FastQCFastQC Report
Fri 27 May 2016
SRR2140294_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140294_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8544521
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91350.046.932831
GTATCAA160800.034.0410041
ATCAACG182400.029.3178353
TCAACGC183100.029.2287274
CAACGCA185150.028.8369335
AACGCAG193550.027.6071596
TATCAAC199250.027.1341322
ACGCAGA227100.023.5472167
CGCAGAG229850.023.2654918
GTACATG222350.021.2119031
TACATGG215700.021.1831652
ACATGGG224850.019.9094773
GCAGAGT274450.019.3927089
GAGTACT160050.018.7450112-13
CATGGGG167400.018.3727044
CAGAGTA268650.017.5642110-11
AGAGTAC246450.017.0038310-11
GTACTTT184350.016.20666714-15
ATGGGGG112200.015.3370425
TACGCGG1650.003293354315.2995425
Produced by FastQC (version 0.11.5)