##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140289_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6700742
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.068313777787594	33.0	31.0	34.0	30.0	34.0
2	32.20279709321744	33.0	31.0	34.0	30.0	34.0
3	32.10918089369804	33.0	31.0	34.0	30.0	34.0
4	35.98500733202383	37.0	35.0	37.0	35.0	37.0
5	35.8558713348462	37.0	35.0	37.0	35.0	37.0
6	35.91176036922478	37.0	35.0	37.0	35.0	37.0
7	35.86555384463392	37.0	35.0	37.0	35.0	37.0
8	35.85782649145423	37.0	35.0	37.0	35.0	37.0
9	37.61636099405111	39.0	37.0	39.0	35.0	39.0
10-11	37.49619019804075	39.0	37.0	39.0	35.0	39.0
12-13	37.471963776548925	39.0	37.0	39.0	34.0	39.0
14-15	38.77344665710156	40.0	38.0	41.0	35.0	41.0
16-17	38.61987411244904	40.0	38.0	41.0	34.0	41.0
18-19	38.688118942648444	40.0	38.0	41.0	34.0	41.0
20-21	38.71398920253752	40.0	38.0	41.0	34.0	41.0
22-23	38.64186951736549	40.0	38.0	41.0	34.0	41.0
24-25	38.58628349767653	40.0	38.0	41.0	34.0	41.0
26-27	38.38229032566762	40.0	38.0	41.0	34.0	41.0
28-29	38.272414997763846	40.0	38.0	41.0	34.0	41.0
30-31	38.15891986440534	40.0	38.0	41.0	33.5	41.0
32-33	37.98494520891757	40.0	37.0	41.0	33.0	41.0
34-35	38.071155343684424	40.0	37.5	41.0	33.0	41.0
36-37	38.04921012430643	40.0	37.0	41.0	33.0	41.0
38-39	38.00391893083014	40.0	37.0	41.0	33.0	41.0
40-41	37.93440735450573	40.0	37.0	41.0	33.0	41.0
42-43	37.99995350957358	40.0	37.0	41.0	33.0	41.0
44-45	38.00630845054472	40.0	37.0	41.0	33.0	41.0
46-47	37.939464218466966	40.0	37.0	41.0	33.0	41.0
48-49	37.85209634620596	40.0	37.0	41.0	33.0	41.0
50-51	37.73518382456592	40.0	37.0	41.0	32.5	41.0
52-53	37.564322363179436	40.0	36.0	41.0	32.0	41.0
54-55	37.41006318791297	39.0	36.0	41.0	32.0	41.0
56-57	37.27285139961623	39.0	36.0	41.0	31.0	41.0
58-59	37.104683216491466	39.0	35.0	41.0	31.0	41.0
60-61	36.91381747224105	39.0	35.0	40.0	31.0	41.0
62-63	36.69468494267163	38.0	35.0	40.0	31.0	41.0
64-65	36.38538453295486	38.0	35.0	40.0	30.5	41.0
66-67	36.08533357622606	37.0	34.5	40.0	30.0	41.0
68-69	35.76726704637272	37.0	34.0	39.5	30.0	41.0
70-71	35.45224261232141	36.0	34.0	39.0	30.0	41.0
72-73	35.1627155552147	36.0	34.0	39.0	29.0	40.0
74-75	34.83428441162597	35.0	34.0	38.5	29.0	40.0
76-77	33.5670374366612	34.5	32.0	36.5	28.0	39.0
78-79	34.26532831334758	35.0	33.5	37.0	29.0	39.0
80-81	34.17544704891925	35.0	34.0	36.5	29.0	39.0
82-83	33.953298946823544	35.0	33.5	36.0	29.0	38.0
84-85	33.72526633552477	35.0	33.0	36.0	29.0	37.0
86-87	33.535574249953854	35.0	33.0	35.0	29.0	37.0
88-89	33.36935749976652	35.0	33.0	35.0	29.0	36.0
90-91	33.24892663851668	35.0	33.0	35.0	29.0	36.0
92-93	33.200294643180186	35.0	33.0	35.0	29.0	36.0
94-95	33.13396600331798	35.0	33.0	35.0	29.0	36.0
96-97	33.17394068145278	35.0	33.0	35.0	29.0	35.0
98-99	33.28273204898366	35.0	33.0	35.0	29.0	35.0
100	32.057218074033614	34.0	31.0	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	11.0
20	55.0
21	247.0
22	1172.0
23	3824.0
24	9876.0
25	20921.0
26	37517.0
27	59174.0
28	85778.0
29	113536.0
30	143638.0
31	178159.0
32	219971.0
33	278569.0
34	371100.0
35	509509.0
36	784428.0
37	1334673.0
38	1642629.0
39	869984.0
40	35970.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.01428737295064	19.998710590558478	13.513219879231286	24.4737821572596
2	15.190854982925773	21.054892129856665	40.168760414891366	23.5854924723262
3	18.691283335798246	25.316275855255487	30.22194490529894	25.77049590364733
4	12.939014813583332	17.317694070298483	38.068649710733524	31.67464140538466
5	13.435422524848741	37.61292406124576	35.555704129482976	13.395949284422532
6	31.243942834987525	37.22077346061078	18.126052308833856	13.409231395567833
7	27.44885566404437	32.14596234267787	22.602183459682525	17.802998533595236
8	25.182420096162485	36.52247765993676	20.691186140281182	17.603916103619568
9	25.71536107493767	16.4570729629644	20.919429519894962	36.90813644220297
10-11	24.82196747763158	26.412567444023367	28.52339337941977	20.242071698925283
12-13	25.25446435633546	23.847739548843993	28.86051276112407	22.03728333369648
14-15	22.321938376376828	25.358348373956197	26.18822661729104	26.13148663237594
16-17	22.13831186109924	28.553880217035044	26.923446002182764	22.384361919682945
18-19	22.132499961756544	27.884218958531783	28.52953719446742	21.453743885244254
20-21	22.801120728271897	27.049781399483575	28.355384913986185	21.79371295825834
22-23	22.686946550557728	26.938975639735407	28.180142744292034	22.193935065414827
24-25	22.460912394227545	27.12496284462312	28.180451740707944	22.23367302044139
26-27	22.43773997690202	27.153877769810236	28.17820742907133	22.230174824216416
28-29	22.33293822331122	27.31371593764122	28.155097760024354	22.1982480790232
30-31	22.486926353537743	27.172485324887973	28.129630405694634	22.210957915879654
32-33	22.284035881463815	27.286861158132368	28.11091769127333	22.318185269130485
34-35	22.453629612927934	27.253515281018153	28.114910493060876	22.177944612993034
36-37	22.495866191327817	27.264241337602797	27.988977316755147	22.25091515431424
38-39	22.41608038487147	27.341882818348846	28.061743021220337	22.180293775559345
40-41	22.49982893457384	27.238118777399233	27.951147089571084	22.31090519845584
42-43	22.41631526133536	27.437604527376745	27.740746970760373	22.405333240527522
44-45	22.525192617284535	27.40277495032354	27.7026134627898	22.369418969602126
46-47	22.524300639314067	27.40374596238262	27.513095881334475	22.55885751696884
48-49	22.429212937252654	27.658718975568107	27.365036110025432	22.547031977153804
50-51	22.40984365202891	27.54766296029002	27.507045464865428	22.53544792281565
52-53	22.59379883133884	27.52586258033428	27.27501728776676	22.605321300560117
54-55	22.534605653077097	27.4271459935219	27.398562281971262	22.639686071429743
56-57	22.594257279776937	27.525317420888268	27.34172823277308	22.538697066561717
58-59	22.469398406566572	27.652513294332433	27.371799037064832	22.506289262036166
60-61	22.630386863832825	27.559365628456746	27.371702706599816	22.43854480111062
62-63	22.661919368204657	27.6588643868538	27.162439559235914	22.51677668570563
64-65	22.576537962501842	27.606370473574053	27.211142670139942	22.605948893784163
66-67	22.669140266048174	27.586568011543072	27.066722717923454	22.6775690044853
68-69	22.40812425757301	27.584987210247508	27.30422289673482	22.702665635444667
70-71	22.500058925547076	27.40584912073188	27.304389224559593	22.78970272916145
72-73	22.59035376242913	27.51151719398081	27.183502189013897	22.71462685457616
74-75	22.42506888897129	27.608441673081913	27.26171389230021	22.704775545646587
76-77	22.61702833939482	27.45449586947811	27.15595435418512	22.772521436941958
78-79	22.48347999788653	27.45946958355751	27.17174609505272	22.885304323503238
80-81	22.587063109246305	27.447317714639063	27.21684757380111	22.748771602313518
82-83	22.468455203420817	27.54796404705624	27.363351043770145	22.620229705752802
84-85	22.626643680345122	27.458635546216637	27.255608600828463	22.65911217260978
86-87	22.500069256553978	27.424079761066896	27.345558888345987	22.730292094033135
88-89	22.54458127998407	27.36477276993619	27.302039855243976	22.78860609483576
90-91	22.58546935750177	27.411622320292693	27.285369975977257	22.71753834622828
92-93	22.639617572768103	27.253256586377972	27.42994459278466	22.67718124806926
94-95	22.381172137356195	27.390827887325802	27.49885493923086	22.729145036087143
96-97	22.41525007610525	27.508834172376744	27.61274528545961	22.463170466058397
98-99	23.650577613348197	29.491455242622106	27.5999828492325	19.257984294797193
100	22.59600800983195	30.46115429559032	21.256623174099182	25.686214520478543
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	702.0
1	682.5
2	1141.0
3	1737.5
4	2369.5
5	3859.0
6	5262.5
7	6354.0
8	7161.0
9	7143.5
10	6514.0
11	5811.5
12	6015.0
13	7758.0
14	12005.5
15	17982.5
16	23286.0
17	27011.5
18	28735.5
19	28471.0
20	27235.5
21	26257.0
22	26543.5
23	28656.5
24	33375.0
25	41249.5
26	51763.0
27	63052.0
28	75005.0
29	88818.5
30	103575.5
31	119432.0
32	136078.5
33	152729.0
34	168034.0
35	181189.0
36	196476.5
37	213939.5
38	226501.0
39	232787.5
40	240129.5
41	250281.0
42	259532.5
43	269032.0
44	279556.5
45	286091.5
46	289109.5
47	291350.5
48	292119.5
49	291715.5
50	287150.0
51	279287.0
52	269314.0
53	257940.5
54	246773.5
55	232264.0
56	215911.0
57	198315.0
58	178579.5
59	157136.5
60	133057.0
61	109619.0
62	88967.5
63	70437.0
64	54794.5
65	42123.0
66	31797.0
67	24490.0
68	18715.0
69	13898.0
70	10399.0
71	7590.5
72	5371.5
73	3791.5
74	2640.0
75	1774.0
76	1175.5
77	784.5
78	519.0
79	334.5
80	215.5
81	133.0
82	95.0
83	66.5
84	43.5
85	31.5
86	26.0
87	21.5
88	14.5
89	8.0
90	5.5
91	4.0
92	3.0
93	2.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	3.7309300969952283E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0010894315883226066
18-19	0.0037234682368012376
20-21	0.0
22-23	0.0013880937823025654
24-25	5.150593269966107E-4
26-27	0.013471882635109206
28-29	0.007450261842703077
30-31	7.857596406159906E-4
32-33	0.006661387567030212
34-35	0.004440023151334276
36-37	0.0020717763122774825
38-39	0.0015834572566268067
40-41	0.0038047185260029503
42-43	0.008328627697350197
44-45	0.009373953381714173
46-47	0.008557636073314884
48-49	0.008431443834550528
50-51	0.03348111166304867
52-53	0.012471639208071221
54-55	6.563419699185348E-5
56-57	0.007711494579689963
58-59	0.006413566915719863
60-61	1.0172340642866498E-4
62-63	0.0047307334239428765
64-65	0.0026429278024913193
66-67	0.023579119124796412
68-69	0.031585776472867755
70-71	0.015929850371841928
72-73	0.011286341404449006
74-75	0.016581888151383232
76-77	0.0020927552084613085
78-79	0.0025281455030114757
80-81	0.0015655780296576188
82-83	0.024186834624515957
84-85	9.88677565650415E-4
86-87	0.009614695590507908
88-89	7.651541066279995E-4
90-91	0.00278360233872452
92-93	9.531149012843011E-4
94-95	7.794255049459421E-4
96-97	0.00274205459029413
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	161.0
20-21	531.0
22-23	1110.0
24-25	2725.0
26-27	3649.0
28-29	7615.0
30-31	15240.0
32-33	19564.0
34-35	27734.0
36-37	41926.0
38-39	49859.0
40-41	51545.0
42-43	57006.0
44-45	65911.0
46-47	66200.0
48-49	62704.0
50-51	59164.0
52-53	57969.0
54-55	63634.0
56-57	65987.0
58-59	65884.0
60-61	64642.0
62-63	63661.0
64-65	66369.0
66-67	71291.0
68-69	71878.0
70-71	71188.0
72-73	66810.0
74-75	69768.0
76-77	70105.0
78-79	74049.0
80-81	75541.0
82-83	73727.0
84-85	74346.0
86-87	79494.0
88-89	85041.0
90-91	91174.0
92-93	100136.0
94-95	115815.0
96-97	257041.0
98-99	435264.0
100-101	3837284.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.58349787608947
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.62152195124902	78.46328353393965
2	6.713006743407929	11.624712102200693
3	1.5075149029643036	3.91577740196949
4	0.5482621739741302	1.8988182710331722
5	0.23623247979942824	1.022691720648857
6	0.12282889690509172	0.6380973320582651
7	0.0784154403248161	0.47526381775714344
8	0.04036313189949508	0.2795824916073801
9	0.033074793144665215	0.2577358153793897
>10	0.09696953168559112	1.2928769506946436
>50	0.0015639773607857173	0.08937165012955949
>100	2.4597728473543914E-4	0.04178891258178361
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.9847440775961825E-5	0.0	0.0	0.0
3	0.0	2.9847440775961825E-5	0.0	1.4923720387980913E-5	0.0
4	0.0	1.193897631038473E-4	0.0	2.9847440775961825E-5	0.0
5	0.0	1.193897631038473E-4	0.0	5.969488155192365E-5	0.0
6	1.193897631038473E-4	1.193897631038473E-4	0.0	8.954232232788548E-5	0.0
7	1.193897631038473E-4	1.193897631038473E-4	0.0	8.954232232788548E-5	0.0
8	1.193897631038473E-4	1.193897631038473E-4	0.0	8.954232232788548E-5	0.0
9	1.193897631038473E-4	1.6416092426779004E-4	0.0	1.3431348349182822E-4	0.0
10-11	1.2685162329783775E-4	1.6416092426779004E-4	0.0	1.8654650484976141E-4	0.0
12-13	1.3431348349182822E-4	1.6416092426779004E-4	0.0	1.9400836504375186E-4	0.0
14-15	1.3431348349182822E-4	2.462413864016851E-4	0.0	1.9400836504375186E-4	0.0
16-17	3.133981281475992E-4	2.537032465956755E-4	0.0	2.0893208543173279E-4	0.0
18-19	3.133981281475992E-4	2.6862696698365645E-4	0.0	2.3131766601370413E-4	0.0
20-21	3.133981281475992E-4	2.8355068737163735E-4	0.0	2.7608882717764687E-4	0.0
22-23	3.133981281475992E-4	3.133981281475992E-4	0.0	3.133981281475992E-4	0.0
24-25	3.133981281475992E-4	3.880167300875037E-4	0.0	3.43245568923561E-4	0.0
26-27	3.133981281475992E-4	4.477116116394274E-4	0.0	4.4024975144543694E-4	0.0
28-29	3.133981281475992E-4	4.477116116394274E-4	0.0	8.804995028908738E-4	0.0
30-31	3.133981281475992E-4	4.850209126093797E-4	0.0	0.002417642702852908	0.0
32-33	3.208599883415896E-4	4.924827728033702E-4	0.0	0.005029293770749567	0.0
34-35	3.283218485355801E-4	5.447157941613033E-4	0.0	0.008558753642507053	0.0
36-37	3.283218485355801E-4	5.969488155192365E-4	0.0	0.013670127875390516	0.0
38-39	3.283218485355801E-4	6.267962562951984E-4	0.0	0.021766246185870163	0.0
40-41	3.283218485355801E-4	6.715674174591411E-4	0.0	0.03039215657012313	0.0
42-43	3.283218485355801E-4	6.86491137847122E-4	0.0	0.03888375347088427	0.0
44-45	3.283218485355801E-4	7.68571599981017E-4	0.0	0.048427472658998064	0.0
46-47	3.283218485355801E-4	8.357283417269311E-4	0.0	0.05870991600631691	0.0
48-49	3.3578370872957056E-4	8.357283417269311E-4	0.0	0.06953707514779707	0.0
50-51	3.880167300875037E-4	9.103469436668357E-4	0.0	0.08037915800966519	0.0
52-53	4.104023106694751E-4	9.103469436668357E-4	0.0	0.09094515204435569	0.0
54-55	4.1786417086346557E-4	9.103469436668357E-4	0.0	0.10034709588878366	0.0
56-57	4.1786417086346557E-4	9.252706640548166E-4	0.0	0.1082044346730556	0.0
58-59	4.1786417086346557E-4	9.252706640548166E-4	0.0	0.11612893019907347	0.0
60-61	4.4024975144543694E-4	9.401943844427976E-4	0.0	0.12431459083188101	0.0
62-63	4.924827728033702E-4	9.625799650247689E-4	0.0	0.13235101426080875	0.0
64-65	5.07406493191351E-4	0.0011491264698745302	0.0	0.14020089118488668	0.0
66-67	5.521776543552937E-4	0.0011565883300685208	0.0	0.1482298527536204	0.0
68-69	5.521776543552937E-4	0.0011789739106504921	0.0	0.15596033991459451	0.0
70-71	5.596395145492843E-4	0.0011789739106504921	0.0	0.16275063269112583	0.0
72-73	5.894869553252461E-4	0.0011789739106504921	0.0	0.16776500274148742	0.0
74-75	5.969488155192365E-4	0.0011789739106504921	0.0	0.1723391230404036	0.0
76-77	6.417199766831792E-4	0.0012162832116204443	0.0	0.1767789298558279	0.0
78-79	6.715674174591411E-4	0.0012386687922024157	0.0	0.17949504696644042	0.0
80-81	6.715674174591411E-4	0.0012386687922024157	0.0	0.18042777949068922	0.0
82-83	6.715674174591411E-4	0.0012386687922024157	0.0	0.18090533854310464	0.0
84-85	7.014148582351029E-4	0.0012685162329783776	0.0	0.18121127481105825	0.0
86-87	7.014148582351029E-4	0.0012983636737543395	0.0	0.1813381264343561	0.0
88	7.014148582351029E-4	0.0012983636737543395	0.0	0.18135305015474407	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8080	0.0	38.424316	1
GTATCAA	13575	0.0	31.65757	1
TCAACGC	13980	0.0	29.432348	4
ATCAACG	14055	0.0	29.30486	3
CAACGCA	14390	0.0	28.449348	5
AACGCAG	14920	0.0	27.494461	6
TATCAAC	15495	0.0	27.091103	2
ACGCAGA	17440	0.0	23.545471	7
CGCAGAG	17860	0.0	22.945227	8
GTACATG	17835	0.0	22.814264	1
TACATGG	17970	0.0	22.087786	2
ACATGGG	18230	0.0	21.066006	3
GCAGAGT	21350	0.0	19.019257	9
GAGTACT	11185	0.0	18.785982	12-13
CATGGGG	14840	0.0	18.120377	4
ACCGAAC	965	0.0	17.22779	8
AGAGTAC	18570	0.0	16.87574	10-11
CAGAGTA	20835	0.0	16.69688	10-11
GTGGTAT	4640	0.0	16.21278	1
GTACTTT	13270	0.0	15.8662195	14-15
>>END_MODULE