FastQCFastQC Report
Fri 27 May 2016
SRR2140296_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140296_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1955548
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18500.041.5376241
GTATCAA31400.033.299061
ATCAACG37150.028.595773
CAACGCA37800.028.1040445
TCAACGC37900.028.029894
AACGCAG39350.026.8903166
TATCAAC41000.026.5263922
ACGCAGA45200.023.2236567
CGCAGAG46700.022.4777138
TACATGG51800.020.914732
ACATGGG52250.020.3317283
ACGCGAT1050.00449178119.9944618
GTACATG50700.019.9604991
TATACCG1551.0091234E-418.9629755
CATGGGG38250.018.8815274
GCAGAGT58450.017.9590959
TAGACCG1651.6293357E-417.8137025
CTATACG2153.5110297E-617.5769754
ATACGAC1200.00970677217.49563
TACGCGA1200.00970820117.4951527
Produced by FastQC (version 0.11.5)