##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140296_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1955548
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.246740044222896	25.0	18.0	31.0	18.0	33.0
2	30.91590745918791	32.0	30.0	33.0	27.0	33.0
3	31.12285967923058	33.0	31.0	33.0	28.0	33.0
4	32.24511287884521	33.0	33.0	33.0	31.0	34.0
5	32.62408900216205	33.0	33.0	34.0	31.0	34.0
6	36.52611339634721	38.0	37.0	38.0	34.0	38.0
7	36.81466780667107	38.0	38.0	38.0	35.0	38.0
8	36.82829416613655	38.0	38.0	38.0	35.0	38.0
9	37.17197276671296	38.0	38.0	38.0	36.0	38.0
10-11	37.220536903210764	38.0	38.0	38.0	36.0	38.0
12-13	37.27616708973648	38.0	38.0	38.0	36.5	38.0
14-15	37.27052877249753	38.0	38.0	38.0	36.5	38.0
16-17	37.27653527297719	38.0	38.0	38.0	37.0	38.0
18-19	37.30872036891961	38.0	38.0	38.0	37.0	38.0
20-21	37.28806979234122	38.0	38.0	38.0	37.0	38.0
22-23	37.319142170763385	38.0	38.0	38.0	37.0	38.0
24-25	37.34650713115095	38.0	38.0	38.0	37.0	38.0
26-27	37.32767988716609	38.0	38.0	38.0	37.0	38.0
28-29	37.306448827378475	38.0	38.0	38.0	37.0	38.0
30-31	37.307947956570224	38.0	38.0	38.0	37.0	38.0
32-33	37.25104823151751	38.0	38.0	38.0	37.0	38.0
34-35	37.26710890979699	38.0	38.0	38.0	37.0	38.0
36-37	37.268672630787535	38.0	38.0	38.0	37.0	38.0
38-39	37.2618594808587	38.0	38.0	38.0	37.0	38.0
40-41	37.254900567486914	38.0	38.0	38.0	37.0	38.0
42-43	37.239842564430546	38.0	38.0	38.0	37.0	38.0
44-45	37.22592484129018	38.0	38.0	38.0	37.0	38.0
46-47	37.2091571102705	38.0	38.0	38.0	37.0	38.0
48-49	37.17711361388044	38.0	38.0	38.0	36.5	38.0
50-51	37.1483728863141	38.0	38.0	38.0	36.0	38.0
52-53	37.11899367312401	38.0	38.0	38.0	36.0	38.0
54-55	37.09313500120412	38.0	38.0	38.0	36.0	38.0
56-57	37.06379724791819	38.0	38.0	38.0	36.0	38.0
58-59	37.06381806244898	38.0	38.0	38.0	36.0	38.0
60-61	37.085618282372934	38.0	38.0	38.0	36.0	38.0
62-63	37.089526198426874	38.0	38.0	38.0	36.0	38.0
64-65	37.08277491902149	38.0	38.0	38.0	36.0	38.0
66-67	37.08364335437419	38.0	38.0	38.0	36.0	38.0
68-69	37.083347063403906	38.0	38.0	38.0	36.0	38.0
70-71	37.06806208360638	38.0	38.0	38.0	36.0	38.0
72-73	37.04609506309744	38.0	38.0	38.0	36.0	38.0
74-75	37.03559362313734	38.0	38.0	38.0	36.0	38.0
76-77	37.030308635728254	38.0	38.0	38.0	36.0	38.0
78-79	37.006265622041184	38.0	38.0	38.0	36.0	38.0
80-81	36.97662676316309	38.0	38.0	38.0	36.0	38.0
82-83	36.97277279080866	38.0	38.0	38.0	36.0	38.0
84-85	36.91917204734726	38.0	38.0	38.0	35.5	38.0
86-87	36.90207178935242	38.0	38.0	38.0	35.0	38.0
88-89	36.906386799786866	38.0	38.0	38.0	35.0	38.0
90-91	36.884039199698705	38.0	38.0	38.0	35.0	38.0
92-93	36.88705059360566	38.0	38.0	38.0	35.0	38.0
94-95	36.83386054560559	38.0	38.0	38.0	35.0	38.0
96-97	36.80444546336813	38.0	38.0	38.0	35.0	38.0
98-99	36.87666341159989	38.0	38.0	38.0	35.0	38.0
100	35.2899159618683	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	5.0
19	13.0
20	18.0
21	54.0
22	183.0
23	439.0
24	1034.0
25	1939.0
26	3381.0
27	5222.0
28	7769.0
29	11386.0
30	15897.0
31	21497.0
32	29250.0
33	40396.0
34	59898.0
35	107773.0
36	325525.0
37	1323869.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.03514513578802	18.977340366996874	17.371140979408327	24.61637351780677
2	14.727827895448192	20.96957876440354	40.6208818266753	23.681711513472965
3	18.17720659375275	25.073483238457968	30.733840335292207	26.01546983249708
4	12.50790059870686	17.292799767635465	38.40319951236175	31.796100121295922
5	13.389394790927822	37.25553868552519	35.73426991832667	13.620796605220322
6	30.845141221645346	37.49787143519728	18.36359517535539	13.293392167801986
7	27.318991914286944	32.10619222846998	22.756383376935773	17.81843248030731
8	24.904937132711648	36.56581173154533	20.883813642007254	17.64543749373577
9	25.658025269643087	16.628331291279988	21.032621035126727	36.681022403950195
10-11	24.58932227692698	26.49461941102954	28.65565048774052	20.260407824302956
12-13	25.14719659144138	23.776890160711986	28.948637415190014	22.127275832656625
14-15	22.134588360909575	25.398277106979734	26.360718325502617	26.106416206608067
16-17	22.025258393043792	28.518527798857406	27.058042042435165	22.39817176566364
18-19	22.016156085148513	27.93061075463246	28.54391710149789	21.509316058721136
20-21	22.74113694690056	27.053198645967015	28.479949925632507	21.72571448149992
22-23	22.535254844904255	26.938792730917637	28.28738803470381	22.238564389474302
24-25	22.34228437973317	26.99296547022465	28.41889679936439	22.24585335067779
26-27	22.283996399087496	27.22568616819944	28.262636440825347	22.227680991887716
28-29	22.218910134298202	27.325322401907968	28.291971609748934	22.1637958540449
30-31	22.432825272160983	27.128713784689285	28.287777984494216	22.150682958655516
32-33	22.2443871690569	27.238669623515687	28.230156262446904	22.28678694498051
34-35	22.37258206587549	27.218661490253997	28.314274191718425	22.094482252152083
36-37	22.37648897003553	27.1999017116481	28.214925936409095	22.20868338190727
38-39	22.267413870074275	27.272976622334443	28.31194044315505	22.147669064436236
40-41	22.442853424684962	27.052224061571728	28.2824573475222	22.222465166221113
42-43	22.292185248046664	27.199101522488096	28.161050341244586	22.347662888220654
44-45	22.342296332280917	27.27958677905182	28.0433499363754	22.334766952291865
46-47	22.377260702179342	27.277955574199748	27.88135077166654	22.46343295195437
48-49	22.366759030634825	27.331698171280898	27.921881786694726	22.37966101138955
50-51	22.29196564590555	27.399184200649106	27.845003642947034	22.46384651049831
52-53	22.422978036846683	27.394253263090302	27.756978194456316	22.4257905056067
54-55	22.42536375117832	27.319276389082052	27.76478290127125	22.490576958468374
56-57	22.40880776293181	27.352210380059024	27.806714185982322	22.432267671026842
58-59	22.41218441980856	27.434653783868406	27.79643818969718	22.35672360662585
60-61	22.479638925456044	27.36430049040172	27.815904060642005	22.340156523500223
62-63	22.44819511345915	27.41032963572788	27.76037390242399	22.381101348388977
64-65	22.42060725100736	27.397703327783635	27.758664558430578	22.42302486277842
66-67	22.406586865751102	27.426326679551615	27.597060659990753	22.570025794706535
68-69	22.42451555296697	27.397114797972076	27.69356521487992	22.484804434181026
70-71	22.40067172405323	27.239328446188203	27.71232602693584	22.647673802822723
72-73	22.516161724736268	27.26628073031034	27.65293367934524	22.56462386560816
74-75	22.367333217972263	27.392548295044	27.720645104069952	22.519473382913784
76-77	22.46958896558636	27.306853914285355	27.569466193954124	22.65409092617416
78-79	22.411276191826403	27.299562690288585	27.570290281369985	22.71887083651503
80-81	22.42816202246616	27.353061590030965	27.585047721399146	22.633728666103732
82-83	22.449421526281157	27.37517469272826	27.73619555760109	22.4392082233895
84-85	22.621181168319456	27.29609774537705	27.577623652354678	22.505097433948812
86-87	22.441352512747734	27.430642570175184	27.55548198698452	22.572522930092564
88-89	22.49164528898285	27.32879549847137	27.59745940795286	22.582099804592918
90-91	22.532606340261204	27.381675743969836	27.588969197830615	22.49674871793835
92-93	22.600707498867802	27.38240753393265	27.58533382994333	22.43155113725621
94-95	22.550711428496577	27.362902302852472	27.57462794613027	22.511758322520688
96-97	22.507153847662984	27.555626245442138	27.64702873590601	22.29019117098887
98-99	23.642157516395333	29.39219085255719	27.880850496405756	19.084801134641726
100	30.191357460467668	40.67036943442284	29.138273105109498	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	188.0
1	225.0
2	338.5
3	465.0
4	578.0
5	747.0
6	929.5
7	1180.0
8	1420.0
9	1507.0
10	1501.5
11	1604.5
12	1856.5
13	2238.5
14	3117.5
15	4176.0
16	4777.5
17	4913.0
18	4788.5
19	4530.0
20	4255.0
21	4292.0
22	4797.5
23	5864.0
24	7618.5
25	9816.5
26	12841.0
27	16693.5
28	20741.5
29	25309.0
30	30256.5
31	35190.5
32	39997.0
33	45048.5
34	50241.0
35	55258.5
36	60376.0
37	65336.0
38	69334.5
39	72370.5
40	75858.5
41	78923.5
42	80896.5
43	82876.5
44	85095.5
45	87330.0
46	88632.0
47	89021.0
48	88525.0
49	87207.0
50	85354.0
51	82478.5
52	79040.0
53	74886.5
54	70002.0
55	65153.5
56	60095.0
57	54653.0
58	48491.0
59	41085.5
60	33748.0
61	27246.0
62	21565.5
63	16677.0
64	12594.0
65	9525.5
66	7150.0
67	5467.0
68	4068.5
69	2965.0
70	2237.5
71	1683.5
72	1259.0
73	886.0
74	603.5
75	409.5
76	257.0
77	156.0
78	106.5
79	76.5
80	52.5
81	31.0
82	18.5
83	12.5
84	8.0
85	4.5
86	3.5
87	3.0
88	2.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0019943258871682004
3	0.0
4	0.0
5	0.0038352420907080778
6	2.556828060472052E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	1.0622801578017174E-4
44-45	5.358986444175841E-5
46-47	0.0
48-49	0.0
50-51	2.75980759725355E-5
52-53	0.0010859909946285215
54-55	0.002191520333515679
56-57	0.0023260794639805176
58-59	0.0018047356262833676
60-61	0.004513228097969452
62-63	0.001986193037517142
64-65	0.0018279169500675003
66-67	4.1685834706732707E-4
68-69	2.1058946096017364E-4
70-71	2.4320145531750862E-4
72-73	0.0
74-75	0.0
76-77	0.0
78-79	9.493652860152797E-5
80-81	3.19847496713567E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	1.011022163627871E-4
92-93	1.7038282977255937E-4
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	38.0
20-21	84.0
22-23	115.0
24-25	171.0
26-27	609.0
28-29	2161.0
30-31	5188.0
32-33	6231.0
34-35	8732.0
36-37	13961.0
38-39	16418.0
40-41	15251.0
42-43	16061.0
44-45	18314.0
46-47	18425.0
48-49	17940.0
50-51	16372.0
52-53	15701.0
54-55	16821.0
56-57	17307.0
58-59	17155.0
60-61	16690.0
62-63	15934.0
64-65	16301.0
66-67	17268.0
68-69	17290.0
70-71	16951.0
72-73	15790.0
74-75	15786.0
76-77	16340.0
78-79	16694.0
80-81	16552.0
82-83	15480.0
84-85	15121.0
86-87	16021.0
88-89	16425.0
90-91	16539.0
92-93	16165.0
94-95	19478.0
96-97	58805.0
98-99	446928.0
100-101	929935.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	90.8926509740587
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.17488137051446	84.68911971959496
2	5.242719928471078	9.530494252265274
3	0.980965611303242	2.674876947772191
4	0.3053506712543696	1.1101652794807182
5	0.12028210385377337	0.5466379642003252
6	0.06655193100179504	0.36294488617174764
7	0.03263401385791687	0.2076334422029163
8	0.01974663505988126	0.14358592067279186
9	0.014663958096818802	0.11995614226731363
>10	0.041266041244471446	0.5549878962307959
>50	8.668049548525201E-4	0.05018485476488653
>100	7.093038736820594E-5	0.009412694376069163
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	5.1136561209441033E-5	0.0	0.0	0.0
3	0.0	5.1136561209441033E-5	0.0	0.0	0.0
4	0.0	5.1136561209441033E-5	0.0	0.0	0.0
5	0.0	5.1136561209441033E-5	0.0	0.0	0.0
6	0.0	5.1136561209441033E-5	0.0	0.0	0.0
7	0.0	5.1136561209441033E-5	0.0	0.0	0.0
8	0.0	5.1136561209441033E-5	0.0	0.0	0.0
9	5.1136561209441033E-5	1.0227312241888207E-4	0.0	0.0	0.0
10-11	5.1136561209441033E-5	1.0227312241888207E-4	0.0	5.1136561209441033E-5	0.0
12-13	5.1136561209441033E-5	1.0227312241888207E-4	0.0	5.1136561209441033E-5	0.0
14-15	5.1136561209441033E-5	2.0454624483776413E-4	0.0	1.0227312241888207E-4	0.0
16-17	5.1136561209441033E-5	2.0454624483776413E-4	0.0	1.534096836283231E-4	0.0
18-19	5.1136561209441033E-5	2.812510866519257E-4	0.0	1.7897796423304362E-4	0.0
20-21	5.1136561209441033E-5	3.5795592846608724E-4	0.0	2.0454624483776413E-4	0.0
22-23	5.1136561209441033E-5	4.0909248967552827E-4	0.0	2.3011452544248467E-4	0.0
24-25	5.1136561209441033E-5	4.0909248967552827E-4	0.0	2.556828060472052E-4	0.0
26-27	5.1136561209441033E-5	4.0909248967552827E-4	0.0	2.556828060472052E-4	0.0
28-29	5.1136561209441033E-5	4.0909248967552827E-4	0.0	5.625021733038514E-4	0.0
30-31	5.1136561209441033E-5	5.113656120944104E-4	0.0	0.001866484484144598	0.0
32-33	5.1136561209441033E-5	5.113656120944104E-4	0.0	0.004321039422197768	0.0
34-35	5.1136561209441033E-5	6.647752957227335E-4	0.0	0.00841196431895305	0.0
36-37	5.1136561209441033E-5	8.437532599557771E-4	0.0	0.0138324398071538	0.0
38-39	5.1136561209441033E-5	8.693215405604977E-4	0.0	0.02326713535029567	0.0
40-41	5.1136561209441033E-5	9.204581017699387E-4	0.0	0.03341774275036972	0.0
42-43	5.1136561209441033E-5	9.460263823746593E-4	0.0	0.04259675548746438	0.0
44-45	5.1136561209441033E-5	9.715946629793797E-4	0.0	0.05075303700037023	0.0
46-47	1.278414030236026E-4	9.715946629793797E-4	0.0	0.05957409380899881	0.0
48-49	1.534096836283231E-4	9.715946629793797E-4	0.0	0.06885537966851235	0.0
50-51	1.534096836283231E-4	0.0010738677853982617	0.0	0.07931280643584304	0.0
52-53	1.534096836283231E-4	0.0010738677853982617	0.0	0.08841511433112355	0.0
54-55	1.534096836283231E-4	0.0010738677853982617	0.0	0.09805435611910318	0.0
56-57	2.0454624483776416E-4	0.0011250043466077028	0.0	0.10651745699926568	0.0
58-59	2.556828060472052E-4	0.0011250043466077028	0.0	0.11406009977765824	0.0
60-61	2.556828060472052E-4	0.0011250043466077028	0.0	0.12198626676512159	0.0
62-63	2.556828060472052E-4	0.0011250043466077028	0.0	0.13029595796165577	0.0
64-65	2.556828060472052E-4	0.001278414030236026	0.0	0.13742950825037278	0.0
66-67	2.556828060472052E-4	0.001278414030236026	0.0	0.14364260043731986	0.0
68-69	2.812510866519257E-4	0.001278414030236026	0.0	0.1496511463794292	0.0
70-71	3.068193672566462E-4	0.001278414030236026	0.0	0.15519946327065354	0.0
72-73	3.068193672566462E-4	0.001278414030236026	0.0	0.1595205026928513	0.0
74-75	3.068193672566462E-4	0.0013039823108407463	0.0	0.16281881089086025	0.0
76-77	3.068193672566462E-4	0.0013551188720501875	0.0	0.16637280189491643	0.0
78-79	3.068193672566462E-4	0.001380687152654908	0.0	0.1682137180984563	0.0
80-81	3.3238764786136673E-4	0.001380687152654908	0.0	0.16892962995538846	0.0
82-83	3.5795592846608724E-4	0.0014062554332596286	0.0	0.16954326868990177	0.0
84-85	3.5795592846608724E-4	0.001431823713864349	0.0	0.16987565633776314	0.0
86-87	4.0909248967552827E-4	0.001431823713864349	0.0	0.17002906602139145	0.0
88	4.0909248967552827E-4	0.001534096836283231	0.0	0.17002906602139145	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1850	0.0	41.537624	1
GTATCAA	3140	0.0	33.29906	1
ATCAACG	3715	0.0	28.59577	3
CAACGCA	3780	0.0	28.104044	5
TCAACGC	3790	0.0	28.02989	4
AACGCAG	3935	0.0	26.890316	6
TATCAAC	4100	0.0	26.526392	2
ACGCAGA	4520	0.0	23.223656	7
CGCAGAG	4670	0.0	22.477713	8
TACATGG	5180	0.0	20.91473	2
ACATGGG	5225	0.0	20.331728	3
ACGCGAT	105	0.004491781	19.994461	8
GTACATG	5070	0.0	19.960499	1
TATACCG	155	1.0091234E-4	18.962975	5
CATGGGG	3825	0.0	18.881527	4
GCAGAGT	5845	0.0	17.959095	9
TAGACCG	165	1.6293357E-4	17.813702	5
CTATACG	215	3.5110297E-6	17.576975	4
ATACGAC	120	0.009706772	17.4956	3
TACGCGA	120	0.009708201	17.495152	7
>>END_MODULE