FastQCFastQC Report
Fri 27 May 2016
SRR2140291_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140291_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7265075
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81700.041.2828751
GTATCAA140600.033.4307441
ATCAACG159150.028.7260783
TCAACGC159750.028.618194
CAACGCA162500.028.0572915
AACGCAG169100.027.1094136
TATCAAC171850.026.989432
ACGCAGA199600.022.9045877
CGCAGAG205300.022.2484518
GTACATG196650.021.1596471
TACATGG194850.021.1209132
ACATGGG198550.020.3303283
GCAGAGT241700.018.8120169
GTGGTAT48200.018.6773011
CATGGGG155900.018.4411744
GAGTACT135550.018.13529612-13
AGAGTAC215450.016.81991610-11
CAGAGTA236050.016.54716910-11
ATGGGGG102600.016.133445
GGACCGA11150.015.9990816
Produced by FastQC (version 0.11.5)