FastQCFastQC Report
Fri 27 May 2016
SRR2140295_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140295_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8558086
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87400.038.4733471
GTATCAA147700.031.1464231
TCAACGC154700.028.56364
ATCAACG155850.028.3793073
CAACGCA158350.027.8530925
AACGCAG164200.026.9863996
TATCAAC168550.026.5836662
TATACCG5100.023.4607375
ACGCAGA196150.022.5907047
CGCAGAG200000.022.0939458
GTACATG221900.021.1591761
TACATGG223100.020.5275552
CGTCGAA3406.7422996E-519.9858594
ACATGGG228000.019.7606663
GCAGAGT249450.017.7141279
CATGGGG181750.017.2298364
GAGTACT122250.017.21335612-13
AGAGTAC214850.016.35194610-11
CAGAGTA239200.015.94651910-11
GTGGTAT50450.015.6201791
Produced by FastQC (version 0.11.5)