FastQCFastQC Report
Fri 27 May 2016
SRR2140292_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140292_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7829491
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98600.037.888591
GTATCAA161500.029.9723661
ATCAACG176550.026.342653
TCAACGC176900.026.3143654
CAACGCA179250.025.969385
AACGCAG186100.025.0588076
TATCAAC193050.024.527972
GTACATG212400.021.9956551
TACATGG206850.021.7500652
ACGCAGA218850.021.3473957
ACATGGG209600.021.1025943
CGCAGAG222350.020.9734388
CATGGGG163300.018.4875134
TATACCG6750.018.1153145
GCAGAGT264550.017.4525159
GTGGTAT65700.017.3280641
GAGTACT146750.017.01230612-13
AGAGTAC225700.016.58020410-11
CAGAGTA249400.016.37405410-11
GAGTACA149950.015.7468331
Produced by FastQC (version 0.11.5)