FastQCFastQC Report
Fri 27 May 2016
SRR2140290_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140290_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9543223
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA103050.045.4383351
GTATCAA178450.033.6660841
ATCAACG203050.028.743183
TCAACGC203350.028.7004744
CAACGCA206850.028.214855
AACGCAG216300.027.0208196
TATCAAC224200.026.4046272
ACGCAGA251750.023.166087
CGCAGAG258700.022.5275638
GTACATG257450.021.4517291
TACATGG262500.020.7682882
ACATGGG265700.019.8887713
GCAGAGT307450.018.7107759
CATGGGG204050.018.234814
GAGTACT167400.018.13329912-13
CAGAGTA302100.017.00109510-11
AGAGTAC270550.016.96708310-11
GTACTTT191850.015.844830514-15
GTGGTAT61900.015.6017661
GAGTACA190100.015.3506131
Produced by FastQC (version 0.11.5)