##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140293_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8225971
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.188484739369976	33.0	31.0	34.0	30.0	34.0
2	32.257337522828614	33.0	31.0	34.0	30.0	34.0
3	32.873045261161266	34.0	31.0	34.0	30.0	37.0
4	35.955789170664474	37.0	35.0	37.0	35.0	37.0
5	35.86122999947362	37.0	35.0	37.0	35.0	37.0
6	35.90734248880771	37.0	35.0	37.0	35.0	37.0
7	35.91937948723622	37.0	35.0	37.0	35.0	37.0
8	36.19126106814624	37.0	35.0	37.0	35.0	39.0
9	37.584861167149754	39.0	37.0	39.0	35.0	39.0
10-11	37.49440528054378	39.0	37.0	39.0	35.0	39.0
12-13	37.62151933188191	39.0	37.0	39.0	34.5	39.5
14-15	38.74254285603487	40.0	38.0	41.0	34.5	41.0
16-17	38.60988021961177	40.0	38.0	41.0	34.0	41.0
18-19	38.67879019996545	40.0	38.0	41.0	34.0	41.0
20-21	38.69462945024574	40.0	38.0	41.0	34.0	41.0
22-23	38.62317103658979	40.0	38.0	41.0	34.0	41.0
24-25	38.55091805371892	40.0	38.0	41.0	34.0	41.0
26-27	38.356365668892735	40.0	38.0	41.0	34.0	41.0
28-29	38.2482880657364	40.0	38.0	41.0	34.0	41.0
30-31	38.122515156320745	40.0	38.0	41.0	33.0	41.0
32-33	37.96294051557396	40.0	37.0	41.0	33.0	41.0
34-35	38.04407697789085	40.0	37.0	41.0	33.0	41.0
36-37	38.01254131080309	40.0	37.0	41.0	33.0	41.0
38-39	37.96271281478324	40.0	37.0	41.0	33.0	41.0
40-41	37.907925049200415	40.0	37.0	41.0	33.0	41.0
42-43	37.96807812863595	40.0	37.0	41.0	33.0	41.0
44-45	37.96424775771635	40.0	37.0	41.0	33.0	41.0
46-47	37.898119261570066	40.0	37.0	41.0	33.0	41.0
48-49	37.804300490346286	40.0	37.0	41.0	33.0	41.0
50-51	37.67056450457356	40.0	37.0	41.0	32.0	41.0
52-53	37.500624061969624	39.5	36.0	41.0	32.0	41.0
54-55	37.35130737435364	39.0	36.0	41.0	31.5	41.0
56-57	37.204839709025634	39.0	35.5	41.0	31.0	41.0
58-59	37.03171088911258	39.0	35.0	41.0	31.0	41.0
60-61	36.83911962579127	39.0	35.0	40.0	31.0	41.0
62-63	36.615239745743516	38.0	35.0	40.0	31.0	41.0
64-65	36.31473684283869	38.0	35.0	40.0	30.5	41.0
66-67	36.01720506600451	37.0	34.5	40.0	30.0	41.0
68-69	35.70320647786454	37.0	34.0	39.5	30.0	41.0
70-71	35.400611384023875	36.0	34.0	39.0	29.0	41.0
72-73	35.1075479475952	36.0	34.0	39.0	29.0	40.0
74-75	34.64210329337419	35.0	33.5	38.0	29.0	40.0
76-77	33.66885926450793	34.5	32.0	36.5	28.0	39.0
78-79	34.25878844736721	35.0	33.5	37.0	29.0	39.0
80-81	34.14605201275633	35.0	33.5	36.5	29.0	39.0
82-83	33.91940938914933	35.0	33.0	36.0	29.0	37.5
84-85	33.68893636016618	35.0	33.0	36.0	29.0	37.0
86-87	33.49596688534043	35.0	33.0	35.0	29.0	37.0
88-89	33.338536884981366	35.0	33.0	35.0	29.0	36.0
90-91	33.21824244523958	35.0	33.0	35.0	29.0	36.0
92-93	33.16694017498026	35.0	33.0	35.0	29.0	36.0
94-95	33.11167275807988	35.0	33.0	35.0	29.0	36.0
96-97	33.15241535032534	35.0	33.0	35.0	29.0	35.0
98-99	33.14508542524622	34.5	32.5	35.0	29.0	35.0
100	32.03142694780583	34.0	31.0	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	11.0
20	48.0
21	295.0
22	1340.0
23	4595.0
24	12188.0
25	25842.0
26	46868.0
27	74791.0
28	106010.0
29	141469.0
30	179528.0
31	222539.0
32	277226.0
33	349257.0
34	462288.0
35	636751.0
36	981994.0
37	1649923.0
38	2004524.0
39	998269.0
40	50212.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.92293674788505	0.03178956402709954	23.622612287669416	30.422661400418434
2	15.159402594030317	23.918935215374148	37.47844021685482	23.44322197374071
3	17.12807115543704	23.962929442063423	32.74233263452726	26.166666767972274
4	13.34203585205929	22.68513954158117	36.92745816876816	27.04536643759138
5	17.46633923217089	36.566892832469264	32.14049745616657	13.826270479193278
6	28.940315982149706	36.76417045477063	19.952987920818103	14.342525642261563
7	26.81625062864919	32.58885789896414	22.12216648952446	18.472724982862204
8	24.657441656431807	32.05801479241782	21.795347929138092	21.489195622012282
9	23.693190749152894	20.382396193713788	23.679927877207444	32.24448517992587
10-11	25.17828594338589	25.78542399432237	28.32139208854493	20.714897973746808
12-13	25.02746636399924	23.699343439172313	28.42660328042752	22.846586916400927
14-15	22.374132108010173	26.330913597260185	26.219966141362605	25.074988153367034
16-17	21.86568541799138	28.633494271196803	27.283535777568396	22.217284533243422
18-19	21.870900623562626	27.921807428956807	28.73188265947621	21.47540928800436
20-21	22.61316747104382	27.2525569200324	28.54702485004472	21.58725075887906
22-23	22.419207033310787	27.046258477429934	28.346918336660128	22.187616152599148
24-25	22.1716163513534	27.26560894782009	28.593403464837046	21.969371235989463
26-27	22.19125931961906	27.516620346015657	28.37444112400183	21.917679210363445
28-29	22.120138533442876	27.392265970963788	28.390127044994877	22.097468450598456
30-31	22.279767605620066	27.365582995066934	28.375749517714365	21.978899881598633
32-33	22.150768567989026	27.534886397527465	28.285463362639053	22.02888167184446
34-35	22.29856596937853	27.331230980433098	28.285909701994722	22.084293348193654
36-37	22.259713110114728	27.416096151669215	28.316773633101842	22.00741710511421
38-39	22.08218430848416	27.63918805798306	28.342830880021296	21.935796753511486
40-41	22.390309438520948	27.31635523548432	28.13241857720862	22.16091674878611
42-43	22.27152640028656	27.417891768535778	28.143304920790392	22.167276910387265
44-45	22.29555945635719	27.73133011358101	27.82090301036116	22.15220741970064
46-47	22.36779403878818	27.455047606483518	27.627995651387877	22.54916270334042
48-49	22.25825303957726	27.633512908146518	27.78904786730773	22.319186184968494
50-51	22.22975364459938	27.80714945961666	27.577883529246595	22.385213366537364
52-53	22.4184905501195	27.651739767520873	27.500200289004773	22.429569393354853
54-55	22.3865240937572	27.60582837111589	27.58806103735122	22.419586497775686
56-57	22.404995581947897	27.89705709102518	27.372837896835573	22.32510943019135
58-59	22.407329946280274	27.755628620173127	27.495617353868713	22.341424079677882
60-61	22.45211209413617	27.767699466520483	27.510509367475155	22.269679071868186
62-63	22.34385707936216	28.01298061870387	27.39031925934197	22.252843042591998
64-65	22.37667587354899	27.761758983169983	27.371702581890396	22.489862561390634
66-67	22.375886163304447	27.771512699596663	27.33461601789707	22.51798511920182
68-69	22.33714762004157	27.850448006629648	27.44738608898672	22.365018284342067
70-71	22.36058742628559	27.472447837754725	27.486877109370667	22.680087626589014
72-73	22.36642477592506	27.608125450206934	27.50599433839971	22.519455435468295
74-75	22.257499042731293	27.814291549274913	27.497661286915047	22.430548121078747
76-77	22.513067058687653	27.577997379392848	27.246845917376707	22.662089644542792
78-79	22.257074408367732	27.64331170234114	27.463486171716166	22.636127717574965
80-81	22.33721128647437	27.712988909213358	27.442768600167128	22.507031204145143
82-83	22.439030596525093	27.517903233174152	27.51131952783719	22.531746642463567
84-85	22.506742154677177	27.559571002286425	27.51192771077789	22.421759132258508
86-87	22.36339880355846	27.65115200174358	27.49569144985862	22.489757744839345
88-89	22.48330252319115	27.445420958374367	27.48267614046227	22.58860037797221
90-91	22.464160984384744	27.525450423728504	27.53328964296829	22.47709894891846
92-93	22.40952443516015	27.538539267510576	27.59376015561919	22.458176141710087
94-95	22.36407812933053	27.496218972335896	27.636658352045885	22.503044546287683
96-97	22.316811502977654	27.776626384632912	27.814882005347208	22.09168010704222
98-99	23.42497483395419	29.90544402785596	27.290689658186707	19.378891480003148
100	22.638075886411528	30.316535936207472	21.383027865988478	25.662360311392522
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	832.0
1	810.0
2	1435.5
3	2212.0
4	3013.5
5	5108.5
6	6977.0
7	8197.5
8	9038.0
9	8894.0
10	8013.5
11	7002.5
12	7287.5
13	9336.0
14	15067.5
15	24158.5
16	32709.5
17	38374.0
18	40207.0
19	39031.0
20	36431.5
21	33687.0
22	32492.0
23	34157.5
24	39126.0
25	48092.0
26	60809.5
27	75567.5
28	90830.0
29	108858.5
30	128012.0
31	147319.0
32	167890.0
33	187477.5
34	207499.5
35	226120.5
36	244908.5
37	264424.0
38	280127.5
39	290262.0
40	300002.5
41	310874.0
42	319977.0
43	328760.5
44	337209.0
45	347432.5
46	356125.5
47	358731.5
48	356471.5
49	351513.0
50	345018.5
51	335522.0
52	322081.5
53	305976.0
54	289357.5
55	269558.5
56	247880.5
57	225164.5
58	200746.5
59	175749.5
60	147993.0
61	121242.0
62	97711.0
63	76637.0
64	58336.0
65	44343.5
66	34223.0
67	26462.5
68	20179.5
69	14801.0
70	10899.0
71	7944.0
72	5564.0
73	3876.0
74	2701.0
75	1938.0
76	1366.5
77	841.5
78	533.5
79	352.0
80	229.0
81	150.5
82	111.5
83	85.0
84	61.0
85	42.5
86	28.5
87	20.5
88	16.0
89	9.5
90	4.5
91	2.5
92	1.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.2156619565033722E-5
2	8.509633695523604E-5
3	4.37638304341214E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	6.078309782516861E-6
14-15	6.0783097825168604E-5
16-17	0.001525655755411732
18-19	0.003081703059736048
20-21	6.686422410845523E-5
22-23	0.0015866717626591936
24-25	0.00328956729238574
26-27	0.011782833318227137
28-29	0.007341061454180272
30-31	0.001682363442681268
32-33	0.00666448182432096
34-35	0.004245450246539361
36-37	0.002024036323392772
38-39	0.0021098568428102207
40-41	0.004601590668397546
42-43	0.00914231594928435
44-45	0.008697973429081839
46-47	0.009791201199233978
48-49	0.011172295590109583
50-51	0.032177224287127514
52-53	0.011286850733883982
54-55	0.0016679259617554466
56-57	0.007050824404340035
58-59	0.006230321281824152
60-61	3.456570931173776E-4
62-63	0.005254481087152136
64-65	0.0053678407891513515
66-67	0.0236519604249925
68-69	0.029725989345657632
70-71	0.015290833145529254
72-73	0.01024093329183073
74-75	0.015023595582026789
76-77	0.002715734278093994
78-79	0.0024196213367287693
80-81	0.0030405100311871615
82-83	0.022022586867764402
84-85	0.0015528862992956964
86-87	0.008408524543286423
88-89	0.0014390206600995627
90-91	0.0026265573736898315
92-93	0.0010354844791645613
94-95	0.0011174075332568416
96-97	0.0023192643258550623
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	201.0
20-21	711.0
22-23	1383.0
24-25	3099.0
26-27	4607.0
28-29	9392.0
30-31	16516.0
32-33	21526.0
34-35	31018.0
36-37	43574.0
38-39	51077.0
40-41	54432.0
42-43	61316.0
44-45	70539.0
46-47	70735.0
48-49	64803.0
50-51	60317.0
52-53	60138.0
54-55	66442.0
56-57	69097.0
58-59	70113.0
60-61	69433.0
62-63	69977.0
64-65	73607.0
66-67	79158.0
68-69	78964.0
70-71	77059.0
72-73	75107.0
74-75	77235.0
76-77	78576.0
78-79	83357.0
80-81	86132.0
82-83	85119.0
84-85	87486.0
86-87	94294.0
88-89	103220.0
90-91	110710.0
92-93	123694.0
94-95	160857.0
96-97	433202.0
98-99	1416504.0
100-101	3931244.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.54284996158864
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.51651896433528	71.36346375069319
2	8.145146896513493	13.283569825949982
3	2.3370523239369185	5.717097210095106
4	0.9155442778980255	2.9862435874331883
5	0.4166959792994105	1.6989288859804539
6	0.2391203971881148	1.1699135202399558
7	0.1311423726802125	0.7485605973348517
8	0.08915156952789427	0.5815738446282576
9	0.05202277556240165	0.3817876844043286
>10	0.15513552089299149	1.861222262107447
>50	0.0020878300941223485	0.10793961513128611
>100	3.2848944290412674E-4	0.057020002313315214
>500	2.6301314455284675E-5	0.01709313258320613
>1k	2.630131377071433E-5	0.025586081105394422
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	7.293971739020232E-5	0.0	1.215661956503372E-5	0.0
3	0.0	7.293971739020232E-5	0.0	4.862647826013488E-5	0.0
4	0.0	7.293971739020232E-5	0.0	4.862647826013488E-5	0.0
5	0.0	8.509633695523604E-5	0.0	4.862647826013488E-5	0.0
6	0.0	8.509633695523604E-5	0.0	4.862647826013488E-5	0.0
7	0.0	8.509633695523604E-5	0.0	4.862647826013488E-5	0.0
8	0.0	1.2156619565033721E-4	0.0	4.862647826013488E-5	0.0
9	0.0	3.1607210869087675E-4	0.0	7.293971739020232E-5	0.0
10-11	0.0	3.1607210869087675E-4	0.0	1.2764450543285407E-4	0.0
12-13	6.07830978251686E-6	3.1607210869087675E-4	0.0	1.5803605434543838E-4	0.0
14-15	1.215661956503372E-5	4.133250652111465E-4	0.0	1.7019267391047208E-4	0.0
16-17	2.431323913006744E-5	4.7410816303631513E-4	0.0	1.7627098369298895E-4	0.0
18-19	2.431323913006744E-5	4.7410816303631513E-4	0.0	2.3705408151815756E-4	0.0
20-21	2.431323913006744E-5	4.80186472818832E-4	0.0	2.6744563043074184E-4	0.0
22-23	3.0391548912584302E-5	4.984214021663825E-4	0.0	2.917588695608093E-4	0.0
24-25	4.862647826013488E-5	5.470478804265174E-4	0.0	3.525419673859779E-4	0.0
26-27	4.862647826013488E-5	5.895960489041355E-4	0.0	4.1940337499366334E-4	0.0
28-29	4.862647826013488E-5	5.956743586866523E-4	0.0	9.117464673775291E-4	0.0
30-31	4.862647826013488E-5	6.746923858593715E-4	0.0	0.0021030951847508337	0.0
32-33	4.862647826013488E-5	6.807706956418883E-4	0.0	0.004066389244503779	0.0
34-35	4.862647826013488E-5	7.293971739020232E-4	0.0	0.007044761037937042	0.0
36-37	4.862647826013488E-5	7.780236521621581E-4	0.0	0.011366439293306527	0.0
38-39	4.862647826013488E-5	8.023368912922255E-4	0.0	0.01773042963560168	0.0
40-41	4.862647826013488E-5	8.631199891173942E-4	0.0	0.024209907863764653	0.0
42-43	4.862647826013488E-5	0.0010333126630278663	0.0	0.031005458200618508	0.0
44-45	6.0783097825168604E-5	0.0011913487173733047	0.0	0.03855471895050444	0.0
46-47	1.8842760325802265E-4	0.0011913487173733047	0.0	0.04685769011342247	0.0
48-49	1.9450591304053953E-4	0.0011974270271558215	0.0	0.05485674578721467	0.0
50-51	1.9450591304053953E-4	0.0013615413912837766	0.0	0.06269776540666142	0.0
52-53	1.9450591304053953E-4	0.0013676197010662936	0.0	0.07062995967284591	0.0
54-55	1.9450591304053953E-4	0.0013980112499788779	0.0	0.07863509365642063	0.0
56-57	2.127408423880901E-4	0.0014223244891089454	0.0	0.08595945694435345	0.0
58-59	2.309757717356407E-4	0.0014284027988914622	0.0	0.09298598305294294	0.0
60-61	2.309757717356407E-4	0.0014648726575865632	0.0	0.0993560517050206	1.215661956503372E-5
62-63	2.309757717356407E-4	0.0014891858967166307	0.0	0.1058780781016612	1.215661956503372E-5
64-65	2.309757717356407E-4	0.0016776134999746534	0.0	0.1122238335146088	1.215661956503372E-5
66-67	2.3705408151815756E-4	0.0017140833586697546	0.0	0.11864860695472912	1.215661956503372E-5
68-69	2.735239402132587E-4	0.001744474907582339	0.0	0.12461142885137816	1.215661956503372E-5
70-71	2.796022499957756E-4	0.0017627098369298894	0.0	0.1301548473730335	1.215661956503372E-5
72-73	2.796022499957756E-4	0.00178094476627744	0.0	0.13433064619362262	1.215661956503372E-5
74-75	2.978371793433261E-4	0.0018113363151900244	0.0	0.13770410812291947	1.215661956503372E-5
76-77	3.03915489125843E-4	0.001866041103232676	0.0	0.14128423258482192	1.215661956503372E-5
78-79	3.099937989083599E-4	0.0018964326521452605	0.0	0.14328399650326995	1.215661956503372E-5
80-81	3.2215041847339363E-4	0.0018964326521452605	0.0	0.1439526105793468	1.215661956503372E-5
82-83	3.2822872825591045E-4	0.0018964326521452605	0.0	0.1445239716989034	1.215661956503372E-5
84-85	3.2822872825591045E-4	0.0018964326521452605	0.0	0.14473671254129147	1.215661956503372E-5
86-87	3.646985869510116E-4	0.001908589271710294	0.0	0.14486435704672435	1.215661956503372E-5
88	3.646985869510116E-4	0.0019450591304053953	0.0	0.14487043535650684	1.215661956503372E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10450	0.0	40.912014	1
GTATCAA	16925	0.0	30.879225	1
ATCAACG	18085	0.0	27.804996	3
TCAACGC	19020	0.0	26.437975	4
CAACGCA	18950	0.0	26.424608	5
AACGCAG	19380	0.0	25.903444	6
ACGCAGA	19840	0.0	25.345278	7
TATCAAC	20350	0.0	25.020409	2
GTACATG	22820	0.0	22.127842	1
TACATGG	22845	0.0	21.532616	2
ACATGGG	22600	0.0	21.188845	3
CGCAGAG	23790	0.0	21.119356	8
CATGGGG	17865	0.0	18.63135	4
GCAGAGT	27910	0.0	17.911312	9
GAGTACT	15455	0.0	17.849064	12-13
CAGAGTA	27165	0.0	16.637035	10-11
AGAGTAC	24515	0.0	16.452866	10-11
GTACTTT	17005	0.0	16.185595	14-15
GTGGTAT	6095	0.0	15.188764	1
GAGTACA	16530	0.0	14.612094	1
>>END_MODULE