FastQCFastQC Report
Fri 27 May 2016
SRR2140289_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140289_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6700742
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA80800.038.4243161
GTATCAA135750.031.657571
TCAACGC139800.029.4323484
ATCAACG140550.029.304863
CAACGCA143900.028.4493485
AACGCAG149200.027.4944616
TATCAAC154950.027.0911032
ACGCAGA174400.023.5454717
CGCAGAG178600.022.9452278
GTACATG178350.022.8142641
TACATGG179700.022.0877862
ACATGGG182300.021.0660063
GCAGAGT213500.019.0192579
GAGTACT111850.018.78598212-13
CATGGGG148400.018.1203774
ACCGAAC9650.017.227798
AGAGTAC185700.016.8757410-11
CAGAGTA208350.016.6968810-11
GTGGTAT46400.016.212781
GTACTTT132700.015.866219514-15
Produced by FastQC (version 0.11.5)