##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140295_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8558086
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.07005947357855	33.0	31.0	34.0	30.0	34.0
2	32.20619925997472	33.0	31.0	34.0	30.0	34.0
3	32.11483946293599	33.0	31.0	34.0	30.0	34.0
4	35.989320626130656	37.0	35.0	37.0	35.0	37.0
5	35.859698885942485	37.0	35.0	37.0	35.0	37.0
6	35.915049463162674	37.0	35.0	37.0	35.0	37.0
7	35.86733423805276	37.0	35.0	37.0	35.0	37.0
8	35.85955492852023	37.0	35.0	37.0	35.0	37.0
9	37.62020257800634	39.0	37.0	39.0	35.0	39.0
10-11	37.50019636400008	39.0	37.0	39.0	35.0	39.0
12-13	37.47770926817047	39.0	37.0	39.0	34.5	39.0
14-15	38.78336184048629	40.0	38.0	41.0	35.0	41.0
16-17	38.63094645227917	40.0	38.0	41.0	34.0	41.0
18-19	38.6906862118469	40.0	38.0	41.0	34.0	41.0
20-21	38.71498700870431	40.0	38.0	41.0	34.0	41.0
22-23	38.640019661139334	40.0	38.0	41.0	34.0	41.0
24-25	38.58430285616142	40.0	38.0	41.0	34.0	41.0
26-27	38.379973555868645	40.0	38.0	41.0	34.0	41.0
28-29	38.27263573509592	40.0	38.0	41.0	34.0	41.0
30-31	38.16045172020981	40.0	38.0	41.0	33.5	41.0
32-33	37.98629372922585	40.0	37.0	41.0	33.0	41.0
34-35	38.08295525603141	40.0	38.0	41.0	33.0	41.0
36-37	38.060138603581805	40.0	37.5	41.0	33.0	41.0
38-39	38.01513127164806	40.0	37.0	41.0	33.0	41.0
40-41	37.946197272908215	40.0	37.0	41.0	33.0	41.0
42-43	38.01260290962399	40.0	37.0	41.0	33.0	41.0
44-45	38.01670752423517	40.0	37.0	41.0	33.0	41.0
46-47	37.95281955237126	40.0	37.0	41.0	33.0	41.0
48-49	37.86079755902123	40.0	37.0	41.0	33.0	41.0
50-51	37.74193284718743	40.0	37.0	41.0	32.5	41.0
52-53	37.56967880860038	40.0	36.0	41.0	32.0	41.0
54-55	37.41645365574327	39.0	36.0	41.0	32.0	41.0
56-57	37.274976457860646	39.0	36.0	41.0	31.0	41.0
58-59	37.10083520365794	39.0	35.0	41.0	31.0	41.0
60-61	36.9068703093146	39.0	35.0	40.0	31.0	41.0
62-63	36.68378897115628	38.0	35.0	40.0	31.0	41.0
64-65	36.3735897294313	38.0	35.0	40.0	30.5	41.0
66-67	36.06912904570633	37.0	34.5	40.0	30.0	41.0
68-69	35.749710393062784	37.0	34.0	39.5	30.0	41.0
70-71	35.427913624305596	36.0	34.0	39.0	29.0	41.0
72-73	35.13600787145078	36.0	34.0	39.0	29.0	40.0
74-75	34.80204042108369	35.0	34.0	38.5	29.0	40.0
76-77	33.540400201817576	34.5	32.0	36.5	28.0	39.0
78-79	34.23520356665655	35.0	33.0	37.0	29.0	39.0
80-81	34.145343085949975	35.0	33.5	36.5	29.0	39.0
82-83	33.92186349938085	35.0	33.0	36.0	29.0	37.5
84-85	33.68983426653173	35.0	33.0	36.0	29.0	37.0
86-87	33.49583815165046	35.0	33.0	35.0	29.0	37.0
88-89	33.331577432322284	35.0	33.0	35.0	29.0	36.0
90-91	33.20963160147612	35.0	33.0	35.0	29.0	36.0
92-93	33.1614221214084	35.0	33.0	35.0	29.0	36.0
94-95	33.09466809395293	35.0	33.0	35.0	29.0	35.5
96-97	33.13360680943376	35.0	33.0	35.0	29.0	35.0
98-99	33.250345819182414	35.0	33.0	35.0	29.0	35.0
100	32.00646340979741	34.0	31.0	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	4.0
19	10.0
20	51.0
21	247.0
22	1219.0
23	4342.0
24	11742.0
25	25300.0
26	46498.0
27	74207.0
28	108763.0
29	144551.0
30	184889.0
31	229218.0
32	284131.0
33	357661.0
34	474038.0
35	651834.0
36	1000975.0
37	1688863.0
38	2100100.0
39	1126327.0
40	43115.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.93045033667575	19.96010556566036	13.498625744120824	24.61081835354307
2	15.214593543462874	21.055221926958904	40.09193177072537	23.638252758852857
3	18.711957632439233	25.16015025373166	30.199156186970942	25.928735926858167
4	12.977773301179726	17.26667621708873	37.990141720940876	31.76540876079067
5	13.466994839734026	37.6518885180635	35.538156545751	13.342960096451472
6	31.407010866682107	37.14711443656911	18.078236185053527	13.367638511695255
7	27.568711041230483	32.05595269783454	22.550158995831545	17.825177265103438
8	25.180081153659827	36.50785935079409	20.66465562510122	17.647403870444865
9	25.73085851205515	16.462769829609098	20.91092564388813	36.89544601444762
10-11	24.844480413026933	26.39520682545139	28.538787761656053	20.221524999865625
12-13	25.204607665779477	23.78562800140125	28.835197496262598	22.17456683655668
14-15	22.27026580476055	25.35278916337134	26.080650509938785	26.29629452192932
16-17	22.196580220337502	28.530940175034903	26.862380057507547	22.41009954712005
18-19	22.194272287544337	27.940412458303935	28.326617403007937	21.53869785114379
20-21	22.870245716676855	27.130186638291715	28.132829495676624	21.866738149354802
22-23	22.727296897035405	26.9945736757978	27.972054123588602	22.306075303578197
24-25	22.53537030176334	27.114212707423686	28.004996280316995	22.34542071049598
26-27	22.510393855204143	27.238674818764995	27.93206239078931	22.31886893524155
28-29	22.43199271632125	27.3439879369922	27.937559774526612	22.286459572159938
30-31	22.590208554309182	27.17713377092413	27.91376291507119	22.318894759695507
32-33	22.35632183232225	27.325219113681587	27.92954636562272	22.388912688373445
34-35	22.554261323575563	27.280597634966615	27.90002631986074	22.265114721597083
36-37	22.566307428334095	27.240796939411467	27.800041105790896	22.392854526463545
38-39	22.481576526180756	27.36722258836713	27.890532450515064	22.26066843493705
40-41	22.595864009621117	27.258464456840425	27.774745995172896	22.370925538365565
42-43	22.47499307183456	27.448891597162508	27.595519985486515	22.48059534551642
44-45	22.647443588461226	27.397376453864364	27.553368924934894	22.40181103273951
46-47	22.5799502000589	27.40930593645028	27.405200279134856	22.605543584355964
48-49	22.49101566420576	27.605504633976093	27.34511184024538	22.558367861572773
50-51	22.50575682283508	27.53181093671495	27.379883808741507	22.582548431708464
52-53	22.628882106233263	27.50239855754647	27.213098684208838	22.65562065201143
54-55	22.6349682756642	27.403208258189792	27.329057682819535	22.63276578332647
56-57	22.649275959791986	27.47301168526386	27.27873352394612	22.598978830998036
58-59	22.52097617278947	27.604326538859915	27.309096119305902	22.565601169044708
60-61	22.65399277547094	27.54690577012542	27.311065426602276	22.488036027801368
62-63	22.701534123744455	27.614247772766877	27.16226833821483	22.52194976527384
64-65	22.602879887924345	27.566612707925415	27.19732678039305	22.63318062375719
66-67	22.68901644050686	27.532366603179515	27.097088660677215	22.681528295636404
68-69	22.510299719028573	27.547962631932354	27.220749792964906	22.720987856074167
70-71	22.516804273127775	27.43228770643727	27.244278568403118	22.80662945203183
72-73	22.634851221352513	27.485170823983317	27.14949226871916	22.730485685945006
74-75	22.506363219298855	27.61073459153507	27.183352178544006	22.699550010622065
76-77	22.622816679036912	27.513061775076736	27.116405111552353	22.747716434333995
78-79	22.494463208482465	27.50354649653939	27.138788533959175	22.863201761018974
80-81	22.561886406139568	27.529902690767273	27.16044561587771	22.74776528721545
82-83	22.48906150988797	27.623060555275636	27.28966745317455	22.598210481661845
84-85	22.629431860928957	27.583240248392006	27.15928754240907	22.628040348269966
86-87	22.49887650581919	27.55797809357798	27.25843219727399	22.684713203328837
88-89	22.553720768669777	27.509589807957386	27.199094319476114	22.73759510389672
90-91	22.5272228503226	27.59763887765828	27.197510282036685	22.677627989982426
92-93	22.60284127456637	27.511468682086793	27.287321442977465	22.598368600369376
94-95	22.352054035949955	27.646411274614962	27.353678777409744	22.647855912025346
96-97	22.354784585313894	27.725609745484668	27.496740887975314	22.422864781226124
98-99	23.5702467482785	29.710453328232596	27.540311782708493	19.17898814078041
100	30.289157582197863	41.23168575191502	28.479156665887118	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1198.0
1	1091.5
2	1505.0
3	2151.5
4	2803.0
5	4465.5
6	5978.0
7	7017.5
8	7731.0
9	7671.5
10	7099.0
11	6397.5
12	6694.0
13	8617.5
14	13229.5
15	19604.0
16	25220.5
17	29124.0
18	30906.5
19	31001.5
20	30334.0
21	29650.0
22	30338.5
23	33920.5
24	40521.0
25	50370.0
26	63512.0
27	78585.0
28	94006.5
29	111779.5
30	131044.5
31	151548.0
32	174341.5
33	196344.5
34	217098.5
35	236795.5
36	259620.5
37	282057.5
38	297401.5
39	306848.0
40	318459.5
41	333433.5
42	345014.5
43	356291.0
44	366765.0
45	374279.0
46	379119.0
47	383358.0
48	386363.5
49	384934.0
50	378196.5
51	367703.5
52	354112.5
53	338151.0
54	320691.0
55	299572.5
56	276422.0
57	252607.5
58	226410.0
59	195550.5
60	164143.5
61	135473.0
62	109527.0
63	86279.5
64	66305.0
65	51000.5
66	39034.0
67	30254.0
68	23211.0
69	17308.0
70	12870.5
71	9454.0
72	6804.5
73	4669.5
74	3295.0
75	2324.5
76	1563.0
77	996.5
78	651.0
79	465.0
80	337.0
81	222.5
82	154.5
83	108.0
84	72.0
85	52.5
86	40.5
87	29.0
88	18.0
89	8.5
90	3.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	3.3886081537390486E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	9.172611726500527E-4
18-19	0.003785893247625696
20-21	0.0
22-23	0.0013965146966518824
24-25	4.733960203174559E-4
26-27	0.013301008666644916
28-29	0.007214113122208481
30-31	6.916437593862799E-4
32-33	0.0069492539840625735
34-35	0.004721887050218862
36-37	0.001976885870460546
38-39	0.0013947232650555438
40-41	0.0037688653909258647
42-43	0.00819242544435679
44-45	0.009853648682936682
46-47	0.009045334130718159
48-49	0.008158463955906243
50-51	0.033569132291416746
52-53	0.012656915525121791
54-55	1.2994038205331012E-4
56-57	0.008177459139305676
58-59	0.00658238468837689
60-61	1.0075479446727211E-4
62-63	0.005167579300103331
64-65	0.0025879206572357438
66-67	0.023966043359718435
68-69	0.03167545661759408
70-71	0.015278968197827555
72-73	0.01136421413290542
74-75	0.016373156999703143
76-77	0.002138901281814036
78-79	0.0029349454772266154
80-81	0.001657375525422097
82-83	0.024093513207019197
84-85	0.0010183592289388153
86-87	0.009852512615354204
88-89	9.042000090420001E-4
90-91	0.0029203238842552813
92-93	0.00115128621447402
94-95	8.201854227852923E-4
96-97	0.0029326796851537745
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	195.0
20-21	612.0
22-23	1350.0
24-25	3044.0
26-27	4741.0
28-29	11946.0
30-31	24702.0
32-33	30830.0
34-35	43077.0
36-37	65440.0
38-39	75661.0
40-41	74641.0
42-43	80539.0
44-45	91736.0
46-47	91204.0
48-49	85760.0
50-51	79224.0
52-53	77098.0
54-55	82731.0
56-57	84707.0
58-59	84304.0
60-61	81831.0
62-63	78681.0
64-65	82577.0
66-67	86956.0
68-69	87117.0
70-71	84375.0
72-73	80739.0
74-75	82904.0
76-77	82397.0
78-79	87752.0
80-81	89074.0
82-83	86673.0
84-85	87046.0
86-87	93116.0
88-89	100040.0
90-91	107774.0
92-93	119129.0
94-95	145307.0
96-97	326811.0
98-99	1818280.0
100-101	3655965.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.7364348063511
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.26343808846413	77.38865375068713
2	6.728329864170063	11.537260295100834
3	1.6933961203059387	4.355572382098139
4	0.6047689709564702	2.074029418052538
5	0.2737523537056844	1.1735275413286288
6	0.14344978656323706	0.7379323964198371
7	0.08891541934516181	0.5336303737776084
8	0.052088875575324975	0.3572731587919987
9	0.03688289752071759	0.28459873248788986
>10	0.11335924792275685	1.4404333186474756
>50	0.0013849113039326624	0.07862382823790985
>100	2.118430603691902E-4	0.028565957934827704
>500	2.1621106185042077E-5	0.00989884643507279
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1684855702548443E-5	0.0	0.0	0.0	0.0
2	1.1684855702548443E-5	0.0	0.0	0.0	1.1684855702548443E-5
3	1.1684855702548443E-5	0.0	0.0	0.0	1.1684855702548443E-5
4	1.1684855702548443E-5	5.8424278512742215E-5	0.0	0.0	1.1684855702548443E-5
5	2.3369711405096886E-5	5.8424278512742215E-5	0.0	0.0	1.1684855702548443E-5
6	2.3369711405096886E-5	5.8424278512742215E-5	0.0	0.0	2.3369711405096886E-5
7	2.3369711405096886E-5	5.8424278512742215E-5	0.0	0.0	2.3369711405096886E-5
8	2.3369711405096886E-5	5.8424278512742215E-5	0.0	0.0	3.505456710764533E-5
9	2.3369711405096886E-5	1.1684855702548443E-4	0.0	1.1684855702548443E-5	4.673942281019377E-5
10-11	8.17939899178391E-5	1.1684855702548443E-4	0.0	1.1684855702548443E-5	4.673942281019377E-5
12-13	1.0516370132293599E-4	1.1684855702548443E-4	0.0	1.1684855702548443E-5	4.673942281019377E-5
14-15	1.1684855702548443E-4	1.5190312413312977E-4	0.0	1.1684855702548443E-5	8.17939899178391E-5
16-17	1.285334127280329E-4	1.5774555198440397E-4	0.0	1.1684855702548443E-5	9.347884562038754E-5
18-19	1.285334127280329E-4	1.6943040768695243E-4	0.0	1.1684855702548443E-5	9.347884562038754E-5
20-21	1.4021826843058132E-4	1.7527283553822666E-4	0.0	1.1684855702548443E-5	9.347884562038754E-5
22-23	1.5774555198440397E-4	2.161698304971462E-4	0.0	2.3369711405096886E-5	1.0516370132293599E-4
24-25	1.7527283553822663E-4	2.512243976047915E-4	0.0	5.8424278512742215E-5	1.1684855702548443E-4
26-27	1.9280011909204932E-4	2.570668254560658E-4	0.0	9.932127347166177E-5	1.1684855702548443E-4
28-29	1.9864254694332354E-4	2.570668254560658E-4	1.1684855702548443E-5	5.141336509121314E-4	1.1684855702548443E-4
30-31	2.3369711405096886E-4	3.15491103968808E-4	1.1684855702548443E-5	0.002050692175797252	1.2269098487675866E-4
32-33	2.3369711405096886E-4	3.3886081537390486E-4	1.1684855702548443E-5	0.0047966332658961365	1.5190312413312977E-4
34-35	2.6290925330734E-4	3.914426660353729E-4	1.1684855702548443E-5	0.008389726394429782	1.635879798356782E-4
36-37	2.921213925637111E-4	4.323396609942924E-4	1.1684855702548443E-5	0.014121148116529793	1.635879798356782E-4
38-39	2.921213925637111E-4	4.4402451669684086E-4	1.1684855702548443E-5	0.022604353356579964	1.635879798356782E-4
40-41	3.0380624826625954E-4	4.790790838044862E-4	1.1684855702548443E-5	0.03198145005787509	1.635879798356782E-4
42-43	3.0380624826625954E-4	5.02448795209583E-4	1.1684855702548443E-5	0.040745091834786425	1.6943040768695243E-4
44-45	3.0380624826625954E-4	5.78400357276148E-4	1.1684855702548443E-5	0.05032667351087615	1.811152633895009E-4
46-47	3.0380624826625954E-4	6.30982207937616E-4	1.1684855702548443E-5	0.06157334712457902	1.869576912407751E-4
48-49	3.0380624826625954E-4	6.30982207937616E-4	1.1684855702548443E-5	0.07192028684918567	1.869576912407751E-4
50-51	3.15491103968808E-4	6.835640585990839E-4	1.1684855702548443E-5	0.08180567477354164	1.869576912407751E-4
52-53	3.15491103968808E-4	6.894064864503581E-4	1.1684855702548443E-5	0.09162095356368236	2.1032740264587197E-4
54-55	3.15491103968808E-4	6.894064864503581E-4	1.1684855702548443E-5	0.10147712934878195	2.3369711405096886E-4
56-57	3.15491103968808E-4	6.894064864503581E-4	1.1684855702548443E-5	0.11027582569280095	2.3369711405096886E-4
58-59	3.15491103968808E-4	6.952489143016323E-4	1.1684855702548443E-5	0.11870644908218964	2.512243976047915E-4
60-61	3.271759596713564E-4	7.010913421529066E-4	1.1684855702548443E-5	0.12648272055223564	2.6875168115861423E-4
62-63	3.271759596713564E-4	7.186186257067293E-4	1.1684855702548443E-5	0.1339201312069077	2.8043653686116263E-4
64-65	3.271759596713564E-4	7.828853320707458E-4	1.1684855702548443E-5	0.14143349342364636	2.8043653686116263E-4
66-67	3.271759596713564E-4	7.945701877732942E-4	1.1684855702548443E-5	0.1491747103265847	2.8627896471243683E-4
68-69	3.3886081537390486E-4	8.120974713271168E-4	1.1684855702548443E-5	0.15698019393588708	2.921213925637111E-4
70-71	3.3886081537390486E-4	8.237823270296653E-4	1.1684855702548443E-5	0.16349450099005783	2.921213925637111E-4
72-73	3.3886081537390486E-4	8.296247548809395E-4	1.1684855702548443E-5	0.16844887980793838	3.15491103968808E-4
74-75	3.3886081537390486E-4	8.41309610583488E-4	1.1684855702548443E-5	0.1719368092351491	3.15491103968808E-4
76-77	3.3886081537390486E-4	8.70521749839859E-4	1.1684855702548443E-5	0.1756233812093031	3.15491103968808E-4
78-79	3.3886081537390486E-4	8.938914612449559E-4	1.1684855702548443E-5	0.1780304614840281	3.15491103968808E-4
80-81	3.3886081537390486E-4	9.055763169475044E-4	1.1684855702548443E-5	0.17880750438824755	3.15491103968808E-4
82-83	3.3886081537390486E-4	9.114187447987786E-4	1.1684855702548443E-5	0.17924568647709313	3.505456710764533E-4
84-85	3.3886081537390486E-4	9.114187447987786E-4	1.1684855702548443E-5	0.17941511688478007	3.505456710764533E-4
86-87	3.3886081537390486E-4	9.23103600501327E-4	1.1684855702548443E-5	0.17949106844684665	3.5638809892772757E-4
88	3.3886081537390486E-4	9.46473311906424E-4	1.1684855702548443E-5	0.1795027533025492	3.739153824815502E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8740	0.0	38.473347	1
GTATCAA	14770	0.0	31.146423	1
TCAACGC	15470	0.0	28.5636	4
ATCAACG	15585	0.0	28.379307	3
CAACGCA	15835	0.0	27.853092	5
AACGCAG	16420	0.0	26.986399	6
TATCAAC	16855	0.0	26.583666	2
TATACCG	510	0.0	23.460737	5
ACGCAGA	19615	0.0	22.590704	7
CGCAGAG	20000	0.0	22.093945	8
GTACATG	22190	0.0	21.159176	1
TACATGG	22310	0.0	20.527555	2
CGTCGAA	340	6.7422996E-5	19.98585	94
ACATGGG	22800	0.0	19.760666	3
GCAGAGT	24945	0.0	17.714127	9
CATGGGG	18175	0.0	17.229836	4
GAGTACT	12225	0.0	17.213356	12-13
AGAGTAC	21485	0.0	16.351946	10-11
CAGAGTA	23920	0.0	15.946519	10-11
GTGGTAT	5045	0.0	15.620179	1
>>END_MODULE