FastQCFastQC Report
Fri 27 May 2016
SRR2140293_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140293_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8225971
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104500.040.9120141
GTATCAA169250.030.8792251
ATCAACG180850.027.8049963
TCAACGC190200.026.4379754
CAACGCA189500.026.4246085
AACGCAG193800.025.9034446
ACGCAGA198400.025.3452787
TATCAAC203500.025.0204092
GTACATG228200.022.1278421
TACATGG228450.021.5326162
ACATGGG226000.021.1888453
CGCAGAG237900.021.1193568
CATGGGG178650.018.631354
GCAGAGT279100.017.9113129
GAGTACT154550.017.84906412-13
CAGAGTA271650.016.63703510-11
AGAGTAC245150.016.45286610-11
GTACTTT170050.016.18559514-15
GTGGTAT60950.015.1887641
GAGTACA165300.014.6120941
Produced by FastQC (version 0.11.5)