##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140290_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9543223
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.049586077994825	33.0	31.0	34.0	30.0	34.0
2	32.18437418888776	33.0	31.0	34.0	30.0	34.0
3	32.088253936851316	33.0	31.0	34.0	30.0	34.0
4	35.975768354150375	37.0	35.0	37.0	35.0	37.0
5	35.84508839414106	37.0	35.0	37.0	35.0	37.0
6	35.89852788727666	37.0	35.0	37.0	35.0	37.0
7	35.84977266066192	37.0	35.0	37.0	35.0	37.0
8	35.84008369080341	37.0	35.0	37.0	35.0	37.0
9	37.59495822323339	39.0	37.0	39.0	35.0	39.0
10-11	37.47395513025317	39.0	37.0	39.0	34.5	39.0
12-13	37.452881641768194	39.0	37.0	39.0	34.0	39.0
14-15	38.749890995945506	40.0	38.0	41.0	34.0	41.0
16-17	38.598531072783274	40.0	38.0	41.0	34.0	41.0
18-19	38.66320309186949	40.0	38.0	41.0	34.0	41.0
20-21	38.692321355043454	40.0	38.0	41.0	34.0	41.0
22-23	38.62277643486382	40.0	38.0	41.0	34.0	41.0
24-25	38.57308433423523	40.0	38.0	41.0	34.0	41.0
26-27	38.37115151823389	40.0	38.0	41.0	34.0	41.0
28-29	38.26522186535121	40.0	38.0	41.0	34.0	41.0
30-31	38.1460170300102	40.0	38.0	41.0	33.5	41.0
32-33	37.968028471853174	40.0	37.0	41.0	33.0	41.0
34-35	38.05601969448884	40.0	37.0	41.0	33.0	41.0
36-37	38.03298971489463	40.0	37.0	41.0	33.0	41.0
38-39	37.990126068483946	40.0	37.0	41.0	33.0	41.0
40-41	37.92275331219287	40.0	37.0	41.0	33.0	41.0
42-43	37.983106918704024	40.0	37.0	41.0	33.0	41.0
44-45	37.989845314807454	40.0	37.0	41.0	33.0	41.0
46-47	37.93094789468301	40.0	37.0	41.0	33.0	41.0
48-49	37.84397274539752	40.0	37.0	41.0	33.0	41.0
50-51	37.727217432252246	40.0	37.0	41.0	32.5	41.0
52-53	37.55226668100015	40.0	36.0	41.0	32.0	41.0
54-55	37.408267900639316	39.0	36.0	41.0	32.0	41.0
56-57	37.27464112628567	39.0	36.0	41.0	31.0	41.0
58-59	37.110972218865676	39.0	35.0	41.0	31.0	41.0
60-61	36.92413652035101	39.0	35.0	40.5	31.0	41.0
62-63	36.713942742018645	38.0	35.0	40.0	31.0	41.0
64-65	36.414961325124374	38.0	35.0	40.0	30.5	41.0
66-67	36.12450394124484	37.5	34.5	40.0	30.0	41.0
68-69	35.81183149276153	37.0	34.0	39.5	30.0	41.0
70-71	35.50425121803187	36.0	34.0	39.0	30.0	41.0
72-73	35.21577510833232	36.0	34.0	39.0	29.0	40.5
74-75	34.886785995831346	35.5	34.0	38.5	29.0	40.0
76-77	33.61030900216201	34.5	32.0	36.5	28.0	39.0
78-79	34.30509686906569	35.0	33.5	37.0	29.0	39.0
80-81	34.217146379716496	35.0	34.0	37.0	29.0	39.0
82-83	33.98807827914723	35.0	33.5	36.0	29.0	38.5
84-85	33.74849958887326	35.0	33.0	36.0	29.0	37.0
86-87	33.5474040948251	35.0	33.0	35.5	29.0	37.0
88-89	33.38125176492248	35.0	33.0	35.0	29.0	36.5
90-91	33.25572695024676	35.0	33.0	35.0	29.0	36.0
92-93	33.20308465863805	35.0	33.0	35.0	29.0	36.0
94-95	33.13188964988653	35.0	33.0	35.0	29.0	36.0
96-97	33.16715447583092	35.0	33.0	35.0	29.0	35.0
98-99	33.28242441479296	35.0	33.0	35.0	29.0	35.0
100	32.08582827534231	34.0	31.0	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	4.0
19	17.0
20	52.0
21	329.0
22	1493.0
23	5277.0
24	13668.0
25	29641.0
26	53010.0
27	84618.0
28	122221.0
29	163141.0
30	207505.0
31	257274.0
32	317878.0
33	401218.0
34	532879.0
35	730094.0
36	1116823.0
37	1888546.0
38	2347213.0
39	1220403.0
40	49918.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.48740944228171	20.49551812841427	13.929665061793065	24.087407367510956
2	14.986970334864857	21.22062955041499	40.72835770472931	23.06404240999084
3	18.32133139659288	25.795536541166246	30.526243762300666	25.356888299940213
4	12.649688684839495	17.675254995089183	38.68005599366168	30.995000326409645
5	13.126362026749245	37.924608908332125	35.821744917833314	13.12728414708532
6	30.56498836923333	37.83286841353283	18.443339320479048	13.158803896754797
7	26.91068834920865	32.52534285324779	22.981806041837228	17.582162755706328
8	24.706862660549795	36.86137272491694	20.981831819292076	17.44993279524119
9	25.45566628800354	16.68086347767416	21.25964152781508	36.60382870650722
10-11	24.49696501905069	26.725232135935627	28.791667133839375	19.986135711174306
12-13	24.97404702792757	24.102941951581766	29.114828397072976	21.808182623417686
14-15	22.0012620474236	25.72730931677904	26.496504378028263	25.7749242577691
16-17	21.83014787443177	28.878463938012793	27.228858496695608	22.06252969085983
18-19	21.732263249103713	28.22208221065744	28.82611480569902	21.21953973453982
20-21	22.48736890143106	27.393249337335607	28.7156702118797	21.40371154935363
22-23	22.26984819082773	27.310748388706646	28.54062847659018	21.878774943875445
24-25	22.07975939554556	27.40626073242623	28.620304664247907	21.893675207780298
26-27	22.067342197889133	27.61249602026216	28.477105863333602	21.843055918515102
28-29	21.962070211401834	27.7502249706304	28.47449583521694	21.813208982750826
30-31	22.199765565530612	27.56817939720633	28.43145628179536	21.800598755467703
32-33	22.039116316563966	27.619589944244012	28.36471754003397	21.976576199158057
34-35	22.09222366638339	27.701759431070776	28.421727065998848	21.78428983654699
36-37	22.140837247711733	27.615398391806277	28.363673013263202	21.880091347218787
38-39	21.991423208076068	27.732548240454	28.41782960523031	21.858198946239614
40-41	22.24082867916769	27.603124703063454	28.274702851283756	21.8813437664851
42-43	22.14446960416101	27.674800486911966	28.100799741214345	22.07993016771268
44-45	22.209842528327766	27.82592042505327	27.924843298850398	22.03939374776857
46-47	22.212578282329005	27.8011127665377	27.731910236354658	22.254398714778638
48-49	22.17562654671524	27.865572784873216	27.769880960774206	22.188919707637336
50-51	22.153864559514073	27.928023147929775	27.7045979732687	22.213514319287455
52-53	22.26893675001507	27.877424662900356	27.577534179491188	22.276104407593383
54-55	22.267000737607766	27.80100427254092	27.63089448272595	22.301100507125366
56-57	22.329828121939055	27.885061350349126	27.59234335623076	22.19276717148106
58-59	22.217961888797124	27.994133234775415	27.632049657309793	22.15585521911767
60-61	22.31750703220069	27.927450367512797	27.62142471046708	22.133617889819433
62-63	22.27971240283777	28.022140853645123	27.494326148412064	22.203820595105046
64-65	22.224920671222552	28.01430677360638	27.508758277798574	22.252014277372496
66-67	22.2710064116102	27.97405124838826	27.42842288529468	22.32651945470686
68-69	22.231272794565545	27.955555898250456	27.52446353255045	22.28870777463355
70-71	22.191461180752466	27.782822191823254	27.57727020949547	22.448446417928814
72-73	22.290287726357004	27.82382853077633	27.53488479049857	22.350998952368094
74-75	22.141330752580274	27.96379683192871	27.525443445463882	22.36942897002713
76-77	22.23757168854354	27.91059078232192	27.451193938695063	22.400643590439483
78-79	22.184291661360096	27.859968913705547	27.487347636944122	22.46839178799024
80-81	22.184497530563213	27.93685295935388	27.511925239725777	22.366724270357132
82-83	22.237849569230395	27.90780687402451	27.61307740065263	22.241266156092465
84-85	22.348434775557237	27.857870965198796	27.51854948109821	22.27514477814575
86-87	22.21140756308346	27.871271206882685	27.586719826546485	22.330601403487368
88-89	22.235449997145025	27.84169768121854	27.583327765370235	22.339524556266195
90-91	22.266565811481833	27.90808407403187	27.54165916710816	22.283690947378133
92-93	22.288972652067372	27.869589619372466	27.614668394796084	22.226769333764075
94-95	22.127612724046145	27.974155702225996	27.646208299637255	22.252023274090604
96-97	22.099231470896473	28.095385563533643	27.738432249748357	22.066950715821523
98-99	23.228445702743528	30.020133927830205	27.930188561410674	18.821231808015593
100	22.209642339619194	31.05102559300147	21.61436914537352	25.12496292200582
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1523.0
1	1375.5
2	1901.0
3	2700.5
4	3573.0
5	5904.5
6	8007.0
7	9312.0
8	10028.5
9	9740.5
10	8816.5
11	7907.5
12	8605.5
13	11611.5
14	18601.0
15	28592.5
16	37205.5
17	42764.0
18	45043.0
19	44440.0
20	42250.0
21	39849.5
22	39228.5
23	41997.5
24	48630.0
25	59971.5
26	75678.5
27	93747.5
28	113120.5
29	135363.0
30	158788.5
31	182224.5
32	207165.0
33	230664.5
34	253744.0
35	275759.5
36	298160.5
37	319997.5
38	336476.0
39	349557.0
40	362289.0
41	374411.5
42	383882.0
43	393614.5
44	402444.5
45	411678.0
46	418276.0
47	419965.0
48	418744.5
49	410794.5
50	400861.5
51	389557.5
52	371379.0
53	348546.5
54	326920.0
55	302780.0
56	277463.5
57	251748.0
58	222613.0
59	192500.0
60	160278.0
61	129647.0
62	103989.5
63	81794.5
64	61935.5
65	45851.0
66	33966.0
67	25863.5
68	19717.0
69	14476.0
70	10506.0
71	7435.0
72	5176.0
73	3476.5
74	2309.5
75	1544.5
76	1032.0
77	669.0
78	424.5
79	271.0
80	187.5
81	131.0
82	100.0
83	71.5
84	47.0
85	35.0
86	27.0
87	17.5
88	11.0
89	7.0
90	3.5
91	2.0
92	2.0
93	1.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	4.2962424748955357E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	9.692742168971635E-4
18-19	0.0037775497858532694
20-21	0.0
22-23	0.001309988088016318
24-25	6.131841510449706E-4
26-27	0.013688633857040227
28-29	0.0075382364849177035
30-31	7.038447255504565E-4
32-33	0.006822092821205422
34-35	0.004777663888125167
36-37	0.00205173935275841
38-39	0.001530860738985417
40-41	0.003818904641467697
42-43	0.008427361743772156
44-45	0.009806682762876703
46-47	0.008630268806679222
48-49	0.007907329660248773
50-51	0.03341181315821834
52-53	0.012723241432861514
54-55	8.58214939244962E-5
56-57	0.008060739259353852
58-59	0.0062120326722558995
60-61	1.3569394384359233E-4
62-63	0.005210210844833304
64-65	0.0023857709727342523
66-67	0.023441876880849578
68-69	0.030851525537946886
70-71	0.014836595220481667
72-73	0.010915457570233041
74-75	0.01643387264688325
76-77	0.0022130696907293346
78-79	0.0026742438657401113
80-81	0.001707682044221656
82-83	0.023795884133986457
84-85	7.514764291561651E-4
86-87	0.00919030629225479
88-89	9.223137260700071E-4
90-91	0.0029364699033679375
92-93	0.0011602783734035592
94-95	7.379600696515039E-4
96-97	0.0029701096453965373
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	188.0
20-21	672.0
22-23	1426.0
24-25	2868.0
26-27	4658.0
28-29	9697.0
30-31	19504.0
32-33	25120.0
34-35	35748.0
36-37	53331.0
38-39	64877.0
40-41	69098.0
42-43	77250.0
44-45	88968.0
46-47	89448.0
48-49	83945.0
50-51	78682.0
52-53	77938.0
54-55	84731.0
56-57	88871.0
58-59	88836.0
60-61	88339.0
62-63	87453.0
64-65	91817.0
66-67	97388.0
68-69	97292.0
70-71	96585.0
72-73	92850.0
74-75	95209.0
76-77	96672.0
78-79	102702.0
80-81	103856.0
82-83	102736.0
84-85	103307.0
86-87	110412.0
88-89	118437.0
90-91	126556.0
92-93	139135.0
94-95	168904.0
96-97	367991.0
98-99	630271.0
100-101	5579455.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.2908241039013
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.0700453387067	72.47356609790126
2	7.811467959282546	12.856242716611616
3	2.225066370374047	5.493076359118703
4	0.8583220455407086	2.825281138963648
5	0.4007191039084553	1.6487752647401817
6	0.2219914898694522	1.0960717587246045
7	0.12364293154551707	0.7122275112051997
8	0.08460605565638886	0.5569841635315823
9	0.054574222930005356	0.4041862001766151
>10	0.14766171585791638	1.7634939876043205
>50	0.0015035429317542008	0.08383216910469042
>100	3.550726016056807E-4	0.054817256678712796
>500	2.2075446922006003E-5	0.012098600753027542
>1k	2.2075348018587582E-5	0.01934677488583946
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0478640182672038E-5
2	0.0	4.191456073068815E-5	0.0	0.0	1.0478640182672038E-5
3	0.0	4.191456073068815E-5	0.0	0.0	1.0478640182672038E-5
4	0.0	1.0478640182672039E-4	0.0	0.0	1.0478640182672038E-5
5	0.0	1.0478640182672039E-4	0.0	0.0	1.0478640182672038E-5
6	0.0	1.0478640182672039E-4	0.0	0.0	2.0957280365344076E-5
7	0.0	1.0478640182672039E-4	0.0	0.0	2.0957280365344076E-5
8	0.0	1.1526504200939243E-4	0.0	0.0	2.0957280365344076E-5
9	0.0	2.5148736438412894E-4	0.0	0.0	2.0957280365344076E-5
10-11	1.0478640182672038E-5	2.5148736438412894E-4	0.0	0.0	2.0957280365344076E-5
12-13	1.0478640182672038E-5	2.5148736438412894E-4	0.0	0.0	2.0957280365344076E-5
14-15	1.0478640182672038E-5	3.300771657541692E-4	0.0	0.0	3.143592054801611E-5
16-17	1.0478640182672038E-5	3.457951260281773E-4	0.0	5.239320091336019E-6	6.811116118736826E-5
18-19	1.0478640182672038E-5	3.7723104657619343E-4	0.0	1.0478640182672038E-5	7.335048127870428E-5
20-21	1.0478640182672038E-5	4.2438492739821755E-4	0.0	2.0957280365344076E-5	7.335048127870428E-5
22-23	1.0478640182672038E-5	4.7153880822024173E-4	0.0	2.0957280365344076E-5	7.858980137004029E-5
24-25	1.0478640182672038E-5	4.924960885855858E-4	0.0	3.143592054801612E-5	8.38291214613763E-5
26-27	1.0478640182672038E-5	5.134533689509299E-4	0.0	1.0478640182672039E-4	8.38291214613763E-5
28-29	1.0478640182672038E-5	5.239320091336019E-4	0.0	3.6675240639352135E-4	8.38291214613763E-5
30-31	1.0478640182672038E-5	5.710858899556262E-4	0.0	0.0014565309853914134	8.38291214613763E-5
32-33	1.0478640182672038E-5	5.920431703209702E-4	0.0	0.0033741221388203964	9.430776164404834E-5
34-35	1.0478640182672038E-5	7.282654926957067E-4	0.0	0.006114286546589134	1.1526504200939243E-4
36-37	1.0478640182672038E-5	8.697271351617792E-4	0.0	0.010379093100936654	1.1526504200939243E-4
38-39	1.0478640182672038E-5	9.483169365318195E-4	0.0	0.01733167086213955	1.2574368219206447E-4
40-41	1.0478640182672038E-5	0.0010426246981758678	0.0	0.02460908646900528	1.4670096255740855E-4
42-43	1.0478640182672038E-5	0.001089778578997892	0.0	0.031414963267650774	1.4670096255740855E-4
44-45	1.0478640182672038E-5	0.0012102829410986205	0.0	0.039237268164015446	1.4670096255740855E-4
46-47	1.0478640182672038E-5	0.0013203086630166768	0.0	0.04797121475627259	1.4670096255740855E-4
48-49	1.0478640182672038E-5	0.0013360266232906848	0.0	0.0567575545494431	1.4670096255740855E-4
50-51	1.0478640182672038E-5	0.0014984455461221015	0.0	0.06585825354809377	1.5194028264874456E-4
52-53	1.0478640182672038E-5	0.0015194028264874455	0.0	0.0753623801937773	1.624189228314166E-4
54-55	1.0478640182672038E-5	0.0015246421465787815	0.0	0.08446307919242796	1.676582429227526E-4
56-57	1.0478640182672038E-5	0.0015403601068527896	0.0	0.0915937938367363	1.676582429227526E-4
58-59	1.0478640182672038E-5	0.0015613173872181336	0.0	0.09864591867967457	1.676582429227526E-4
60-61	1.0478640182672038E-5	0.00159275330776615	0.0	0.10601764204818435	1.7813688310542466E-4
62-63	1.0478640182672038E-5	0.001603231947948822	0.0	0.11302261301030062	1.9909416347076874E-4
64-65	1.0478640182672038E-5	0.0016870610694101982	0.0	0.11980753252858076	1.9909416347076874E-4
66-67	1.0478640182672038E-5	0.0016975397095928702	0.0	0.1269696830934371	2.0433348356210476E-4
68-69	1.0478640182672038E-5	0.0017342149502322223	0.0	0.1337650812518999	2.0957280365344077E-4
70-71	1.0478640182672038E-5	0.0017604115506889024	0.0	0.14003130808113778	2.1481212374477678E-4
72-73	1.0478640182672038E-5	0.0017813688310542464	0.0	0.14471002092270085	2.5148736438412894E-4
74-75	1.0478640182672038E-5	0.0018180440716935988	0.0	0.14847185274828012	2.5148736438412894E-4
76-77	1.0478640182672038E-5	0.0019228304735203192	0.0	0.15203982973047994	2.5148736438412894E-4
78-79	1.0478640182672038E-5	0.0019804629945250154	0.0	0.1543870451313985	2.5148736438412894E-4
80-81	1.0478640182672038E-5	0.0019909416347076874	0.0	0.15525677226656026	2.5672668447546495E-4
82-83	1.0478640182672038E-5	0.0020066595949816955	0.0	0.15575450767523719	2.72444644749473E-4
84-85	1.0478640182672038E-5	0.0020118989150730315	0.0	0.15600599503962131	2.72444644749473E-4
86-87	1.0478640182672038E-5	0.0020433348356210476	0.0	0.15617365328254407	2.82923284932145E-4
88	1.0478640182672038E-5	0.002148121237447768	0.0	0.15617365328254407	2.82923284932145E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10305	0.0	45.438335	1
GTATCAA	17845	0.0	33.666084	1
ATCAACG	20305	0.0	28.74318	3
TCAACGC	20335	0.0	28.700474	4
CAACGCA	20685	0.0	28.21485	5
AACGCAG	21630	0.0	27.020819	6
TATCAAC	22420	0.0	26.404627	2
ACGCAGA	25175	0.0	23.16608	7
CGCAGAG	25870	0.0	22.527563	8
GTACATG	25745	0.0	21.451729	1
TACATGG	26250	0.0	20.768288	2
ACATGGG	26570	0.0	19.888771	3
GCAGAGT	30745	0.0	18.710775	9
CATGGGG	20405	0.0	18.23481	4
GAGTACT	16740	0.0	18.133299	12-13
CAGAGTA	30210	0.0	17.001095	10-11
AGAGTAC	27055	0.0	16.967083	10-11
GTACTTT	19185	0.0	15.8448305	14-15
GTGGTAT	6190	0.0	15.601766	1
GAGTACA	19010	0.0	15.350613	1
>>END_MODULE