27.434223392280227	22.43542486246777
70-71	22.297155519360988	27.647496677464524	27.503368849048513	22.551978954125975
72-73	22.39969111285189	27.664639526996854	27.500313365586425	22.435355994564826
74-75	22.25841289631458	27.806459277512445	27.447667521116703	22.487460305056267
76-77	22.347124009606308	27.761793638901434	27.351408873516974	22.539673477975285
78-79	22.274511165118245	27.74684490191257	27.382663995647015	22.59597993732217
80-81	22.251486918296322	27.803773587890706	27.457213813135773	22.4875256806772
82-83	22.314363386429036	27.74061553931899	27.571667165887526	22.373353908364447
84-85	22.454147437691613	27.766484032085536	27.408578292575502	22.370790237647352
86-87	22.24163252813745	27.818616792303057	27.498351331980636	22.44139934757885
88-89	22.34957753011217	27.741168892763646	27.476198284009186	22.433055293115
90-91	22.34955178787614	27.77704600320406	27.499674611084718	22.37372759783508
92-93	22.37604624768187	27.758432144458205	27.519762405943847	22.345759201916078
94-95	22.208730564660968	27.837850259497454	27.576801094412918	22.37661808142866
96-97	22.19152321657716	27.98941495176085	27.648876003992207	22.17018582766979
98-99	23.325430432175008	29.914106749499286	27.84696583087736	18.913496987448347
100	29.922695445780263	41.375995436145665	28.70130911807407	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1371.0
1	1242.0
2	1731.0
3	2512.0
4	3386.0
5	5578.0
6	7487.0
7	8643.5
8	9383.5
9	9175.0
10	8350.5
11	7463.5
12	7896.0
13	10576.0
14	16816.5
15	25627.5
16	33463.0
17	38683.5
18	40931.0
19	41031.5
20	40064.0
21	38819.0
22	39143.5
23	42722.5
24	50550.5
25	62468.0
26	78594.5
27	97761.0
28	118908.0
29	144284.5
30	171139.0
31	196014.5
32	221606.0
33	248056.0
34	274008.5
35	300070.0
36	325571.0
37	349410.5
38	368907.5
39	383756.0
40	398110.0
41	412595.0
42	424656.5
43	434452.0
44	444033.0
45	454503.0
46	461320.5
47	464812.5
48	462937.5
49	452684.5
50	442470.0
51	430186.5
52	410177.0
53	385886.0
54	360499.0
55	334981.0
56	309402.5
57	280330.0
58	247541.5
59	211703.0
60	175578.0
61	142838.0
62	114396.0
63	89482.5
64	67919.5
65	51619.5
66	38788.0
67	29624.0
68	22708.0
69	16649.5
70	12434.5
71	9260.5
72	6670.5
73	4861.5
74	3503.0
75	2403.0
76	1626.5
77	1049.0
78	685.5
79	469.0
80	343.5
81	233.0
82	167.0
83	114.5
84	68.5
85	44.0
86	32.5
87	27.0
88	17.5
89	10.5
90	9.0
91	7.0
92	4.5
93	2.5
94	1.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.007945067465455861
3	0.002475997245179611
4	4.6735690973269775E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	9.446575835022613E-5
16-17	2.933410390875443E-4
18-19	0.0016904398862672043
20-21	3.4805231663870565E-5
22-23	0.001770308182309718
24-25	2.2382735728382217E-4
26-27	0.002756665732186839
28-29	0.005811634324199556
30-31	0.00283926879397592
32-33	0.002082940695674153
34-35	0.002298227332500842
36-37	0.0027937425430608342
38-39	0.0
40-41	6.345700777405096E-4
42-43	0.006181597509306145
44-45	0.003990671633726141
46-47	6.565662577575572E-4
48-49	0.016550783265953148
50-51	0.011102242800638321
52-53	0.015318436135770858
54-55	0.013275797427870124
56-57	0.011445103819645069
58-59	0.017471272579558977
60-61	0.00877401306551383
62-63	0.020417130001397703
64-65	0.014912066889643225
66-67	0.03175988994073732
68-69	0.02208657107233136
70-71	0.022132986465910293
72-73	0.019504922663513193
74-75	0.009472821045366328
76-77	0.012519471883523758
78-79	0.01270607399731528
80-81	0.010018794467463503
82-83	0.005228173678592474
84-85	0.00309172832495041
86-87	0.013814975102772957
88-89	0.0011252317994979509
90-91	2.7722845472859334E-4
92-93	0.0010785662394579198
94-95	0.0027191112289392644
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	376.0
20-21	1049.0
22-23	1976.0
24-25	3613.0
26-27	6329.0
28-29	15299.0
30-31	32743.0
32-33	40267.0
34-35	56053.0
36-37	84858.0
38-39	98810.0
40-41	97378.0
42-43	104483.0
44-45	116922.0
46-47	115244.0
48-49	109037.0
50-51	100083.0
52-53	97784.0
54-55	104681.0
56-57	108598.0
58-59	106615.0
60-61	104504.0
62-63	101234.0
64-65	105441.0
66-67	109463.0
68-69	108312.0
70-71	104948.0
72-73	99404.0
74-75	100485.0
76-77	100684.0
78-79	107068.0
80-81	107646.0
82-83	104937.0
84-85	105406.0
86-87	111318.0
88-89	118356.0
90-91	125267.0
92-93	134519.0
94-95	167069.0
96-97	367211.0
98-99	2057102.0
100-101	4213982.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.92724919472457
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.24654717388441	74.90217204431337
2	7.259294427272596	12.185052247511651
3	1.9051297030368632	4.796768860051393
4	0.7275164587687881	2.4423382051340656
5	0.3320281207572369	1.3933103415224362
6	0.1799299868839512	0.9060617308087736
7	0.09794252401103554	0.5754032633604131
8	0.06540697377182286	0.43915419094564734
9	0.03950857113326214	0.29842611253459134
>10	0.1435027706835291	1.8198663825488002
>50	0.0026157021729404355	0.1480076962144833
>100	5.372000769608879E-4	0.07262668724923212
>500	4.03875466332131E-5	0.02081223780510531
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.943764036865909E-6
2	0.0	9.943764036865909E-6	0.0	0.0	9.943764036865909E-6
3	0.0	9.943764036865909E-6	0.0	2.9831292110597727E-5	9.943764036865909E-6
4	0.0	4.9718820184329545E-5	0.0	2.9831292110597727E-5	2.9831292110597727E-5
5	0.0	4.9718820184329545E-5	0.0	2.9831292110597727E-5	2.9831292110597727E-5
6	1.8893151670045227E-4	4.9718820184329545E-5	0.0	2.9831292110597727E-5	3.9775056147463636E-5
7	1.8893151670045227E-4	4.9718820184329545E-5	0.0	5.9662584221195454E-5	3.9775056147463636E-5
8	1.8893151670045227E-4	5.9662584221195454E-5	0.0	6.960634825806136E-5	5.9662584221195454E-5
9	1.8893151670045227E-4	1.1932516844239091E-4	0.0	9.943764036865909E-5	6.960634825806136E-5
10-11	2.1876280881104999E-4	1.1932516844239091E-4	0.0	9.943764036865909E-5	6.960634825806136E-5
12-13	2.485941009216477E-4	1.1932516844239091E-4	0.0	1.1932516844239091E-4	6.960634825806136E-5
14-15	2.5853786495851365E-4	1.4915646055298863E-4	0.0	1.2926893247925682E-4	1.392126965161227E-4
16-17	4.971882018432954E-4	1.4915646055298863E-4	0.0	1.5910022458985454E-4	2.3865033688478182E-4
18-19	4.971882018432954E-4	1.4915646055298863E-4	0.0	1.8395963468201932E-4	2.3865033688478182E-4
20-21	4.971882018432954E-4	1.4915646055298863E-4	0.0	2.5853786495851365E-4	2.3865033688478182E-4
22-23	5.021600838617283E-4	1.6904398862672043E-4	0.0	3.3808797725344087E-4	2.635097469769466E-4
24-25	5.071319658801613E-4	2.0384716275575113E-4	0.0	3.977505614746363E-4	3.2317233119814203E-4
26-27	5.170757299170273E-4	2.1876280881104999E-4	0.0	5.319913759723261E-4	3.28144213216575E-4
28-29	5.170757299170273E-4	2.1876280881104999E-4	0.0	8.999106453363647E-4	3.331160952350079E-4
30-31	5.220476119354603E-4	2.485941009216477E-4	0.0	0.002908550980783278	3.5300362330873975E-4
32-33	5.270194939538932E-4	2.485941009216477E-4	0.0	0.006224796287078059	3.878067974377704E-4
34-35	5.46907022027625E-4	3.082566851428432E-4	0.0	0.010311683306229946	3.9775056147463636E-4
36-37	5.46907022027625E-4	3.6791926936403864E-4	0.0	0.01716293672763056	3.9775056147463636E-4
38-39	5.46907022027625E-4	3.6791926936403864E-4	0.0	0.02678352843329832	4.027224434930693E-4
40-41	5.46907022027625E-4	3.878067974377704E-4	0.0	0.03683170199255133	4.1763808954836814E-4
42-43	5.46907022027625E-4	3.977505614746363E-4	0.0	0.04689479119785962	4.275818535852341E-4
44-45	5.618226680829239E-4	4.3752561762209997E-4	0.0	0.05741529354886375	4.424974996405329E-4
46-47	5.767383141382227E-4	4.673569097326977E-4	0.0	0.0685871124442826	4.7730067376956363E-4
48-49	5.817101961566556E-4	4.673569097326977E-4	0.0	0.07940095583437429	5.170757299170273E-4
50-51	6.364008983594182E-4	5.270194939538932E-4	0.0	0.09000598017969177	5.170757299170273E-4
52-53	6.662321904700158E-4	5.369632579907591E-4	0.0	0.10027291654775583	5.369632579907591E-4
54-55	6.662321904700158E-4	5.419351400091921E-4	0.0	0.1100923835341609	5.717664321197897E-4
56-57	6.761759545068817E-4	5.46907022027625E-4	0.0	0.11916606821780104	5.966258422119545E-4
58-59	6.761759545068817E-4	5.46907022027625E-4	0.0	0.1276381551772108	6.115414882672534E-4
60-61	7.308666567096443E-4	5.46907022027625E-4	0.0	0.13629420177130258	6.264571343225523E-4
62-63	8.352761790967363E-4	5.518789040460579E-4	0.0	0.14486572637108097	6.264571343225523E-4
64-65	8.352761790967363E-4	6.165133702856863E-4	0.0	0.1529251471229608	6.314290163409852E-4
66-67	8.501918251520352E-4	6.463446623962841E-4	0.0	0.16053212661116323	6.413727803778512E-4
68-69	8.601355891889012E-4	6.662321904700158E-4	0.0	0.168074471633126	6.463446623962841E-4
70-71	8.849949992810659E-4	6.861197185437477E-4	0.0	0.17429926792020406	6.761759545068817E-4
72-73	0.0010341514598340546	7.010353645990465E-4	0.0	0.17988766330892272	7.65669830838675E-4
74-75	0.0010440952238709204	7.109791286359125E-4	0.0	0.18406901608642484	7.65669830838675E-4
76-77	0.0010490671058893534	7.358385387280773E-4	0.0	0.18794708406080252	7.805854768939738E-4
78-79	0.0010540389879077864	7.557260668018091E-4	0.0	0.1904877157722218	7.955011229492727E-4
80-81	0.0010540389879077864	7.557260668018091E-4	0.0	0.19126830124911576	8.104167690045716E-4
82-83	0.0010540389879077864	7.557260668018091E-4	0.0	0.19184006768123554	8.352761790967363E-4
84-85	0.0010590108699262192	7.557260668018091E-4	0.0	0.1920737461361019	8.352761790967363E-4
86-87	0.0010739265159815181	7.557260668018091E-4	0.0	0.19213340872032308	8.352761790967363E-4
88	0.0011137015721289817	7.955011229492727E-4	0.0	0.19214335248435996	8.452199431336023E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12320	0.0	34.8083	1
GTATCAA	19325	0.0	28.790993	1
ATCAACG	21075	0.0	25.35079	3
TCAACGC	21170	0.0	25.197535	4
CAACGCA	21460	0.0	24.818928	5
AACGCAG	22050	0.0	24.15484	6
TATCAAC	23590	0.0	23.169756	2
GTACATG	27800	0.0	20.572681	1
ACGCAGA	25920	0.0	20.56416	7
CGCAGAG	26440	0.0	20.15972	8
TACATGG	27455	0.0	20.086697	2
ACATGGG	27685	0.0	19.41622	3
CGTCGAA	525	2.7785245E-7	18.562922	94
CATGGGG	20895	0.0	17.234627	4
GCAGAGT	32060	0.0	16.651295	9
GAGTACT	15810	0.0	16.430996	12-13
AGAGTAC	27350	0.0	15.999416	10-11
CAGAGTA	29835	0.0	15.749173	10-11
CTCGATA	1065	3.6379788E-12	15.556252	94
ATGGGGG	14040	0.0	14.906346	5
>>END_MODULE