##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140294_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8544521
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.02142367020925	31.0	31.0	34.0	30.0	34.0
2	32.16163211489562	33.0	31.0	34.0	30.0	34.0
3	32.063142802270605	33.0	31.0	34.0	30.0	34.0
4	35.95404985253123	37.0	35.0	37.0	35.0	37.0
5	35.82021590209679	37.0	35.0	37.0	35.0	37.0
6	35.877949624092444	37.0	35.0	37.0	35.0	37.0
7	35.83174165058521	37.0	35.0	37.0	35.0	37.0
8	35.823849458617985	37.0	35.0	37.0	35.0	37.0
9	37.573031887919754	39.0	37.0	39.0	35.0	39.0
10-11	37.45373649383038	39.0	37.0	39.0	34.5	39.0
12-13	37.429242317971955	39.0	37.0	39.0	34.0	39.0
14-15	38.71382000231493	40.0	38.0	41.0	34.0	41.0
16-17	38.560420589989775	40.0	38.0	41.0	34.0	41.0
18-19	38.6303542937047	40.0	38.0	41.0	34.0	41.0
20-21	38.65890481569012	40.0	38.0	41.0	34.0	41.0
22-23	38.58330004780993	40.0	38.0	41.0	34.0	41.0
24-25	38.52721860727151	40.0	38.0	41.0	34.0	41.0
26-27	38.318980464012874	40.0	38.0	41.0	34.0	41.0
28-29	38.207019179129986	40.0	38.0	41.0	33.5	41.0
30-31	38.08387622679959	40.0	37.5	41.0	33.0	41.0
32-33	37.900505135921065	40.0	37.0	41.0	33.0	41.0
34-35	37.99319143344778	40.0	37.0	41.0	33.0	41.0
36-37	37.968760296663206	40.0	37.0	41.0	33.0	41.0
38-39	37.92442779253115	40.0	37.0	41.0	33.0	41.0
40-41	37.85470287331539	40.0	37.0	41.0	33.0	41.0
42-43	37.91883847383593	40.0	37.0	41.0	33.0	41.0
44-45	37.92476664829411	40.0	37.0	41.0	33.0	41.0
46-47	37.86401622836435	40.0	37.0	41.0	33.0	41.0
48-49	37.77406115956613	40.0	37.0	41.0	33.0	41.0
50-51	37.65680092535122	40.0	37.0	41.0	32.0	41.0
52-53	37.486010678620104	39.5	36.0	41.0	32.0	41.0
54-55	37.341365752850635	39.0	36.0	41.0	31.0	41.0
56-57	37.208661238139754	39.0	36.0	41.0	31.0	41.0
58-59	37.04469952194842	39.0	35.0	41.0	31.0	41.0
60-61	36.86166338475661	39.0	35.0	40.0	31.0	41.0
62-63	36.654005734806574	38.0	35.0	40.0	31.0	41.0
64-65	36.354187281702565	38.0	35.0	40.0	30.5	41.0
66-67	36.064442133284956	37.5	34.5	40.0	30.0	41.0
68-69	35.754163744570405	37.0	34.0	39.5	29.5	41.0
70-71	35.449929290790514	36.0	34.0	39.0	29.0	41.0
72-73	35.167026056485064	36.0	34.0	39.0	29.0	40.5
74-75	34.83937732519593	35.5	34.0	38.5	29.0	40.0
76-77	33.567398718095404	34.5	31.5	36.5	27.5	39.0
78-79	34.28145790131852	35.0	33.0	37.0	29.0	39.0
80-81	34.19422669307065	35.0	33.5	37.0	29.0	39.0
82-83	33.962062559004266	35.0	33.0	36.0	29.0	38.5
84-85	33.71873530323728	35.0	33.0	36.0	29.0	37.0
86-87	33.511588068254106	35.0	33.0	35.5	29.0	37.0
88-89	33.336647578391734	35.0	33.0	35.0	29.0	36.5
90-91	33.2024992027527	35.0	33.0	35.0	29.0	36.0
92-93	33.14290110337303	35.0	33.0	35.0	29.0	36.0
94-95	33.072297150962335	35.0	33.0	35.0	29.0	36.0
96-97	33.10914952022555	35.0	33.0	35.0	29.0	35.0
98-99	33.22616379467743	34.0	33.0	35.0	29.0	35.0
100	31.978596483498674	34.0	31.0	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	12.0
20	39.0
21	299.0
22	1392.0
23	4934.0
24	12982.0
25	27743.0
26	50320.0
27	79341.0
28	113639.0
29	151670.0
30	192026.0
31	236934.0
32	292395.0
33	368577.0
34	486828.0
35	665898.0
36	1016974.0
37	1694971.0
38	2076278.0
39	1032134.0
40	39133.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.25910627406733	20.646505520906324	13.940055855676404	24.154332349349954
2	14.87946486409244	21.418895219521374	40.70809820702647	22.993541709359715
3	18.200201112108765	25.997368127850372	30.647067442777143	25.155363317263713
4	12.628841335868914	17.896930676394852	38.92724940344813	30.54697858428811
5	13.000588330229396	37.98542949335603	35.988430480772415	13.025551695642154
6	30.169192632331292	38.1292994657044	18.589561661794736	13.111946240169578
7	26.579874986555712	32.58352340640277	23.27594490083177	17.560656706209745
8	24.463922553411713	36.92775756534509	21.19528994077023	17.413029940472967
9	25.264084434926193	16.769600074714543	21.550874531176177	36.41544095918308
10-11	24.237988296827872	26.920408996595597	28.940288168289364	19.901314538287167
12-13	24.70341520607182	24.289974827143617	29.3117016155733	21.69490835121126
14-15	21.752688067593258	25.922143558427674	26.766860307324425	25.558308066654646
16-17	21.61778982460514	29.06631626315842	27.4235452665975	21.892348645638947
18-19	21.5128048926786	28.376043617918363	29.072340928548563	21.038810560854476
20-21	22.24548682886454	27.57382364252581	28.98645211835752	21.194237410252132
22-23	21.978179931754934	27.477085042269245	28.79928056139247	21.745454464583357
24-25	21.778432460312068	27.551006729520637	28.93710265163402	21.733458158533274
26-27	21.821831119961345	27.76572323820958	28.784297992591185	21.62814764923789
28-29	21.697042929153827	27.9262355507165	28.738026564530855	21.638694955598815
30-31	21.95152416311512	27.74131287043044	28.701505887637317	21.60565707881712
32-33	21.774345234052976	27.80571496775449	28.663992010722144	21.755947787470394
34-35	21.882728541675572	27.848830336520532	28.699118422623567	21.56932269918033
36-37	21.861242059254593	27.8238154882123	28.569600352316932	21.745342100216174
38-39	21.751072807063547	27.921294603364828	28.647730037341763	21.679902552229866
40-41	22.002208741796	27.819464773719595	28.491660843467614	21.68666564101679
42-43	21.89277191420923	27.895141663665967	28.35391709762528	21.858169324499528
44-45	21.9497850595297	28.009382516179993	28.221093131381625	21.819739292908686
46-47	21.955055696925236	27.989317977414274	27.992160377677028	22.06346594798346
48-49	21.93527075752783	28.030197431016607	28.007919642356	22.026612169099565
50-51	21.901628077806183	28.17532547524796	27.935411640727253	21.987634806218608
52-53	22.03393247106534	28.09453337765558	27.82644552663124	22.045088624647843
54-55	22.022083499947414	28.019796547061894	27.8532099906459	22.104909962344795
56-57	22.040238925430415	28.149427877898397	27.83062432037504	21.97970887629614
58-59	21.960967058772674	28.221323600120467	27.87016380338727	21.947545537719588
60-61	22.05974838349631	28.17653208886992	27.83405722807588	21.929662299557886
62-63	22.03754324733315	28.27282549727052	27.71978479270672	21.96984646268961
64-65	21.984706500846407	28.19621733832195	27.8059017953942	22.01317436543744
66-67	22.05131986879602	28.202349912971798	27.664064743998356	22.08226547423383
68-69	22.04181868236428	28.15290761990788	27.768624559379806	22.036649138348032
70-71	21.976291958220433	27.992599176042148	27.800937977766665	22.230170887970747
72-73	22.092553484464542	28.02239484191026	27.771507292020303	22.113544381604896
74-75	21.914908817489277	28.20246352995485	27.731600251724547	22.15102740083132
76-77	22.011827142335985	28.11217191952002	27.68724913075229	22.18875180739171
78-79	21.964522984438677	28.0628757223943	27.72172810139332	22.25087319177371
80-81	21.974576960455362	28.127755198471483	27.733202556290728	22.164465284782427
82-83	21.96233403981637	28.169659331183215	27.813099460634493	22.054907168365915
84-85	22.11891924020625	28.084881589736533	27.74035792276852	22.055841247288697
86-87	21.932869018129757	28.14812672372358	27.810953358280667	22.108050899866
88-89	21.99648027507452	28.080192050187996	27.79959081304502	22.12373686169246
90-91	22.00669854097925	28.167830059690857	27.796831042200115	22.028640357129778
92-93	22.043997111342815	28.174084979735053	27.794712591897447	21.98720531702468
94-95	21.80902533103494	28.246175095327235	27.88505424948434	22.059745324153486
96-97	21.850790846554524	28.368864193782432	27.995869516949202	21.784475442713838
98-99	22.93826321833503	30.281222489481017	28.199311904877433	18.58120238730652
100	21.922746642676277	31.353404960959892	21.90671677165085	24.81713162471299
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1602.0
1	1450.0
2	1982.5
3	2866.5
4	3781.5
5	5954.5
6	7948.0
7	9309.5
8	10210.5
9	10099.5
10	9262.0
11	8357.5
12	8843.0
13	11608.5
14	18136.5
15	27308.0
16	35225.0
17	40168.5
18	42111.0
19	41699.5
20	40261.0
21	38992.0
22	39255.0
23	43012.0
24	50508.5
25	62071.0
26	78159.0
27	96216.5
28	115089.5
29	136837.5
30	156672.0
31	174887.5
32	195258.5
33	214554.5
34	233151.0
35	251011.5
36	270249.5
37	288143.0
38	300044.0
39	309866.0
40	321103.5
41	331559.5
42	338132.0
43	343157.0
44	347620.0
45	352754.0
46	356592.5
47	357026.5
48	355525.0
49	350864.0
50	344043.0
51	332741.5
52	316920.5
53	299243.0
54	280786.0
55	260798.0
56	239454.5
57	217326.5
58	192878.5
59	166227.5
60	138256.0
61	112181.5
62	89702.5
63	70065.0
64	52906.5
65	39660.5
66	30186.0
67	23191.5
68	17752.0
69	13163.0
70	9642.5
71	6994.0
72	4995.0
73	3558.5
74	2456.5
75	1637.0
76	1039.5
77	616.0
78	383.5
79	248.5
80	168.5
81	121.0
82	96.5
83	72.0
84	52.0
85	33.5
86	20.5
87	16.5
88	12.5
89	8.0
90	4.5
91	2.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	3.862124044168187E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0010064929327226184
18-19	0.00381531041939039
20-21	0.0
22-23	0.0012992426117866586
24-25	5.268340277883877E-4
26-27	0.013796652566675133
28-29	0.007429922006913343
30-31	6.45357812185388E-4
32-33	0.006978665742605877
34-35	0.00476362205776328
36-37	0.0020953894683831183
38-39	0.0015536256205127157
40-41	0.0038100961797675266
42-43	0.008476463395041385
44-45	0.009487939551607276
46-47	0.008323917770987415
48-49	0.007817678360145948
50-51	0.03406982809013278
52-53	0.012219250900713106
54-55	8.25659935696334E-5
56-57	0.007902960110433772
58-59	0.006464432388864015
60-61	1.306805102629612E-4
62-63	0.004868706232359599
64-65	0.0024383300690134016
66-67	0.02264341807748695
68-69	0.03044948809862148
70-71	0.014553469190966933
72-73	0.010302800634189974
74-75	0.015610572020388421
76-77	0.002016783630615317
78-79	0.0026038372049242958
80-81	0.001410349421742122
82-83	0.02294173126094509
84-85	7.204573102776988E-4
86-87	0.008889218344698163
88-89	8.268364210779614E-4
90-91	0.0029941195021462963
92-93	0.0010805932889393591
94-95	6.786556502048519E-4
96-97	0.003199472726894608
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	192.0
20-21	607.0
22-23	1442.0
24-25	3015.0
26-27	4414.0
28-29	8544.0
30-31	16862.0
32-33	21747.0
34-35	30234.0
36-37	44491.0
38-39	53318.0
40-41	56926.0
42-43	63511.0
44-45	74813.0
46-47	75165.0
48-49	70091.0
50-51	65243.0
52-53	64033.0
54-55	70496.0
56-57	73818.0
58-59	74592.0
60-61	73932.0
62-63	73107.0
64-65	77483.0
66-67	82275.0
68-69	83756.0
70-71	82834.0
72-73	79738.0
74-75	81764.0
76-77	82284.0
78-79	88617.0
80-81	90925.0
82-83	89269.0
84-85	91467.0
86-87	97182.0
88-89	105719.0
90-91	113532.0
92-93	126337.0
94-95	154496.0
96-97	334186.0
98-99	577806.0
100-101	5084258.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.43392149868106
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.95774039963793	74.2209312920344
2	7.391518679523122	12.334067785267337
3	2.0039236469169865	5.015856247386675
4	0.725447920565153	2.421078594232575
5	0.3550528366223178	1.4811725249315246
6	0.18980641066135204	0.9501775900239399
7	0.1082897435706302	0.6324526574928985
8	0.0726129741148219	0.4846708145665448
9	0.04531786846719435	0.34029427321615024
>10	0.147326759010106	1.8614652018210573
>50	0.0023491307418127935	0.13159764716784184
>100	5.655522376350155E-4	0.08742575162356803
>500	2.403896743768997E-5	0.01534333971410765
>1k	2.4038963599021572E-5	0.02346628052141588
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.1703406194449051E-5	0.0	0.0	0.0
3	0.0	1.1703406194449051E-5	0.0	0.0	0.0
4	0.0	2.3406812388898102E-5	0.0	2.3406812388898102E-5	0.0
5	0.0	3.5110218583347156E-5	0.0	2.3406812388898102E-5	0.0
6	8.192384336114335E-5	3.5110218583347156E-5	0.0	4.6813624777796204E-5	0.0
7	8.192384336114335E-5	3.5110218583347156E-5	0.0	4.6813624777796204E-5	0.0
8	9.362724955559241E-5	3.5110218583347156E-5	0.0	7.022043716669431E-5	0.0
9	9.362724955559241E-5	7.022043716669431E-5	0.0	1.5214428052783767E-4	0.0
10-11	1.0533065575004146E-4	7.022043716669431E-5	0.0	1.8725449911118482E-4	0.0
12-13	1.0533065575004146E-4	7.022043716669431E-5	0.0	2.34068123888981E-4	0.0
14-15	1.0533065575004146E-4	8.192384336114335E-5	0.0	2.4577153008343005E-4	0.0
16-17	2.3406812388898103E-4	8.192384336114335E-5	0.0	2.691783424723282E-4	0.0
18-19	2.3406812388898103E-4	9.362724955559241E-5	0.0	2.984368579584508E-4	0.0
20-21	2.3406812388898103E-4	1.0533065575004146E-4	0.0	3.276953734445734E-4	0.0
22-23	2.3406812388898103E-4	1.1703406194449052E-4	0.0	3.45250482736247E-4	0.0
24-25	2.3991982698620554E-4	1.2873746813893958E-4	0.0	3.8036070131959414E-4	0.0
26-27	2.4577153008343005E-4	1.4044087433338863E-4	0.0	4.56432841583513E-4	0.0
28-29	2.4577153008343005E-4	1.4044087433338863E-4	0.0	7.197594809586166E-4	0.0
30-31	2.4577153008343005E-4	1.638476867222867E-4	0.0	0.0019252103189868692	0.0
32-33	2.4577153008343005E-4	1.638476867222867E-4	0.0	0.0040084166215988	0.0
34-35	2.4577153008343005E-4	2.2821642079175647E-4	0.0	0.006612424499863713	0.0
36-37	2.5162323318065455E-4	2.9258515486122626E-4	0.0	0.010971943307295985	0.0
38-39	2.574749362778791E-4	2.9258515486122626E-4	0.0	0.01795887680538207	0.0
40-41	2.574749362778791E-4	3.45250482736247E-4	0.0	0.02492825519417648	0.0
42-43	2.691783424723282E-4	3.628055920279206E-4	0.0	0.03138853541351236	0.0
44-45	2.691783424723282E-4	4.330260291946149E-4	0.0	0.03904841476777926	0.0
46-47	2.808817486667772E-4	4.798396539724111E-4	0.0	0.047287612728671394	0.0
48-49	2.9258515486122626E-4	4.798396539724111E-4	0.0	0.05570236178248026	0.0
50-51	2.9258515486122626E-4	5.149498725557582E-4	0.0	0.06436873406946977	0.0
52-53	2.9258515486122626E-4	5.149498725557582E-4	0.0	0.07250845307770909	0.0
54-55	2.9258515486122626E-4	5.208015756529827E-4	0.0	0.08054284143019837	0.0
56-57	2.9258515486122626E-4	5.5591179423633E-4	0.0	0.08755903344377057	0.0
58-59	2.9258515486122626E-4	5.617634973335545E-4	0.0	0.09509602703299577	0.0
60-61	3.511021858334715E-4	5.67615200430779E-4	0.0	0.10187815092267899	0.0
62-63	3.979158106112677E-4	5.91022012819677E-4	0.0	0.10843205839157045	0.0
64-65	3.979158106112677E-4	6.319839345002488E-4	0.0	0.11463486367462845	0.0
66-67	4.096192168057168E-4	6.319839345002488E-4	0.0	0.12167446250058955	0.0
68-69	4.2132262300016585E-4	6.436873406946978E-4	0.0	0.12864384088938396	0.0
70-71	4.2717432609739036E-4	6.436873406946978E-4	0.0	0.13413273839458056	0.0
72-73	4.739879508751866E-4	6.436873406946978E-4	0.0	0.138913579825013	0.0
74-75	4.798396539724111E-4	6.612424499863713E-4	0.0	0.14239534316786162	0.0
76-77	4.798396539724111E-4	6.963526685697186E-4	0.0	0.1458536996983213	0.0
78-79	4.798396539724111E-4	7.256111840558411E-4	0.0	0.14814756731243334	0.0
80-81	4.915430601668601E-4	7.256111840558411E-4	0.0	0.14870933080976687	0.0
82-83	4.973947632640847E-4	7.314628871530656E-4	0.0	0.14922428068232263	0.0
84-85	5.208015756529827E-4	7.373145902502902E-4	0.0	0.14942323858762824	0.0
86-87	5.325049818474318E-4	7.431662933475148E-4	0.0	0.14951686583718385	0.0
88	5.383566849446564E-4	7.841282150280864E-4	0.0	0.14952271754028107	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9135	0.0	46.93283	1
GTATCAA	16080	0.0	34.041004	1
ATCAACG	18240	0.0	29.317835	3
TCAACGC	18310	0.0	29.228727	4
CAACGCA	18515	0.0	28.836933	5
AACGCAG	19355	0.0	27.607159	6
TATCAAC	19925	0.0	27.134132	2
ACGCAGA	22710	0.0	23.547216	7
CGCAGAG	22985	0.0	23.265491	8
GTACATG	22235	0.0	21.211903	1
TACATGG	21570	0.0	21.183165	2
ACATGGG	22485	0.0	19.909477	3
GCAGAGT	27445	0.0	19.392708	9
GAGTACT	16005	0.0	18.74501	12-13
CATGGGG	16740	0.0	18.372704	4
CAGAGTA	26865	0.0	17.56421	10-11
AGAGTAC	24645	0.0	17.00383	10-11
GTACTTT	18435	0.0	16.206667	14-15
ATGGGGG	11220	0.0	15.337042	5
TACGCGG	165	0.0032933543	15.299542	5
>>END_MODULE