##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140291_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7265075
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.049142369486894	33.0	31.0	34.0	30.0	34.0
2	32.182722408233914	33.0	31.0	34.0	30.0	34.0
3	32.08476512630634	33.0	31.0	34.0	30.0	34.0
4	35.973732824506286	37.0	35.0	37.0	35.0	37.0
5	35.84385873511285	37.0	35.0	37.0	35.0	37.0
6	35.898315571415296	37.0	35.0	37.0	35.0	37.0
7	35.848939618655	37.0	35.0	37.0	35.0	37.0
8	35.841640038127615	37.0	35.0	37.0	35.0	37.0
9	37.59417789905817	39.0	37.0	39.0	35.0	39.0
10-11	37.47485098226791	39.0	37.0	39.0	34.5	39.0
12-13	37.45083897963889	39.0	37.0	39.0	34.0	39.0
14-15	38.74469644153708	40.0	38.0	41.0	34.0	41.0
16-17	38.59381802665492	40.0	38.0	41.0	34.0	41.0
18-19	38.661716981586565	40.0	38.0	41.0	34.0	41.0
20-21	38.69274446715782	40.0	38.0	41.0	34.0	41.0
22-23	38.62121419853697	40.0	38.0	41.0	34.0	41.0
24-25	38.569529686777486	40.0	38.0	41.0	34.0	41.0
26-27	38.36460661025715	40.0	38.0	41.0	34.0	41.0
28-29	38.25819812917702	40.0	38.0	41.0	34.0	41.0
30-31	38.13836575248281	40.0	38.0	41.0	33.0	41.0
32-33	37.96083749206679	40.0	37.0	41.0	33.0	41.0
34-35	38.043924368353885	40.0	37.0	41.0	33.0	41.0
36-37	38.02056901376585	40.0	37.0	41.0	33.0	41.0
38-39	37.971879036992206	40.0	37.0	41.0	33.0	41.0
40-41	37.90575523670093	40.0	37.0	41.0	33.0	41.0
42-43	37.97404213860594	40.0	37.0	41.0	33.0	41.0
44-45	37.98437898524912	40.0	37.0	41.0	33.0	41.0
46-47	37.924452449381235	40.0	37.0	41.0	33.0	41.0
48-49	37.83794571978346	40.0	37.0	41.0	33.0	41.0
50-51	37.717625902902896	40.0	37.0	41.0	32.5	41.0
52-53	37.549037453372165	40.0	36.0	41.0	32.0	41.0
54-55	37.40558331007945	39.0	36.0	41.0	32.0	41.0
56-57	37.271657557005156	39.0	36.0	41.0	31.0	41.0
58-59	37.10831972181307	39.0	35.0	41.0	31.0	41.0
60-61	36.92403800832291	39.0	35.0	40.5	31.0	41.0
62-63	36.715440885317676	38.0	35.0	40.0	31.0	41.0
64-65	36.41959239884186	38.0	35.0	40.0	30.5	41.0
66-67	36.12324704108104	37.5	34.5	40.0	30.0	41.0
68-69	35.81735632264216	37.0	34.0	39.5	30.0	41.0
70-71	35.51143270961633	36.0	34.0	39.0	30.0	41.0
72-73	35.22785707622388	36.0	34.0	39.0	29.0	40.5
74-75	34.89994817627983	35.5	34.0	38.5	29.0	40.0
76-77	33.62086315534946	34.5	32.0	36.5	28.0	39.0
78-79	34.31845561700672	35.0	33.5	37.0	29.0	39.0
80-81	34.23119101573424	35.0	34.0	37.0	29.0	39.0
82-83	34.003924607351145	35.0	33.5	36.0	29.0	38.5
84-85	33.7636850708374	35.0	33.0	36.0	29.0	37.0
86-87	33.56558521851815	35.0	33.0	35.5	29.0	37.0
88-89	33.396198920207226	35.0	33.0	35.0	29.0	36.5
90-91	33.267026745834556	35.0	33.0	35.0	29.0	36.0
92-93	33.21452535117755	35.0	33.0	35.0	29.0	36.0
94-95	33.146172193219556	35.0	33.0	35.0	29.0	36.0
96-97	33.18087180247505	35.0	33.0	35.0	29.0	35.0
98-99	33.29076146282602	35.0	33.0	35.0	29.0	35.0
100	32.09840918291557	34.0	31.0	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	6.0
20	63.0
21	258.0
22	1158.0
23	4128.0
24	10507.0
25	22454.0
26	40418.0
27	64459.0
28	91770.0
29	122901.0
30	156257.0
31	195110.0
32	240015.0
33	303988.0
34	404839.0
35	554365.0
36	849293.0
37	1429057.0
38	1778598.0
39	956318.0
40	39111.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.3643217723148	20.533800408116914	13.945761055460542	24.156116764107736
2	14.912619621958479	21.28107418023902	40.62995908507483	23.176347112727676
3	18.173688358716475	25.876035676521784	30.674469494944155	25.275806469817585
4	12.646269997212691	17.818590998716463	38.637316201140386	30.89782280293046
5	13.061337976552204	37.95823167689253	35.867737084613715	13.11269326194155
6	30.417043182623715	37.93279491264715	18.417139534003436	13.233022370725697
7	26.842448288558618	32.55904446960286	23.008599360639774	17.589907881198748
8	24.8010516064872	36.69539543638572	20.987615406585615	17.51593755054146
9	25.468367497926724	16.628651459207237	21.306111774482712	36.59686926838332
10-11	24.386369032666558	26.7540046042195	28.839192988372453	20.020433374741486
12-13	24.91360378247988	24.158319081358417	29.10180555603349	21.826271580128214
14-15	21.993847276180905	25.697319022859368	26.525851419290237	25.782982281669494
16-17	21.75663875290193	28.937401737702746	27.200634990200957	22.105324519194372
18-19	21.73585346659874	28.196051068707867	28.88307608150588	21.18501938318751
20-21	22.472279755980086	27.353565822786724	28.704246782816416	21.469907638416768
22-23	22.294859244479216	27.31400214948991	28.521957932827817	21.869180673203058
24-25	22.109815337250605	27.424532928291264	28.606736385510384	21.85891534894775
26-27	22.107223561199497	27.54170936407565	28.516801539503934	21.834265535220922
28-29	21.94271789075406	27.6980385458597	28.488459857126962	21.87078370625928
30-31	22.15738813496857	27.58097570871973	28.465792914766563	21.79584324154514
32-33	22.0458465537221	27.571392867838608	28.43650340970273	21.94625716873656
34-35	22.11159401428468	27.58091575794185	28.49034430544099	21.817145922332486
36-37	22.153843963478018	27.604245981391166	28.37074813172603	21.87116192340478
38-39	22.062245021326127	27.70339249916354	28.38773346979056	21.846629009719763
40-41	22.179255975383402	27.545119519004768	28.30456777117696	21.971056734434868
42-43	22.14326424577899	27.682714826537296	28.129178178360338	22.04484274932338
44-45	22.199678509069752	27.795394966167226	27.968866384636055	22.036060140126967
46-47	22.190693521762007	27.733826886121214	27.743797403753117	22.33168218836366
48-49	22.217889047474067	27.887491958619748	27.67698083602019	22.217638157886
50-51	22.11976934605869	27.854940567364434	27.778999467055144	22.246290619521737
52-53	22.242544342507646	27.870547165372855	27.635278287461773	22.251630204657726
54-55	22.26382587669426	27.767005614136615	27.660876255981275	22.308292253187854
56-57	22.376883998005837	27.850095841910733	27.52347700216895	22.249543157914477
58-59	22.165097145666145	28.04741762239819	27.64552883370555	22.141956398230107
60-61	22.37372352822926	28.01356852807163	27.508431979958065	22.104275963741053
62-63	22.287382941469282	28.116277034063774	27.410222893705722	22.186117130761218
64-65	22.261348722303715	28.017761329421752	27.44395603335135	22.276933914923184
66-67	22.35452821417203	27.981556908989152	27.343408436113208	22.32050644072561
68-69	22.225113835625006	27.933747097090517	27.48054924853887	22.36058981874561
70-71	22.211091857836315	27.778006121505484	27.55080132775751	22.460100692900685
72-73	22.307975130416118	27.827010423731746	27.50635894070912	22.35865550514302
74-75	22.235829093923638	27.95281318433081	27.42989485373384	22.381462868011717
76-77	22.295123333331887	27.89340261082614	27.383828035272906	22.427646020569068
78-79	22.201574886717378	27.87925341358998	27.414648829149357	22.504522870543287
80-81	22.206981099463473	27.893588928031132	27.481864786413425	22.41756518609197
82-83	22.197853274811703	27.910762949429458	27.615919074404644	22.275464701354196
84-85	22.38225861968607	27.860330537982442	27.480916310430153	22.276494531901328
86-87	22.181995314179222	27.913966886668035	27.52183826178079	22.382199537371953
88-89	22.30333420801689	27.817290830266266	27.462571357919757	22.416803603797085
90-91	22.269738562996608	27.881446297014346	27.52203004117506	22.326785098813986
92-93	22.278805418384913	27.872731515086123	27.561180948337892	22.287282118191065
94-95	22.069762824260618	27.988026915209442	27.609527401788736	22.332682858741208
96-97	22.081446200315575	28.125822248360038	27.73233903315553	22.060392518168854
98-99	23.27949085602463	30.067322437283938	27.81882209226565	18.834364614425784
100	22.266769935636603	31.07044160000154	21.481638549298818	25.18114991506304
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1245.0
1	1148.0
2	1614.0
3	2333.0
4	3145.0
5	5175.5
6	6981.5
7	8071.5
8	8734.0
9	8588.0
10	7812.0
11	6903.0
12	7327.5
13	9685.5
14	15125.0
15	22798.5
16	29647.0
17	34314.0
18	36284.0
19	35952.0
20	34327.0
21	32501.0
22	32360.5
23	35055.5
24	41002.5
25	50319.0
26	62522.0
27	76515.0
28	91128.5
29	107447.5
30	124497.5
31	141883.0
32	160261.5
33	178079.0
34	195604.5
35	212151.0
36	228718.0
37	245205.5
38	257106.0
39	262954.5
40	270901.0
41	281098.5
42	288970.0
43	297027.0
44	305627.0
45	312889.0
46	317572.0
47	320422.0
48	318111.5
49	312921.5
50	305840.5
51	294506.0
52	281352.5
53	267531.5
54	253204.5
55	235568.0
56	215508.5
57	194368.5
58	172850.5
59	150582.0
60	126575.0
61	104477.0
62	85121.5
63	67389.0
64	51696.5
65	39703.5
66	30888.5
67	24026.0
68	18162.0
69	13244.5
70	9711.0
71	7067.5
72	5052.0
73	3511.5
74	2402.5
75	1615.5
76	1061.5
77	662.5
78	435.5
79	301.5
80	209.5
81	144.5
82	106.0
83	68.0
84	43.5
85	32.5
86	25.0
87	18.0
88	11.0
89	5.5
90	2.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	2.3399620788498398E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.001114923108157865
18-19	0.0037714682917932714
20-21	0.0
22-23	0.0013146764908104458
24-25	5.576595154062736E-4
26-27	0.013844029717415116
28-29	0.007547143282205056
30-31	7.247964460952316E-4
32-33	0.006851534920301078
34-35	0.00464578461711964
36-37	0.002135563326710761
38-39	0.001813461195936891
40-41	0.0036772200046719826
42-43	0.008533423289241598
44-45	0.010075803095999813
46-47	0.009093803237247984
48-49	0.008551651940830242
50-51	0.03513948863769778
52-53	0.012832980081408513
54-55	6.842145915907899E-5
56-57	0.008223787867468817
58-59	0.006536814390381044
60-61	1.0220333085372331E-4
62-63	0.005183472260195043
64-65	0.00241251031348159
66-67	0.02524530523617427
68-69	0.032588078931097816
70-71	0.01592861441518723
72-73	0.01127325161001146
74-75	0.017037798141133414
76-77	0.0024105489088667707
78-79	0.0027849845231293843
80-81	0.001755133565218848
82-83	0.024680234620377595
84-85	0.0010083144694501339
86-87	0.010120824787685934
88-89	8.790870576927822E-4
90-91	0.002827243316598745
92-93	0.0010877496458933922
94-95	6.808759469056942E-4
96-97	0.0031960983680721735
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	156.0
20-21	539.0
22-23	1205.0
24-25	2837.0
26-27	4144.0
28-29	8650.0
30-31	17751.0
32-33	22692.0
34-35	31747.0
36-37	47685.0
38-39	56637.0
40-41	59429.0
42-43	65734.0
44-45	75766.0
46-47	75658.0
48-49	70524.0
50-51	64954.0
52-53	64726.0
54-55	70545.0
56-57	72706.0
58-59	72950.0
60-61	71371.0
62-63	70838.0
64-65	74421.0
66-67	78427.0
68-69	77902.0
70-71	75006.0
72-73	72021.0
74-75	73583.0
76-77	73746.0
78-79	78439.0
80-81	80038.0
82-83	78143.0
84-85	78516.0
86-87	84059.0
88-89	88874.0
90-91	94998.0
92-93	104874.0
94-95	125985.0
96-97	274236.0
98-99	467717.0
100-101	4154846.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.48106270323613
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.35687588630874	75.48963834716538
2	7.290109698274318	12.31752429066765
3	1.851663146428137	4.692914111360008
4	0.676131050999346	2.284810788603228
5	0.3266829518503392	1.379926146967339
6	0.16497085369060432	0.8362147820905403
7	0.10100595158008664	0.5973163090186133
8	0.05920700085545204	0.4001496281391999
9	0.0422281624283513	0.32107320341567874
>10	0.13011164289294747	1.606280821786215
>50	8.800968590832052E-4	0.04713182107923643
>100	1.3355783263109785E-4	0.027019749706934805
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.3764482816763763E-5	0.0	0.0	0.0
3	0.0	1.3764482816763763E-5	0.0	1.3764482816763763E-5	0.0
4	0.0	8.258689690058258E-5	0.0	5.505793126705505E-5	0.0
5	0.0	8.258689690058258E-5	0.0	5.505793126705505E-5	0.0
6	6.882241408381881E-5	8.258689690058258E-5	0.0	5.505793126705505E-5	0.0
7	6.882241408381881E-5	8.258689690058258E-5	0.0	5.505793126705505E-5	0.0
8	6.882241408381881E-5	9.635137971734635E-5	0.0	6.882241408381881E-5	0.0
9	6.882241408381881E-5	1.2388034535087386E-4	0.0	8.258689690058258E-5	0.0
10-11	6.882241408381881E-5	1.2388034535087386E-4	0.0	1.101158625341101E-4	0.0
12-13	6.882241408381881E-5	1.2388034535087386E-4	0.0	1.3076258675925575E-4	0.0
14-15	6.882241408381881E-5	1.7893827661792893E-4	0.0	1.6517379380116517E-4	0.0
16-17	1.927027594346927E-4	1.927027594346927E-4	0.0	1.7893827661792893E-4	0.0
18-19	2.0646724225145644E-4	2.1334948365983833E-4	0.0	1.927027594346927E-4	0.0
20-21	2.0646724225145644E-4	2.3399620788498398E-4	0.0	2.477606907017477E-4	0.0
22-23	2.0646724225145644E-4	2.3399620788498398E-4	0.0	3.5099431182747593E-4	0.0
24-25	2.0646724225145644E-4	2.4087844929336584E-4	0.0	4.3358120872805854E-4	0.0
26-27	2.0646724225145644E-4	2.477606907017477E-4	0.0	5.092858642202592E-4	0.0
28-29	2.0646724225145644E-4	2.477606907017477E-4	0.0	8.74044658864499E-4	0.0
30-31	2.0646724225145644E-4	3.3034758760233033E-4	0.0	0.002202317250682202	0.0
32-33	2.271139664766021E-4	3.441120704190941E-4	0.0	0.004604219502207478	0.0
34-35	2.4087844929336584E-4	3.854055188693854E-4	0.0	0.007845755205555345	0.0
36-37	2.477606907017477E-4	4.2669896731967666E-4	0.0	0.01301431850325014	0.0
38-39	2.546429321101296E-4	4.404634501364404E-4	0.0	0.02077060457049652	0.0
40-41	2.615251735185115E-4	4.6799241576996796E-4	0.0	0.029634931504492385	0.0
42-43	2.615251735185115E-4	4.886391399951136E-4	0.0	0.038210204299336206	0.0
44-45	2.615251735185115E-4	5.712260368956961E-4	0.0	0.04809998520318097	0.0
46-47	2.615251735185115E-4	6.331662095711331E-4	0.0	0.059194158353492565	0.0
48-49	2.615251735185115E-4	6.331662095711331E-4	0.0	0.0695863428801492	0.0
50-51	2.8217189774365714E-4	6.882241408381882E-4	0.0	0.07886360429864799	0.0
52-53	3.028186219688028E-4	6.882241408381882E-4	0.0	0.08890479451347715	0.0
54-55	3.028186219688028E-4	6.882241408381882E-4	0.0	0.09870510627901294	1.3764482816763763E-5
56-57	3.028186219688028E-4	6.882241408381882E-4	0.0	0.10713585200428075	1.3764482816763763E-5
58-59	3.028186219688028E-4	6.951063822465701E-4	0.0	0.11564230238504075	1.3764482816763763E-5
60-61	3.3034758760233033E-4	7.01988623654952E-4	0.0	0.1239078743165074	1.3764482816763763E-5
62-63	3.854055188693854E-4	7.01988623654952E-4	0.0	0.1322216219378327	1.3764482816763763E-5
64-65	3.991700016861491E-4	7.708110377387708E-4	0.0	0.14045966490366585	1.3764482816763763E-5
66-67	4.129344845029129E-4	7.708110377387708E-4	0.0	0.14822971545372898	1.3764482816763763E-5
68-69	4.129344845029129E-4	7.708110377387708E-4	0.0	0.15588276789984962	1.3764482816763763E-5
70-71	4.3358120872805854E-4	7.708110377387708E-4	0.0	0.1622350767197861	1.3764482816763763E-5
72-73	4.6799241576996796E-4	7.708110377387708E-4	0.0	0.16720405501663782	1.3764482816763763E-5
74-75	4.8175689858673173E-4	7.845755205555346E-4	0.0	0.17146416244842622	1.3764482816763763E-5
76-77	4.8175689858673173E-4	8.18986727597444E-4	0.0	0.1757655633286649	1.3764482816763763E-5
78-79	4.8175689858673173E-4	8.396334518225895E-4	0.0	0.17849781316779248	1.3764482816763763E-5
80-81	4.8175689858673173E-4	8.396334518225895E-4	0.0	0.17953703162045814	1.3764482816763763E-5
82-83	4.8175689858673173E-4	8.396334518225895E-4	0.0	0.18018396231284606	1.3764482816763763E-5
84-85	4.886391399951136E-4	8.396334518225895E-4	0.0	0.1803560183480556	1.3764482816763763E-5
86-87	4.955213814034954E-4	8.396334518225895E-4	0.0	0.1805005454176316	1.3764482816763763E-5
88	5.368148298537867E-4	8.533979346393533E-4	0.0	0.18050742765904	1.3764482816763763E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8170	0.0	41.282875	1
GTATCAA	14060	0.0	33.430744	1
ATCAACG	15915	0.0	28.726078	3
TCAACGC	15975	0.0	28.61819	4
CAACGCA	16250	0.0	28.057291	5
AACGCAG	16910	0.0	27.109413	6
TATCAAC	17185	0.0	26.98943	2
ACGCAGA	19960	0.0	22.904587	7
CGCAGAG	20530	0.0	22.248451	8
GTACATG	19665	0.0	21.159647	1
TACATGG	19485	0.0	21.120913	2
ACATGGG	19855	0.0	20.330328	3
GCAGAGT	24170	0.0	18.812016	9
GTGGTAT	4820	0.0	18.677301	1
CATGGGG	15590	0.0	18.441174	4
GAGTACT	13555	0.0	18.135296	12-13
AGAGTAC	21545	0.0	16.819916	10-11
CAGAGTA	23605	0.0	16.547169	10-11
ATGGGGG	10260	0.0	16.13344	5
GGACCGA	1115	0.0	15.999081	6
>>END_MODULE